SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Silva,S.E.L. da; Bonetti,A.M.; Petrocelli,A.; Ferrari,H.F.; Luvizotto,M.C.R.; Cardoso,T.C..

A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileum-caeca region were collected from 30-day-old poults suffering of enteritis episode characterized as poult enteritis mortality syndrome (PEMS). The PEMS clinical features were characterized by watery to foamy faeces, light brown-yellow in colour and low mortality rate. Meteorological data (rainfall and relative humidity) observed during...

Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction

Palavras-chave: Turkey Astrovirus; Turkey Coronavirus; Molecular diagnosis; PEMS.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200008

Sepúlveda,Lya Madureira; Malirat,Viviana; Bergmann,Ingrid E.; Mantilla,Anibal; Nascimento,Elmiro Rosendo do.

Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the polymerase...

Application of broad-range bacterial PCR amplification and direct sequencing on the diagnosis of neonatal sepsis

Palavras-chave: Polymerase Chain reaction; Vesicular Stomatitis; Molecular diagnosis.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300022

Ruppenthal,Rubia Denise; Pereira,Fabiana de Souza; Cantarelli,Vlademir Vicente; Schrank,Irene Silveira.

A broad-range bacterial PCR target to conserved regions of the 23S rDNA was applied to 306 blood culture samples from 295 infants (up to one year of age) admitted to a neonatal intensive care unit. Classic blood culture results were compared to DNA sequencing analysis of the PCR amplification products. Culture results were in agreement to DNA sequencing in 90.5% (277) of 306 samples tested, including 263 PCR and culture negative samples and 29 culture and PCR positive samples. The sensitivity of the PCR method combined with sequencing was 88%, and the specificity was 96.3%, with positive and negative predictive values of 74.3 e 98.5%, respectively. The PCR-based approach directly applied to blood culture samples, correlated well with blood culture results...

Clinical and epidemiological use of nested PCR targeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii

Palavras-chave: Sepsis; Molecular diagnosis; Neonatal; PCR.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100006

Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S..

ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the...

Canine distemper virus detection by different methods of One-Step RT-qPCR

Palavras-chave: Q fever; Coxiella burnetii; Molecular diagnosis; Nested PCR; IS1111.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100138

Tozato,Claudia de Camargo; Zadra,Vívian Ferreira; Basso,Caroline Rodrigues; Araújo Junior,João Pessoa.

ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A) and a One-Step RT-qPCR combined with NESTED-qPCR (system B). Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100%) urine samples from...

Pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil

Palavras-chave: Canine distemper; Molecular diagnosis; One-Step RT-qPCR; Urine; Centrifugation.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901601

Bianchi,Ronaldo Michel; Cecco,Bianca Santana de; Schwertz,Claiton Ismael; Panziera,Welden; Andrade,Caroline Pinto de; Spanamberg,Andreia; Ravazzolo,Ana Paula; Ferreiro,Laerte; Driemeier,David.

ABSTRACT: This report describes the clinical, pathological, and molecular aspects of a pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil. A goat with a history of prolonged transportation presented dyspnea, nasal discharge and cough, and was subjected to necropsy, histopathology, and mycological evaluation. Grossly, cranio-ventral pulmonary consolidation was observed, characterized by firm and grayish areas interspersed with dark red foci. Histopathology of the lungs showed areas of parenchymal necrosis, containing blastoconidia with a slightly basophilic central cell, surrounded by an unstained capsule. It was associated with moderate granulomatous inflammatory infiltrate and peripheral fibrosis. The capsule and fungus cell...

Fatal systemic Mortierella wolfii infection in a neonatal calf in southern Brazil

Palavras-chave: Goats; Respiratory diseases; Pathology; Mycology; Molecular diagnosis.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000551

Bianchi,Ronaldo Michel; Vielmo,Andréia; Schwertz,Claiton Ismael; Cabreira,Adan; Rocha,Jéssica Goulart da; Siqueira,Franciele Maboni; Pavarini,Saulo Petinatti.

ABSTRACT: This study described the pathological and microbiological aspects of a fatal systemic Mortierella wolfii infection in a neonatal calf in southern Brazil. The calf was born clinically normal, but on the third day of life it presented apathy, unilateral hypopyon, and neurological signs, and in the next day it was euthanized. At necropsy, multiple soft, and white-yellow nodules were observed in the liver, spleen, kidneys, mesenteric lymph nodes, heart, and lungs. In the brain, reddish, and friable areas were found. In the eye, there were anterior and posterior synechiae, diffuse thickening of choroid, and anterior chamber filled by whitish and friable material. Microscopically, areas of necrosis, pyogranulomatous inflammation, vasculitis,...

Oral infection of neonate gerbils by Neospora caninum tachyzoites

Palavras-chave: Cattle diseases; Fungal diseases; Pathology; Molecular diagnosis.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100551

Ferreira,Maiara Sanitá Tafner; Vogel,Fernanda Silveira Flores; Sangioni,Luis Antonio; Weber,Augusto; Bräunig,Patricia; Vaz,Marcos André Braz; Cezar,Alfredo Skrebsky.

ABSTRACT: Neosporosis is a parasitic disease caused by the protozoan Neospora caninum which results in major economic losses for cattle breeding due to abortion and other reproductive disorders. Gerbils (Meriones unguiculatus) are commonly used as experimental models for neosporosis due to their high susceptibility to N. caninum infection, both by oocysts ingestion as by tachyzoites/bradyzoites parenteral inoculation. However, the risk of transmission by tachyzoites ingestion is not fully elucidated. In this study, infection of neonate gerbils by N. caninum (NC-1 strain) tachyzoites inoculated by the oral route and the parasite distribution in gerbils' tissues were evaluated by protozoan DNA detection. Seventeen neonate gerbils, aged 4-5 days, were...