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Two-graph receiver operating characteristic AgEcon
Reichenheim, Michael E..
The command roctg allows visualizing sensitivity (Se) and specificity (Sp) curves according to the range of values of a new diagnostic test, given a “true” state of an event, the reference test. On request, several options for displaying Se and Sp estimates in, or enhancements for, the graphs are also available.
Tipo: Journal Article Palavras-chave: Concurrent validity; Sensitivity; Specificity; ROC analysis; Research Methods/ Statistical Methods.
Ano: 2002 URL: http://purl.umn.edu/116021
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A mezőgazdasági területek érzékenységének és adaptációs képességének mérési lehetőségei AgEcon
Farkas-Fekete, Maria.
A tanulmány alapvető célja az, hogy hozzájáruljon az érzékenység, sérülékenység és adaptációs képesség vizsgálatának módszertanához. A kutatások eredményei felhívják a figyelmet arra, hogy bár az európai vidék összessége egyre sérülékenyebbé válik a különböző globális kihívásokra, a veszélyeztetettség mértéke régiónként eltérő. A kutatások eredményei megerősítették az IPCC 3. jelentésében megfogalmazott hipotéziseket, melyek szerint a gazdag országok ugyanolyan veszélyeztetettség mellett is kevésbé sérülékenyek, mivel nagyobb az alkalmazkodóképességük. A klímaváltozás, amely pozitív és negatív hatást is gyakorol Európa mezőgazdaságára, módosítja az eddigi komparatív előnyöket, illetve hátrányokat. Gazdasági szempontból Dél- és Közép-Kelet-Európa minden...
Tipo: Journal Article Palavras-chave: Fenntarthatóság; Klímaváltozás; Érzékenység; Sérülékenység; Veszélyeztetettség; Adaptáció; Sustainability; Climate change; Vulnerability; Sensitivity; Adaptability; Agricultural and Food Policy; Environmental Economics and Policy.
Ano: 2009 URL: http://purl.umn.edu/92481
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Estimation of sensitivity and specificity arising from validity studies with incomplete designs AgEcon
Reichenheim, Michael E.; Ponce de Leon, Antonio.
This insert introduces valides, validesi, and validesu, a set of commands that allow the calculations of point and precision estimates of sensitivity and specificity obtained in validity studies with incomplete designs.
Tipo: Journal Article Palavras-chave: Validity; Sensitivity; Specificity; Incomplete design; Research Methods/ Statistical Methods.
Ano: 2002 URL: http://purl.umn.edu/116004
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Initial identification and sensitivity to antimicrobial agents of Salmonella sp.isolated from poultry products in the state of Ceara, Brazil Rev. Bras. Ciênc. Avic.
Oliveira,WF; Cardoso,WM; Salles,RPR; Romão,JM; Teixeira,RSC; Câmara,SR; Siqueira,AA; Marques,LCL.
The objective of this research was to isolate and to verify the sensitivity to antimicrobial agents of strains of Salmonella sp. isolated from poultry products in the state of Ceara, Brazil. A total number of 114 samples was collected from 63 broiler carcasses derived from two processing plants and two supermarkets, and 51 excreta samples were collected in broiler farms located in the state of Ceara, which used three live production stages. Each excreta sample consisted of a fresh excreta pool from 100 birds. Samples were submitted to microbiological analyses, and the isolated Salmonella strains were tested for antimicrobial sensitivity. No Salmonella was isolated from excreta samples, while broiler carcass samples showed a high contamination rate of11.8%....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella sp; Identification; Sensitivity; Poultry products.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300010
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Desiccation tolerance of Tapirira obtusa seeds collected from different environments Rev. bras. sementes
Pereira,Wilson Vicente Souza; Faria,José Márcio Rocha; Tonetti,Olivia Alvina Oliveira; Silva,Edvaldo Aparecido Amaral da.
This study was aimed at evaluating the desiccation sensitivity in seeds of the tree Tapirira obtusa (Benth.) J. D. Mitchell collected from three different environments and subjected to two distinct drying speeds. Seeds were collected from a rocky area, in the "Cerrado", and in a riparian forest area, in the region of municipality of Lavras, State of Minas Gerais. The seeds were subjected to drying with magnesium chloride (slow drying) or silica gel (fast drying), into closed environment, until moisture contents of 40%, 30%, 20% and 10%, considering as control, the percentage of germination at the initial moisture content in each environment, which varied from 47% to 50%. Percentages of germination and normal seedlings as well as germination speed index...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drying; Germination; Sensitivity; Dehydration.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000300005
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การลดเชื้อ Campylobacter jejuni ในซากไก่ด้วยคลอรีนและโอโซน Thai Agricultural
Panrapee Iamtaeweejarloen; Pravate Tuitemwong; Jirawat Kantakrienwong; Kooranee Tuitemwong.
Objectives of this study were to investigate the process sensitivity to Campylobacter spp. and to assess the reduction efficiency of ozonation and chlorination in chilling step of the poultry process using data derived from mCCDA plating and SimPlate MPN methods. Samples of 80 were collected from 4 process steps in the processing plant (scalding, plucking, inside outside washing, and chilling). Numbers of Campylobacter spp. on mCCDA at the respective steps were 3.35, 3.20, 2.68 and 0.20 logCFU/ml, while the SimPlate MPN had 2.54, 2.48, 2.31 and 0.84 log CFU/ml, respectively. Chilling is the most sensitive process to reduce the number of bacteria on both media. Chlorination at 1.5 ppm reduced Campylobacter spp. by 97.46% and 58.62% on mCCDA and SimPlate...
