|
|
|
| Registros recuperados: 61 | |
|
|
| I examined the multiple visions of the future of the city that can emerge when city actors and organizations reconfigure themselves to address sustainability. In various cities worldwide, novel ideas, initiatives, and networks are emerging in governance to address social and ecological conditions in urban areas. However, cities can be contested spaces, bringing a plurality of actors, network configurations, preferences, and knowledge that shape the politics over desirable pathways for future development. I used the knowledge-action systems analysis (KASA) approach to examine the frames and knowledge systems influencing how different actors involved in the land governance network of the city of San Juan constructed visions for the future of the city.... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Adaptive capacity; Adaptive governance; Frames; Knowledge-action systems; Networks; San Juan; Sustainable pathways; Transformation; Urban social-ecological systems; Visions. |
| Ano: 2014 |
|
| |
|
| |
|
|
| The persistence of current societal problems has given rise to a quest for transformative social innovations. As social innovation actors seek to become change makers, it has been suggested that they need to play into impactful macrodevelopments or “game-changers”. Here, we aim to deepen the understanding of the social innovation agency in these transformation games. We analyze assumptions about the game metaphor, invoking insights from actor-network theory. The very emergence of transformation games is identified as a crucial but easily overlooked issue. As explored through the recent electricity blackout threat in Belgium, some current transformation games are populated with largely passive players. This illustrative case demonstrates... |
| Tipo: Peer-Reviewed Synthesis |
Palavras-chave: Actor network theory; Electricity grid; Enrolment; Game-changers; Social innovation; Transformation. |
| Ano: 2016 |
|
| |
|
|
| Abel, Nick; Land and Water, Commonwealth Scientific and Industrial Research Organisation ; nick.abel@csiro.au; Wise, Russell M.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; russell.wise@csiro.au; Colloff, Matthew J.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Matt.Colloff@csiro.au; Walker, Brian H.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; brian.walker@csiro.au; Butler, James R. A.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; james.butler@csiro.au; Ryan, Paul; Australian Resilience Centre; paulryan@internode.on.net; Norman, Chris; Goulburn Broken Catchment Management Authority; chrisn@gbcma.vic.gov.au; Langston, Art; Land and Water, Commonwealth Scientific and Industrial Research Organisation; art.langston@csiro.au; Anderies, John M.; Arizona State University; m.anderies@asu.edu; Gorddard, Russell; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Russell.Gorddard@csiro.au; Dunlop, Michael; Land and Water, Commonwealth Scientific and Industrial Research Organisation; michael.dunlop@csiro.au; O'Connell, Deborah; Land and Water, Commonwealth Scientific and Industrial Research Organisation; deborah.o'connell@csiro.au. |
| Climate change and its interactions with complex socioeconomic dynamics dictate the need for decision makers to move from incremental adaptation toward transformation as societies try to cope with unprecedented and uncertain change. Developing pathways toward transformation is especially difficult in regions with multiple contested resource uses and rights, with diverse decision makers and rules, and where high uncertainty is generated by differences in stakeholders’ values, understanding of climate change, and ways of adapting. Such a region is the Murray-Darling Basin, Australia, from which we provide insights for developing a process to address these constraints. We present criteria for sequencing actions along adaptation pathways: feasibility... |
| Tipo: Peer-Reviewed Insight |
Palavras-chave: Adaptation pathways; Climate change; Collective action; Domain shift; Equity; Irrigation; Resilience; Social conflict; Transformation; Wetlands. |
| Ano: 2016 |
|
| |
|
|
| Yongzhong,Lu; Zhang,Xuecheng. |
| In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the... |
| Tipo: Journal article |
Palavras-chave: CpcB gene; Deletion analysis; Light intensity; Synechococcus; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100010 |
| |
|
|
| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
| |
|
|
| Tsay,Hsin-Sheng; Ho,Hui-Min; Gupta,Sushim K; Wang,Chang-Sheng; Chen,Pei-Tsu; Chen,Emily Chin-Fun. |
