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	Provedor de dados Genet. Mol. Biol. (18) Electron. J. Biotechnol. (11) PAB (11) Scientia Agricola (5) Rev. Bras. Frutic. (5) Colegio de Postgraduados (4) BABT (3) Ciência Rural (3) Horticultura Brasileira (3) Neotropical Ichthyology (3) Acta Agron. (Palmira) (2) Ciencia e Investigación Agraria (2) Infoteca-e (2) Acta Amazonica (2) Ciência e Agrotecnologia (2) Planta Daninha (2) Thai Agricultural (1) Chilean J. Agric. Res. (1) OAK (1) Acta Botanica (1) Mais... Autor Souza,Anete Pereira de (4) Hinrichsen,Patricio (3) Schuster,Ivan (3) Vencovsky,Roland (3) Aguirre Robert, Cinthia Jimena (2) Benchimol,Luciana Lasry (2) Borges,Aline (2) Bressan,Eduardo de Andrade (2) Campos,Tatiana de (2) Carbonell,Sérgio Augusto Moraes (2) Chioratto,Alisson Fernando (2) Coelho,Alexandre Siqueira Guedes (2) Colombo,Carlos Augusto (2) Contreras Toledo, Aremi Rebeca (2) Ferreira,Dhiego G. (2) Formighieri,Eduardo Fernandes (2) Galindo,Bruno A. (2) Guimarães,Paula de Souza (2) Laborda,Prianda Rios (2) Lüders,Reginaldo Roberto (2) Mais... Palavra-chave SSR (87) Genetic diversity (12) Molecular markers (8) AFLP (7) Glycine max (7) Genetic variability (5) Polymorphism (5) RAPD (4) Fingerprinting (3) Genetic structure (3) Maestría (3) Marcador molecular (3) Marcadores moleculares (3) Phaseolus vulgaris (3) Polimorfismo (3) QTL (3) Accesiones (2) Capsicum annuum (2) Caracteres morfológicos (2) Conservation (2) Mais... Tipo do documento Info:eu-repo/semantics/article (62) Journal article (14) Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) (2) Info:eu-repo/semantics/other (2) Tesis (2) Info:eu-repo/semantics/report (1) Mais... Ano 2022 (1) 2020 (6) 2019 (4) 2018 (3) 2016 (2) 2015 (4) 2014 (1) 2013 (5) 2012 (10) 2011 (8) 2010 (11) 2009 (7) 2008 (9) 2007 (7) 2006 (3) 2005 (1) 2004 (3) 2003 (2) Mais... País Brazil (65) Chile (14) Mexico (4) Colombia (2) Japan (1) Thailand (1) Mais... Idioma Inglês (60) Português (19) Espanhol (6) Japonês (1) Thailandês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 87 Primeira ... 1 2 3 4 5 ... Última The genetic structure and mating system of Acrocomia aculeata (Arecaceae) Genet. Mol. Biol. Abreu,Aluana Gonçalves; Priolli,Regina Helena Geribello; Azevedo-Filho,Joaquim Adelino; Nucci,Stella Maris; Zucchi,Maria Imaculada; Coelho,Ricardo Marques; Colombo,Carlos Augusto. Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from São Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed... Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; Mating system. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100016 Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean Genet. Mol. Biol. Belarmino,Luis C.; Oliveira,Ana R. da S.; Brasileiro-Vida,Ana C.; Bortoleti,Kyria C. de A.; Bezerra-Neto,João Pacífico; Abdelnoor,Ricardo V.; Benko-Iseppon,Ana M.. Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene families; FISH; BAC; SSR; Aquaporin; Bioinformatics. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200015 A novel statistical method for assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.) Genet. Mol. Biol. Liu,Zhi-Peng; Liu,Gong-She; Yang,Qing-Chuan. The level of variation of simple sequence repeat (SSR) markers in cultivated alfalfa from American, Australian and Chinese sources was evaluated using a novel autotetraploid statistical method to calculate the effective number of alleles, the allele frequencies and heterozygosity. We used 19 SSR primers to screen seven polymorphic SSR loci in 320 plants from eight populations. The genetic distance and phylogenetic analysis (DISPAN) program was used to calculate the inter- and intra-population genetic relationships using the conventional binary absence/presence (0/1) method and our novel autotetraploid method. The autotetraploid method resulted in significantly higher heterozygosity (p < 0.01), average effective number (p < 0.01) and lower standard... Tipo: Info:eu-repo/semantics/article Palavras-chave: Autotetraploid; Genetic variation; Medicago sativa L.; SSR; Statistical genetics. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300015 Development of microsatellite markers for identifying Brazilian Coffea arabica varieties Genet. Mol. Biol. Vieira,Elisa S.N.; Von Pinho,Édila V. de R.; Carvalho,Maria G.G.; Esselink,Danny G.; Vosman,Ben. Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of... Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; Coffee; Genetic similarity; Molecular marker. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022 Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus) Genet. Mol. Biol. Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann. Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare. Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015 Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus Genet. Mol. Biol. Ceresini,Paulo Cezar; Silva,Cristina Lacerda Soares Petrarolha; Missio,Robson Fernando; Souza,Elaine Costa; Fischer,Carlos Norberto; Guillherme,Ivan Rizzo; Gregorio,Ivo; Silva,Eloiza Helena Tajara da; Cicarelli,Regina Maria Barreto; Silva,Marco Túlio Alves da; Garcia,José Fernando; Avelar,Gustavo Arbex; Porto Neto,Laercio Ribeiro; Marçon,André Ricardo; Bacci Junior,Maurício; Marini,Danyelle Cristine. The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinformatics tool; Genetic markers; Gene ontology; Small sequence repeats; SSR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400014 Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers Genet. Mol. Biol. Zucchi,Maria Imaculada; Brondani,Rosana Pereira Vianello; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland. The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including... Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; Myrtaceae; Cerrado; Tropical tree; Genetic diversity and transferability. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008 High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae) Genet. Mol. Biol. Tarazi,Roberto; Moreno,Maria Andréia; Gandara,Flávio Bertin; Ferraz,Elza Martins; Moraes,Mário Luiz Teixeira; Vinson,Christina Cleo; Ciampi,Ana Yamaguishi; Vencovsky,Roland; Kageyama,Paulo Yoshio. Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dipteryx alata; Genetic structure; Mating system; SSR. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100015 Genetic diversity in populations of African mahogany (Khaya grandioliola C. DC.) introduced in Brazil Genet. Mol. Biol. Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro. Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (>15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301 Intraspecific differentiation of Chilean isolates of the entomopathogenic fungi Metarhizium anisopliae var. anisopliae as revealed by RAPD, SSR and ITS markers Genet. Mol. Biol. Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge. The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017 Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae) Genet. Mol. Biol. Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del. We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010 Chromosomal localization of microsatellite loci in Drosophila mediopunctata Genet. Mol. Biol. Cavasini,Renato; Batista,Marcos Roberto Dias; Klaczko,Louis Bernard. Drosophila mediopunctata has been used as a model organism for genetics and evolutionary studies in the last three decades. A linkage map with 48 microsatellite loci recently published for this species showed five syntenic groups, which had their homology determined to Drosophila melanogaster chromosomes. Then, by inference, each of the groups was associated with one of the five major chromosomes of D. mediopunctata. Our objective was to carry out a genetic (chromosomal) analysis to increase the number of available loci with known chromosomal location. We made a simultaneous analysis of visible mutant phenotypes and microsatellite genotypes in a backcross of a standard strain and a mutant strain, which had each major autosome marked. Hence, we could... Tipo: Info:eu-repo/semantics/report Palavras-chave: Tripunctata; SSR; Genetic chromosomal analysis; BLAST search; Muller’s elements. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100055 Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers Genet. Mol. Biol. Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland. Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016 Molecular characterization of watermelon cultivars using microsatellite markers Horticultura Brasileira Gama,Renata Natália CS; Santos,Carlos Antônio F; Dias,Rita de Cássia S; Souza,Flávio F. Allelic patterns and genetic similarity among 17 watermelon cultivars were established using microsatellite markers. For visualization of the genetic similarity, the dendrogram UPGMA (Unweighted Pair Group Method with Arithmetic Means) was generated by the similarity matrix of the Jacquard coefficient, based on 34 alleles of ten microsatellite loci. Total DNA was extracted by the CTAB 2x method and PCR (Polymerase Chain Reaction) products were analyzed in denaturing polyacrylamide 6% gels, stained with silver nitrate. The number of base pairs was estimated by the method of inverse mobility, based on known size product regression. Similarity ranged from 34 to 100%, reflecting high genetic variability. Analyzed loci were not enough to distinguish all 17... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrullus lanatus; Genetic divergence; SSR; Plant variety protection. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000400003 DNA fingerprinting of water yam (Dioscorea alata) cultivars in Brazil based on microsatellite markers Horticultura Brasileira Siqueira,Marcos VBM; Dequigiovanni,Gabriel; Corazon-Guivin,Mike A; Feltran,José C; Veasey,Elizabeth A. This study aimed to fingerprint 36 water yam (Dioscorea alata) accessions using microsatellite markers. Ten accessions were collected in local markets from several municipalities in Brazil, eight were obtained from the 'Instituto Agronômico de Campinas' (IAC) germplasm collection and eighteen were collected directly from growers from São Paulo state. A total of nine microsatellite loci were used in the analysis. Loci revealed high polymorphism verified by elevated PIC values (0.57-0.77), and by high gene diversity and Shannon-Wiener indices (0.69 and 1.29 on average, respectively). The accessions were classified into two groups based on clustering analysis. One group contained mostly accessions from the IAC collection, including a commercial cultivar... