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Agronomic and molecular characterization of wild germplasm Saccharum spontaneum for sugarcane and energycane breeding purposes Scientia Agricola
Silva,Jorge Alberto Gonçalves da; Costa,Paulo Mafra de Almeida; Marconi,Thiago Gibbin; Barreto,Everton Jean da Silva; Solís-Gracia,Nora; Park,Jon-Won; Glynn,Neil Charles.
ABSTRACT: Among the species related to sugarcane, Saccharum spontaneum (L.) is a wild species with the greatest potential as a source of genetic variation to cope with biomass production in harsh environments. Due to its high yield, early vigor, ratooning ability, low input requirements and tolerance to various biotic and abiotic stresses, sugarcane breeders have shown interest in its contribution, as a donor of genes, to the development of high biomass energy canes. The conservation, evaluation and utilization of the genetic variability of S. spontaneum available in germplasm collections are critical for breeding, but, given the aggressive rhizomatous growth habit and the ability to propagate via seed dispersal, S. spontaneum is classified as a noxious...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Yield; Bioenergy; Abiotic stress; Core collection.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000400329
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Accuracy and simultaneous selection gains for N-stress tolerance and N-use efficiency in maize tropical lines Scientia Agricola
Mendonça,Leandro de Freitas; Granato,Ítalo Stefanine Correia; Alves,Filipe Couto; Morais,Pedro Patric Pinho; Vidotti,Miriam Suzane; Fritsche-Neto,Roberto.
ABSTRACT Maize plants can be N-use efficient or N-stress tolerant. The first have high yields in favorable environments but is drastically affected under stress conditions; whereas the second show satisfactory yields in stressful environments but only moderate ones under optimal conditions. In this context, our aim was to assess the possibility of selecting tropical maize lines that are simultaneously N-stress tolerant and N-use efficient and check for differences between simultaneous selection statistical methods. Sixty-four tropical maize lines were evaluated for Nitrogen Agronomic Efficiency (NAE) and Low Nitrogen Tolerance (LNTI) response indices and two per se selection indices, Low Nitrogen Agronomic Efficiency (LNAE) and Harmonic Mean of Relative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Correlation between traits; Winter maize; Mixed models.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000600481
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ESTs analysis in maize developing kernels exposed to single and combined water and heat stresses Scientia Agricola
Andjelković,Violeta; Ignjatović-Micić,Dragana.
Molecular and metabolic response of plants to a combination of two abiotic stresses is unique and cannot be directly extrapolated from the response of plants to each of the stresses individually. cDNA macroarray has become a useful tool to analyze expression profiles and compare the similarities and differences of various expression patterns. A macroarray of approximately 2,500 maize (Zea mays L.) cDNAs was used for transcriptome profiling in response to single and simultaneous application of water and high temperature stress of maize developing kernels at 15 days after pollination. All stress treatments (water stress-WS, heat stress-HS and their combined application-CS) induced changes in expression of 106 transcripts with 54 up-regulated and 52...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Down-regulation; Macroarray; Up-regulation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300013
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Jatropha curcasand Ricinus communisdisplay contrasting photosynthetic mechanisms in response to environmental conditions Scientia Agricola
Lima Neto,Milton Costa; Martins,Marcio de Oliveira; Ferreira-Silva,Sérgio Luiz; Silveira,Joaquim Albenísio Gomes.
Higher plants display different adaptive strategies in photosynthesis to cope with abiotic stress. In this study, photosynthetic mechanisms and water relationships displayed byJatropha curcasL. (physic nuts) andRicinus communisL. (castor bean), in response to variations in environmental conditions, were assessed.R. communis showed higher CO2 assimilation, stomatal and mesophyll conductance thanJ. curcas as light intensity and intercellular CO2 pressure increased. On the other hand,R. communis was less effective in stomatal control in response to adverse environmental factors such as high temperature, water deficit and vapor pressure deficit, indicating lower water use efficiency. Conversely,J. curcas exhibited higher photosynthetic efficiency (gas exchange...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Castor bean; Photosynthesis; Physic nut; Acclimation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300260
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Gas exchange in 'BRS Princesa' banana ( Musa spp.) under partial rootzone drying irrigation in the north of Minas Gerais, Brazil Acta Agron. (Palmira)
Santos,Marcelo Rocha dos; Rodrigues Donato,Sérgio Luiz; Arantes,Alessandro de Magalhães; Coelho,Eugênio Ferreira; Oliveira,Polyanna Mara de.
Abstract This research aimed to evaluate physiological characteristics of 'BRS Princesa' banana under partial rootzone drying irrigation, PRD. The five treatments, 1 - 50% of reduction in water depth (WD) with a frequency alternation of irrigation side (FA) of seven days during the whole production cycle; 2 - 50% of WD and FA of 14 days in the cycle; 3 - 50% of WD and FA of 21 days; 4 - 50% of WD with a fixed irrigation (only one lateral line on one side of the plant); and 5 - full irrigation (two lateral lines with open shutoff valve) throughout the cycle, they were arranged in a randomized block design with five replicates. There is a reduction in photosynthetic rates and water use efficiency as a result of enzymatic impairment or stomatal closure, due...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Irrigation techniques; Photosynthetic rate; Stomatal closure; Vegetative growth; Water deficit.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000300378
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Effects of vitamin pre-sowing treatment on sweet maize seedlings irrigated with saline water Acta Agron. (Palmira)
Pradi-Vendruscolo,Eduardo; Seleguini,Alexander.