Tipo: Collection Palavras-chave: Poultry process; Ozonation; Campylobacter spp.; MPN; Sensitivity; Chlorination; MCCDA; SimPlate; ไก่สด; การผลิตเนื้อไก่; คลอรีน; โอโซน; การลดเชื้อแบคทีเรีย; ความไว; การลดอุณหภูมิซาก.
Ano: 2010 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5234
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Isolation of Salmonella enterica subsp. enterica (O:4,5:i) and Salmonella enterica subsp. Typhimurium from free-living domestic pigeons (Columba livia) Arq. Bras. Med. Vet. Zootec.
Rocha-e-Silva,R.C.; Cardoso,W.M.; Teixeira,R.S.C.; Albuquerque,Á.H.; Horn,R.V.; Lopes,E.S.; Gomes Filho,V.J.R.; Almeida,C.P.; Santos,I.C.L.; Machado,D.N.; Lima,S.V.G.; Carneiro,I.S..
The present study reports the isolation of Salmonellaenterica in organs of free-living domestic pigeons. In the clinic examination, the presence of feces in the peri-cloacal and abdominal regions were observed, as well as symptoms such as cachexy, incoordination and opisthotonos. Before any therapeutic protocol was applied the bird died and a necropsy was then performed for the removal of spleen, liver, kidney and intestine for bacteriological examination and antibiotic sensitivity test. Salmonella enterica subsp.enterica (O:4,5:i-) and Salmonellaenterica subsp. enterica serovar Typhimurium were isolated from the liver and intestine and the sensitivity test demonstrated that these strains are sensitive to several antibiotics.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Necropsy; Bacteriological examination; Sensitivity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501435
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Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR BABT
Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao.
ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314
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Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A>T polymorphism BJID
Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis.
ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202
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Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes BJMBR
Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I..
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008
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Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México BJM
Aguilar-Barragan,Alejandra; García-Torres,Ana Elisa; Odriozola-Casas,Olga; Macedo-Raygoza,Gloria; Ogura,Tetsuya; Manzo-Sánchez,Gilberto; James,Andrew C.; Islas-Flores,Ignacio; Beltrán-García,Miguel J..
The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fungicide management programs: Rustic management (RM) without applications and Intensive management (IM) more than 25 fungicide application/year. Results showed a decreased sensitivity to all fungicides in isolates collected from IM. Means of EC50 values in mg L-1 for RM and IM were: 13.25 ± 18.24 and 51.58 ± 46.14 for azoxystrobin, 81.40 ± 56.50 and 1.8575 ± 2.11 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banana; Fungicides; Mycosphaerella fijiensis; Sensitivity; Sigatoka.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100051
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Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite BJM
Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310
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Zika detection: comparison of methodologies BJM
Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda.
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zika virus; Detection; Sensitivity; Specificity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144
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Antifungal activity of lectins against yeast of vaginal secretion BJM
Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD BJM
Leal,C.A.G.; Carvalho-Castro,G.A.; Cottorello,A.C.; Leite,R.C.; Figueiredo,H.C.P..
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clinical samples; Sensitivity; Real-time PCR; WSSV; WSD.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
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Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan BJM
Khan,Anwar Sheed; Ali,Sajid; Khan,Muhammad Tahir; Ahmed,Sajjad; Khattak,Yasir; Abduljabbar,; Irfan,Muhammad; Sajjad,Wasim.
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GeneXpert; LED-FM microscopy; Sensitivity; Specificity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400909
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Variation of water potential and trunk diameter answer as sensitivity to the water availability in table grapes Chilean J. Agric. Res.
Silva-Contreras,Claudio; Sellés-Von Schouwen,Gabriel; Ferreyra-Espada,Raul; Silva-Robledo,Herman.
Variation in trunk diameter (TDV) has been proposed as an indicator of the water status of grape vines, with apparently contradictory results. In Vitis vinifera L. var. Crimson Seedless we evaluated the water potential (&#936;), an indicator normally used to determine the water status of grapes, and TDV, comparing two irrigation treatments which restored 100% or 50% of the ETc, to compare the sensitivity of these two indicators to a decrease in soil water. Two evaluation periods of 23 d each were used, the first during the exponential growth phase and the second in the post-veraison period, when the trunk stops growing. In both periods TDV showed coefficients of variation greater than 25%, compared to the 7-10% recorded for water potential. However,...
Tipo: Journal article Palavras-chave: Water potential; Variable displacement sensor; Sensitivity; Itis vinifera.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400001
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Evaluation of the performance of different plastics used to seal nylon cDNA arrays Ciência e Agrotecnologia
Costa Netto,Antônio Paulino da; Drummond,Rodrigo Duarte; Felix,Juliana de Maria; Jorge,Renato Atílio; Menossi,Marcelo.
cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal detection; Nylon arrays; Sensitivity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700031
Registros recuperados: 38
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