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Registros recuperados: 27
Primeira ... 12 ... Última
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Effectiveness of Metarhizium anisopliae against immature stages of Anastrepha fraterculus fruitfly (Diptera : Tephritidae) BJM
Destéfano,Ricardo Henry Rodrigues; Bechara,Ivanira José; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico.
The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales) strain E9, isolated from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae), against larvae, prepupae and pupae stage and emergent adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on autoclaved (AS) and non-autoclaved (NAS) soil from typical citrus orchards in the State of São Paulo, Southeastern region of Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10) for AS and 2.52 x 10(10) for NAS. The lethal concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial control; Metarhizium anisopliae; Anastrepha fraterculus; Fruitfly; Bioassay.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100018
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Sporulation of Metarhizium anisopliae var. Acridum and Beauveria bassiana on Rhammatocerus schistocercoides under humid and dry conditions BJM
Magalhães,Bonifácio Peixoto; Goettel,Mark Stanislaw; Frazão,Heloisa da Silva.
The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R. schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity. Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Entomopathogenic fungi; Internal sporulation; Grasshoppers; Microbial control.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300002
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Mechanism of infection and colonization of Rhipicephalus sanguineus eggs by Mertarhizium anisopliae as revealed by scanning eletron microscopy and histopathology BJM
Garcia,Marcos Valerio; Monteiro,Antonio Carlos; Szabo,Matias Juan Pablo; Prette,Nancy; Bechara,Gervásio Henrique.
The present work aimed to verify the penetration pattern of the Metarhizium anisopliae fungus into Rhipicephalus sanguineus tick eggs, as well as the lesions occurred inside the egg. The fungus adherence and penetration were studied by scanning electron microscopy and the action of fungus in the internal tissues was evaluated through conventional histological sessions. For the observation of these events, experimental infections were performed in 11 groups of R. sanguineus eggs containing 25 mg each. The eggs were bathed during 3 minutes under manual shaking in a 10(8) conidia/mL suspension. The bath was performed only in the suspension vehicle for the control groups. The eggs were processed for histopathological analysis and scanning electron microscopy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Ticks.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400012
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Total lipids and fatty acids of strains of Metarhizium anisopliae BJM
Pupin,Antonio Marcos; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico; Roberts,Donald Wilson.
Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var. majus were similar in percentages of total lipids. Tukey test for average of lipid content in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p= 1%) with the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipids; Fatty acids; Metarhizium anisopliae; Entomopathogenic fungi; Microbial control.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200012
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Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae) BJM
Pria Júnior,Wolney Dalla; Lacava,Paulo Teixeira; Messias,Claudio Luiz; Azevedo,João Lúcio; Lacava,Pedro Magalhães.
Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10(7) conidia mL-1. Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (P<0.05). These significant differences increased until 10 days after treatment. The fungus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial control; Entomopathogen; Citrus Variegated Chlorosis (CVC); Bioassay.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100027
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Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis BJM
Tigano,Myrian Silvana; Aljanabi,Salah; Mello,Sueli Corrêa Marques de.
The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities computed from RAPD markers showed two distinct genetic groups of isolates related to both species. RAPD analysis proved to be an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in S. obtusifolia, and also for distinguishing Alternaria species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Senna obtusifolia; Bioherbicide; Microbial control.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200005
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Influence of culture media in viability test of conidia of entomopathogenic fungi Ciência Rural
Francisco,Edimara Aparecida; Mochi,Dinalva Alves; Correia,Antônia do Carmo Barcelos; Monteiro,Antonio Carlos.
This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beauveria bassiana; Lecanicillium lecanii; Paecilomyces fumosoroseus; Germination; Microbial control.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043
Registros recuperados: 27
Primeira ... 12 ... Última
 

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