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| Registros recuperados: 36 | |
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| Astuya, Allisson; Carrera, Crisleri; Ulloa, Viviana; Aballay, Ambbar; Nunez-acuna, Gustavo; Hegaret, Helene; Gallardo-escarate, Cristian. |
| Saxitoxin (STX) is a neurotoxin produced by dinoflagellates in diverse species, such as Alexandrium spp., and it causes paralytic shellfish poisoning (PSP) in humans after the ingestion of contaminated shellfish. Recent studies have suggested that the immune functions of bivalves could be affected by harmful algae and/or by their toxins. Herein, hemocytes are the main effector cells of the immune cellular response. In this study, we evaluated the response of hemocytes from the mussel Mytilus chilensis to STX exposure in a primary culture. Cell cultures were characterized according to size and complexity, while reactive oxygen species (ROS) production was evaluated using a dichlorofluorescein diacetate (DCFH-DA) assay. Finally, phagocytic activity was... |
| Tipo: Text |
Palavras-chave: Saxitoxin; Hemocytes; Immune response; Paralytic shellfish poisoning; Reactive oxygen species (ROS). |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00278/38954/73180.pdf |
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| Roberts, S; Gueguen, Yannick; De Lorgeril, Julien; Goetz, F. |
| Interleukin-17 (IL-17) gene models have been found in the sequenced genomes of Strongylocentrotus purpuratus and Caenorhabditis elegans. However, there have been no published reports on the empirical cloning and characterization of any interleukin cDNAs in invertebrates. From a Pacific oyster (Crossostrea gigas) hemocyte cDNA library, two clones were obtained that encoded a protein similar to vertebrate IL-17s. The putative oyster IL-17 homolog (CgIL-17) was 27% identical to rainbow trout IL-17D, 21% to human IL-17D and 24% to an IL-17D-like gene model obtained from the annotation of the sea urchin genome. IL-17s from the oyster, sea urchin, trout and human, contained conserved cysteine residues found in all forms of IL-17 in mammals. Injection of bacteria... |
| Tipo: Text |
Palavras-chave: Invertebrate innate immunity; Crassostrea gigas; Invertebrate cytokines; Hemocytes; Pacific oyster; Interleukin. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4151.pdf |
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| Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan. |
| Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster... |
| Tipo: Text |
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf |
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| Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane. |
| The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval... |
| Tipo: Text |
Palavras-chave: Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2612.pdf |
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| Benabdelmouna, Abdellah; Saunier, Alice; Ledu, Christophe; Travers, Marie-agnes; Morga, Benjamin. |
| In the context of the abnormal mass mortality of mussels in France since 2014, Flow CytoMetry (FCM) was used in 2015 and 2016 to study the DNA content and cell cycle characteristics of hemic circulating cells collected from 2000 mussels. The mussels were sampled from 12 wild and cultivated blue mussels stocks distributed along the French Atlantic coast from the south Brittany to Pertuis Charentais areas. During these surveys, various genetic abnormalities were frequently detected, and ploidy characteristics revealed contrasting profiles that corresponded to respective contrasting sanitary status, i.e. healthy mussels with high cytogenetic quality (HCQ) versus diseased mussels with low cytogenetic quality (LCQ). In the present work, FCM and hemocytology... |
| Tipo: Text |
Palavras-chave: Mussels; Hemocytes; Flow cytometry; Hemocytology; Cytogenetic quality; Neoplasia. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56623/58443.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf |
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| Carneiro,Maria Elisa; Daemon,Erik. |
| With the purpose of characterize morphologically the hemocytes of larvae and nymphs of Rhipicephalus sanguineus on different nutritional phases, it was obtained samples of haemolymph were obtained by sectioning the forelegs and collecting the drop there formed. After dried, the samples were fixed by methanol and stained by Giemsa. Microscopical observation resulted in the characterization of five basic cellular types: prohemocytes, plasmatocytes, granulocytes, spherulocytes and oenocytoids. Moreover, undefined cell types, whose morphological patterns didn't have correlation with the characteristics cited for hemocytes, were found in low frequency. The change in the relative composition of the haemolimph was characterized by decrease of the number of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ixodid; Rhipicephalus sanguineus; Hemocytes; Morphology. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751996000300010 |
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| GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C.. |
| This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973 |
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| PASSOS,ELIANA M. DOS; WANDERLEY-TEIXEIRA,VALÉRIA; MARQUES,EDMILSON J.; TEIXEIRA,ÁLVARO A.C.; BRAYNER,FÁBIO A.. |
| The present research aimed to elucidate which aspects of immune responses in Diatraea flavipennella are suppressed by the parasitoid Cotesia flavipes, thus, ensuring parasitism success. We investigated the presence of apoptosis in fat body cells through the TUNEL technique. According to the results, reduced levels of nitric oxide and phenoloxidase activity were observed in larvae parasitized for three days, and reduced total number of hemocytes, after three and seven days. An increase in plasmatocytes and decrease in spherulocytes numbers were observed in the differential count on the third day of parasitism. The number of melanized microspheres in parasitized larvae was low and indicated less intense melanization. The ultrastructural analysis confirmed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Encapsulation; Hemocytes; Humoral response; Parasitoid; Sugarcane Moth Borer (Diatraea saccharalis). |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402013 |
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| Falleiros,Ângela Maria Ferreira; Bombonato,Maria Terezinha Siqueira; Gregório,Elisa Aparecida. |
| Six circulating hemocytes cell types from Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) larvae were identified by transmission and scanning electron microscope: prohemocytes (PR), plasmatocytes (PL) granulocytes (GR), spherulocytes (SP), oenocytoids (OE) and vermicytes (VE). The PR was the smallest cell type with a large nucleus, a cytoplasm with few organelles and a homogenous smooth surface. The PL was polymorphic and abundant, with a cytoplasm rich in organelles and a cellular surface with several cytoplasmic projections. The GR was abundant, showing two types of membrane-bounded granules (dense and structutered), glycogen, lipid droplets and a surface with philopodial projections. The SP was a large cell, with a cytoplasm full of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insect; Lepidoptera; Hemocytes; Ultrastructural Morphology; Differential Hemocyte Count; Morphometry. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200021 |
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| Registros recuperados: 36 | |
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