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Structure et connectivité de la mégafaune marine à l’échelle d’une région océanique. Enjeux pour la gestion durable des tortues vertes dans l’océan Indien occidental ArchiMer
Bourjea, Jerome.
This thesis is a comprehensive work aiming to improve scientific knowledge on the green turtle (Chelonia mydas) in order to provide key scientific evidences needed for the implementation of coherent and effective management measures to protect at the Western Indian Ocean scale this threatened species. In a first step, this work aimed to established baseline data on the abundance of green turtles nesting females and long term trends of some key nesting populations of the region by applying different modelling methods. In a second step, this work determined the regional genetic structure of this species and the relationships that exists between the different populations. Finally, the conservation of marine turtles being closely dependant to external...
Tipo: Text Palavras-chave: Tortues marines; Tortue verte; Chelonia mydas; Mégafaune; Évaluation de population; Tendance sur long terme; Structure génétique; Génétique des populations; Capture accidentelle; Biologie de la conservation; Unité de gestion; Océan Indien; Marines turtles; Green turtle; Chelonia mydas; Megafauna; Population assessment; Long term trend; Genetic structure; Population genetic; By-catch; Incidental catch; Biological conservation; Management units; Indian ocean.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00244/35505/34009.pdf
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Apport des informations moléculaires et cellulaires pour la caractérisation de la résistance de l'huître plate européenne vis-à-vis de la bonamiose, et pour la détection de signatures de la sélection naturelle ArchiMer
Harrang, Estelle.
The European flat oyster, an endemic species from European coasts, has been classified in the category of "endangered and/or declining species" since 2003. Indeed, the natural beds of this oyster, consumed since ancient times, have gradually been decimated by over-exploitation and by successive emergence of parasitic diseases. The parasite that causes the disease called bonamiosis has contributed to drastically reduce the French and European aquacultural production of flat oyster. Marine bivalve molluscs display two specificities that restrict possibilities to fight against diseases: they are grown in an open environment, and possess an innate immune system lacking in adaptive response. In this context, the selection of animaIs naturally resistant to...
Tipo: Text Palavras-chave: Huître plate Européenne; Ostre edulis; Bonamiose; Bonamia ostreae; Marqueurs SNP; Expression génique; Activité hémocytaire; Cartographie de liaison; QTL; EQTL; Populations naturelles; Diversité génétique; Sélection naturelle; European flat oyster; Ostrea edulis; Bonamiosis; Bonamia ostreae; SNP markers; Gene expression; Haemocytic activity; Linkage map; QTL; EQTL; Natural populations; Genetic diversity; Genetic structure; Natural selection.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00127/23785/21702.pdf
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Population structure of the common sole (Solea solea) in the Northeastern Atlantic and the Mediterranean Sea: revisiting the divide with EPIC markers ArchiMer
Rolland, Jean-luc; Bonhomme, Francois; Lagardere, Francoise; Hassan, M; Guinand, Bruno.
Spatial and temporal population genetic structures of the common sole, Solea solea, were studied in Northeastern Atlantic and Mediterranean Sea populations, using three polymorphic exon-primed intron-crossing (EPIC) markers. Results demonstrated significant multilocus differentiation among Eastern Mediterranean and a group composed by Western Mediterranean and Atlantic populations (theta = 0.150, P < 0.001), but also suggested unrecorded genetic differentiation of the Adriatic Sea population. No pattern of isolation-by-distance was recorded across the range covered by sampling, from the Kattegat to the Aegean Sea. Conversely to genetically structured Mediterranean populations, Atlantic populations ranging from Denmark to Portugal could be considered as...
Tipo: Text Palavras-chave: Marine fish; Genetic structure; Introns; Common sole.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2400.pdf
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Detailed insights into pan-European population structure and inbreeding in wild and hatchery Pacific oysters (Crassostrea gigas) revealed by genome-wide SNP data ArchiMer
Vendrami, David L. J.; Houston, Ross D.; Gharbi, Karim; Telesca, Luca; Gutierrez, Alejandro P.; Gurney-smith, Helen; Hasegawa, Natsuki; Boudry, Pierre; Hoffman, Joseph I..
Cultivated bivalves are important not only because of their economic value, but also due to their impacts on natural ecosystems. The Pacific oyster (Crassostrea gigas) is the world's most heavily cultivated shellfish species and has been introduced to all continents except Antarctica for aquaculture. We therefore used a medium-density single nucleotide polymorphism (SNP) array to investigate the genetic structure of this species in Europe, where it was introduced during the 1960s and has since become a prolific invader of coastal ecosystems across the continent. We analyzed 21,499 polymorphic SNPs in 232 individuals from 23 localities spanning a latitudinal cline from Portugal to Norway and including the source populations of Japan and Canada. We confirmed...
