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| Registros recuperados: 31 | |
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| Martins,M.L.; Ghiraldelli,L.; Garcia,F.; Onaka,E.M.; Fujimoto,R.Y.. |
| This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental infection; Nematoda; Camallanus; Crustacea; Notodiaptomus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200018 |
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| Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015 |
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| Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.. |
| The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004 |
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| Chaves,Guilherme M.; Cavalcanti,Maria Auxiliadora de Q.; Carneiro-Leão,Ana Maria dos A.; Lopes,Silvia L.. |
| With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL-1) activity. The other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase (Pz=0.482) and proteinase (0.780 U.mL-1) activity. The strains were inoculated via intraperitoneum in immunosuppressed and non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and kidneys were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Extracellular enzymes; Virulence; Experimental infection. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300010 |
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| Barbosa,Claudio Henrique Gonçalves; Sant'Ana,Fabiano José Ferreira de; Cargnelutti,Juliana Felipetto; Flores,Eduardo Furtado; Teixeira Neto,Antônio Raphael; Santana,Rosália Bezerra de; Reis Junior,Janildo Ludolf. |
| ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: VACV; Experimental infection; Horses; Bovine vaccinia. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519 |
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| Arantes,Tiago Pereira; Lopes,Welber Daniel Zanetti; Ferreira,Roberta Machado; Pieroni,Juliana de Souza Pinto; Pinto,Vanessa Marigo Rocha; Santos,Thaís Rabelo dos; Sakamoto,Claúdio Alessandro; Costa,Alvimar José da. |
| The present research aimed to describe possible histopathological alterations in the reproductive system (testicles and epididymis) of male dogs experimentally infected with Toxoplasma gondii. Canines (n=10) serologically negative for T. gondii were selected and distributed into three experimental groups: GI, 3 inoculated with 2.0 x 10(5)P strain oocysts; GII, 3 infected with 1.0 x 10(6)RH strain tachyzoites; and GIII, 4 control dogs. Antibody research (IFAT) against T. gondii was realized. Toxoplasma gondii infection was confirmed by seroconversion of the 6 males infected with tachyzoites and oocysts from postinoculation day (PID) 7 and 14, respectively. At PID 70, all dogs were submitted to orchiectomy and testicle and epididymis samples were collected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine; Experimental infection; Toxoplasmosis; Histopathology; Testicle; Epididymis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700026 |
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| Registros recuperados: 31 | |
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