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Etude experimentale preliminaire sur les echanges gazeux chez Pinna nobilis ArchiMer
Vicente, N; Riva, A; Butler, A.
Breathing exchanges of Pinna nobilis L. are studied in situ and in the laboratory with two methods. First method consist in introducing samples of Pinna under a 40l plexiglass bell equipped with haps. The bell is positionned by diving. The consumption of oxygen is observed with water samples and the oxygen concentration is measured with an electronic oxymeter. Experiments which are carried out during nycthemeral cycles are reproduced in the laboratory with the same type of bell. A second method carry into effect an apparatus more sophisticated, who put into circulation a current of water in an airtight thermostated cellule, where a specimen of young Pinna is introduced. This sample is gradually adaptated to the conditions of the medium. Breathing...
Tipo: Text Palavras-chave: Pinna nobilis; Bivalvia; Temperature; Metabolism; Oxygen consumption; Experimental research; Breathing mixtures; Marine molluscs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1761.pdf
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Production and consumption of B group vitamins in situ. ArchiMer
Kurata, A.
The changes in concentration of thiamine, biotin and vitamin B sub(12) in water of the soaked bottles for 6 h in situ in the north basin of Lake Biwa were examined successively over 30 hours. The concentrations of the vitamins changed comparatively during 6 h. soaking in situ. Generally, the concentrations of thiamine, biotin and vitamin B sub(12) were in high levels in the morning and in low levels in evening both in the soaked bottles and in the surrounding water. The fluctuation ranges in concentration of vitamins in a 5 m deep layer were greater than those in thermocline. From the data obtained with the soaked bottles in situ, it was supposed that the decrease of vitamins in concentration during the daytime surpass consumption of vitamins by...
Tipo: Text Palavras-chave: Phytoplankton; Microorganisms; Bacteria; Metabolism; Biological production; Vitamin B; Fresh water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-958.pdf
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Etude de la diversité métabolique des micro-organismes des sources hydrothermales ArchiMer
Byrne, Nathalie.
Since the discovery of oceanic hydrothermal vents, microbial diversity of this extreme environment was studied using both cultivation and molecular approaches. In this study the microbial diversity of active chimney from both TAG and Lucky Strike sites (Mid Atlantic Ridge) was investigated using both molecular survey and continuous enrichment culture in a bioreactor. To reproduce as close as possible environmental conditions the cultures were performed in oligotrophic conditions, in the presence of hydrogen and carbondioxide in the gas sweeping mix or using in situ sampled fluid/sea water mix as culture medium. The effect of environmental changes such as temperature and the absence or presence of sulphur was also investigated. Archaeal and Bacterial...
Tipo: Text Palavras-chave: Culture; Molecular diversity; Metabolism; Hydrothermal vents; Microorganisms; Microbiology; Culture; Diversité moléculaire; Métabolisme; Sources hydrothermales; Micro organismes; Microbiologie.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-7295.pdf
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Methods for microbial biomass, community structure and metabolic activities on surfaces. ArchiMer
White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf
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Substitution of fish meal with plant protein sources and energy budget for white shrimp Litopenaeus vannamei (Boone, 1931) ArchiMer
Suarez, J. A.; Gaxiola, G.; Mendoza, R.; Cadavid, S.; Garcia, G.; Alanis, G.; Suarez, A.; Faillace, J.; Cuzon, Gerard.
Fish meal (FM) has long been considered an essential ingredient for the formulation of shrimp feeds. Until recently, 12%FM was considered a minimum amount, below which weight gain would become reduced due to a reduction in feed intake. In order to test this perceived minimum value, an experiment was conducted on a long-term basis with 4 replicates. Four practical isonitrogenous and isoenergetic diets were formulated for Litopenaeus vannamei juveniles. Survival, weight gain, feed conversion ratio (FCR) and protein efficiency ratio were determined for 0, 6, 10 and 15% FM diets and a reference diet. Shrimp survival ranged from 84 to 86.5%. Shrimp fed a diet with 0% FM showed lower average weight and SGR compared to the other treatments (P<0.05). FCR of...
