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| Registros recuperados: 31 | |
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| Lima,Isabela Angeli de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara. |
| ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nanoparticles; Ferulic acid/encapsulation efficiency; Ferulic acid/quantification; Stability; High performance liquid chromatography/validation; PLGA-poly(lactide-co-glycolide)/nanoparticles; Chitosan.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200627 |
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| Guan,Jiao; Han,Liqin; Shi,Nianqiu; Zhu,Heyun; Wang,Junmin. |
| Docetaxel-loaded acetic acid conjugated Cordyceps sinensis polysaccharide (DTX-AA-CSP) nanoparticles were prepared through dialysis and their release rates in vitro, particle sizes, zeta potentials, drug loading capacities, and encapsulation efficiencies were characterized for the synthesis of AA-modified CSPs from traditional Chinese medicine Cordyceps sinensis (Berk.) Sacc. Then, the AA-modified CSPs were characterized by 1H-NMR and FT-IR. Furthermore, the biocompatibility of the delivery carrier (AA-CSP nanoparticles) was assessed on human umbilical vein endothelial cells. In vitro antitumor activity studies on DTX-AA-CSP nanoparticles were conducted on the human liver (HepG2) and colon cancer cells (SW480). The DTX-AA-CSP nanoparticles were spherical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Docetaxel; Cordyceps sinensis polysaccharide; Nanoparticles; Antitumor efficacy; Biocompatibility. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100599 |
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| Dashamiri,Somayeh; Ghaedi,Mehrorang; Naghiha,Reza; Salehi,Amin; Jannesar,Ramin. |
| As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles. The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with Mn-Ni@Fe3O4-NPs & Mn: Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Aspergillus oryzae, and Candida albicans. The effects of antimicrobial activities on above scenarios were evaluated using disc diffusion, MIC, MBC, and E. coli DNA electrophoresis methods. The results showed that the effects of antibacterial assay values of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Euphorbia prostrata/extract/antimicrobial activities; Pelargonium graveolens/extract/antimicrobial activities E coli; Candida albicans; Nanoparticles. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400625 |
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| Ianiski,Francine Rodrigues; Laporta,Luciane Varini; Rubim,Alexandre Machado; Luchese,Cristiane. |
| abstract A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High performance liquid chromatography/quantitative analysis; Meloxicam/determination; PEGylated nanocapsules/quality control; Nanoparticles; Poly(ethylene glycol).. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400823 |
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| Vijayaraj,Surendran; Palei,Narahari N; Archana,Devalapalli; Lathasri,Kuppam; Rajavel,Ponnusamy. |
| Rutin is a flavonoid glycoside, mainly consists of phenolic compounds, responsible for many biological activities. The objective of the present study was to develop and validate a precise, simple, robust, rapid and reliable reverse phase high -performance liquid chromatography (RP-HPLC) technique by using Qbd approach for evaluating the rutin in nanoparticles. Central composite design (CCD) was employed for optimizing the experimental conditions of RP-HPLC method. Buffer pH, methanol content in the mobile phase composition, flow rate, and wavelength were selected as independent variables whereas retention time, peak area, and asymmetry factor was selected as dependent variables. The retention time, peak area and asymmetric factor of rutin by using... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rutin; Chitosan; Sodium aliginate; Nanoparticles; RP-HPLC; Central composite design; QbD. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100632 |
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| Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus. |
| Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623 |
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| Rodrigues,Caroline Danziato; Khalil,Najeh Maissar; Mainardes,Rubiana Mara. |
| In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amphotericin B/determination; Amphotericin B/encapsulation; High performance liquid chromatography/reverse phase/quantitative analysis; Nanoparticles. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859 |
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| Registros recuperados: 31 | |
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