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Integrated indicator framework and methodology for monitoring and assessment of hazardous substances and their effects in the marine environment ArchiMer
Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil.
Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for...
Tipo: Text Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf
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Platinum in sediments and mussels from the northwestern Mediterranean coast: temporal and spatial aspects ArchiMer
Abdou, Melina; Schafer, Jorg; Hu, Ruoyu; Gil-diaz, Teba; Garnier, Cedric; Brach-papa, Christophe; Chiffoleau, Jean-francois; Charmasson, Sabine; Giner, Franck; Dutruch, Lionel; Blanc, Gerard.
Platinum (Pt) is considered a Technology Critical Element (TCE) and an emerging metallic contaminant with increasing release into the environment. Gaps in knowledge and understanding of environmental levels, fate and effects of Pt still exist, especially in the marine environment. This work presents Pt concentrations in the northwestern Mediterranean coast including: (i) temporal variability from sediment cores and farmed mussels in the Toulon Bay (historically affected by intense human activities) and (ii) spatial distribution from recent wild mussels collected along ∼ 300 km coastline with contrasting ecosystems (including natural reserves), quantified using voltammetry and inductively coupled plasma-mass spectrometry. The historical (> 100 years)...
Tipo: Text Palavras-chave: Platinum; Sediments; Mussels; Biomonitoring; Mediterranean coast.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00460/57134/59055.pdf
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An Assessment of Environmental Metabarcoding Protocols Aiming at Favoring Contemporary Biodiversity in Inventories of Deep-Sea Communities ArchiMer
Brandt, Miriam; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Maignien, Lois; De Vargas, Colomban; Wincker, Patrick; Poulain, Julie; Zeppilli, Daniela; Arnaud-haond, Sophie.
The abyssal seafloor covers more than 50% of planet Earth and is a large reservoir of still mostly undescribed biodiversity. It is increasingly targeted by resource-extraction industries and yet is drastically understudied. In such remote and hard-to-access ecosystems, environmental DNA (eDNA) metabarcoding is a useful and efficient tool for studying biodiversity and implementing environmental impact assessments. Yet, eDNA analysis outcomes may be biased toward describing past rather than present communities as sediments contain both contemporary and ancient DNA. Using commercially available kits, we investigated the impacts of five molecular processing methods on eDNA metabarcoding biodiversity inventories targeting prokaryotes (16S), unicellular...
Tipo: Text Palavras-chave: Environmental metabarcoding; RNA versus DNA; Extracellular DNA; Deep-sea biodiversity; Benthic ecology; Biomonitoring; Method testing.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74165/73774.pdf
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Bioindicators of pollutant exposure in the northwestern Mediterranean sea ArchiMer
Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois.
Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),...
Tipo: Text Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf
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Trace metals assessment in water, sediment, mussel and seagrass species - Validation of the use of Posidonia oceanica as a metal biomonitor ArchiMer
Lafabrie, Céline; Pergent, G; Kantin, Roger; Pergent Martini, C; Gonzalez, Jean-louis.
The accumulation of trace metals (Cd, Co, Cr, Hg, Ni and Pb) was measured in water, sediment, the mussel Mytilus galloprovincialis and the seagrass Posidonia oceanica. Samples were collected in three locations of the north-western Mediterranean (Canari, Livorno and Porto-Torres) which present different levels and sources of human impact. Analyses in the different compartments (water, sediment, M. galloprovincialis and P. oceanica) have allowed to identify Canari as the most Cd, Co, Cr and Ni contaminated site; Livorno as the most Hg contaminated and Porto-Torres as the most Pb contaminated. Furthermore, for the first time, metal concentrations found in P. oceanica have been compared with those found in the water column, in the sediment and in the...
Tipo: Text Palavras-chave: Mediterranean sea; Marine organisms; Sediment; Seawater; Biomonitoring; Metal contamination.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3106.pdf
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Organophosphorous Pesticides: Their Effects on Biosentinel Species and Humans. Control and Application in Chile International Journal of Morphology
Espinoza-Navarro,Omar; Ponce-LaRosa,Carlos; Bustos-Obregón,Eduardo.
