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Registros recuperados: 30
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DNA barcodes for soil animal taxonomy PAB
Rougerie,Rodolphe; Decaëns,Thibaud; Deharveng,Louis; Porco,David; James,Sam W.; Chang,Chih-Han; Richard,Benoit; Potapov,Mikhail; Suhardjono,Yayuk; Hebert,Paul D.N..
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collembola; Cryptic diversity; DNA barcoding; Oligochaeta; Species identification; Taxonomic crisis; Taxonomic impediment.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800002
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First record of the sedge feeder Bactra verutana Zeller (Lepidoptera: Tortricidae) in Chile based on morphology and DNA barcodes Rev. Bras. entomol.
Vargas,Héctor A.; Vargas-Ortiz,Marcelo.
Abstract The sedge-feeding moth Bactra verutana Zeller, 1875 (Lepidoptera: Tortricidae: Olethreutinae: Bactrini), described from Dallas, Texas, USA, is widespread, recorded throughout much North America, Central and South America, including the Caribbean, and Africa. The species is recorded for the first time from Chile based on specimens collected in the coastal valleys of the Atacama Desert, where its larvae feed on Cyperus corymbosus Rottb. var. subnodosus (Nees & Meyen) Kük. (Cyperaceae). A single DNA barcode haplotype, which is widespread in USA, was found in two Chilean specimens sequenced.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Atacama Desert; Cyperaceae; Cyperus corymbosus; DNA barcoding.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200104
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Two new species of Mycodrosophila (Diptera, Drosophilidae) proposed by molecular and morphological approaches, with a key to American species Rev. Bras. entomol.
Junges,João; Gottschalk,Marco Silva; Loreto,Elgion Lucio da Silva; Robe,Lizandra Jaqueline.
ABSTRACT There are approximately 130 species of MycodrosophilaOldenberg, 1914 worldwide, although only nine species were recorded in American countries so far, three of which are exclusively Nearctic, five exclusively Neotropical and one found in both biogeographic regions (Mycodrosophila projectans). Such a small number of American species is likely a consequence of collecting bias, which favors the capture of frugivorous drosophilids, and to the general absence of Neotropical Mycodrosophila studies in the last 50 years. Here, we describe two commonly sampled species of Mycodrosophila from the Amazonian and Pampa Brazilian biomes, which share morphological similarities with Mycodrosophila neoprojectans and M. projectans, respectively. We compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian forest; Cytochrome oxidase subunit I; DNA barcoding; Mycophagous drosophilids; Pampa biome.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000100030
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Saving our science from ourselves: the plight of biological classification Rev. Bras. entomol.
Ebach,Malte C.; Carvalho,Marcelo R. de; Nihei,Silvio S..
Saving our science from ourselves: the plight of biological classification. Biological classification ( nomenclature, taxonomy, and systematics) is being sold short. The desire for new technologies, faster and cheaper taxonomic descriptions, identifications, and revisions is symptomatic of a lack of appreciation and understanding of classification. The problem of gadget-driven science, a lack of best practice and the inability to accept classification as a descriptive and empirical science are discussed. The worst cases scenario is a future in which classifications are purely artificial and uninformative.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Classification; DNA barcoding; Morphology; Nomenclature; Systematic; Taxonomy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000200001
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External morphology of immature stages of Zaretis strigosus (Gmelin) and Siderone galanthis catarina Dottax and Pierre comb. nov., with taxonomic notes on Siderone (Lepidoptera: Nymphalidae: Charaxinae) Rev. Bras. entomol.
Dias,Fernando Maia Silva; Oliveira-Neto,José Francisco de; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
ABSTRACT The external morphology of immature stages of Zaretis strigosus (Gmelin, [1790]) and Siderone galanthis catarina Dottax and Pierre, 2009 comb. nov. from southern Brazil are described. Additionally, morphology of the adults and sequences of the mitochondrial gene cytochrome oxidase, subunit I, were analyzed in order to evaluate the taxonomy of Siderone galanthis Hübner, [1823]. Immatures were collected on Casearia sylvestris (Salicaceae) in Curitiba, Paraná, and Balneário Barra do Sul, Santa Catarina, Brazil, and reared at the laboratory. Morphological descriptions and illustrations are provided, based on observations through stereoscopic and optic microscopes attached to camera lucida; results are compared and discussed and immature stages of some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaeini; Chaetotaxy; DNA barcoding; Leafwing; Zaretidinae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000400307
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Phyllocnistis hemera sp. nov. (Lepidoptera: Gracillariidae): a new species of leaf-miner associated with Daphnopsis fasciculata (Thymelaeaceae) in the Atlantic Forest Rev. Bras. entomol.
