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Registros recuperados: 117
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Population structure and genetic variability of mainland and insular populations of the Neotropical water rat, Nectomys squamipes (Rodentia, Sigmodontinae) Genet. Mol. Biol.
Almeida,Francisca C.; Maroja,Luana S.; Moreira,Miguel A.M.; Seuánez,Héctor N.; Cerqueira,Rui.
Seven microsatellite loci were used to investigate the genetic variability and structure of six mainland and two island populations of the Neotropical water rat Nectomys squamipes, a South American semi-aquatic rodent species with a wide distribution. High levels of variability were found within mainland populations while island populations were less variable but the more differentiated in respect to allele number and frequency. The time of biological divergence between mainland and island populations coincided with geological data. A significant geographic structure was found in mainland populations (theta = 0.099; rho = 0.086) although the degree of differentiation was relatively low in respect to the distance between surveyed localities (24 to 740 km)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Population genetics; Islands; Population expansion; Population size; Divergence time.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500008
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Inclusion of South American samples reveals new population structuring of the blacktip shark (Carcharhinus limbatus) in the western Atlantic Genet. Mol. Biol.
Sodré,Davidson; Rodrigues-Filho,Luis F.S.; Souza,Rosália F.C.; Rêgo,Péricles S.; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcharhinus limbatus; Control region; Fishery stocks; Mitochondrial DNA; Population genetics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500008
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Patterns of genetic diversity in southern and southeastern Araucaria angustifolia (Bert.) O. Kuntze relict populations Genet. Mol. Biol.
Souza,Maria Isabel Ferreira de; Salgueiro,Fabiano; Carnavale-Bottino,Mariana; Félix,Durvalina Benedita; Alves-Ferreira,Marcio; Bittencourt,Juliana Vitoria Messias; Margis,Rogério.
Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucariaceae; Relict populations; Araucaria forest; Population genetics; AFLP.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300018
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Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci Genet. Mol. Biol.
Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de.
A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population genetics; Plant breeding; Molecular markers.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022
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Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae) Genet. Mol. Biol.
Guidugli,Marcela Corbo; Accoroni,Klaus Alvaro Guerrieri; Mestriner,Moacyr Antonio; Contel,Eucleia Primo Betioli; Martinez,Carlos Alberto; Alzate-Marin,Ana Lilia.
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Jequitibá rosa; Population genetics; SSR markers; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100022
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Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia Genet. Mol. Biol.
Berdugo,Gilberto Orozco; Narváez Barandica,Juan C..
Prochilodus magdalenae is an endemic freshwater fish that occurs in the Magdalena, Sinú and Atrato hydrographic basins. It has an important economic role and is a food resource for the artisanal fishing communities. Its socioeconomic importance contrasts with the current status of its fisheries, where stocks are being depleted. Considering its importance and lack of information on its genetic structure, we used seven microsatellite markers to assess the genetic structure of wild populations of P. magdalenae. The genetic diversity was assessed and the population genetic structure was estimated through Fst, analysis of molecular variance and Bayesian analysis. A total of 290 alleles were found in all loci throughout all population. The high polymorphism...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neotropical fish; Microsatellite; Conservation genetics; Population genetics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100008
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AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest Genet. Mol. Biol.
Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M..
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012
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Studies on diversity and evolution of Iridaceae species in southern Brazil Genet. Mol. Biol.
Souza-Chies,Tatiana T.; Santos,Eliane Kaltchuk dos; Eggers,Lilian; Flores,Alice Mainieri; Alves,Eudes M. Stiehl; Fachinetto,Juliana; Lustosa,Juliana; Corrêa,Lauís Brisolara; Tacuatiá,Luana Olinda; Piccoli,Paula; Miz,Rogéria Beatriz.
Plants of the family Iridaceae are well represented in the grassland vegetation of southern Brazil, occurring in the Pampa and Atlantic Forest biomes. Nevertheless, little is known about the taxonomy and evolution of Iridaceae species in southern Brazil. The main goal of this review is to compile published information about South American Iridaceae, and to discuss the evolution and genetic diversity of the family presenting our own research data in the light of the published literature. The main focus is on the genera Calydorea, Cypella, Herbertia, and Sisyrinchium. Aspects of reproductive system and of pollinator attraction are also discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotaxonomy; Molecular phylogenetics; Iridoideae; Population genetics; Diversity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600018
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Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae) from the Brazilian Atlantic Forest Genet. Mol. Biol.
Maroja,L.S.; Almeida,F.C.; Seuánez,H.N..
We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus) and high levels of both observed (0.718-0.789) and expected (0.748-0.832) heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q) and R ST (r) analogues was 0.037 for q and 0.060 for r. There was significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Migration; Population genetics; Schistosoma mansoni host; Sigmodontinae.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400002
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Allozymic variation and differentiation in the chilean blue mussel, Mytilus chilensis, along its natural distribution Genet. Mol. Biol.
Toro,Jorge E.; Castro,Grace C.; Ojeda,Johana A.; Vergara,Ana M..
Genetic differentiation in the Chilean blue mussel Mytilus chilensis (Hupé 1854) was investigated based on the variation in the allozyme frequencies of Pgm, Gpi, Icd, Me, Gsr, Lap and Pep in eight samples collected along 1800 km from Arauco (VIII Region) to Punta Arenas (XII Region). Despite the large geographic separations, values of Neis unbiased genetic distance, D (0.004-0.048) and standardised genetic variation among populations, Fst (0.011-0.055) were small. The levels of gene flow (Nm = 8) found in this study prevent the effect of differentiation among populations by genetic drift. This findings indicate that its long-lived planktotrophic larvae provides this species with considerable dispersal ability throughout its range which is favoured by the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozyme; Mytilus chilensis; Population genetics; Gene flow; Chile.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100031
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Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing Genet. Mol. Biol.