The environment is negatively affected by the increasing accumulation of both natural and man-made waste and by-products. Organophosphorous pesticides -malathion, diazinon and methamidophos- are used worldwide in pest control. The aim of this report is to review the effects of organophosphates on the male reproductive tract of mice, rats and earthworms, and to evaluate their projection into the human population. Assessing failures in the male reproductive system is an excellent in vivo biomarker to determine the level of toxicity of suspected pollutants. In rodents organophosphates cause decreased testicular weight and sperm density, abnormal tubular plugging and increased teratozoospermia. In earthworms they cause a significant decrease in body weight and...
Tipo: Journal article Palavras-chave: Organophosphates; Cytotoxicity; Biomonitoring; Reproductive Health; Occupational Exposure.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300041
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Application of rapid bioassessment protocols (RBP) for benthic macroinvertebrates in Brazil: comparison between sampling techniques and mesh sizes Neotropical Entomology
Buss,Daniel F.; Borges,Erika L..
This study is part of the effort to test and to establish Rapid Bioassessment Protocols (RBP) using benthic macroinvertebrates as indicators of the water quality of wadeable streams in south-east Brazil. We compared the cost-effectiveness of sampling devices frequently used in RBPs, Surber and Kick-net samplers, and of three mesh sizes (125, 250 and 500 µm). A total of 126,815 benthic macroinvertebrates were collected, representing 57 families. Samples collected with Kick method had significantly higher richness and BMWP scores in relation to Surber, but no significant increase in the effort, measured by the necessary time to process samples. No significant differences were found between samplers considering the cost/effectiveness ratio. Considering mesh...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental assessment; Aquatic insect; Freshwater ecology; Biomonitoring; Bioindicator.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000300007
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Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil Neotropical Ichthyology
Loro,Vania Lucia; Murussi,Camila; Menezes,Charlene; Leitemperger,Jossiele; Severo,Eduardo; Guerra,Luciana; Costa,Maiara; Perazzo,Giselle Xavier; Zanella,Renato.
Due to intense agricultural activity in the rio Uruguai (South Brazil), there is the potential for aquatic contamination by agrochemicals. In this region, there are many reservoirs to meet the water demand for rice fields, forming lentic environments. In line with this information, the aim of this study was to show a comparative analysis of some biomarkers, such as lipid peroxidation (TBARS), gluthatione S-transferase (GST), non-protein thiols (NPSH), amino acids (AA) and piscine micronucleus tests (MNE) in Astyanax jacuhiensis from lentic and lotic environments in the middle rio Uruguai region, comparing warm and cold seasons. Eight pesticides were found in water samples, with propoxur having the highest concentration found in both environments and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Lotic and lentic environments; Pampa; Pesticides.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300569
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Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas Neotropical Ichthyology
Vieira,Carlos Eduardo Delfino; Almeida,Mayara da Silva; Galindo,Bruno Ambrssio; Pereira,Lindalva; Martinez,Claudia Bueno dos Reis.
Aquatic ecosystems in areas with intense agricultural activity are subject to pesticide contamination, which may compromise the health of the fish. In order to verify the quality of the water and the possible effects of pesticides on fish, a method that combines different biomarker responses into an index named "integrated biomarker response" (IBR) was applied using the biological alterations in the Neotropical fish Astyanax altiparanae. Fish were maintained in situ at five sites along a stream that runs in an agricultural area and in a stream within a forest fragment, considered a reference site. After seven days of exposure the following alterations were observed in fish confined at experimental sites: increased activity of glutathione-S-transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax altiparanae; Biomonitoring; Biotransformation; Genotoxicity; Pesticides.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000100153
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Rol de las macroalgas en el biomonitoreo de la contaminación en «Marchica», la laguna de Nador Phyton
Ben Chekroun,K; Moumen,A; Rezzoum,N; Sánchez,E; Baghour,M.