Fochezato,Júlia; Brito,Rosângela; Isaias,Rosy Mary dos Santos; Gonçalves,Gislene Lopes; Moreira,Gilson R.P..
ABSTRACT During recent studies performed in the Atlantic Forest, a new species of Phyllocnistinae (Gracillariidae), Phyllocnistis hemera sp. nov., leaf miner of Daphnopsis fasciculata (Thymelaeaceae) was discovered. The adults are described and illustrated as well as the immature stages, with notes on natural history including a description of the leaf mine. Additionally, DNA barcode sequences were compared to other representatives of Phyllocnistinae to test for the specific status of P. hemera and to infer phylogenetic relationships. This is the fifth species described for the genus Phyllocnistis in the Atlantic Forest and the first record of a gracillarid mining Thymelaeaceae leaves.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microlepidoptera; New species; Morphology; DNA barcoding; Neotropical region.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100057
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DNA barcoding: a molecular appliance for fish species identification support Sci. Agrar.Paran. / SAP
Tresbach, Rafael Hencke; Cerqueira, Natalia Menezes; Medeiros, Sarah Ramos; Gutierrez, Harold Julián Pérez; Hernández, Natalia Ossa; Rodrigues, Marília Danyelle Nunes.
The identification of genus and species is made by systematic, by noticing morphological and anatomical characteres which were the phenotype expression. However, changes in one or more gene bases can result in phenotypes similarities between different species, inducing an identification error. Alternatively, molecular appliances based in mithocondrial and ribosomal genes appeared. This study aims to compile information about the molecular identification and present the South American Catfish (Rhamdia quelen) case, for which there is controversy generated by taxonomy identification. The different genes used to identification have their particularities, like insertions and deletions on the case of 16S rRNA and the 18S rRNA. Besides these ribosomal genes,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA barcoding; Peixes; Rhamdia quelen; COI; DNA mitocondrial; DNA ribossomal.
Ano: 2015 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408
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The Tonkin weakfish, Cynoscion similis (Sciaenidae, Perciformes), an endemic species of the Amazonas-Orinoco Plume Acta Amazonica
CAIRES,Rodrigo Antunes; SANTOS,Wagner. C. R. dos; MACHADO,Leonardo; OLIVEIRA,Claudio; CERQUEIRA,Najila N. C. D.; ROTUNDO,Matheus Marcos; OLIVEIRA,Cintia; MARCENIUK,Alexandre P..
ABSTRACT Despite its importance in biogeographical, ecological, and commercial terms, the fish fauna of the northern Brazilian coast is still poorly known, representing the least sampled portion of the Brazilian Exclusive Economic Zone. We collected Tonkin weakfish, Cynoscion similis specimens during extensive surveys of the northern Brazilian coast and concluded that C. similis is common in this region. While the species had not previously been reported for the northern Brazilian state of Pará, it may have been recorded in studies of industrial fisheries, being identified only as Cynoscion sp. or by the common name pescada negra. This reinforces the need for the reliable taxonomical identification of species, to guarantee the collection of accurate data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian north coast; Cynoscion jamaicensis; Industrial fisheries; DNA barcoding; Reliable taxonomical identification.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000300197
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Pharmacognostical study of Desmodium caudatum Anais da ABC (AABC)
LI,JUNNI; LIN,XIAOJING; TANG,GENGQIU; LI,RONG; WANG,DONG; JI,SHENGGUO.
Abstract: Desmodium caudatum (Thunb.) DC, is an ever-green plant widely used in the central and southern China with great economic value for their medical values on fever, dysentery, gastroenteritis, rectal prolapse, snake bites, mastitis, and boils carbuncle. Despite its extensive uses as a traditional Chinese medicine, no systematic research on the identification of Desmodium caudatum has been reported. In this study, traditional pharmacognostical identification including the botanical origin and morphological characters, medicinal material characters, microscopic characters, physicochemical parameters determination and phytochemical screening, and DNA barcoding analysis were employed to establish an accurate and effective identification...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desmodium caudatum; DNA barcoding; Quality standardization; Traditional pharmacognostical identification.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300709
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Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits Ciência Rural
Barbosa,Marcílio Souza; Barbosa,Suzanne Nunes; Nagamoto,Nilson Satoru; Forti,Luiz Carlos; lmeida,Cícero.
ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ITS; DNA barcoding; Leaf-cutter; Ants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500150
Registros recuperados: 30
Primeira ... 12 ... Última
 

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