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| Registros recuperados: 77 | |
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| Botaro,Bruno Garcia; Lima,Ygor Vinícius Real de; Cortinhas,Cristina Simões; Silva,Luís Felipe Prada e; Rennó,Francisco Palma; Santos,Marcos Veiga dos. |
| The objective of this study was to evaluate the effect of genetic polymorphism of kappa-casein, breed and seasonality on the physicochemical characteristics, composition and stability of milk in commercial dairy herds. A total of 879 milk and blood samples were collected from 603 Holstein and 276 Girolando cows, obtained during rainy and dry seasons. Milk samples were analyzed to determine the physicochemical characteristics, composition and ethanol stability, while blood samples were subjected to polymerase chain reaction to identify the kappa-casein genotype. The frequencies of genotypes AA, AB and BB of k-casein were respectively, 66.83, 31.84 and 1.33% for Holstein, and 71.38, 27.90 and 0.72% for the Girolando cows, respectively. The A allele was more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crioscopy; Dairy cow; Ethanol stability test; Genetic variant; Polymerase chain reaction; Titratable acidity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001200022 |
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| NOVOA HERRÁN,Sandra Susana; CASTELBLANCO,Mariela; SÁNCHEZ -GÓMEZ,Myriam; UMAÑA PÉREZ,Adriana. |
| ABSTRACT Invasion of trophoblast into endometrium is vital for successful pregnancy development. MMP9 and uPA are key proteases in this process, but it is still not clear the regulation of its expression by Transforming Growth Factor Beta (TGF-β), a known negative regulator of trophoblast invasion. We evaluated the effect of TGF-β on the transcriptional expression of uPA and MMP9 over time, in HTR-8/SVneo trophoblast cells cultured with or without 0.5 % fetal bovine serum, via RT qPCR. The involved transcription factors and signaling pathways were analyzed in silico, using Proscan, Enrich, PCViz and Wiki Pathway. Results showed that TGF-β temporarily regulates the expression of uPA and MMP9. Serum modified the nature of TGF-β's effects on uPA expression,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Computational biology; Matrix metalloproteinase; Placenta; Plasminogen; Polymerase chain reaction. |
| Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000100026 |
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| Joaquim,S.F.; Guimarães,F.F.; Salina,A.; Junqueira,N.B.; Gomes,E.N.; Langoni,H.. |
| ABSTRACT Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds’ animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk quality; Polymerase chain reaction; Dairy herds. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601793 |
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| Scodro,Regiane Bertin de Lima; Reinhold-Castro,Kárin Rosi; Dias-Sversutti,Alessandra de Cassia; Neitzke-Abreu,Herintha Coeto; Membrive,Norberto de Assis; Kühl,João Balduíno; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei. |
| The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutaneous leishmaniasis; Sandflies; Dissection; Polymerase chain reaction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007 |
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| Oliveira,Micheline de Lucena; Amorim,Melania Maria Ramos de; Souza,Paulo Roberto Eleutério de; Albuquerque,Lúcia Cristina Bezerra de; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima. |
| This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chlamydia trachomatis; Chlamydia infections; Fluorescent antibody technique direct; Polymerase chain reaction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400014 |
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| Machado,Márcia Sacramento Cunha; Silva,Bruno Fernando Borges da Costa e; Gomes,Igor Logetto Caetité; Santana,Iuri Usêda; Grassi,Maria Fernanda Rios. |
| The incidence of sexually transmitted diseases among adolescents is increasing worldwide. Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in young women, and undetected disease is highly associated with long-term complications in women. Our goal was to determine the prevalence of cervical Chlamydia trachomatis infection in a sexually active population of female adolescents from Salvador, Brazil, and to describe their socio-demographic, behavioral, and clinical characteristics. 100 sexually active adolescents (10-19 years) were included in this study, between 2008 and 2010. Endocervical samples were obtained during gynecological examination. Inhouse polymerase chain reaction of cervical specimens was used... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Chlamydia trachomatis; Polymerase chain reaction; Adolescent. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200015 |
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| Lamas,Cristiane; Favacho,Alexsandra; Rozental,Tatiana; Bóia,Márcio N.; Kirsten,Andrei H.; Guterres,Alexandro; Barreira,Jairo; Lemos,Elba Regina S. de. |
| A lethal case of Brazilian spotted fever (BSF) is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA) for spotted fever group rickettsia (SFGR) was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR) and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian spotted fever; Spotted fever group rickettsia; Rickettsia rickettsii; Lethal case; Rio de Janeiro city; Indirect immunofluorescence; Polymerase chain reaction; Central nervous system involvement. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200010 |
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| Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. |
| Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 |
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| Oliveira,Diego Molina de; Lonardoni,Maria Valdrinez Campana; Teodoro,Ueslei; Silveira,Thais Gomes Verzignassi. |
| OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Polymerase chain reaction; Leishmania; DNA primers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300004 |
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| Registros recuperados: 77 | |
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