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| Registros recuperados: 50 | |
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| Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle. |
| Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus.... |
| Tipo: Text |
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf |
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| Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra. |
| In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF |
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| Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine. |
| In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was... |
| Tipo: Text |
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf |
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| Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine. |
| Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French... |
| Tipo: Text |
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf |
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| Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R. |
| 110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf |
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| De Decker, Sophie. |
| The bacteria of the Vibrio genus are one of the most abundant groups in the aquatic ecosystems. Two species of vibrios, Vibrio splendidus and V. Aestuarianus, have been associated, in some cases, to mortality in flat oyster Crassostrea gigas farms, most of the time during the summer period. This doctoral thesis studies the Vibrio-host interaction and their modulations according to the host's pathogenic virulence and its genetic, physiologic and immunitary parameters. These factors will be characterised by in vitro and in vivo approaches (experimental infection). The characterization of contrasted genetic families according to their sensitivity to these vibrios will enable the implementation of new improvement strategies in oyster farms (prophylactic... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres génétiques; Virulence; Oyster; Crassostrea gigas; Pathogène; Vibrio. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3431.pdf |
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| Falaise, Charlotte; James, Adele; Travers, Marie-agnes; Zanella, Marie; Badawi, Myriam; Mouget, Jean-luc. |
| Marennine, the water-soluble blue pigment produced by the marine diatom Haslea ostrearia, is known to display antibacterial activities. Previous studies have demonstrated a prophylactic effect of marennine on bivalve larvae challenged with a pathogenic Vibrio splendidus, suggesting that the blue Haslea is a good candidate for applications in aquaculture as a source of a natural antimicrobial agent. Indeed, the genus Vibrio is ubiquitous in aquaculture ecosystems, and regular events of pathogenic invasion cause some of the biggest losses worldwide. To better characterize the effects of marennine on Vibrios, a panel of 30 Vibrio strains belonging to 10 different species was tested, including bivalve pathogenic species (e.g., Vibrio crassostreae and Vibrio... |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Diauxie; Haslea; Hormesis; Marennine; Vibrio. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00484/59598/62602.pdf |
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| De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis. |
| Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and... |
| Tipo: Text |
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf |
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| Mersni Achour, Rachida; Imbert-auvray, Nathalie; Huet, Valerie; Ben Cheick, Yosra; Faury, Nicole; Doghri, Ibtissem; Rouatbi, Sonia; Bordenave, Stéphanie; Travers, Marie-agnes; Saulnier, Denis; Fruitier-arnaudin, Ingrid. |
| Extracellular products (ECPs) of the French V. tubiashii strain 07/118 T2 were previously reported to be toxic for the Pacific oyster Crassostrea gigas. In this study we now assessed host cellular immune responses and bacterial potential effectors by which these ECPs can be associated with host damages. The adhesion capacity (28% inhibition) and phagocytosis ability (56% inhibition) of oyster hemocytes were the main functions affected following in vitro contact between hemocytes and V. tubiashii ECPs. This may be linked to the demonstration of the capability of ECPs to cleave various cellular substrates as oyster collagen. Moreover, a strong metalloproteolytic activity was recorded with general (azocasein) and specific (ADAM) substrates and characterized... |
| Tipo: Text |
Palavras-chave: ECPs; Oyster immune responses; Thermolysin-metalloprotease; Mass spectroscopy; ADAM substrate; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00211/32203/30642.pdf |
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| Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel. |
| Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La.... |
| Tipo: Text |
Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf |
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| Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet. |
| Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence... |
| Tipo: Text |
Palavras-chave: Probiotic; Pediococcus acidilactici; Shrimp; Oxidative stress; Antioxidant status; Vibrio; Immersion challenge. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11419/7984.pdf |
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| Rolland, Jean-luc; Abdelouahab, Mahdia; Dupont, J.; Lefevre, F.; Bachere, Evelyne; Romestand, Bernard. |
| The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M < MIC <2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M < MIC <80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent... |
| Tipo: Text |
Palavras-chave: Ls-Stylicin 1; Peptide; Shrimp; Antifungal; Agglutination; Vibrio. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11387/8094.pdf |
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| Goudenege, David; Labreuche, Yannick; Krin, Evelyne; Ansquer, Dominique; Mangenot, Sophie; Calteau, Alexandra; Medigue, Claudine; Mazel, Didier; Polz, Martin F.; Le Roux, Frederique. |
| Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively... |
| Tipo: Text |
Palavras-chave: Horizontal gene transfer; Nigritoxin; Phylogeny; Shrimp; Virulence; Vibrio. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27117/28369.pdf |
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| Le Groumellec, M.; Haffner, Philippe; Martin, B.; Martin, C.. |
| Mass mortality associated with luminescence has been dbserved in sorne Thai and Ecuadorian penaeid shrimp hatcheries in recent years. Samples collected from hatcheries in these two different areas revealed luminescent sucrose-negative Vibrio strains in larVal rearing tanks where mortality occurred. Pathogenicity trials were carried out to identify the causative agents of mortality and to study the course of infection . . Results showed that strains isolated from Thai hatclleries were more virulent than Ecuadorian strains when tested on different larval stages of Penaeus monodon. The virulence of these strains was also estimated. Trials on other species ofpenaeid shrimp demonstrated the specifie action of Thai strains. Drug sensitivity patterns revealed a... |
| Tipo: Text |
Palavras-chave: Mass mortality; Bacterial; Vibrio; Hatcheries; Penaeus monodon; Shrimp. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/acte-6100.PDF |
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| Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine. |
| In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were... |
| Tipo: Text |
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Registros recuperados: 50 | |
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