Vaini,Jussara Oliveira; Mota,Kenneth Gabriel; Ojeda,Alejandra Paola; Barreiros,João Pedro; Moreira,Renata Guimarães; Hilsdorf,Alexandre Wagner Silva.
Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Short tandem repeat; Population genetics; Conservation; Fishery; Marine resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100074
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Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil Genet. Mol. Biol.
Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028
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Gene variation and genetic differentiation among populations of the solitary mud dauber wasp Trypoxylon (Trypargilum) albitarse Fabricius 1804 (Hymenoptera, Crabronidae) Genet. Mol. Biol.
Bergamaschi,Antonio C.B.; Lama,Marco A. Del.
Abstract Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to northern Argentina. Eight species-specific microsatellite loci were used for genotyping 96 adult wasps (one female per nest) sampled at five sites in Brazil. The analysis of allelic richness and private alleles indicated high genetic diversity in the populations sampled. Pairwise comparisons using the Fst and Dest indices revealed significant differentiation for all, but one pair of populations. Fst, Dest, AMOVA and assignment test...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population genetics; Wasps; Crabronidae; SSR markers.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400519
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Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata Genet. Mol. Biol.
Jamsari,Amirul Firdaus Jamaluddin; Min-Pau,Tan; Siti-Azizah,Mohd Nor.
Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; Population genetics; Microsatellite; Multiplex PCR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029
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Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history Genet. Mol. Biol.
da Silva Santos,Anelisie; Trigo,Tatiane Campos; de Oliveira,Tadeu Gomes; Silveira,Leandro; Eizirik,Eduardo.
Abstract The pampas cat is a small felid that occurs in open habitats throughout much of South America. Previous studies have revealed intriguing patterns of morphological differentiation and genetic structure among its populations, as well as molecular evidence for hybridization with the closely related L. tigrinus. Here we report phylogeographic analyses encompassing most of its distribution (focusing particularly on Brazilian specimens, which had been poorly sampled in previous studies), using a novel dataset comprising 2,143 bp of the mitogenome, along with previously reported mtDNA sequences. Our data revealed strong population strutucture and supported a west-to-east colonization process in this species’ history. We detected two population expansion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogeography; Population genetics; Mitochondrial DNA; Conservation genetics; Historical demography.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200273
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Genetic characterisation of populations of the critically endangered Goliath grouper ( Epinephelus itajara, Serranidae) from the Northern Brazilian coast through analyses of mtDNA Genet. Mol. Biol.
Silva-Oliveira,Gláucia C.; Rêgo,Péricles S. do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The Goliath grouper ( Epinephelus itajara ) is one of the most endangered species of fish of the subfamily Epinephelinae. Slow to develop and mature, and dependent on mangrove habitats for breeding, the species also suffers intense harvesting, which has reduced drastically in numbers in many areas. To contribute to the understanding of the characteristics of E. itajara populations, we conducted a molecular genetics study of the species, focusing on populations from the Northern Brazilian coast. The mtDNA control region (D-loop) of 116 individuals from five localities (Bragança, Ajuruteua, Parnaíba, Fortaleza and Natal) was analysed, and a sequence of 499 base pairs identified. Analyses of the sequences indicated that genetic variability was generally...
Palavras-chave: Goliath grouper; Epinephelus itajara; Control region; MtDNA; Population genetics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500029
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Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae) Genet. Mol. Biol.
Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013
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Lack of functional KL-VS polymorphism of the KLOTHO gene in the Korean population Genet. Mol. Biol.
Kim,Hee-Kwon; Jeong,Byung-Hoon.
Abstract The functional variant of the Klotho "KL-VS" stretch, which includes six polymorphisms in linkage disequilibrium, is reportedly associated with healthy aging and longevity in European and American populations. Among Asian populations, this variant has been observed in the Indian population but not in the Iranian population. An association between KL-VS polymorphism and aging has not been reported in Koreans. To investigate whether the KL-VS polymorphism could be associated with healthy aging and longevity in a Korean population, we analyzed genotype and allele frequencies of the KL-VS variant in a large Korean population sample. The KL-VS variant was not found in 874 Korean individuals. Thus, it is not possible to test its association to aging in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Aging; KLOTHO gene; KL-VS polymorphism; Population genetics.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300370
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Short-tandem repeat analysis in seven Chinese regional populations Genet. Mol. Biol.
Song,Xing-bo; Zhou,Yi; Ying,Bin-wu; Wang,Lan-lan; Li,Yi-song; Liu,Jian-feng; Bai,Xiao-gang; Zhang,Lei; Lu,Xiao-jun; Wang,Jun; Ye,Yuan-xin.
In the present study, we investigated the application of 13 short tandem repeat (STR) loci (D13S317, D7S820, TH01, D16S539, CSFIPO, VWA, D8S1179, TPOX, FGA, D3S1358, D21S11, D18S51 and D5S818) routinely used in forensic analysis, for delineating population relationships among seven human populations representing the two major geographic groups, namely the southern and northern Chinese. The resulting single topology revealed pronounced geographic and population partitioning, consistent with the differences in geographic location, languages and eating habits. These findings suggest that forensic STR loci might be particularly powerful tools in providing the necessary fine resolution for reconstructing recent human evolutionary history.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forensic medicine; Population genetics; Short-tandem repeat; Human evolutionary history; Genetic distance.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400002
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Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations Genet. Mol. Biol.
Tavares,Mara Garcia; Pietrani,Nathalia Teixeira; Durvale,Maxwell de Castro; Resende,Helder Canto; Campos,Lucio Antonio de Oliveira.
Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic differentiation; Microsatellites; Population genetics; Stingless bees.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016
Registros recuperados: 117
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