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| Registros recuperados: 47 | |
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| Decker, Carole; Zorn, N.; Le Bruchec, J.; Caprais, Jean-claude; Potier, N.; Leize-wagner, E.; Lallier, F. H.; Olu, Karine; Andersen, A. C.. |
| Vesicomyids live in endosymbiosis with sulfur-oxidizing bacteria and therefore need hydrogen sulfide to survive. They can nevertheless live in a wide range of sulfide and oxygen levels and depths, which may explain the exceptional diversity of this clam family in deep-sea habitats. In the Gulf of Guinea, nine species of vesicomyid clams are known to live in cold-seep areas with pockmarks from 600 to 3200 m deep, as well as in the organic-rich sediments of the Congo deep-sea fan at 5000 m deep. Our previous study showed that two species living in a giant pockmark have different oxygen carriers, suggesting different adaptations to hypoxia. Here, we studied the hemoglobin structure and oxygen affinity in three other species, Calyptogena valdiviae, Elenaconcha... |
| Tipo: Text |
Palavras-chave: Hemoglobin; Mass spectrometry; Oxygen affinity; Symbiont-bearing bivalve; Blood-clams; Cold seeps; Sulfide-rich sediments. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00358/46961/46875.pdf |
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| Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia. |
| Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own... |
| Tipo: Text |
Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf |
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| Marcaillou-le Baut, Claire; Haure, Joel; Mondeguer, Florence; Courcoux, Anne; Dupuy, Beatrice; Penisson, Christian. |
| The objective of this study was to identify a possible effect of food on the acceleration of decontamination in blue mussels, Mytilus edulis, containing diarrhetic toxins belonging to the okadaic acid (OA) structural group. An experimental protocol was designed to describe and compare the decontamination kinetics and detoxification rates of naturally OA-contaminated mussels that had either received or not received food for three weeks. The protocol was applied in two trials (in June 2006 and June 2007, called Ker06 and Ker07), conducted one year apart on samples of mussels collected in the same area, at the same season. Okadaic acid (OA), the main lipophilic toxin produced by the toxic alga Dinophysis acuminata, was analysed over the course of the... |
| Tipo: Text |
Palavras-chave: Mussel; Feeding; Digestive gland; Depuration; Okadaic acid; Acyl esters; Marine lipophilic biotoxins; Mass spectrometry. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00025/13650/10883.pdf |
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| Juin, Camille; Bonnet, Antoine; Nicolau, Elodie; Berard, Jean-baptiste; Devillers, Romain; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent. |
| A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns... |
| Tipo: Text |
Palavras-chave: Carotenoid; Chlorophyll; Dereplication; Divinyl chlorophyll a; Galactosyldiacylglycerol; Gracilamide; Mass spectrometry; MSE; Phytoplankton; Pigment; Porphyridium purpureum; UPLC. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/37994/36069.pdf |
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| Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire. |
| Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf |
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| Conceição,K; Bruni,FM; Sciani,JM; Konno,K; Melo,RL; Antoniazzi,MM; Jared,C; Lopes-Ferreira,M; Pimenta,DC. |
| Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrine-type serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was pre-fractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phyllomedusa nordestina; Bradykinin; Mass spectrometry; Natural peptides. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400004 |
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| Santos,LD; Pieroni,M; Menegasso,ARS; Pinto,JRAS; Palma,MS. |
| Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hymenoptera; Venoms; Allergens; Phospholipase A1; Wasp venom antigen 5; Hyaluronoglucosaminidase; Mass spectrometry; Proteomics. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400003 |
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| Vigerelli,Hugo; Sciani,Juliana Mozer; Jared,Carlos; Antoniazzi,Marta Maria; Caporale,Graciane Maria Medeiros; Silva,Andréa de Cássia Rodrigues da; Pimenta,Daniel C. |
| Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules in nature. They are present in numerous biological fluids, including the skin secretion of many amphibians, in which they act (together with proteins, peptides and steroids) as protection agents against predators and/or microorganisms. Among those amphibians that are rich in alkaloids, there is the genus Rhinella.Methods Bufotenine was isolated from Rhinela... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies; Rhinella; Bufotenine; Alkaloids; Toxins; Mass spectrometry. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200336 |
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| Binh,DV; Thanh,TT; Chi,PV. |
| Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Naja naja; Snake venom proteome; Thermostable peptides; Mass spectrometry; Analgesic activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400014 |
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| MATOS,A.K.A.; CARBONARI,C.A.; VELINI,E.D.; GOMES,G.L.G.C.; TRINDADE,M.L.B.; MACEDO,G.C.. |
| ABSTRACT The objective of this study was to evaluate the availability of herbicides clomazone and tebuthiuron in the solution in different kinds of soils saturated with water or vinasse. Samples of 30 soils with different characteristics were arranged on trays to the herbicides spraying. Then they were homogenized, placed in plastic cartridges and saturated with deionized water or vinasse, and remaining at rest during 18 hours. Two extractions were made, the first one quantified the presence of the herbicides in the soil solution and in the second one the total extraction of herbicide remaining in the soil was taken to determine the recovery percentage of each herbicide tested. For quantification, a LC-MS/MS system was used, a compound of a high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugarcane; Mass spectrometry; HPLC; Sorption. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000400771 |
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| Mendonça,Angélica; Bernardi Marchiotti,Rafaella C.; Firmino,Ellen L.B.; Santos,Pollyanna P.; Sguarizi Antonio,Denise; Serrão,José E.; Cardoso,Claudia A.L.; Antonialli Junior,William F.. |
| ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mass spectrometry; MALDI-TOF/TOF; Proteins; Wasps. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000400322 |
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| Jesus,Monalisa Santana Coelho de; Santiago,Manuela Cristina Pessanha de Araújo; Pacheco,Sidney; Gouvêa,Ana Cristina Miranda Senna; Nascimento,Luzimar da Silva de Mattos do; Borguini,Renata Galhardo; Guerra,José Guilherme Marinho; Espindola,José Antonio Azevedo; Godoy,Ronoel Luiz de Oliveira. |
| ABSTRACT Acylated anthocyanins from a purple-fleshed sweet potato (PFSP), obtained by organic cultivation in Brazil, were characterized after separation by a high performance liquid chromatography-diode array detector (HPLC-PDA). These anthocyanins were manually collected at the detector output, concentrated and injected into a high resolution mass spectrometer (ESI-QTOF-MS2). Twenty-two acylated anthocyanins were detected. Among them, sixteen had been reported in the literature and six, derived from peonidin were reported for the first time in sweet potato roots in this study. These compounds showed molecular ions with accurate mass/charge ratios (m/z) of 909.2081, 961.3010, 961.2571, 963.3345, 1123.2932 and 1179.3862. Although anthocyanins in PFSP have... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenolic; Mass spectrometry; Natural dye; Antioxidant. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000400901 |
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| Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos. |
| The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009 |
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| Registros recuperados: 47 | |
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