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	Provedor de dados BJM (1) Autor Irfan,Muhammad (1) Nadeem,Muhammad (1) Syed,Quratualain (1) Palavra-chave Ethanol production (1) Lignoccellulosic biomass (1) Pretreatment (1) Sacchromyces cervisae (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 404 Primeira ... 13 14 15 16 17 18 19 20 21 ... Última Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais Arq. Bras. Med. Vet. Zootec. Headley,S.A.; Santos,T.R.; Bodnar,L.; Saut,J.P.E.; Silva,A.P.; Alfieri,A.F.; Medeiros,A.A.; Soares,N.P.; Alfieri,A.A.. This study investigated the occurrence of canine distemper virus (CDV) by evaluating the presence of viral RNA within urine samples of dogs from Uberlândia, MG, with clinical manifestations suggestive of infection by CDV by targeting the CDV N gene. Of the clinical samples collected ( n =33), CDV viruria was detected in 45.5%. Five dogs died spontaneously; all had characteristic CDV-associated histopathological alterations and demonstrated CDV viruria. Statistical analyses revealed that the age, gender, breed, or the organ system of the dog affected had no influence on the occurrence of canine distemper. Myoclonus and motor incoordination were the most significant neurological manifestations observed. A direct association was observed between... Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine distemper; Viruria; Phylogeny; Epidemiology. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601510 Phylogeny of canine coronavirus (CCoV) from Brazilian dogs based on membrane protein partial sequences Arq. Bras. Med. Vet. Zootec. Guirao,M.P.; Souza,S.P.; Jerez,J.A.; Richtzenhain,L.J.; Brandão,P.E.. Este artigo descreve a anteriormente desconhecida diversidade molecular de amostras brasileiras de Coronavírus canino (CCoV). Vinte e duas amostras foram submetidas à análise da sequência parcial do gene codificador da proteína de membrana, sendo 12 classificadas como CCoV Tipo II e 10 como CCoV Tipo I e uma possível sublinhagem tipicamente brasileira foi encontrada para o CCoV Tipo II. Tipo: Info:eu-repo/semantics/article Palavras-chave: Coronavirus; Dogs; Phylogeny; Membrane; Brazil. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000600042 Molecular diagnosis of Hepatozoon canis in symptomatic dogs in the city of Goiania, Goiás, Brazil Arq. Bras. Med. Vet. Zootec. Duarte,S.C.; Parente,J.A.; Silveira Neto,O.J.; Jayme,V.S.; Bastos,T.S.A.; Linhares,G.F.C.. ABSTRACT More than 300 species have been described in the genus Hepatozoon, occurring in different vertebrates. Among these, only Hepatozoon canis and Hepatozoon americanum are seen in dogs. Different methods may be used for laboratory diagnosis. The most common of these is direct parasitological examination of parasite stages in blood smears. The aim of this investigation was to conduct a phylogenetic study on Hepatozoon isolates from symptomatic dogs in the city of Goiânia, Goiás, Brazil. Blood samples were obtained from 40 symptomatic dogs that had been referred to the Veterinary Hospital of the Federal University of Goiás. Among these, only two samples were positive for Hepatozoon spp. using the direct parasitological method. These samples were then... Tipo: Info:eu-repo/semantics/article Palavras-chave: Canis familiaris; Hepatozoonosis; Molecular epidemiology; Phylogeny. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601431 Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs Arq. Bras. Med. Vet. Zootec. Santos,A.S.; Lima,D.C.V.; Fernandes,E.F.T.S.; Albuquerque,P.P.F.; Gouveia,G.V.; Sá,M.C.A.; Costa,M.M.; Pinheiro Júnior,J.W.; Mota,R.A.. ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig industry; Colibacillosis; Phylogeny; Antibiogram; Multi-resistance. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309 Molecular analysis of the N gene of canine distemper virus in dogs in Brazil Arq. Bras. Med. Vet. Zootec. Castilho,J.G.; Brandão,P.E.; Carnieli Jr,P.; Oliveira,R.N.; Macedo,C.I.; Peixoto,Z.M.P.; Carrieri,M.L.; Kotait,I.. Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil. Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Canine distemper virus; RT-PCR; Phylogeny. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016 Phylogenetic analysis of Pneumocystis from pig lungs obtained from slaughterhouses in southern and midwestern regions of Brazil Arq. Bras. Med. Vet. Zootec. Sanches,E.M.C; Ferreiro,L; Borba,M.R; Spanamberg,A; Ravazzolo,A.P; Santurio,J.M; Driemeir,D; Barcellos,D.E.S.N; Berthelemy,M; Guillot,J. The Pneumocystis genus is comprised of pathogens dwelling in the lungs of terrestrial, aerial, and aquatic mammals. Occasionally they induce severe pneumonitis, particularly in hosts with severe impairment of the immune system and progressively may fill pulmonary alveolar cavities causing respiratory failure. Molecular genetic studies revealed that Pneumocystis gene sequences present a marked divergence with the host species concerned. In the present study, the genetic diversity of Pneumocystis obtained from lungs of swines was examined by analyzing mitochondrial large subunit (mtLSU) and small subunit (mtSSU) rRNA sequences. The samples were obtained from two slaughterhouses located in two Brazilian states. Phylogenetic analysis demonstrated that genetic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Pneumocystis; Phylogeny; Mitochondrial large subunit; Small subunit; RRNA. