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| Registros recuperados: 42 | |
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| Motter,MM; Corva,P; Krause,M; Perez Cenci,M; Soria,L.. |
| La calpastatina es una enzima inhibidora de las calpaínas, principales enzimas proteolíticas del músculo esquelético. Está codificada por el gen CAST, a partir del cual se pueden expresar cuatro isoformas proteicas diferentes debido a la existencia de cuatro promotores distintos. Este gen ha sido estudiado como candidato para explicar diferencias de origen genético en la terneza de la carne. En bovinos se han descripto varios polimorfismos del tipo SNP en distintas regiones del gen. Algunos de ellos han sido incluidos en test comerciales por su asociación significativa con la variabilidad en terneza. Recientemente, la investigación se ha focalizado en establecer qué promotores están activos en músculo esquelético y si los mismos presentan polimorfismos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calpastatina; Gen CAST; Bovinos; Terneza; SNP. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332009000100003 |
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| Bailliet,Graciela; Ramallo,Virginia; Muzzio,Marina; Santos,María R; Motti,Josefina MB; Bianchi,Néstor O; Bravi,Claudio M. |
| Los avances en el conocimiento del genoma humano han llegado también a una situación comprensiva en relación a la región específica del cromosoma Y humano. En los últimos años se han reconocido al menos 600 polimorfismos bialélicos (SNP), a partir de los cuales se ha construido una sólida filogenia y se ha normalizado la nomenclatura. En el presente trabajo se han estudiado 940 muestras de varones no relacionados de 10 poblaciones aborígenes de Argentina, Chile y Paraguay; y 12 poblaciones urbanas de Argentina. Se han analizado 9 marcadores bialélicos para reconocer los clados mayores de la filogenia. Fue posible distinguir los haplogrupos Q* y Q3 autóctonos, y los haplogrupos AB, DE, F, K, P y R alóctonos. El Fst fue de 0, 17, mostrando un alto nivel de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Linajes paternos; Haplogrupos; SNP; Ancestralidad genética; Poblaciones humanas. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000100003 |
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| Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de. |
| Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121 |
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| Petrović,Gordana; Nikolić,Zorica; Đorđević,Vuk; Župunski,Vesna; Jovičić,Dušica; Ignjatov,Maja; Milošević,Dragana. |
| O objetivo deste trabalho foi avaliar a variação genética do inibidor de tripsina em variedades cultivadas (Glycine max) e silvestres (Glycine soja) de soja. Foram avaliadas as variações genéticas do inibidor de tripsina Kunitz, representado pela proteína 21-kDa (KTI), e do inibidor de tripsina-quimotripsina Bowman-Birk (BBI), em variedades de soja cultivadas (G. max) e selvagens (G. soja). Ensaios de clivagem foram feitos com endonuclease de incompatibilidade heteroduplex, para a detectar mutações no gene de KTI, com uma única nuclease específica de cadeia simples, obtida a partir de extractos de aipo (CEL I). As variedades de soja estudadas apresentaram baixo nível de variação genética em KTI e BBI. A análise por PCR -RFLP dividiu o BBI-A em A1 e A2 e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Glycine soja; Factores antinutritionais; Inibidores de protease; SNP. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000200102 |
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| Zhang,XX; Ran,JS; Lian,T; Li,ZQ; Yang,CW; Jiang,XS; Du,HR; Cui,ZF; Liu,YP. |
| ABSTRACT Myostatin (MSTN) is a negative regulator of skeletal muscle growth. In order to investigate whether there is a correlation between MSTN polymorphisms and chicken production performance, in this study, single nucleotide polymorphisms (SNPs) in MSTN gene were examined across 180 Daheng broilers by direct sequencing of PCR product, and the correlations between the genotype and body weight at the age of 1-10 weeks and carcass traits at the age of 73 day were analyzed. Five SNPs (rs313622770, rs313744840, rs316247861, rs314431084, rs317126751) of MSTN gene were identified across Daheng broiler samples, and four haplotypes were reconstructed based on the five SNPs. Results of association analysis showed that four (rs313622770, rs313744840, rs316247861... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Association analysis; Chicken; Production performance; MSTN; SNP. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300319 |
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| Bai,J; Dong,ZH; Lei,Y; Yang,YB; Jia,XP; Li,JY. |
| ABSTRACT This study investigated SNP mutation sites of Gonadotrophin releasing hormone (GnRH) gene in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologies. Moreover, polymorphism of GnRH gene and its association with growth traits of quail were analyzed, aiming to get molecular markers associated to growth traits of quail, which could provide references for breeding of new quail species. According to research results, a total of 14 SNP mutation sites of GnRH were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. Except that only 2 genotypes were detected for A209C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gonadotrophin releasing hormone (GnRH) gene; DNA sequencing; SNP; Polymorphism; Body weight. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100312 |
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| Bai,Jun Yan; Wang,Xu; Yang,You Bing; Zhang,Xiao Hui; Pang,You Zhi; Li,Hong Wei. |
| ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep), using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP) loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T), C71G (C/G), and C330G (C/G). C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA pooling; Phylogenetic tree; Sheep; SNP. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001000604 |