The Aquatic system contamination by organic pollutants and heavy metals is one of the most serious problems that might face the environment and organisms as a whole. Interestingly, the accumulation of these pollutants is due to the anthropogenic activity, namely the intensive use of fertilizers to obtain high yields in crop production. In this paper, we compared the levels of chlorophylls, proteins, dry weight and sucrose in some red (Alsidium sp. and Gracilaria sp.) and green algae (Ulva sp.) collected from Mohandis, near Kariat and Bou Areg. Our results indicate that the algae collected nearby Bou Areg, an area well-known for its high agricultural activity, contained high levels of pigments, proteins and sugars. From the chlorophyll results, we conclude...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algae; Biomonitoring; Carbohydrates; Nador lagoon; Phytoremediation; Pigments; Pollution; Proteins.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572013000100005
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Estudo anatômico foliar do clone híbrido 4430 de Tradescantia: alterações decorrentes da poluição aérea urbana Rev. Bras. Bot.
ALVES,EDENISE SEGALA; GIUSTI,PAULA MATTOS; DOMINGOS,MARISA; SALDIVA,PAULO H.N.; GUIMARÃES,ELIANE T.; LOBO,DÉBORA J.A..
Estudos epidemiológicos mostram uma relação entre a poluição aérea em centros urbanos e a taxa de doenças respiratórias na sua população. Em São Paulo, a concentração dos principais poluentes aéreos é monitorada pela CETESB porém, tais dados não dão idéia do risco ao qual a população está sujeita; plantas bioindicadoras podem dar idéia deste risco. O clone híbrido 4430 de Tradescantia vem sendo utilizado em bioensaios (Trad-MCN, Trad-SH) que avaliam o efeito genotóxico de várias substâncias. Não há registros das potencialidades de bioindicação relativas à estrutura das folhas do clone. Este estudo objetivou estabelecer o padrão estrutural da folha do clone e verificar se existem características anatômicas afetadas pela poluição, que podem ser utilizadas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urban air pollution; Tradescantia; Clone 4430; Leaf anatomy; Biomonitoring.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500012
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The effects of heavy metals on the incidence of morphological deformities in Chironomidae (Diptera) Rev. Bras. Zool.
Deliberalli,Wanessa; Cansian,Rogério L.; Pereira,Albanin A. M.; Loureiro,Rafael C.; Hepp,Luiz U.; Restello,Rozane M..
ABSTRACT Streams in urban areas are strongly impacted by the input of organic matter and metals, for instance copper (Cu) and zinc (Zn). These metals are essential for the aquatic biota, but when absorbed in excess they are toxic. In Chiro nomidae larvae, the deleterious effects of heavy metals can be ascertained by analyzing the morphological deformities of the larval mentum, a structure of the oral cavity. In this study, we evaluated I) the bioavailability of Cu and Zn in urban stream sediments and II) the relationship between Cu and Zn concentrations and the incidence of deformities in the mentum of Chironomus larvae. Chironomid flies were collected from four locations in two streams at an urban area in southern Brazil. They were identified and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Copper; Environmental quality; Mentum; Zinc.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100316
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Effects of agricultural and urban impacts on macroinvertebrates assemblages in streams (Rio Grande do Sul, Brazil) Rev. Bras. Zool.
Hepp,Luiz Ubiratan; Milesi,Silvia V.; Biasi,Cristiane; Restello,Rozane M..
This study evaluates the effects of agricultural and urban activities on the structure and composition of benthic communities of streams in the state of Rio Grande do Sul, Brazil. Benthic macroinvertebrates were collected in streams influenced by urbanization and agriculture and in streams with no anthropogenic disturbances (reference streams). Organism density was superior in urban streams when compared with streams in the other two areas. The taxonomic richness and Shannon diversity index were higher in reference streams. The benthic fauna composition was significantly different among land uses. The classification and ordination analyses corroborated the results of variance analyses demonstrating the formation of clusters corresponding to streams with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Environmental quality; Land uses.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000100016
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The influence of habitat integrity and physical-chemical water variables on the structure of aquatic and semi-aquatic Heteroptera Rev. Bras. Zool.
Dias-Silva,Karina; Cabette,Helena S. R.; Juen,Leandro; Jr,Paulo De Marco.