Tipo: Text Palavras-chave: Metabolism; Growth; Plant proteins; Substitution fish meal; L. vannamei.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6630.pdf
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Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection ArchiMer
Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf
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Nutrition phosphoree chez Chondrus crispus (Rhodophycees) ArchiMer
Chopin, T; Floc'H, J; Bodeau, B; Penot, M.
Phosphorated nutrition of the red alga Chondrus crispus Stackh. is studied is two morphologically very dissimilar forms of the thallus. Phosphorus measurements, which were monthly carried out at different parts of the alga, showed first that the distribution of this element is not homogeneous along the thallus, and second that seasonal variations occur. It is suggested that the classical polymorphism of the species can be taken into account as concerns its physiology. Besides, from absorption and exsorption experiments which were performed with super(32)P as a tracer, it is shown that the high concentration factor of phosphorus in the alga as compared to the environmental seawater (up to 17.9 x 10 super(4)) can be explained as an effect of metabolic process.
Tipo: Text Palavras-chave: Chondrus crispus; Algae; Distribution; Metabolism; Seasonal variations; Food absorption; Phosphorus; Plant nutrition.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1175.pdf
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L'adaptation ecophysiologique et sanitaire de l'ecrevisse turque Astacus leptodactylus a des milieux saumatres: Contribution a l'astaciculture ArchiMer
Barbe, D; Lautier, J; Vernet, G; Vey, A.
This influence of salt media on different aspects of biological cycle and on most common pathogenic agents of Turkish freshwater crayfish Astacus leptodactylus is investigated survival and growing of juveniles O super(+) and intermediary individuals 1 super(+) are also studied. Total osmotic metabolism and reproduction, have been recorded. It appears that youngest individuals are particularly sensitive to low salinities (7 ppt.). Intermediary ones are more resistant nevertheless salinity does not improve neither survival nor growing. Copulation, laying and pleopod egg fixation are particularly affected by salt waters. The only promising application of salinity parameter on astaciculture could be whether prophylactic or curative treatment with concentrated...
Tipo: Text Palavras-chave: Astacus leptodactylus; Malacostraca; Infectious diseases; Sexual reproduction; Growth; Mortality; Survival; Osmotic adaptations; Crayfish culture; Pathology; Metabolism; Salinity; Ecophysiology; Astaciculture; Régulation osmotique; Reproduction; Survie; Croissance; Pathologie; Ecophysiologie; Salinité; Astacus leptodactylus; Ecrevisse.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1185.pdf
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Effects of exposure to petroleum hydrocarbons upon the metabolism of the common sole Solea solea ArchiMer
Davoodi, Fariba; Claireaux, Guy.
On December 12th, 1999, the oil tanker Erika sank off the southern coast of Brittany (France), releasing 20 000–30 000 tons of heavy oil in the open sea. Among the affected coastal habitats were important nurseries for numerous flatfish species and particularly for the common sole, Solea solea. To investigate the potential impact of the spill on this economically significant resource, we employed Fry's concept of metabolic scope for activity to assess the deleterious effect of fuel exposure on the functional integrity of juvenile sole. Fish were captured from uncontaminated areas and experimentally exposed to contamination conditions mimicking those encountered during the weeks that followed the Erika spill. Using respirometry techniques we measured basal...
Tipo: Text Palavras-chave: Solea solea; Environmental adaptation; Metabolism; Fuel exposure; Erika oil spill.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2798.pdf
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Metabolic response to hypoxia in European sea bass (Dicentrarchus labrax) displays developmental plasticity ArchiMer
Cadiz, Laura; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Madec, Lauriane; Le Delliou, Herve; Mazurais, David.
Several physiological functions in fish are shaped by environmental stimuli received during early life. In particular, early-life hypoxia has been reported to have long-lasting effects on fish metabolism, with potential consequences for fish life history traits. In the present study, we examine whether the synergistic stressors hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), encountered during early life, could condition later metabolic response in European sea bass (Dicentrarchus labrax) juveniles. Growth rate and metabolic parameters related to carbohydrate and lipid metabolism in the liver were investigated at the juvenile stage under normoxic and chronic hypoxic conditions. Juvenile growth rates were significantly lower...
