SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Preliminar assay to establish a micropropagation protocol for mango (Mangifera indica, L.) cultivated in brazilian savannah.

LEDO, A. da S.; LAMEIRA, O. A.; MOURA, E.; MENEZES, I. C. de.

bitstream/item/42519/1/Boletim-Pesquisa-28.pdf

Tipo: Séries anteriores (INFOTECA-E)

Palavras-chave: Cupuaçuzeiro; Belém; Pará; Brasil.; Cultura de Tecido; Cultura In Vitro; Micropropagação; Theobroma Grandiflorum.; Amazonia; In vitro culture; Micropropagation; Tissue culture..

Ano: 2000

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382729

Desenvolvimento e avaliação de protocolos para descontaminação de explantes de mangueira visando à micropropagação.

CORDEIRO, M. C. R.; BARRUETO, C. P. L.; PITO, A. C. Q.; GOMES, A. C.; ANDRADE, S. R. M.; VARGAS RAMOS, V. H..

bitstream/CPAC-2009/24171/1/p2002_60.pdf

Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)

Palavras-chave: Tropical fruits.; Explante; Fruta Tropical; Manga; Mangifera Indica; Micropropagação; Melhoramento Vegetal; Propagação Vegetativa.; Explants; Plant propagation.; Micropropagation; Plant breeding.

Ano: 2004

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567480

ANDRADE, S. R. M. de; PINTO, A. C. de Q.; FALEIRO, F. G.; CORDEIRO, M. C. R.; RAMOS, V. H. V.; TEIXEIRA, J. B..

RESUMO: A eliminação de microrganismos é uma condição essencial para o estabelecimento de um processo de regeneração in vitro, pois eles além de competirem pelos nutrientes também produzem toxinas que causam a morte do explante. O trabalho visou desenvolver metodologias de controle de crescimento de microrganismos provenientes de explantes de mangueira inoculados em meio de cultura, como fase inicial do estabelecimento de um protocolo de micropropagação de gemas laterais de mangueira para apoiar o programa de melhoramento desenvolvido na Embrapa. Contudo, uma bactéria de cor esbranquiçada, gram positiva, demonstrou ser resistente a todos os antibióticos, menos ao complexo sulfametaxazol + trimetroprima. Foi verificada a presença de outra bactéria, de...

Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)

Palavras-chave: Mango; Desinfection.; Cultura de Tecido; Desinfecção; Explante; Genética Vegetal; Manga; Mangifera Indica; Organogénese.; Genetics; Explants; Micropropagation; Tissue culture..

Ano: 2005

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570345

Embriogênese somática e regeneração de plantas em açaizeiro.

LEDO, A. da S.; LAMEIRA, O. A.; MENEZES, I. C. de.

O objetivo do presente trabalho foi estudar as diferentes respostas morfogenéticas de embriões zigóticos de açaizeiro (Euterpe oleracea Mart.) submetidos a várias condições de cultura in vitro. Os experimentos foram conduzidos em laboratório com material vegetal coletado de plantas de açaí na Embrapa Amazônia Oriental, Belém, PA, Brasil. Verificou-se a expressão de um modelo de embriogênese somática, direto, repetitivo a assincronizado em embriões zigóticos maduros cultivados em meio primário MS suplementado com 339.36 ? M de 2,4-diclorofenoxiacético (2,4-D) e transferidos para meio secundário MS na presença de 0,537 ? M de ácido 1-naftalenoacético (ANA) e 12,30 ? M de 2-isopenteniladenina (2iP). A conversão de embriões somáticos em plântulas foi alcançada...

Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)

Palavras-chave: Açaí de touceira; Embrapa Amazônia Oriental; Belém (PA); Pará; Eastern Amazon; Amazônia Oriental; Micropropagación.; Cultivo in vitro; Fitomejoramiento; Embriogénesis; Melhoramento genético vegetal; Açaí; Euterpe oleracea; Cultura in vitro; Embriogênese.; Micropropagação; Embryogenesis.; Micropropagation; Plant breeding; In vitro culture.

Ano: 2002

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/496030

Cultura de tecidos: (manual).

Produção de mudas de teca por micropropagação.

LAMEIRA, O. A.; LEMOS, O. F.; MENEZES, I. C. de; PINTO, J. E. B. P..

bitstream/item/63281/1/Oriental-Doc66.pdf

Tipo: Documentos (INFOTECA-E)

Palavras-chave: Manual.; Cultura de Tecido; Micropropagação.; Micropropagation; Tissue culture..

Ano: 2000

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382772

RAPOSO, A.; FERMINO JUNIOR, P. C. P.; TEIXEIRA, R. B.; PEREIRA, J. E. S..

A propagação in vitro de teca tem sido realizada por meio da cultura de tecidos vegetais de diferentes genótipos. Nesse contexto, o uso da micropropagação a partir de plantações de teca no Brasil pode ser uma importante ferramenta, pois permite a produção de grandes quantidades de novas plantas em um curto espaço de tempo, a partir de um único propágulo vegetativo retirado de uma planta matriz. Assim, o objetivo deste trabalho é descrever protocolos de micropropagação da espécie Tectona grandis a partir de genótipos estabelecidos na Amazônia Sul-Ocidental, no Estado do Acre.

Tipo: Circular Técnica (INFOTECA-E)

Palavras-chave: Teak; Producción de plántulas.; Cultivo in vitro; Madera tropical; Micropropagación; Tectona grandis; Muda; Produção.; Cultura in vitro; Essência florestal; Teca; Micropropagação; Tropical wood; Seedling production.; In vitro culture; Micropropagation.

Ano: 2010

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/872550

Automacao e racionalizacao na micropropagacao industrial.

Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia

FARI, M.; MELO, N. F. de.

Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.

Tipo: Artigo de divulgação na mídia (INFOTECA-E)

Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.

Ano: 1996

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977

Almeida,Marcílio de; Almeida,Cristina Vieira de.

The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.

Acclimatization of Tapeinochilos ananassae plantlets in association with arbuscular mycorrhizal fungi

Palavras-chave: Bactris gasipaes; Histology; Micropropagation; Peach palm.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000900015

Oliveira,João Ricardo Gonçalves de; Morais,Thiago Alberto de Lima; Melo,Natoniel Franklin de; Yano‑Melo,Adriana Mayumi.

The objective of this work was to assess the potential of three isolates of arbuscular mycorrhizal fungi to promote growth of micropropagated plantlets of Tapeinochilos ananassae during acclimatization. The experiment was carried out in greenhouse, in a completely randomized block design, with four inoculation treatments: non‑inoculated control and plants inoculated with Glomus etunicatum, Acaulospora longula or Gigaspora albida, with ten replicates. After 90 days, the following parameters were evaluated: survival rate, height, leaf and tiller number, leaf area, fresh and dry biomass, contents of macro‑ and micronutrients in the root and shoot, glomerospore number, and mycorrhizal colonization. The survival percentage was 100%, except for plants inoculated...

Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao

Palavras-chave: Glomeromycota; Growth promotion; Micropropagation; Mineral nutrition; Tropical flowers.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900018

Silva,Thiago Édson Ribeiro da; Cidade,Luciana Cardoso; Alvim,Fátima Cerqueira; Cascardo,Júlio Cézar de Mattos; Costa,Marcio Gilberto Cardoso.

The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.

ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT

Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000024

GLORIA,BEATRIZ APPEZZATO DA; VIEIRA,MARIA LUCIA CARNEIRO; DORNELAS,MARCELO CARNIER.

With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001100005

Silva,Tatiane Loureiro da; Scherwinski-Pereira,Jonny Everson.

The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of...

Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

Palavras-chave: Ex situ conservation; Germplasm bank; Micropropagation.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007

Gagliardi,Rachel Fatima; Pacheco,Georgia Pereira; Oliveira,Carlos Alberto; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Vieira,Maria Lucia Carneiro; Mansur,Elisabeth.

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

Silicon and agar on in vitro development of cockscomb (Amaranthaceae)

Palavras-chave: Germplasm conservation; Micropropagation; Molecular characterization; Genetic stability.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200015

Assis,Franscinely Aparecida de; Souza,Genaina Aparecida de; Dias,Gabrielen de Maria Gomes; Assis,Gleice Aparecida de; Rodrigues,Filipe Almendagna; Pasqual,Moacir; Costa,Bárbara Nogueira Souza; Carvalho,Fabio Janoni.

Abstract: The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige & Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this...

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Palavras-chave: Celosia cristata; Gelling agent; Micropropagation; Ornamental plant; Silicic acid.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100030

Silva,Rafael de Carvalho; Luis,Zanderluce Gomes; Scherwinski-Pereira,Jonny Everson.

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...

Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa

Palavras-chave: Vitis; Germplasm; Ex situ conservation; In vitro conservation; Micropropagation; Minimal growth.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000300005

Pacheco,Georgia; Gagliardi,Rachel Fátima; Cogliatti,Mariana Buturini; Manhães,Harlen Barreira; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Mansur,Elisabeth.

The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.

Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100023

Rodrigues,Filipe Almendagna; Rezende,Renata Alves Lara Silva; Pasqual,Moacir; Lopes,Maria Teresa Gomes.

Abstract: The objective of this work was to evaluate the effects of the solidifying agents agar and phytagel and of activated charcoal on the in vitro cultivation of two maná cubiu (Solanum sessiliflorum) varieties: Thaís and Santa Luzia. The phytotechnical characteristics analyzed included number of leaves, number of roots, shoot and root length, and fresh matter of shoot and root. Regardless of the variety, phytagel was superior to agar as a culture medium. A greater number of leaves and longer shoots were observed in the Santa Luzia variety, in the absence of charcoal. The Thaís variety showed longer shoots and roots in the presence of charcoal.

Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant

Palavras-chave: Maná cubiu; Micropropagation; Plant tissue culture; Phytagel; Solanaceae.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001101123

Phyton

Giménez,C; de García,E; Haddad,O.

Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with 10 different primer combinations. Non-polymorphic RAPD bands were...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572008000100006

Palavras-chave: Micropropagation; Genetic stability; RAPD; Disease resistance; Youngest leaf spotted (YLS).

Ano: 2008

An efficient protocol for culturing meristems of sorghum hybrids

Phyton

Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV.

A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010

Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes.

Ano: 2010

Somatic embryogenesis in goiabeira serrana: genotype response, auxinic shock and synthetic seeds

Rev. Bras. Fisiol. Veg.

GUERRA,MIGUEL P.; DAL VESCO,LIRIO L.; DUCROQUET,JEAN PIERRE H.J.; NODARI,RUBENS O.; REIS,MAURÍCIO S. DOS.

The induction and control of in vitro somatic embryogenesis is dependent of a number of factors. The genotype of the mother plant donor of explants and the kind of auxin play essential roles in conferring embryogenic competence. Once obtained somatic embryos may be encapsulated in alginate originating synthetic seeds that can be stored at low temperatures. Considering this the present work aimed at the assessment of five different genotypes, five periods of 2,4-D shock (20 muM for 1, 2, 4, 8 and 16 weeks) (), and different substances supplemented to sodium alginate to obtain synthetic seeds. Zygotic embryos of Feijoa sellowiana were inoculated in basal LP medium (von Arnold and Erikson, 1981) supplemented with Morels vitamins, sucrose (3%) and phytagel...