SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Jiang,Tao; Chen,Zhao-Hui; Chen,Zhe; Tan,Dan.

The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological...

p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA

Palavras-chave: SULF2; Cervical cancer; ERK/AKT; Proliferation; Apoptosis.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200608

Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P..

The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both...

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer

Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006

Song,Linlin; Wang,Linlin; Pan,Xiaoli; Yang,Caihong.

Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of...

Epidemiological and functional implications of molecular variants of human papillomavirus

Palavras-chave: LncRNA; OIP5-AS1; Cervical cancer; MiR-143-3p; ROCK1.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100608

Sichero,L.; Villa,L.L..

Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional...

The contribution of Latin American research to HPV epidemiology and natural history knowledge

Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002

Sichero,L.; Picconi,M.A.; Villa,L.L..

Our aim was to review the major contributions of studies conducted in different Latin American (LA) countries to the field of human papillomavirus (HPV) epidemiology, natural history, risk of disease, and prevention strategies, mainly in the uterine cervix. Although cytological screening is established in several countries in LA, incidence and mortality rates from cervical cancer (CC) are still extremely high. Finally, data from large cohort studies conducted in LA countries provided seminal data to propose primary and secondary prevention modalities: the HPV vaccine has been introduced in the national immunization programs of several LA countries and multiple screening experiences using HPV testing are under evaluation in the region.

Genetic susceptibility to HPV infection and cervical cancer

Palavras-chave: HPV; Latin America; Immunization; Cervical cancer; Natural history; Screening.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200302

Maciag,P.C.; Villa,L.L..

Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV...

The effect of performing corrections on reported uterine cancer mortality data in the city of São Paulo

Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017

Antunes,J.L.F.; Wünsch-Filho,V..

Reports of uterine cancer deaths that do not specify the subsite of the tumor threaten the quality of the epidemiologic appraisal of corpus and cervix uteri cancer mortality. The present study assessed the impact of correcting the estimated corpus and cervix uteri cancer mortality in the city of São Paulo, Brazil. The epidemiologic assessment of death rates comprised the estimation of magnitudes, trends (1980-2003), and area-level distribution based on three strategies: i) using uncorrected death certificate information; ii) correcting estimates of corpus and cervix uteri mortality by fully reallocating unspecified deaths to either one of these categories, and iii) partially correcting specified estimates by maintaining as unspecified a fraction of deaths...

Radiotherapy modulates expression of EGFR, ERCC1 and p53 in cervical cancer

Palavras-chave: Uterine cancer; Cervical cancer; Mortality; Statistical models; Socioeconomic factors; São Paulo.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800012

de Almeida,V.H.; de Melo,A.C.; Meira,D.D.; Pires,A.C.; Nogueira-Rodrigues,A.; Pimenta-Inada,H.K.; Alves,F.G.; Moralez,G.; Thiago,L.S.; Ferreira,C.G.; Sternberg,C..

Cervical cancer is a public health problem and the molecular mechanisms underlying radioresistance are still poorly understood. Here, we evaluated the modulation of key molecules involved in cell proliferation, cell cycle and DNA repair in cervical cancer cell lines (CASKI and C33A) and in malignant tissues biopsied from 10 patients before and after radiotherapy. The expression patterns of epidermal growth factor receptor (EGFR), excision repair cross-complementation group 1 (ERCC1) and p53 were evaluated in cancer cell lines by quantitative PCR and western blotting, and in human malignant tissues by immunohistochemistry. The mutation status of TP53 gene was evaluated by direct sequencing. Among cell lines, absent or weak modulations of EGFR, ERCC1 and p53...

Analysis of the application values of different combination schemes of liquid-based cytology and high-risk human papilloma virus test in the screening of high-grade cervical lesions

Palavras-chave: Cervical cancer; Radiotherapy; Epidermal growth factor receptor (EGFR); P53; Excision repair cross-complementation group 1 (ERCC1).

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100608

Wang,Jian.

The aim of this study was to explore the value of different combination schemes of liquid-based cytology (LBC) and high-risk human papilloma virus (HPV) test in the screening of high-grade (≥CIN 2) cervical lesions. From 5727 women who had undergone examinations with LBC and high-risk HPV test, 1884 patients with positive results of either or both LBC and HPV test were included in this study and underwent cervical biopsy. Based on the pathological examination results, comparisons of the assessment indicators of all diagnostic tests were made, and the application values of LBC and high-risk HPV test and different combination schemes of the two in the screening of high-grade (≥CIN II) cervical lesions were estimated. Compared with the single test method, the...

High incidence of oncogenic HPV genotypes found in women from Southern Brazil

Palavras-chave: Liquid-based cytology; Human papilloma virus; Cervical cancer; ROC curve; High-grade.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100601

Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.; Collares,Tiago; Seixas,Fabiana K..

Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p <...

Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil

Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043

Fernandes,Thales Allyrio Araújo de Medeiros; Meissner,Rosely de Vasconcellos; Bezerra,Laelson Freire; Azevedo,Paulo Roberto Medeiros de; Fernandes,José Veríssimo.

We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified,...

Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Palavras-chave: Human papillomavirus; Cervical cancer; Epidemiology; Prevalence; Risk factors.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300031

Santos,Carla R.; Franciscatto,Laura G.; Barcellos,Regina B.; Almeida,Sabrina E. M; Rossetti,Maria Lucia R..

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

Correlation between ebv co-infection and HPV16 genome integrity in Tunisian cervical cancer patients

Palavras-chave: HPV; Cervical cancer; FTA elute; Molecular detection.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100047

Kahla,Saloua; Oueslati,Sarra; Achour,Mongia; Kochbati,Lotfi; Chanoufi,Mohamed Badis; Maalej,Mongi; Oueslati,Ridha.

Infection with high risk Human papillomavirus (HR-HPV) is necessary but not sufficient to cause cervical carcinoma. This study explored whether multiple HR-HPV or coinfection with Epstein-Barr virus (EBV) influence the integration status of HPV16 genome. The presence and typing of HPV in a series of 125 cervical specimens were assessed by polymerase chain reaction (PCR) using the specific primers for the HPV L1 region. As for EBV infection, the viral EBNA1 gene was used for its detection through PCR amplification. Disruption of the HPV E2 gene was assessed by amplification of the entire E2 gene with single set of primers, while E2 transcripts were evaluated by a reverse transcription PCR method (RT-PCR). The overall prevalence of HPVDNA was of 81.8% in...

Antineoplastic potential of the aqueous crude extract of Eugenia uniflora L. in human cervical cancer

Palavras-chave: Cervical cancer; Epstein-Barr virus; E2 gene; E2 mRNA; High risk human papillomaviruses.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200039

BJPS

Núñez,Jisette González; Pinheiro,Jordânia dos Santos; Silveira,Gabriel Fernandes; Beckenkamp,Aline; Buffon,Andréia; Bruno,Alessandra Nejar.

Abstract Considering the high prevalence of human cervical cancer and the adverse effects of the available treatments, it is important to develop studies involving plants. Eugenia uniflora L. is a Brazilian native plant widely used in folk medicine and some biological effects have already been described. In this study, we investigated the biologicals effects of the aqueous crude extract of E. uniflora leaves in relation to the viability of human cervical cancer cells (SiHa), non-tumorigenic cells HaCaT and human lymphocytes. Our results demonstrated that different concentrations of E. uniflora’s extract significantly inhibited the viability of the Siha cell line at 24, 48 and 72 hours of treatment, but did not induce significant changes in the HaCat cell...