Tipo: Text Palavras-chave: Hypoxia; Developmental plasticity; Metabolism; European sea bass.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00404/51536/52126.pdf
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Activity and composition of the benthic fauna in the Whittard Canyon and the adjacent continental slope (NE Atlantic) ArchiMer
Duineveld, Gérard; Lavaleye, Marc; Berghuis, Eilke; De Wilde, Peter.
We compared the sediment and its community on the Celtic continental slope (Goban Spur) with those in a branch of the nearby Whittard Canyon in search for evidence of canyon mediated transport of (labile) organic matter. We studied the megabenthos and macrobenthos biomass and taxonomic composition, measured in situ sediment community oxygen consumption and determined sediment concentrations of particulate organic carbon, phytopigments, and nucleic acids. While the sediment community and activity on the canyon fan was similar to that on the abyssal station near Goban Spur, the sediment within the canyon had relatively enhanced sediment community oxygen consumption rates and higher levels of phytopigments. particulate organic carbon and nucleic acids,...
Tipo: Text Palavras-chave: Zone abyssale; Canyon; Macrofaune; Métabolisme; Pigments végétaux; Deep-sea; Canyon; Macrofauna; Metabolism; Phytopigments.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00323/43370/42840.pdf
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Biomass, metabolism and nutrition of zooplankton in the Gulf of Lions (NW Mediterranean) ArchiMer
Gaudy, R; Youssara, F; Diaz, F; Raimbault, P.
Zooplankton biomass was measured at 20 stations located along five coastal-offshore transects in the Gulf of Lions (NW Mediterranean) during spring 1998 and winter 1999, using two nets equipped with 200 and 80 Pm mesh. Biomass was twice as abundant in spring. It increased toward the coast and the west of the gulf and was positively correlated to chlorophyll, primary production and organic particulate matter and negatively to temperature and salinity. The enrichment effect of Rhone river input and the oligotrophic influence of the north Mediterranean current (NMC) were the main distribution factors of zooplankton biomass. Higher biomass was obtained in coastal stations with the 80 mum net, which sampled more efficiently young larval stages and nauplii....
Tipo: Text Palavras-chave: Zooplankton; Métabolisme; Cycle saisonnal; Golfe du Lion; Zooplankton; Metabolism; Seasonal cycle; Gulf of Lions.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00321/43262/42993.pdf
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Transferts d'acides amines dans un systeme eau-sediment: Etude preliminaire en milieu experimental ArchiMer
Hermin, M; Daumas, R; Drogue, N.
The bacterial activity of the superficial sediment layer was evaluated by incorporation super(14)C-labelled amino-acids mixed an hydrolysate of chlorella cells. Estimation of super(14)C-CO sub(2) super(14)C and super(14)C-A.A. by H.P.L.C. give some informations on the utilization of free dissolved. A.A. and on the transport of labelled compounds from water to sediment. The adsorption mechanism of A.A. on the mineral particles draw to the sediment a larger part of A.A. in the first period; but 28% of this adsorbed A.A. were discharged by an acid treatment. A part of super(14)C-A.A. was incorporated in unhydrolysable organic matter. Di-COOH A.A. are more resistant to bacterial degradation than the other A.A., but arginine is completely metabolized in ten...
Tipo: Text Palavras-chave: Biogeochemistry; Bacteria; Chromatographic techniques; Metabolism; Amino acids; Sediment water exchanges.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-945.pdf
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Short term variations in feeding and metabolism of Acartia tonsa (pelagic copepod) in the Berre lagoon (France) ArchiMer
Gaudy, R; Pagano, M; Cervetto, G; Saintjean, L; Verriopoulos, G; Beker, B.