Tipo: Text Palavras-chave: Aquaculture; Crassostrea gigas; Genetic structure; High-density genotyping array; Inbreeding; Pacific oyster; Restriction site-associated DNA (RAD) sequencing; Single nucleotide polymorphism (SNP).
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00484/59561/62557.pdf
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Relationships between sediments and tissue contamination and allozymic patterns in Hediste diversicolor (Polychaeta Nereididae) in the Pialassa lagoons (north Adriatic Sea) ArchiMer
Virgilio, M; Baroncini, N; Trombini, C; Abbiati, M.
An increasing number of investigations revealed that responses of populations to environmental stressors may be genetically dependent. Occurrence of genetic adaptation to high concentrations of heavy metals in Hediste diversicolor local ecotypes has also been hypothesised. Analyses of allozymic patterns are a promising tool for the genetic monitoring of environmental stressors. The aim of this study was to investigate whether the allozymic patterns of H. diversicolor could be affected by levels of contamination. Five sites were investigated: four in a polluted brackish habitat, the Pialassa lagoons, and a reference site in the Lamone estuary. Total mercury was used as a tracer of the overall level of contamination. Concentrations of mercury were quantified...
Tipo: Text Palavras-chave: Hediste diversicolor; Contamination; Structure génétique; Adaptation; Systèmes enzymatiques; Hediste diversicolor; Contamination; Genetic structure; Adaptation; Allozyme.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43288/43023.pdf
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Genetic structure and demographic history of striped weakfish Cynoscion guatucupa (sciaenidae) from the southwestern atlantic JBAG
Alonso,M.P; Fernández Iriarte,P.J.
In South America, the Pleistocene was characterized by important environmental changes related to glacial cycles, which had significant effects on the evolutionary history of several marine species. To evaluate the pattern of demographic history and the genetic structure of striped weakfish Cynoscion guatucupa, a 401 bp fragment of the mitochondrial Cytochrome b gene was sequenced from 92 individuals from three coastal areas in Argentina and one in Brazil in the southwestern Atlantic. Haplotype diversity was high, whereas nucleotide diversity was low among all sampling sites. The star-like pattern was not phylogeographically structured, in agreement with the AMOVA analysis. The Fu&#8217;s test was negative and highly significant whereas the mismatch...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Cytochrome b; Marine fish; Pleistocene; Mutation rate.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300001
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Genetic variability of Five Periplaneta americana L. (Dyctioptera: Blattidae) populations in southwestern Colombia using the AFLP molecular marker technique Neotropical Entomology
Jaramillo-Ramirez,Gloria I; Cárdenas-Henao,Heiber; González-Obando,Ranulfo; Rosero-Galindo,Carol Y.
The genetic structure of five Periplaneta americana (L.) populations from three cities (Cali, Popayán and Buenaventura) located in southwestern Colombia was estimated using the AFLP molecular marker technique. A set of 174 loci were analyzed, 120 of which were polymorphic. Genetic diversity was estimated at different geographic levels for the total population and between cities. Genetic diversity averaged 0.32. The largest gene flow was detected between two population from Cali (F ST = 0.088; Nm = 2.6) and the lowest from the third population in this city (Univalle) and Popayán and Buenaventura (F ST = 0.13; Nm = 1.6 and F ST = 0.12; Nm = 1.8, respectively). The individuals' low mobility, the population' geographic separation, and possibly genetic drift...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Population differentiation; Urban pest.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000300010
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Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis Neotropical Entomology
Sosa-Gomez,Daniel R.; Delpin,Katiaíres E.; Almeida,Alvaro M.R.; Hirose,Edson.
The Neotropical Brown Stink Bug (NBSB), Euschistus heros (Fabricius), has a wide distribution in Brazilian soybean fields, being more important in the central region of the country. The species is the main target of insecticide applications for stink bug pest control. We determined the variability among and within populations of the NBSB by random amplification of polymorphic DNA (RAPD) analysis. Samples of NBSB were collected in soybean fields from Ubiratã (PR), Londrina (PR), Centenário do Sul (PR), Cândido Mota (SP), Ponta Porã (MS) and Sapezal (MT). Genomic DNA was extracted from the head to minimize DNA contamination by endoparasites. DNA was amplified with 10 mer primers. Fifteen primers produced 246 bands. The genetic similarity matrix was obtained,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Molecular marker; Genetic structure; Gene flow.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200009
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Population genetics of three threatened catfish species in heterogeneous environments of the Cauca River, Colombia Neotropical Ichthyology
Restrepo-Escobar,Natalia; Yepes-Acevedo,Anny Johanna; Márquez,Edna Judith.