This work aimed to assess the effect of certain physical-chemical variables and the Habitat Integrity Index (HII) have on an aquatic and semi-aquatic heteropteran community. We collected in five streams (from 1st to 4th order) that differed in habitat integrity, in order to test 1) whether heteropteran richness decreases with the Habitat Integrity Index; and 2) whether richness responds to alterations in water physical-chemical variables, since these influence community structure. In each stream, linear transects of 100 m were demarcated. A total of 1425 specimens from 10 families, 30 genera and 67 morphospecies were collected. Species richness was correlated with the Habitat Integrity Index (HII), showing a positive relationship only for Gerromorpha. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic insect; Biomonitoring; Cerrado; Gerromorpha; Nepomorpha.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600013
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Multimetric index for assessing ecological condition of running waters in the upper reaches of the Piabanha-Paquequer-Preto Basin, Rio de Janeiro, Brazil Rev. Bras. Zool.
Baptista,Darcilio F.; Souza,Roberta S. G. de; Vieira,Carla A.; Mugnai,Riccardo; Souza,Ana S.; Oliveira,Renata Bley S. de.
The objective of this study was to develop a rapid multimetric index using benthic macrofauna as bioindicators of ecological conditions for the upper reaches of the Piabanha-Paquequer-Preto Basin located in the state of Rio de Janeiro, Brazil. A total of 33698 macroinvertebrates were collected in 27 sites. Benthic macroinvertebrates were sampled using the multi-habitat method that consists in sampling 20 m² of substrate collected in approximate proportion to the representation of all major habitat types in the reach. A subsampling procedure was used. The main steps followed to develop and test the index were: exclusion of unsuitable metrics using box-whisker plots, considering the degree of overlapping among interquartile limits (1º-3º) and confirmed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Conservation; River ecosystems; Macroinvertebrates.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000500010
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Two practical approaches to monitoring the zooplanktonic community at Lago Grande do Curuai, Pará, Brazil Acta Amazonica
GOMES,Leonardo Fernandes; VIEIRA,Ludgero Cardoso Galli; BONNET,Marie Paule.
The use of substitute groups in biomonitoring programs has been proposed to minimize the high financial costs and time for samples processing. The objectives of this study were to evaluate the correlation between (i) the spatial distribution among the major zooplankton groups (cladocerans, copepods, rotifers, and testaceans protozoa), (ii) the data of density and presence/absence of species, and (iii) the data of species, genera, and families from samples collected in the Lago Grande do Curuai, Pará, Brazil. A total of 55 sample of the zooplanktonic community was collected, with 28 samples obtained in March and 27 in September, 2013. The agreement between the different sets of data was assessed using Mantel and Procrustes tests. Our results indicated high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Cladoceran; Copepod; Rotifer; Testate amoebae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672015000300293
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Micronúcleos em tétrades de Tradescantia pallida (Rose) Hunt. cv. purpúrea Boom: alterações genéticas decorrentes de poluição aérea urbana - DOI: 10.4025/actascibiolsci.v30i3.5017 Biological Sciences
Andrade Júnior, Sizenando José de; UESC; Santos Júnior, José Cleub Silva; UNEB; Oliveira, Jesiane da Luz; UNEB; Cerqueira, Eneida de Moraes Marcílio; UEFS; Meireles, José Roberto Cardoso; UEFS.
A atmosfera urbana contém uma mistura de poluentes que inclui substâncias mutagênicas e carcinogênicas. Associação entre estas substâncias e problemas respiratório e cardiovascular, além do aumento de determinados tipos de cânceres, é estabelecida na literatura. Assim, o biomonitoramento é importante para avaliar o potencial genotóxico da atmosfera urbana. O Teste de Micronúcleo em Tradescantia (Trad-MCN) é um dos bioensaios usados para detecção de efeitos genotóxicos. Este teste é baseado na formação de micronúcleos resultante de quebra cromossômica na meiose das células-mãe do grão de pólen de inflorescências de Tradescantia ssp. Neste estudo, Trad-MCN foi utilizado para avaliar o potencial mutagênico do ar atmosférico em duas áreas de tráfego...