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| Rekaya,Romdhane; Robbins,Kelly. |
| In recent years there has been much focus on the use of single nucleotide polymorphism (SNP) fine genome mapping to identify causative mutations for traits of interest; however, many studies focus only on the marginal effects of markers, ignoring potential gene interactions. Simulation studies have show that this approach may not be powerful enough to detect important loci when gene interactions are present. While several studies have examined potential gene interaction, they tend to focus on a small number of SNP markers. Given the prohibitive computation cost of modeling interactions in studies involving a large number SNP, methods need to be develop that can account for potential gene interactions in a computationally efficient manner. This study adopts... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genome; Simulation; SNP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300011 |
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| Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira. |
| The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004 |
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| Mello,Fernanda de; Guimarães,Marta Fonseca Martins; Cobuci,Jaime Araújo; Silva,Marcos Vinícius Gualberto Barbosa da; Braccini Neto,José; Paiva,Daisyléa de Souza. |
| Objetivou-se obter os índices de diversidade genética para o SNP (single nucleotide polymorphism) do íntron 4 do gene da osteopontina (OPN) para 434 animais (87 touros e 347 vacas) participantes do Teste de Progênie da raça girolando no Brasil. Para a amplificação, foram utilizados primers descritos para a raça holandesa, e a diferenciação dos alelos C/T desse SNP foi obtida por meio da técnica de PCR-RFLP. As frequências genotípicas TT (52,53%), CT (38,71%) e CC (8,76%) e as frequências alélicas de T (71,9%) e C (28,1%) indicam que a população encontra-se em Equilíbrio de Hardy-Weinberg (EHW). Apesar de o loco do gene OPN estar em EHW, a frequência superior do alelo T do SNP nesses animais pode sugerir uma tendência de fixação do alelo T na raça. Não foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovinos leiteiros; Polimorfismo; Produção de leite; SNP; SPP1. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001100013 |
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| Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane. |
| ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311 |
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| Silva,F.F.; Rocha,G.S.; Resende,M.D.V.; Guimarães,S.E.F.; Peternelli,L.A.; Duarte,D.A.S.; Azevedo,C.. |
| Foi proposta uma metodologia para avaliação genética de curvas de crescimento considerando-se informações de marcadores SNPs (Single Nucleotide Polymorphisms). Em um primeiro passo foram ajustados modelos de crescimento não lineares (logístico) aos dados de peso-idade de cada animal, e em um segundo passo as estimativas dos parâmetros de tais modelos foram consideradas como fenótipos em um modelo de regressão (LASSO Bayesiano - BL) cujas covariáveis foram os genótipos dos marcadores SNPs. Este enfoque possibilitou estimar os valores genéticos genômicos (GBV) para peso em qualquer tempo da trajetória de crescimento, refletindo na confecção de curvas de crescimento genômicas, as quais permitiram a identificação de grupos de indivíduos geneticamente... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SNP; LASSO bayesiano; Dados longitudinais. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500033 |
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| Prioli,R.A.; Curi,R.A.; Chardulo,L.A.; Gomes,V.N.; Prioli,S.M.A.P.; Mota,M.D.S.. |
| The objectives of this study were to standardize a PCR-RFLP genotyping method for the AY_731081:g.1900T>C SNP of the equine CD14 gene, and to characterize this SNP and two other polymorphisms (AY_005808: c.1530A>G of the TLR4 gene and AX_463789: g.133T>C of the Cε gene) in Mangalarga horses, in order to contribute to future studies investigating the association between DNA markers and traits related to immune system physiology in this breed. A total of 151 Mangalarga horses of both sexes and variable ages, representative of the population of São Paulo State, were used. PCR-RFLP was found to be adequate for genotyping of the AY_731081: g.1900T>C SNP of the equine CD14 gene. However, this polymorphism is probably not present in Mangalarga horses,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; CD14; Cε; SNP; TLR4. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500030 |
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| Yuan,Jun; Zhang,Yan; Yan,Fu-tang; Zheng,Xiao. |
| ABSTRACT Objective: Studies have indicated that AMPK play critical roles in the regulation of innate immunity and inflammatory responses. However, the role of the polymorphisms of PRKAA1 gene in immune-response to infectious organisms remains unknown. To evaluate the potential role of PRKAA1/AMPKα1 in the immune-response to HBV, we conducted this case-control study. Methods: We recruited 276 patients (145 men and 131 women; average age, 51.6 years) with chronic HBV infection (CHB) and 303 healthy controls (166 men and 137 women; average age, 54.2 years). All the subjects were unrelated individuals of Chinese Han Population. Three SNPs of PRKAA1gene were tested. Results: Rs1002424 polymorphism showed significant difference in the allele frequencies, but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HBV; PRKAA1 gene; SNP; Association study. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600564 |
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| Tian,Y.G.; Yue,M.; Gu,Y.; Gu,W.W.; Wang,Y.J.. |
| Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Minipigs; SNP; GH; GHR; IGF-1. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900753 |
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| Registros recuperados: 42 | |
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