SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Exploitation of wild relatives of pigeonpea and chickpea for resistance to Helicoverpa armigera

Mallikarjuna, N..

Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.

Ano: 2006

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177

Mallikarjuna, N.,( Nalini Mallikarjuna ).

Palavras-chave: Pigeonpeas; Chickpeas; Hybridization; Genes; Gene pools; Interspecific hybridization; Hybrids; Genomes; Gene transfer; Polyploidy.

Ano: 2007

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3283

International Workshop on Groundnuts

Use of 2n pollen in generating interspecific derivatives of groundnut

R.W.( Editor ).

Palavras-chave: Groundnuts; Root nodulation; Genotypes; Biological interaction; Mycotoxins; Chromosomes; Fungi; Breeds (animals); Polyploidy.

Ano: 1980

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3978

Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion

Mallikarjuna, N..

Palavras-chave: Genomes; Polyploidy; Nucleus; Hybrids; Chromosome number; Chromosomes; Mosses; Meiosis; Cytogenetics; DNA.

Ano: 2006

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3176

PAB

Costa,Maria Angélica Pereira de Carvalho; Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli.

The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field.

Biotechnology applied to Annona species: a review

Palavras-chave: Stoma; Somatic hybridization; Scanning electron microscopy; Polyploidy.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000300015

Rev. Bras. Frutic.

Encina,Carlos Lopez; Martin,Elisabeth Carmona; Lopez,Antonio Arana; Padilla,Isabel Maria Gonzalez.

Annonaceae is an ancient family of plants including approximately 50 genera growing worldwide in a quite restricted area with specific agroclimatic requirements. Only few species of this family has been cultivated and exploited commercially and most of them belonging to the genus Annona such as A. muricata, A. squamosa, the hybrid A. cherimola x A. squamosa and specially Annona cherimola: the cherimoya, commercially cultivated in Spain, Chile, California, Florida, México, Australia, Ecuador, Peru, Brazil, New Zealand and several countries in South and Central America. The cherimoya shows a high degree of heterozygosis, and to obtain homogeneous and productive orchards it is necessary to avoid the propagation by seeds of this species. Additionally, the...

Microsporogenesis in Brachiaria bovonei (Chiov.) Robyns and B. subulifolia (Mez) Clayton (Poaceae)

Palavras-chave: Annona; In vitro; Micropropagation; Protoplasts; Organogenesis; Polyploidy.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000500002

Scientia Agricola

Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.

Some African species of Brachiaria have been introduced into the Americas and became the most important forage for pastures in the tropics. New cultivars can be obtained either from direct selections from the natural existing variability in the germplasm collections or from interspecific hybridizations. Polyploidy is predominant in the genus Brachiaria and correlated with apomixis which complicates hybridization. The objective of cytological studies underway on the Brachiaria germplasm collection at Embrapa Beef Cattle is to determine the chromosome number and meiotic behavior of accessions. For the breeding of Brachiaria species, compatible sexual and apomictic accessions need to be identified. Microsporogenesis was evaluated in two accessions of...

Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae)

Palavras-chave: Brachiaria; Meiosis; Chromosome number; Polyploidy; Breeding program.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500015

Cordeiro,Joel Maciel Pereira; Felix,Leonardo P..

ABSTRACT Although the chromosome number 2n = 28 predominates in most species of the genus Senna, variations are often observed, resulting from either polyploidy (2n = 42, 56, 112) or disploidy (2n = 22, 24, 26) events. To better understand the karyotypic variations in Senna, we examined heterochromatin patterns of 10 species of that genus using chromomycin A3 (CMA) and 4’6-diamidino-2-phenylindole (DAPI) staining, and reviewed information on the chromosome counts of 72 species of that genus. The CMA/DAPI banding patterns were relatively variable among the 10 species, both in terms of the numbers of bands (from two to 26) and their locations on the chromosomes (terminal or proximal regions). Our review indicated that 2n = 28 is the most common ploidy among...

Self-sterility in the hexaploid Handroanthus serratifolius (Bignoniaceae), the national flower of Brazil

Palavras-chave: Chromosome number; CMA/DAPI; Disploidy; Leguminosae; Polyploidy.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100128

Alves,Mariana Ferreira; Duarte,Mariana Oliveira; Oliveira,Paulo Eugênio; Sampaio,Diana Salles.

Polyploidization is common among angiosperms and might induce typically allogamous plants to become autogamous (self-compatible, relying on sexual self-fertilization) or apomictic (achieving asexual reproduction through seeds). This work aimed to determine whether neopolyploidy leads to the breakdown of the self-incompatibility system in the hexaploid non-apomictic species Handroanthus serratifolius (Vahl) S. Grose, through analyses of its floral biology, pollination biology and breeding system. Although anthesis lasted for three days, increasing the overall floral display, receptivity decreased as of the second day. Centridini and Euglossini bees were the main pollinators, and low nectar availability (1.95 ± 1.91 µl/flower) might have obliged them to...

New chromosome numbers in Pilocarpus Vahl (Rutaceae)

Palavras-chave: Apomixis; Late-acting self-incompatibility; Monoembryony; Polyploidy; Tabebuia serratifolia.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400010

Skorupa,Ladislau A..

Chromosome counts for eight species of Pilocarpus Vahl (Rutaceae) a native of Brazil are reported for the first time. Chromosome numbers were determined from mitotic root tip cells of seedlings derived from field collections and grown in the greenhouse. Feulgen staining was used. Initial pre-treatment of root tips was done by using a saturated aqueous solution of alpha-bromonapthalene for two hours at room temperature (20-25ºC). Chromosome numbers of 2n=44 and 2n=88 were determined for the examined taxa. The present results suggest the occurrence of tetraploidy in P. spicatus St.-Hil. and P. carajaensis Skorupa, and a possible basic number x=22 to the genus Pilocarpus.