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500016 Phylogenetic analysis of feline immunodeficiency virus strains from State of Minas Gerais, Brazil Arq. Bras. Med. Vet. Zootec. Caxito,F.A.; Coelho,F.M.; Oliveira,M.E.; Resende,M.. A região p17-p24 do gene gag de 10 amostras do vírus da imunodeficiência felina detectadas no estado de Minas Gerais (Brasil) foi seqüenciada com o objetivo de determinar a sua classificação molecular e a sua relação com seqüências de amostras previamente descritas. As amostras pertenciam ao subtipo B, entretanto foi possível observar que a maioria delas encontra-se em um subgrupo dentro do subtipo B, o que indica presença de um possível ancestral comum entre elas. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cat; Feline immunodeficiency; Phylogeny; Gag gene. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600035 A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata Biocell Vega,Israel A.; Dellagnola,Federico A.; Hurst,Jorge A.; Godoy,Martín S.; Castro-Vazquez,Alfredo. Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Symbiosis; Cyanobacteria; Chloroplast; Photosynthetic pigments; Phylogeny. Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200001 Karyotype description of Pomacea patula catemacensis (Caenogastropoda, Ampullariidae), with an assessment of the taxonomic status of Pomacea patula Biocell Diupotex-Chong,María Esther; Cazzaniga,Néstor J.; Hernández-Santoyo,Alejandra; Betancourt-Rule,José Miguel. Mitotic chromosomes of the freshwater snail Pomacea patula catemacensis (Baker 1922) were analyzed on gill tissue of specimens from the type locality (Lake Catemaco, Mexico). The diploid number of chromosomes is 2n = 26, including nine metacentric and four submetacentric pairs; therefore, the fundamental number is FN = 52. No sex chromosomes could be identified. The same chromosome number and morphology were already reported for P. flagellata, i.e., the other species of the genus living in Mexico. The basic haploid number for family Ampullariidae was reported to be n = 14 in the literature; so, its reduction to n = 13 is probably an apomorphy of the Mexican Pomacea snails. Lanistes bolteni, from Egypt, also shows n = 13, but its karyotype is much more... Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple snails; Chromosomes; Taxonomy; Phylogeny; Mexico. Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300004 Comparative fine structure of the epididymal spermatozoa from three korean shrews with considerations on their phylogenetic relationships Biocell Soon-Jeong,Jeong; Joo-Cheol,Park; Heung-Joong,Kim; Chun,Sik Bae; Myung-Hee,Yoon; Do-Seon,Lim; Moon-Jin,Jeong. This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew ( Crocidura suaveolens ), the Japanese white-toothed shrew ( C. dsinezumi ) and the big white-toothed shrew ( C. lasiura ) belonging to the subfamily Crocidurinae living in Korea . In the spermatozoa of C. suaveolens , the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura , the head has a large acrosome, a serrated inner acrosomal membrane and a common apical body.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Crocidurinae; Shrew; Spermatozoa; Phylogeny; Fine structure. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200003 Systematic revision of the South American silversides (Teleostei, Atheriniformes) Biocell Brian,S.; Dyer,H.. Tipo: Info:eu-repo/semantics/article Palavras-chave: Atherinopsinae; Odontesthes; Phylogeny; Taxonomy; Morphology. Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100010 The rise and fall of the Refugial Hypothesis of Amazonian speciation: a paleoecological perspective Biota Neotropica Bush,Mark B.; Oliveira,Paulo E. de. The refugial hypothesis is treated as the definitive history of Amazonian forests in many texts. Surprisingly, this important theoretical framework has not been based on paleoecological data. Consequently, a model of Amazonian aridity during the northern hemispheric glaciation has been accepted uncritically. Ironically, the Refuge Hypothesis has not been tested by paleobotanical data. We present a revision of the concept of Neotropical Pleistocene Forest Refuges and test it in the light of paleocological studies derived from pollen analysis of Amazonian lake sediments deposited during the last 20,000 years. Our analysis is based primarily on paleoenvironmental data obtained from sites in Brazil and Ecuador. These data are contrasted with those that favor... Tipo: Info:eu-repo/semantics/other Palavras-chave: Amazonia; Refugia; Fossil pollen; Glaciations; Pleistocene; Holocene; Miocene; Phylogeny; Speciation diversity. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032006000100002 Morphological and molecular characterization of Curvularia lunata pathogenic to andropogon grass Bragantia Santos,Patrícia Resplandes Rocha dos; Leão,Evelynne Urzêdo; Aguiar,Raimundo Wagner de Souza; Melo,Maruzanete Pereira de; Santos,Gil Rodrigues dos. ABSTRACT The fungal genus Curvularia is associated with a number of diseases in plants, commonly producing foliar spots in forage grasses. The objective of this study was to characterize the morphological and molecular diversity of the isolates of Curvularia sp. associated with Andropogon seeds, and to assess both their capacity to transmit disease and the pathogenicity of this fungus to crop. Ten isolates of Curvularia sp. were sourced from Andropogon seeds from agricultural producing regions in the Brazilian states Tocantins and Pará. Morphological characterization was achieved by observing fungus colonies and conidia and molecular characterization by DNA extraction and amplification with sequence-specific primers. The disease transmission was evaluated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Andropogon L.; Curvularia lunata; Diagnosis; Phylogeny; Pathogen. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200326 Genome-Wide Assessment of Putative Superoxide Dismutases in Unicellular and Filamentous Cyanobacteria BABT Prajapati,Rajesh; Yadav,Shivam; Mitra,Sonali; Rai,Priya; Mishra,Rajeev; Atri,Neelam. Abstract Cyanobacteria are photoautotrophic prokaryotes capable to grow in diverse ecological habitats, originated 2.5-3.5 billion years ago and were first to produce oxygen. Since then superoxide dismutases (SOD) acquired great significance due to their ability to catalyze detoxification of byproducts of oxygenic photosynthesis i.e. superoxide radicals. In the present study, we extracted information regarding SODs from species of sequenced cyanobacteria and investigated their diversity, conservation, domain structure, and evolution. 144 putative SOD homologs were identified. Unlike other protein families (ex. serine-threonine kinases) SODs are present in all cyanobacterial species reflecting their significant role in survival. However, their distribution... Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide dismutases; Cyanobacteria; Comparative genomics; Phylogeny. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100422 Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification BABT Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício. The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018 Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG BABT Sado,Ricardo Yuji; Matushima,Eliana Reiko. The aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with BCG in the primitive Arius sp. fish. The BCG was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. As the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the BCG. These giant cells were positive for the S100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Arius; Phylogeny; BCG; Inflammation; Giant cells. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500009 Influenza A and B in a cohort of outpatient children and adolescent with influenza like-illness during two consecutive influenza seasons BJID Machado,Clarisse M.; de Souza,Ana Carolina Mamana Fernandes; Romano,Camila Malta; dos Santos Freire,Wilton; Costa,Ângela Aparecida; Figueiredo,Walter Manso; Pannuti,Cláudio S.; Luna,Expedito J.A.. ABSTRACT Introduction Influenza is an important cause of morbimortality worldwide. Although people at the extremes of age have a greater risk of complications, influenza has been more frequently investigated in the elderly than in children, and inpatients than outpatients. Yearly vaccination with trivalent or quadrivalent vaccines is the main strategy to control influenza. Objectives Determine the clinical and molecular characteristics of influenza A and B infections in children and adolescents with influenza-like illness (ILI). Methods: A cohort of outpatient children and adolescents with ILI was followed for 20 months. Influenza was diagnosed with commercial multiplex PCR platforms. Results: 179 patients had 277 episodes of ILI, being 79 episodes of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Influenza; Outpatient; Children; Vaccine; Lineages; Phylogeny; Cohort. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100073 Hepatitis C virus: molecular and epidemiological evidence of male-to-female transmission BJID Cavalheiro,Norma de Paula; de La Rosa,Abel; Elagin,Slava; Tengan,Fatima Mitiko; Barone,Antonio Alci. INTRODUCTION: There is general consensus that hepatitis C virus is efficiently transmitted by the parenteral route, whereas data on viral transmission by sexual or non-sexual intrafamilial contact are conflicting. OBJECTIVE AND METHOD: The aim of this study was to investigate the transmission of hepatitis C virus in nine heterosexual couples. RESULT: The mean age of the couples was 43.7 years. When interviewed, all of the women denied the presence of risk factors for acquisition of the infection, whereas the cause of infection in the nine husbands could be attributed to blood transfusions in two of them (22.2%), use of intravenous and inhaled drugs in six (66.7%), acupuncture in one (11.1%), and tattooing in one (11.1%). All men and none of the women... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sexually transmitted diseases; Phylogeny; Hepatitis C. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500001 Desmodus rotundus and Artibeus spp. bats might present distinct rabies virus lineages BJID Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo. In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008 Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic) BJM Rulík,Martin; Chaudhary,Prem Prashant. Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Magnetotactic bacteria; Sediment; PCR; Cloning; Phylogeny; 16S rRNA gene. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016 Registros recuperados: 404 Primeira ... 13 14 15 16 17 18 19 20 21 ... Última

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