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| Registros recuperados: 57 | |
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| Álvarez,Elba; Iglesia,Aleika. |
| Information about the characterization and diagnostic of wild fire was compiled. This bacterial disease, exotic for Cuba, is caused by Pseudomonas syringae pv. tabaci. The species Pseudomonas syringae is traditionally divided into pathovars depending on the host range and symptoms. For its study, different phenotypic and genotypic methods are used. Such methods allow the traditional taxonomic confirmation of this pathovar within the species. Serological and molecular techniques such as: ELISA tests, immunofluorescence, polymerase chain reaction (PCR) are also used for the characterization and detection of the bacterium. Such techniques are very useful and sensible for the diagnostic. |
| Tipo: Journal article |
Palavras-chave: Pseudomonas syringae pv. tabaci; Rep-PCR; Pathogenicity; Serology. |
| Ano: 2007 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000300001 |
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| Percedo,María Irian; Alfonso,P; Frías,María Teresa; Díaz de Arce,Heydi; Barrera,Maritza; Fonseca,O; Castell,Sara. |
| Vaccination against classical swine fever (CSF) is an essential measure for its control in endemic areas, and the Chinese Strain has been used with success in already disease free countries. Nevertheless the complexity of the factors interacting on the epidemiology and control of CSF, when the expected success is not reached with the vaccination of premises, doubts always fall on the vaccine used and the vaccination scheme applied In consecuences, many changes in vaccination scheme by the breeders are frecuently observed whitout positive influence in CSF control. To clarify the CSF recurrence in a herd vaccinated by a different scheme as the one recommended in the national control program, serological samples were taken to different animal groups in three... |
| Tipo: Journal article |
Palavras-chave: Classical swine fever; Antibodies; Vaccination; Swine; Serology; Focus. |
| Ano: 2009 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300004 |
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| Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.. |
| In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012 |
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| PAZ,Giselle Souza da; ROCHA,Katarine de Souza; LIMA,Michele de Souza; JORGE,Ediene Moura; PANTOJA,José Carlos Figueiredo; MORAES,Carla Cristina Guimarães de; LANGONI,Helio. |
| Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira spp.; Brucella spp.; Domestic canids; Serology. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672015000300265 |
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| Paredes,J.C.M.; Oliveira,E.A.S.; Oliveira,L.G.; Rosa,J.C.A.; Roehe,P.M.. |
| Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Classical swine fever; Pestivirus; Serology. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500001 |
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| Megid,J.; Brito,A.F.; Moraes,C.C.G.; Fava,N.; Agottani,J.. |
| Observou-se brucelose canina em quatro canis diferentes. Os canis apresentaram animais com história de aborto, mortalidade em neonatos e nascimentos prematuros. A porcentagem de animais soropositivos para brucelose canina, pela prova de imunodifusão em ágar gel, variou de 4,6 a 57,1%. Observou-se correlação positiva entre porcentagem de animais positivos e aspectos reprodutivos e condições de aglomeração. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dog; Brucellosis; Epidemiology; Serology. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500007 |
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| Bulos,L.H.S.; Gonzaga,N.F.; Souza,L.F.L.; Assao,V.S.; Leite,R.A.S.; Rebouças,M.S.; Scatamburlo,T.M.; Guimarães,J.D. Guimarães S.E.F.; Lopes,P.S.; Almeida,M.R.L.; Silva Júnior,A.. |
| ABSTRACT Brazilian pig population is made up of several naturalized breeds; among them the Piau breed is known for its rusticity and large fat stores. The naturalized breeds, in comparison with commercial ones, may have an increased resistance to diseases circulating in their territory. Thus, this study aimed to verify if there are differences between the serologic profile against Porcine circovirus 2 (PCV2) of Piau pigs and that of a commercial breed from a farm naturally infected by PCV2. The serum viral load was measured by qPCR, and levels of anti-PCV2 antibodies were measured by ELISA. The results showed that the serum viral load was similar across all animals. However, Piau piglets showed higher levels of antibodies compared to commercial piglets (P=... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serum viral load; Serology; Piau. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501145 |
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| Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L.. |
| A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031 |
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| Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.. |
| ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077 |
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| Ceran,Nurgul; Turkoglu,Recai; Erdem,Ilknur; Inan,Asuman; Engin,Derya; Tireli,Hulya; Goktas,Pasa. |
| Brucellosis is a zoonotic infection and has endemic characteristics. Neurobrucellosis is an uncommon complication of this infection. The aim of this study was to present unusual clinical manifestations and to discuss the management and outcome of a series of 18 neurobrucellosis cases. Initial clinical manifestations consist of pseudotumor cerebri in one case, white matter lesions and demyelinating syndrome in three cases, intracranial granuloma in one case, transverse myelitis in two cases, sagittal sinus thrombosis in one case, spinal arachnoiditis in one case, intracranial vasculitis in one case, in addition to meningitis in all cases. Eleven patients were male and seven were female. The most prevalent symptoms were headache (83%) and fever (44%). All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neurobrucellosis; Serology; Diagnosis; Neuroimaging. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100010 |
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| Registros recuperados: 57 | |
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