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| Registros recuperados: 170 | |
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| Davenel, Armel; Quellec, Stéphane; Pouvreau, Stephane. |
| The aim of this study was to test the ability of magnetic resonance imaging (MRI) technique to characterize gonad development and to determine the sex of live Pacific oysters through their shells. A preliminary nuclear magnetic resonance (NMR) retaxometry study was conducted to characterize T-1 and T-2 NMR relaxation parameters for the main oyster organs. This showed that T-1-weighted MRI sequences were most appropriate to optimize contrasts between tissues in images. The results showed that gray levels of gonads in images acquired with gradient-echo sequence were variably affected by T-2* weighting effect. However, the ovaries systematically gave a hypersignal in spin-echo T-1-weighted images, and stack histograms of female oysters showed a peak well... |
| Tipo: Text |
Palavras-chave: Spin echo; Gradient echo; Sex determination; Gonad maturation; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1987.pdf |
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| Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida. |
| Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore... |
| Tipo: Text |
Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf |
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| Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc. |
| Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing... |
| Tipo: Text |
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf |
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| Bado-nilles, Anne; Auffret, Michel; Quentel, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène. |
| Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune... |
| Tipo: Text |
Palavras-chave: PaHs; Contamination; Crassostrea gigas; Oyster. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3428.pdf |
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| Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp. |
| Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large... |
| Tipo: Text |
Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf |
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| Heral, Maurice; Bayne, B.. |
| The Workshop was held in Plymouth (6-10 October 1996) in the Royal Plymouth Corinthian Yacht club organised by the Plymouth Marine Laboratory under the joint charmanships of B. Bayne et M. Héral. 36 scientists were participated to this meeting from 11 countries, 8 belonging to ICES (Annex 1) 30 communications have been presented. This meeting was supported by an European Union contrat from DG14 with a concerted action AIR3 CT 942219 on Trophic capacity of coastal zone of oysters, mussels and cockles cultivation. This contract has paid the travel cost of all the participants. |
| Tipo: Text |
Palavras-chave: Coastal systems; Carrying capacity; Physiological results; Oyster; Shefflish bivalve. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/1996/acte-7350.pdf |
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| Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
| The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
| Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
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| Rondon, R.; Akcha, Farida; Alonso, P.; Menard, Dominique; Rouxel, Julien; Montagnani, Caroline; Mitta, Guillaume; Cosseau, C.; Grunau, C.. |
| The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes... |
| Tipo: Text |
Palavras-chave: Mollusk; Oyster; Diuron; Transcriptomic; Ecotoxicology. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00318/42938/42438.pdf |
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| Benabdelmouna, Abdellah; Ledu, Christophe. |
| We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the... |
| Tipo: Text |
Palavras-chave: Oyster; Tetraploid induction; Mosaicism; Aneuploidy; Cytogenetic stability. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00278/38924/37464.pdf |
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| Tallec, Kevin; Huvet, Arnaud; Di Poi, Carole; Gonzalez-fernandez, Carmen; Lambert, Christophe; Petton, Bruno; Le Goic, Nelly; Berchel, Mathieu; Soudant, Philippe; Paul-pont, Ika. |
| In the marine environment, most bivalve species base their reproduction on external fertilization. Hence, gametes and young stages face many threats, including exposure to plastic wastes which represent more than 80% of the debris in the oceans. Recently, evidence has been produced on the presence of nanoplastics in oceans, thus motivating new studies of their impacts on marine life. Because no information is available about their environmental concentrations, we performed dose-response exposure experiments with polystyrene particles to assess the extent of micro/nanoplastic toxicity. Effects of polystyrene with different sizes and functionalization (plain 2-μm, 500-nm and 50-nm; COOH-50 nm and NH2-50 nm) were assessed on three key reproductive steps... |
| Tipo: Text |
Palavras-chave: Oyster; Embryos; Gametes; Microplastics; Nanoplastics. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56419/58090.pdf |
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| Richard, Marion; Forget, Fabien; Mignucci, Alexandre; Mortreux, Serge; Le Gall, Patrik; Callier, Myriam; Weise, Am; Mckindsey, Cw; Bourjea, Jerome. |
| Bivalve predation by seabream has been observed worldwide and is a major concern for bivalve farmers. Farmed bivalve-seabream interactions must be better understood to ensure the sustainability of bivalve aquaculture. The objectives of this study were to characterize gilthead seabream Sparus aurata presence in a bivalve farm in Prevost Lagoon (Mediterranean Sea) using acoustic telemetry and to evaluate monthly losses of mussels Mytilus galloprovincialis and oysters Crassostrea gigas due to seabream predation over an 18 mo period inside the farm and at an unprotected experimental platform. Large (281 to 499 mm TL) seabream were more commonly detected in the bivalve farm than were small (200 to 280 mm TL) seabream. In contrast to small seabream, 90% of large... |
| Tipo: Text |
Palavras-chave: Shellfish culture; Sparus aurata; Size; Acoustic telemetry; Crassostrea gigas; Mytilus galloprovincialis; Oyster; Mussel. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00663/77515/79299.pdf |
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| Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene. |
| Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf |
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| Thomas, Yoann; Cassou, Christophe; Gernez, Pierre; Pouvreau, Stephane. |
| Beyond key ecological services, marine resources are crucial for human food security and socio-economical sustainability. Among them, shellfish aquaculture and fishing are of primary importance but become more vulnerable under anthropogenic pressure, as evidenced by reported mass mortality events linked to global changes such as ocean warming and acidification, chemical contamination, and diseases. Understanding climate-related risks is a vital objective for conservation strategies, ecosystems management and human health. We provide here a comprehensive study of the historical mortality of adult oysters related to observed climate variability along the French Atlantic coast from 1986 to 2015, and we built on this knowledge to develop hindcast and forecast... |
| Tipo: Text |
Palavras-chave: North Atlantic; Weather regime; Oyster; Monitoring network; Mortality risk assessment; Climate models; RCP scenarios. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57255/59281.pdf |
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| Degremont, Lionel; Soletchnik, Patrick; Boudry, Pierre. |
| This study reports mortality under laboratory conditions in unselected controls and two lines of juvenile Pacific oysters Crassostrea gigas, previously selected for their high or low survival in the field during the summer period. Oysters were also deployed in field conditions and mortality between both conditions was then compared. In the laboratory, mortality was observed in all experiments and it always lasted for a week indicating that mortality under laboratory conditions was a short-term event. It was also shown that mortality could be repeated for a batch in several experiments using oysters which never experienced any abnormal mortality. This approach could facilitate further studies to investigate the causes of mortality by allowing repeated... |
| Tipo: Text |
Palavras-chave: Summer mortality; Crassostrea gigas; Laboratory; Field; Oyster; Juvenile. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00013/12432/9228.pdf |
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| Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan. |
| OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves... |
| Tipo: Text |
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf |
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| Registros recuperados: 170 | |
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