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| Registros recuperados: 26 | |
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| Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi. |
| Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004 |
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| Ramos,Daniela Fernandes; Silva,Ana Bárbara Scholante; Fagundes,Michel Quevedo; von Groll,Andrea; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio. |
| Bovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the frequent movement of cattle between different properties. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine tuberculosis; Mycobacterium bovis; Genotyping. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200039 |
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| Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de Souza. |
| Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Tuberculosis; Mycobacterium bovis; Mycobacterium avium paratuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016 |
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| Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina; Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías. |
| The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013 |
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| Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Filho,Antônio Francisco de Souza; Cárdenas,Nicolás Céspedes; Alves,Clebert José; Azevedo,Sérgio Santos de. |
| ABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Mycobacterium bovis; Isolation; Milk; PCR; Cattle. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202166 |
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| Registros recuperados: 26 | |
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