Palavras-chave: 2.00.00.00-6 Ciências Biológicas Tradescantia pallida; Micronúcleo; Poluição atmosférica; Biomonitoramento; Genotoxicidade. Tradescantia pallida; Micronuclei; Urban air pollution; Biomonitoring; Genotoxicity.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5017
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Taxonomic and non-taxonomic responses of benthic macroinvertebrates to metal toxicity in tropical reservoirs. The case of Cantareira Complex, São Paulo, Brazil Anais da ABC (AABC)
BEGHELLI,FREDERICO G.S.; CETRA,MAURÍCIO; MARCHESE,MERCEDES; LÓPEZ-DOVÁL,JÚLIO CÉSAR; ROSA,ANDRÉ H.; POMPÊO,MARCELO L. M.; MOSCHINI-CARLOS,VIVIANE.
Abstract Benthic macroinvertebrates are organisms that are recognized as water quality bio-indicators. A wide variety of indices and metrics have been shown to respond to a variety of anthropogenic impacts, usually under a general condition of environmental impairment. The absence of a clear distinction in the relations between specific pollutants and biotic variables is very common and can lead to biased interpretation of biomonitoring. The aims of this research were to test taxonomic and non-taxonomic responses to specific environmental conditions instead to general conditions. For this purpose, we estimated the theoretical toxicity by comparing toxicity values published by EPA with metal concentrations in water and sediments. Then we tested the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio-indicator; Biomonitoring; Chironomid; Oligochaetes; Toxicity; Metal.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000300701
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Behavioral response of Biomphalaria glabrataexposed to a sublethal concentration of Euphorbia miliivar. hislopii latex Anais da ABC (AABC)
ALBERTO-SILVA,ANNA CARLA; CUNHA,RODOLFO A.; COSTA,VALDIR A. DA; SANTOS,EVERTON GUSTAVO N. DOS; VASCONCELLOS,MAURÍCIO C. DE; MELLO-SILVA,CLÉLIA CHRISTINA; SANTOS,CLÁUDIA P..
Abstract The Euphobia milii var. hislopii latex has been tested in the control of schistosomiasis but its action in the locomotor activity of Biomphalaria glabrata is unknown. The objective of this work was to study the locomotor and reproductive behaviors of B. glabrata exposed to E. milii var. hislopii latex. For this, 96 snails were individually exposed to the latex (LC50 - 0.53 mg / L) for 24 hours. The specimens were submitted to biomonitoring for image analysis to record the locomotor parameters at different times: before exposure (control), one day post exposure (group 1 d-p-e) and 30 days post exposure (group 30 d-p-e). The reproductive parameters were recorded weekly for 10 weeks. All locomotor activities of group 1 d-p-e decreased significantly....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Behavior; Biomonitoring; Biomphalaria glabrata; Control of schistosomiasis; Euphorbia milii var.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100716
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Air quality assessment in different urban areas from Rio Grande do Sul state, Brazil, using lichen transplants Anais da ABC (AABC)
KOCH,NATÁLIA MOSSMANN; LUCHETA,FABIANE; KÄFFER,MÁRCIA ISABEL; MARTINS,SUZANA MARIA DE AZEVEDO; VARGAS,VERA MARIA FERRÃO.
Abstract Based on the need to monitor air pollution and on the importance of doing it with biological organisms, the present work had as main objective to assess air quality in urban areas through lichen transplants. Besides, we also tested whether there were differences among the studied sites, aiming to point out the main pollution characteristics of the areas. The monitoring was carried out in seven cities, which varied from mainly rural to mainly industrial, in Rio Grande do Sul, southern Brazil. The foliose lichen Parmotrema tinctorum was used as the biomonitor species. Physiological tests and contents of sulphur and heavy metals were measured in the lichen thalli. Other pollutants assessed through air samplers were available for some cities, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Air pollution; Biomonitoring; Greenhouse gases; Heavy metals; Lichenized fungi; Particulate matter.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502233
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