ABSTRACT Neotropical catfishes Ageneiosus pardalis, Pimelodus grosskopfii and Sorubim cuspicaudus are migratory fishes of commercial importance that exhibit decreasing populations due to overfishing and other anthropic interventions. This study used species-specific microsatellite loci to test the hypothesis that threatened fish populations show genetic vulnerability signs and are genetically structured in the middle and lower sections of the Cauca River. The studied species exhibit genetic diversity levels higher than the average values reported for Neotropical Siluriformes; however, they seem to have suffered recent bottlenecks and they present significant endogamy levels that are higher for the critically endangered catfish P. grosskopfii. Furthermore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fish; Genetic diversity; Genetic structure; Microsatellites; Siluriformes.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100204
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Genetic diversity of the species Leporinus elongatus (Teleostei: Characiformes) in the Canoas Complex - Paranapanema River Neotropical Ichthyology
Ramos,Juliano Vilas Boas; Sodré,Leda Maria Koelblinger; Orsi,Mário Luís; Almeida,Fernanda Simões de.
Dams constructed along waterways interrupt the dispersion and migration of aquatic organisms, affecting mainly the abundance of migratory fish species. Translocation mechanisms have been constructed at dams aiming to minimize their impact on fish species migration behavior. There is little information available about the effect of the construction of dams on the genetic structure of the Neotropical migratory fish fauna. Therefore, RAPD molecular markers and microsatellites were utilized to evaluate the diversity and genetic structure of the migratory species Leporinus elongatus (piapara) in the Canoas Complex - Paranapanema River - Brazil. Ten groups were sampled in the fish ladders of the hydroelectric dam Canoas I and Canoas II during the reproductive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dams; Fish ladder; Genetic structure; Microsatellite; RAPD.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000400015
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Phylogeographic pattern of Jenynsia multidentata(Cyprinodontiformes: Anablepidae) in the southern boundary of the Brazilian Subregion, Argentina Neotropical Ichthyology
Bruno,María Cecilia; Casciotta,Jorge Rafael; Almirón,Adriana Edith; Lizarralde,Marta Susana.
The Atlantic drainage basins are located in the southern Pampean area, in the southernmost part of the Brazilian subregion. Tectonic and paleoclimatic phenomena, occurring during the Quaternary, have currently isolated these basins from the present hydrographic system. Their ichthyofaunal composition is similar to that of hydric systems located further northward. Jenynsia multidentata is a species with wide distribution in the Atlantic drainage basins, as well as in those Northern basins. Here we investigated the phylogeographic pattern of Jenynsia multidentataand analyzed its relationships with the paleoclimatic and geologic history of the region. The analysis of the population's assemblage showed moderate genetic diversity, demographic equilibrium and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Control region; Genetic structure; Ichthyofauna; Phylogeography; Southern Pampean area.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300477
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Genetic diversity of cultivated accessions and wild species of rubber tree using EST‑SSR markers PAB
Perseguini,Juliana Morini Küpper Cardoso; Romão,Lineu Roberto de Castro; Briñez,Boris; Scaloppi Junior,Erivaldo José; Gonçalves,Paulo de Souza; Benchimol,Luciana Lasry.
The objective of this work was to evaluate the efficiency of EST‑SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty‑five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST‑SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST‑SSRs showed from two to ten alleles and were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerprinting analysis; Genetic structure; Functional molecular markers; Polymorphism information content; Transferability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800008
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Conservação genética de populações de Cryptocarya moschata Nees (Lauraceae) na Mata Atlântica do estado de São Paulo Rev. Bras. Bot.
MORAES,PEDRO LUÍS RODRIGUES DE; MONTEIRO,REINALDO; VENCOVSKY,ROLAND.
Através da análise de 18 locos isoenzimáticos polimórficos, foram estimadas as freqüências alélicas referentes a 214 indivíduos adultos de quatro populações naturais de Cryptocarya moschata de duas regiões do estado de São Paulo. Com base nas heterozigosidades observadas e esperadas, foram obtidas estimativas das estatísticas F de Wright. Para fins de comparação, utilizou-se também o método da análise da variância para estimação dos parâmetros correspondentes F = FIT, <FONT FACE="Symbol">q</font>P= FST e f = FIS. Os dois métodos forneceram resultados bastante concordantes: <img src="http:/img/fbpe/rbb/v22s2/f.gif" alt="f.gif (76 bytes)">IT = 0,142; <img src="http:/img/fbpe/rbb/v22s2/f.gif" alt="f.gif (76 bytes)">ST = 0,140; <img...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic structure; Effective population size; Gene flow; Cryptocarya moschata.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000500004
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A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piauí, Brazil R. Bras. Zootec.
Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros.
The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155
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Allozymic variation in the clam genus Eurhomalea (Bivalvia: Veneriidae) along southern South American coast RChHN
GALLARDO,M.H; GONZÁLEZ,C; MENA,C; LOMOVASKY,B; MORRICONI,E; CLASING,E.
The correspondence between allozymic variation and specific differentiation was studied in four populations corresponding to the three nominal, allopatric species of clam genus Eurhomalea (E. rufa, E. lenticularis, E. exalbida) described for southern South America. Allozyme variation scored in 12 loci was high as indicated by heterozygosity levels (15.8-20.7 %) and by the presence of only three monomorphic loci (Hk-2, Icd-2, and Xdh-1). These high estimates of allelic variability were influenced by the low levels of interspecific genetic similarity (I = 0.64) and for the high conspecific values of genetic identity observed. The high estimates of substructuring found at the species level (F ST = 0.39) contrasted with the low differentiation (F ST = 0.027)...
Tipo: Journal article Palavras-chave: Allozymic variation; Genetic structure; Taxonomic status.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2003000300013
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Comparative genetic structure in pines: evolutionary and conservation consequences RChHN
DELGADO,PATRICIA; CUENCA,ARGELIA; ESCALANTE,ANA E.; MOLINA-FREANER,FRANCISCO; PIÑERO,DANIEL.
Pines have been the focus of several studies that estimate population genetic parameters using both allozymes and chloroplast single sequence repeats (SSRs). Also, the genus has also been recently studied using molecular systematics so that we now have a more clear understanding of their evolutionary history. With this background we studied comparatively the genetic structure in pines. Expected heterozygosity is particularly constant with a 99 % confidence interval between 0.19 and 0.23 in species that have been studied until now using allozymes. There is a significant proportion of species (9/41) that show high population differentiation estimates (Fst = or larger than 0.15) and five of these have large and wingless seeds probably associated with low...
Tipo: Journal article Palavras-chave: Pinus; Genetic structure; Outcrossing rates; Microsatellites; Allozymes.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2002000100004
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A lima bean core collection based on molecular markers Scientia Agricola
Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin.
ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100
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Genetic diversity of American wild rice species Scientia Agricola
Veasey,Elizabeth Ann; Bressan,Eduardo de Andrade; Zucchi,Maria Imaculada; Vencovsky,Roland; Cardim,Daruska Cavalcante; Silva,Rainério Meireles da.
Studies on genetic diversity and genetic structure of natural populations are important in order to define strategies for in situ and ex situ conservation actions and for plant pre-breeding programs. Aiming to assess the genetic diversity and genetic structure of three wild American Oryza species with isozyme markers, 14 populations of the diploid O. glumaepatula (AglAgl), 11 populations of the tetraploid O. grandiglumis (CCDD) and five populations of the also tetraploid O. latifolia (CCDD) were studied. They were all originated from Rio Paraguay hydrographic basin and the Amazon. Four enzymes were used and they gave 40 polymorphic bands. The most polymorphic species was O. glumaepatula, followed by O. latifolia and O. grandiglumis. A cluster analysis with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza glumaepatula; O. latifolia; O. grandiglumis; Amazon; Genetic structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400008
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The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) Biol. Res.
Moresco,Rafaela M; Maniglia,Thiago C; De Oliveira,Classius; Margarido,Vladimir P.
The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the...
Tipo: Journal article Palavras-chave: ESU; Gene flow; Genetic structure; Molecular marker.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100008
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Genetic structure of natural populations of Cryptocarya moschata Nees (Lauraceae) from southeastern Brazilian Atlantic rain forest Biota Neotropica
Moraes,Pedro Luís Rodrigues de; Derbyshire,Maria Teresa Vitral de Carvalho.
This study was accomplished on a more comprehensive basis to evaluate previous questions that were raised from a preliminary article about the genetic structure of Cryptocarya moschata populations. Thus, through the analysis of 40 polymorphic allozyme loci, allele frequencies were estimated from 335 individuals of 11 natural populations of C. moschata from six hydrographic basins of São Paulo state and Serra da Estrela, Rio de Janeiro, Brazil. Estimates of Wright's F statistics were done through the analysis of variance, presenting average values of <img border=0 width=32 height=32 id="_x0000_i1026" src="../../../../../../img/revistas/bn/v4n1/img/a04car(f).jpg" align=absmiddle > or = 0.352, <img border=0 width=32 height=32 id="_x0000_i1027"...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Lauraceae; Genetic structure; Neotropics; Cryptocarya moschata; Atlantic rain forest; Brazil.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032004000100004
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