| In the present study, a novel plant transformation system for Doritaenopsis and Phalaenopsis has been developed. The pollen-mediated activation tagging system was established by artificial pollination. The pollens, co-cultured with Agrobacterium tumefaciens strain EHA105 harbouring an activation tagging vector (pTAG-8), were used for pollination. In order to optimize the transformation efficiency, several factors (concentration of A. tumefaciens, concentration of acetosyringone during co-cultivation and the duration of co-cultivation) known to influence Agrobacterium-mediated DNA transfer were examined. A concentration of 0.5-1 x 10(8) CFU/ml for A. tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were found to be the optimal condition... |
| Tipo: Journal article |
Palavras-chave: Activation tagging; Agrobacterium tumefaciens; Doritaenopsis cultivars; Phalaenopsis amabilis; Transformation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400009 |
| |
|
| |
|
| |
|
|
| Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Kumar,Vinod; Lakshmanan,Venkatachalam; Venkataramareddy,Sreedhar Reddampalli; Aswathanarayana,Ravishankar Gokare. |
| The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium rhizogenes; Auxins; Elicitation; T-DNA; Thermostability; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008 |
| |
|
|
| Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.. |
| The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022 |
| |
|
| |
|
|
| Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael. |
| Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation:... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395 |
| |
|
|
| ANDRADE, S. R. M.; GLORIA, B. A.; LABATE, M. T. V.; GOMES, D. G.; LABATE, C. A.. |
| Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Transformation; SAAT; Common bean; Biotecnologia: transgenico.; Bactéria; Cerrado; Feijão; Infecção; Genética Vegetal; Phaseolus Vulgaris.; Plant genetics.. |
| Ano: 2001 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567067 |
| |
|
|
| Bruiant, Laure. |
| The aim of this study of the shad Vi/osa sp.) is to determine the flesh's characteristics in relation with fishing are as and catching periods as weIl as the best storage conditions of the fresh and frozen fish, in order to predict the resulting changes with the eventual aim of increasing the market consumption. Chemical, bacteriological and sensorial analysis have been assessed in order to follow the change in the product during the storage. The frozen fish will be analysed until July 1992. Some tests to rem ove bones, using a machine designed for carp fillets, have been done with the aim of adjusting the process to the shad. At the end of the study we have given advice to the fishermen concerning the conditions necessary to keep the fish fresh and we... |
| Tipo: Text |
Palavras-chave: Alose (alosa sp.); Valorisation; Transformation; Désarêtage. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00120/23099/20948.pdf |
| |
|
| |
|
|
| Penven, Anais. |
| Beyond the technical and technological advances in the conversion of marine by-products into useful products, the territorial, economic, social, political and environmental parameters, which govern all forms of approaches for fish by-products upgrading, must be taken in account. This is essential to understand why and how bioconversion technologies are applicable. The fishery sector is facing several issues such as fish stocks weakening, recourse to imports, aquaculture rising or consumers behaviors which complicate the approach of fish by-products upgrading as it is linked to the raw material processing. Thanks to some projects addressed this thematic in recent years, several quantitative and qualitative data about by-products deposits are available.... |
| Tipo: Text |
Palavras-chave: Sous-produits; Valorisation; Territoire; Pêche; Gestion; Transformation; Stratégie industrielle; Multi-filières; By-products; Upgrading; Territory; Fishing; Management; Processing; Industrial strategy; Agrowastes. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00212/32347/30796.pdf |
| |
|
|
| Eymard, Sylvie. |
| Critical steps of the process of horse mackerel surimi manufacturing were identified. This led to propose technical and chemical solutions in order to limit development of lipid oxidation reactions. In this way, analytical methods of lipids and lipid oxidation compounds were adapted to the studied products. A method to determine hydroperoxyde concentrations was developed. The front face fluorescence technique was tested to evaluate the degree of surimi alteration. Lipid oxidation kinetics were determined during horse mackerel storage on ice or at 17°C. The formation of lipid oxidation products was followed during manufacturing and horse mackerel surimi frozen storage. The impact of raw material freshness on the development of lipid oxidation during surimi... |
| Tipo: Text |
Palavras-chave: Processing; Conservation; Surimi; Pelagic fish; Lipid oxidation; Lipids; Transformation; Conservation; Surimi; Petits pélagiques; Oxydation des lipides; Lipides. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/these-2.pdf |
| |
|
| |
| Registros recuperados: 61 | |
|
|
|