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dioscorea alata; Commercial varieties; Genetic diversity; Germplasm collection; SSR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000400015 Characterization of pepper accessions using molecular markers linked to pungency and SSR Horticultura Brasileira Jesus,Rafaela de; Santos,Gabriel do N; Piccin,Andressa S; Balsalobre,Thiago WA; Sala,Fernando C; Carneiro,Monalisa S. ABSTRACT Peppers of the genus Capsicum are of great socioeconomic importance, being pungency trait their main attraction. Pungency characterization, genetic distance estimates and population structure analysis of the accessions belonging to germplasm banks are important for parent selection which allows to obtain superior progenies. Therefore, the aims of this study were: i) evaluate 81 accessions of the Capsicum spp. Germplasm Bank of Universidade Federal de São Carlos (BGC-UFSCar) with molecular markers linked to pungency; ii) estimate the genetic diversity among accessions of the BGC-UFSCar using microsatellite markers (SSR); and iii) evaluate the efficiency of these markers in the distinction among species of Capsicum spp. We noticed that pun11 and SNP... Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsicum spp.; SSR; Germplasm; Dissimilarity; Polimorphism. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200152 Estrutura genética de populações silvestres de Oryza glumaepatula em três biomas brasileiros utilizando marcadores microssatélites. Infoteca-e BRONDANI, R. P. V.; RANGEL, P. H. N.; ZUCCHI, M. I.; MAGALHÃES, M. R.; BORBA, T. C. O.; VENCOVSKY, R.; BRONDANI, C.. Oryza glumaepatula, uma das 21 espécies silvestres do gênero Oryza, no qual está incluído o arroz cultivado (O. sativa), constitui uma valiosa fonte doadora de alelos favoráveis para características de interesse agronômico desta espécie. Diversas áreas de ocorrência natural de O. glumaepatula estão sob ameaça de devastação e, desta forma, a implementação de estratégias de conservação destes recursos genéticos a partir de estudos de genética de populações é de extrema relevância. Marcadores moleculares microssatélites, ou SSR, são uma ferramenta útil para investigar questões relacionadas ao sistema de cruzamento, fluxo gênico e estrutura genética das populações naturais de O. glumaepatula. Este trabalho objetivou caracterizar a variabilidade genética e... Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Oryza glumaepatula; Silvestre; SSR; Variabilidade genética.; Arroz; Conservação; Marcador Molecular.. Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/212641 Mapeamento e validação de marcadores microssatélites associados à restauração da fertilidade em sorgo. Infoteca-e PINTO, M. de O.; SANTOS, C. V. dos; MENEZES, C. B. de; PARRELLA, R. A. da C.; SCHAFFERT, R. E.; MAGALHAES, J. V.. Os locos Rf1, Rf2 e Rf5 são responsáveis pela restauração da fertilidade em genótipos de sorgo em citoplasma A1. O objetivo desse trabalho foi mapear e validar marcadores moleculares associados com a restauração da fertilidade em genótipos-elite de sorgo do programa de melhoramento de sorgo sacarino da Embrapa Milho e Sorgo. Foram avaliados 37 locos microssatélites (Simple Sequence Repeats, SSR) na região cromossômica dos locos Rf. Para o mapeamento foram utilizados 166 progênies F2 (CMSXS222B x BR505R) e para a validação foram utilizadas 90 famílias F2:4 (CMSXS157B x BR505R). Apenas os marcadores localizados em uma janela de 47,4 cM, a qual inclui o loco Rf1, apresentaram associação com a restauração da fertilidade na população F2 (CMSXS222B x BR505R),... Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Locos Rf; SSR; Mapeamento genético; Seleção assistida; Sorghum bicolor; Marcador molecular; Melhoramento. Ano: 2014 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1010346 Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification Neotropical Ichthyology Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena. Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers... Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581 Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species Neotropical Ichthyology Apolinário-Silva,Caroline; Ferreira,Dhiego G.; Cavenagh,Analiza F.; Aprígio,Nícollas G. O.; Galindo,Bruno A.; Carlsson,Jens; Sofia,Silvia H.. ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species. Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Lambarizinho; Molecular markers; Neotropical fish; SSR. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100218 Registros recuperados: 87 Primeira ... 1 2 3 4 5 ... Última

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