Feeding, respiration, and ammonia excretion of Acartia tonsa were studied in a brackish lagoon, near Marseilles (Southern France). The experiments were performed during the same season in two successive years, using naturally occurring particles as food. Strong variations in both quantity and quality of food particles were recorded between the two series of experiments. The ingestion of particles according to their size was studied by means of the Coulter Counter technique. Females ingested more food than males. In both sexes, there was a tendency to select median to large particles, regardless of the shape of the particles-size spectrum. In 1990, the specific production rate, deduced from the energy budget, was equivalent to or greater than the observed...
Tipo: Text Palavras-chave: Acartia tonsa; Feeding; Metabolism; Production.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00096/20731/18368.pdf
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Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss) ArchiMer
Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf
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Cultivating the uncultured: limits, advances and future challenges ArchiMer
Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf
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Energy-protein management by some warmwater finfishes. ArchiMer
Luguet, P; Moreau, Y.
If warmwater finfish protein requirements as level in diet is low, absolute intake per day is similar to those of coldwater species. But relation of this requirement with specific growth rate differs for strictly warmwater fishes as Tilapias. Analysis of partition between protein and non-protein energy retention shows that these species with Clarias make a better use of non-protein energy provided and consequently improve their protein retention. Better carbohydrate digestibility and metabolism are propounded as part of the explanation.
Tipo: Text Palavras-chave: Clarias; Oreochromis mossambicus; Oreochromis aureus; Oreochromis niloticus; Tilapia; Cichlidae; Pisces; Nutritional requirements; Digestibility; Diets; Proteins; Metabolism; Carbohydrates; Nutrition; Feeding; Growth rate.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1424.pdf
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Influence of water temperature and oxygenation on the aerobic metabolic scope of Atlantic cod (Gadus morhua) ArchiMer
Claireaux, Guy; Webber, D; Lagardere, Jean-paul; Kerr, S.
Environmental influences (temperature and oxygenation) on cod metabolism and their impact on the ecology of this species were investigated. Limiting oxygen concentration curves (O-2 level ranging between 15 and 100% air saturation) were established at 2, 5 and 10 degreesC. The standard metabolic rate (SMR), the maximum metabolic rate and the metabolic score were then modelled as functions of temperature and/or oxygen saturation. The mean SMR at 2, 5 and 10 degreesC were 19.8 +/- 4.9, 30.8 +/- 6.1 and 54.3 +/- 4.1 mg O-2 h(-1) kg(-1). respectively. Between 2 and 5 degreesC, the active metabolic rate of cod almost doubled from 65 to 120 mg O-2 h(-1) kg(-1), to reach 177 mg O-2 h(-1) kg(-1) at 10 degreesC. In terms of metabolic scope (MS), the temperature...
Tipo: Text Palavras-chave: Oxygen; Temperature; Physiological ecology; Metabolism; Atlantic cod.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-428.pdf
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Maintenance of discrete bacterial populations in adjacent marine habitats. ArchiMer
Robb, F; Muir, D; Davis, C.
Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems...
Tipo: Text Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf
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Effects of O-2 supersaturation on metabolism and growth in juvenile turbot (Scophthalmus maximus L.) ArchiMer
Person, Jeannine; Pichavant, Karine; Vacher, Coralie; Le Bayon, Nicolas; Severe, Armelle; Boeuf, Gilles.
Effects of O-2 supersaturation on metabolism and growth were studied in juvenile turbot (Scophthalmus maximus L.). When fish were reared for 30 days in water containing O-2 at 147% or 223% air saturation, there were no significant differences in food intake, growth, food conversion or protein utilization compared to fish exposed to normoxia (100% air saturation in water outlet). Exposure to hyperoxia resulted in increased body fat deposition. Daily rates of O-2 consumption of resting fish were not affected by O-2-concentrations, and there were no significant differences in rates of nitrogenous excretion among fish exposed to the different O-2-concentrations. Turbot tolerated severe hyperoxia, 350% air saturation, for 10 days.There were changes in acid-base...
Tipo: Text Palavras-chave: Ion regulation; Acid base; Metabolism; Growth; Hyperoxia; Turbot.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-627.pdf
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