Post pollination events in a self-sterile hexaploid monoembryonic lineage of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae)

Palavras-chave: Pilocarpus; Rutaceae; Cusparieae; Cytology; Polyploidy.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062000000100002

Alves,Mariana Ferreira; Bittencourt Júnior,Nelson Sabino; Oliveira,Paulo Eugênio; Sampaio,Diana Salles.

ABSTRACT In diploid late-acting self-incompatible (LSI) species of Bignoniaceae, self-pollinated pistils show a marked delay in ovule penetration by pollen tubes, followed by delayed, but otherwise normal, initial stages of endosperm development and subsequent pistil abscission. Most polyploid species of Bignoniaceae are apomictic and set selfed fruits with viable polyembryonic seeds carrying adventitious embryos. Handroanthus serratifolius is a polyploid species with sporophytic apomictic individuals that produce polyembryonic seeds after either self- or cross-pollination, and other LSI individuals that produce exclusively monoembryionic seeds. Our aim was to determine whether self-sterility events in the latter are similar to those observed in other...

Karyotype characterization and evolution of chromosome number in Cactaceae with special emphasis on subfamily Cactoideae

Palavras-chave: Adventitious embryo precursor cells; Handroanthus serratifolius; Polyploidy; Self-sterility; Tabebuia serratifolia.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000300530

Castro,Juliana P.; Moraes,Ana Paula; Chase,Mark W.; Santos,Angeline M. S.; Batista,Fabiane R. C.; Felix,Leonardo P..

ABSTRACT Cactaceae species are karyotypically well-known with x = 11 and chromosome number variation due mainly to polyploidization. However, both assumptions are based on descriptive observations without taking an evolutionary framework of Cactaceae into account. Aiming to confirm these hypotheses in an evolutionary context, we obtained chromosome numbers for 20 species of Cactoideae, performed an extensive review of chromosome number for the family, and analyzed these data using a phylogenetic approach. The karyotypes presented here were characterized by CMA/DAPI banding, and for six species 5S and 45S rDNA sites were located. Our data, along with a survey of the literature, reinforce the long-standing hypothesis of a x = 11 as the base chromosome number...

First cytomolecular characterization of three Neotropical woody bamboos (Bambusoideae, Poaceae) suggests ancient diploidized karyotypes

Palavras-chave: Base chromosome number; CMA/DAPI; Dysploidy; Heterochromatin; Polyploidy; RDNA.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100135

Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete.

ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was...

Sobrevivência e diferenciação de protocormos de Oncidium flexuosum submetidos a tratamento com ácido peracético e colchicina - DOI: 10.4025/actasciagron.v31i3.697

Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673

Agronomy

Unemoto, Lilian Keiko; UEL; Faria, Ricardo Tadeu de; UEL; Destro, Deonisio; UEL; Barbosa, Cristiane Muniz; UEL; Lone, Alessandro Borini; UEL.

O objetivo deste trabalho foi verificar o efeito do ácido peracético e da colchicina na diferenciação de protocormos de Oncidium flexuosum. O ácido peracético foi testado previamente nas concentrações de 0, 3, 6 e 9 mL L-1, durante dez dias, para verificar a eficácia na esterilização do meio de cultura e os possíveis efeitos fitotóxicos nos protocormos. Após determinação da concentração, foram adicionados 6 mL L-1 de ácido peracético em meio MS com 0,05% de colchicina e DMSO 1%. Os protocormos foram mantidos em agitação pelos períodos: 0, 24, 48, 72, 96 e 120h. O experimento foi inteiramente casualizado com seis tratamentos, dez repetições e cinco protocormos por repetição. Após 30 dias, foi avaliada a sobrevivência dos protocormos e, após 180 dias, foram...

Palavras-chave: 5.01.00.00-9 Agronomia Orchidaceae; Poliploidia; Fitotoxicidade; Esterilização Fitotecnia Orchidaceae; Polyploidy; Phytotoxicity; Sterilization.

Ano: 2009

URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/697

Karyological studies in Brazilian species of Lippia L. (Verbenaceae)

Anais da ABC (AABC)

Sousa,Saulo M.; Torres,Giovana A.; Viccini,Lyderson F..

The genus Lippia (Verbenaceae) comprise around 160 species spread out mainly in South and Central Americas with few African species, some of them with potential medicinal use. Brazil is one the most important centers of diversity with approximately 75% of the species described so far. Innumerous species are endemic and poorly studied especially at a cytological level. Here, chromosomal length, karyomorphology and chromosome asymmetry of twelve Brazilian species of Lippia were evaluated [L. alba (Miller) N.E.Brown, L. diamantinensis Glaz., L. florida Cham., L. hermanioides Cham., L. lacunosa Mart. & Schauer, L. lupulina Cham., L. pohliana Schauer, L. pseudothea (St. Hil) Schauer, L. rosella Moldenke, L. rotundifolia Cham., L. rubella Moldenke and L....

Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho & Hiern) Stapf (Gramineae)

Palavras-chave: Chromosomes; Cytology; Dysploidy; Karyology; Lippia; Polyploidy.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400017

Biocell

Sayuri Utsunomiya,Karina; Suely Paglirini,Maria; Borges do Valle,Cacilda.

The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho & Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x = 36). Chromosomes paired preferentially as bivalents, but quadrivalents were...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300007

Palavras-chave: Brachiaria nigropedata; Chromosome number; Meiosis; Polyploidy; Apomixis; Grasses.

Ano: 2005

Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques

Ciência Rural

Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena.

The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was...