|
|
|
| Registros recuperados: 52 | |
|
|
| Le Goic, Nelly; Hegaret, Helene; Boulais, Myrina; Beguel, Jean-philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe. |
| The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Gamete quality; ROS production; Viability; Flow cytometry; Ecotoxicology. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00216/32760/31321.pdf |
| |
|
| |
|
| |
|
|
| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre. |
| Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence... |
| Tipo: Text |
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy. |
| Ano: 2006 |
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf |
| |
|
|
| Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goic, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-pont, Ika; Soudant, Philippe; Huvet, Arnaud. |
| Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS... |
| Tipo: Text |
Palavras-chave: Microplastic; Reproduction; Energy allocation; Oyster. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00311/42233/41600.pdf |
| |
|
|
| Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika. |
| Microplastics (<5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges... |
| Tipo: Text |
Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf |
| |
|
|
| Richard, Gaelle; Guerard, Fabienne; Corporeau, Charlotte; Lambert, Christophe; Paillard, Christine; Pernet, Fabrice. |
| We investigated the effect of brown ring disease (BRD)1 development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio tapetis. At two days post-injection (dpi), activities of superoxide dismutase and glutathione peroxidase (GPx) decreased whereas activities of nitric oxide synthase (iNOS) and catalase increased in infected clams, although no clinical signs were visible (BRD-). At 7 dpi, activities of several antioxidant and immune-related enzymes were markedly increased in BRD-likely indicating an efficient reactive oxygen species (ROS) scavenging compared to... |
| Tipo: Text |
Palavras-chave: Antioxidant enzymes; Immunity; Energetic metabolism; Ruditapes philippinarum; Vibrio tapetis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00316/42684/42057.pdf |
| |
|
|
| Long, Marc; Tallec, Kevin; Soudant, Philippe; Le Grand, Fabienne; Donval, Anne; Lambert, Christophe; Sarthou, Geraldine; Jolley, Dianne F.; Hegaret, Helene. |
| Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition,... |
| Tipo: Text |
Palavras-chave: Allelopathy; Alexandrium; Metabolism; Photoinhibition; Lipids; Pigments. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00460/57133/59748.pdf |
| |
|
|
| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. |
| The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf |
| |
|
|
| Roussel, Sabine; Bish, Thomas; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Lambert, Christophe; Lachambre, Sébastien. |
| The domestication of Haliotis tuberculata began recently. During this domestication process, abalone may acquire behavioural and physiological traits to become more adapted to their captive environment. These modifications could be the result of intentional selection on production traits, or of unintentional selection due to specific conditions experienced in the farm environment. In order to study this process, progenies of 3 different broodstock origins were studied. Wild abalone, farmed abalone selected for faster growth at 5% selection pressure and randomly selected farmed abalone were used as broodstock. Farmed abalone were the third generation of abalone resulting from systematic mating between wild and farmed broodstock (either males or females were... |
| Tipo: Text |
|
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51546/52138.pdf |
| |
|
|
| González-fernández, Carmen; Toullec, Jordan; Lambert, Christophe; Le Goïc, Nelly; Seoane, Marta; Moriceau, Brivaela; Huvet, Arnaud; Berchel, Mathieu; Vincent, Dorothée; Courcot, Lucie; Soudant, Philippe; Paul-pont, Ika. |
| The potential presence of nanoplastics (NP) in aquatic environments represents a growing concern regarding their possible effects on aquatic organisms. The objective of this study was to assess the impact of polystyrene (PS) amino-modified particles (50 nm PSNH2) on the cellular and metabolic responses of the diatom Chaetoceros neogracile cultures at two essential phases of the growth cycle, i.e. exponential (division) and stationary (storage) phases. Both cultures were exposed for 4 days to low (0.05 μg mL−1) and high (5 μg mL−1) concentrations of PS-NH2. Exposure to NP impaired more drastically the major cellular and physiological parameters during exponential phase than during the stationary phase. Only an increase in ROS production was observed at... |
| Tipo: Text |
Palavras-chave: Nanoplastics; Diatoms; Physiology; Transparent exopolymer particles. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60235/63627.pdf |
| |
|
|
| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Giudicelli, Emilie; Lambert, Christophe; Seguineau, Catherine; Samain, Jean-francois. |
| Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by... |
| Tipo: Text |
Palavras-chave: Hemocyte parameters; Oyster immunity; Arachidonic Acid; Poly unsaturated fatty acids; Nutrition; Bivalve; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1876.pdf |
| |
| Registros recuperados: 52 | |
|
|
|
