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| Registros recuperados: 158 | |
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| Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira. |
| Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Genetic variability; Bacterium; Citrus; PFGE. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020 |
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| Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo. |
| Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011 |
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| Lassala,Maria Dolores Peres; Renesto,Erasmo. |
| We estimated the genetic variability of nine fish species from the Brazilian upper Paraná River floodplain (Astyanax altiparanae, Hoplias malabaricus, Leporinus lacustris, Loricariichthys platymetopon, Parauchenipterus galeatus, Pimelodus maculatus, Rhaphiodon vulpinus, Roeboides paranensis and Serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (EC 2.6.1.1), acid phosphatase (EC 3.1.3.2), esterase (EC 3.1.1.1), glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose-6-phosphate isomerase (EC 5.3.1.9), Iditol dehydrogenase (EC 1.1.1.14), isocitrate dehydrogenase - NADP+ (EC 1.1.1.42), L-lactate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Heterozygosity; Paraná River; Polymorphism; Reproductive strategies; Tropical fish. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400030 |
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| Dorji,Nedup; Duangjinda,Monchai; Phasuk,Yupin. |
| The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowllike, corresponding to Yuebjha Narp, Phulom, Khuilay and Seim, respectively), two subspecies of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), two varieties of Thai native chickens (Pradhu Hang Dam and Chee; Gallus gallus domesticus) representing the Southeast Asian domestic chicken, and two commercial lines (Broiler and Single Comb White Leghorn) were genotyped with 18 microsatellites that included 16... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Genetic comparisons; Genetic variability; Microsatellites. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400010 |
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| Moreno,Vanessa Roma; Grisolia,Alexéia Barufatti; Campagnari,Francine; Milazzotto,Marcela; Adania,Cristina Harumi; Garcia,José Fernando; Souza,Edislane Barreiros de. |
| We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Mammals; Felines; Microsatellites. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200017 |
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| Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi. |
| Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006 |
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| Rizza,Marco Dalla; Real,Daniel; Reyno,Rafael; Porro,Valentina; Burgueño,Juan; Errico,Eugenia; Quesenberry,Kenneth H.. |
| Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA content; Genetic variability; Inter-SSR; Trifolium spp. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600015 |
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| Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol. |
| A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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| Bessega,Cecilia; Saidman,Beatriz Ofelia; Vilardi,Juan César. |
| In the present work, isoenzyme electrophoresis was used to analyze the variability and phenetic relationships among seven American species of genus Prosopis belonging to three different sections: P. argentina (Monilicarpa), P. glandulosa, P. velutina, P. flexuosa, P. ruscifolia, P. kuntzei (Algarobia), and P. reptans (Strombocarpa). The genetic variability in P. argentina, P. reptans, and P. kuntzei was significantly lower than in the rest of the species analyzed. The species belonging to different sections are highly differentiated, but the relationships retrieved among species belonging to the section Algarobia suggested that the series of this section are not natural groups. P. kuntzei is as differentiated from the remaining species of Algarobia as from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prosopis; Isoenzymes; Genetic variability; Phenetic relationships. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200018 |
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| Fazzi-Gomes,Paola; Guerreiro,Sávio; Palheta,Glauber David Almeida; Melo,Nuno Filipe Alves Correa de; Santos,Sidney; Hamoy,Igor. |
| Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tambaqui; Genetic variability; Gene flow; Genetic structure; Single sequence repeats. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100142 |
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| Boraschi,Daniele; Del Lama,Marco Antonio. |
| Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013 |
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| Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland. |
| Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016 |
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| Registros recuperados: 158 | |
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