Abstract Salinity stress represents an obstacle for the production of plants of commercial interest, including sweet maize. Among the techniques used to suppress the effects caused by excess salts in the soil is the exogenous application of vitamins. Thus, the objective of this study was to evaluate the effect of the pre-sowing treatment of sweet maize seeds in solutions of thiamine, niacin and these two vitamins combined on the development of the plants irrigated with saline water. The treatments were composed by the 24 h pre-sowing treatment with water, thiamine solution (thiamine 100 mg L-1), niacin solution (niacin 100 mg L-1) and a combined solution with these two vitamins (thiamine 50 mg L-1 + niacin 50 mg L-1) irrigated with saline water. A control...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Defense mechanism; Vitamin B1; Vitamin B3; Zea mays L. saccharata Strut.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122020000100020
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Pollen viability of Tepary bean (Phaseolus acutifolius A. Gray.) mutant lines under water stress conditions and inoculation with rhizobia Acta Agron. (Palmira)
Crespo-Muñoz,Sergio; Rivera-Peña,Mariela; Rosero-Alpala,Deisy Alexandra; Muñoz-Florez,Jaime Eduardo; Rao,Idupulapati M; Muñoz-Florez,Ligia Carmenza.
Abstract Tepary bean, Phaseolus acutifolius A. Gray, is tolerant to drought and high temperatures but has low genetic diversity in its cultivated forms. To increase this diversity, a research program on mutation induction was initiated. The aim of this study was to establish the effect of different agro-morpho-physiological parameters on pollen viability and quantity in 123 mutant lines M5 of P. acutifolius and parental genotypes, by processing micrographs of pollen stained with acetocarmine. The experiment was conducted under greenhouse conditions and the experimental design was a split-split plot with three replicates; in addition, the factors evaluated were as follows: soil moisture conditions, inoculation with Bradyrhizobium sp. or Rhizobium tropici,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; High temperature; Humidity content; Fabaceae; Rhizobiaceae; Inoculation; Mutation induction.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200319
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Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity Acta Botanica
da-Silva,Cristiane J.; Modolo,Luzia V..
ABSTRACT High salinity affects plants due to stimulation of osmotic stress. Cell signaling triggered by nitric oxide (NO) and hydrogen sulfide (H2S) activates a cascade of biochemical events that culminate in plant tolerance to abiotic and biotic stresses. For instance, the NO/H2S-stimulated biochemical events that occur in plants during response to high salinity include the control of reactive oxygen species, activation of antioxidant system, accumulation of osmoprotectants in cytosol, induction of K+ uptake and Na+ cell extrusion or its vacuolar compartmentation among others. This review is a compilation of what we have learned in the last 10 years about NO participation during cell signaling in response to high salinity as well as the role of H2S, a new...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Cell signaling; Hydrogen sulfide; Nitric oxide; NO and H2S biosynthesis; Salt stress.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100150
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Sustainable production of bioactive alkaloids in Psychotria L. of southern Brazil: propagation and elicitation strategies Acta Botanica
Magedans,Yve Verônica da Silva; Rodrigues-Corrêa,Kelly Cristine da Silva; Costa,Cibele Tesser da; Matsuura,Hélio Nitta; Fett-Neto,Arthur Germano.
ABSTRACT Psychotria is the largest genus in Rubiaceae. South American species of the genus are promising sources of natural products, mostly due to bioactive monoterpene indole alkaloids they accumulate. These alkaloids can have analgesic, antimutagenic, and antioxidant activities in different experimental models, among other pharmacological properties of interest. Propagation of genotypes with relevant pharmaceutical interest is important for obtaining natural products in a sustainable and standardized fashion. Besides the clonal propagation of elite individuals, the alkaloid content of Psychotria spp. can also be increased by applying moderate stressors or stress-signaling molecules. This review explores advances in research on methods for plant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Alkaloids; Elicitation; Monoterpenes; Plant propagation; Psychotria; Southern Brazil; Sustainability.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400607
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Genetic responses of traits relationship to components of nitrogen and phosphorus use efficiency in maize - doi: 10.4025/actasciagron.v35i1.15237 Agronomy
DoVale, Júlio César; Universidade Federal de Viçosa; Maia, Ciro; Universidade Federal de Viçosa; Fritsche-Neto, Roberto; Universidade Federal de Viçosa; Miranda, Glauco Vieira; Universidade Federal de Viçosa; Cavatte, Paulo Cézar; Universidade Federal de Viçosa.
This study had three objectives: i) to observe maize inbred lines response to nitrogen (N) and phosphorus (P) acquisition as well as their utilization efficiencies and traits of root morphology in contrasting levels of these nutrients, ii) to study the relationship between root morphology and nutrient use efficiencies for both nutrients, and iii) to identify contrasting parents with components N and P use efficiency for an inheritance study. We evaluated 15 inbred lines in two experiments conducted in contrasting conditions of N and P. We evaluated the shoot, root traits and nutritional efficiencies and observed the genetic variability for most traits. Selection can be practiced for most of these traits in specific environments. Under conditions of...
Tipo: Pesquisa Palavras-chave: Agronomia; Melhoramento Vegetal Zea mays; Acquisition and utilization efficiencies; Root morphology; Abiotic stress; REML/BLUP Genética e melhoramento de plantas Zea mays; Acquisition and utilization efficiencies; Root morphology; Abioticstress; REML/BLUP.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15237
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Expression of genes related to soil flooding tolerance in soybeans Agronomy
Casarotto, Gabriele; Kaspary, Tiago Edu; Cutti, Luan; Thomas, André Luis; Barbosa Neto, Jose Fernandes.
The flooded environment brings about injuries to soybeans that vary depending on the adaptation ability of the genotype. Oxygen deprivation promotes the induction of the expression of genes related to glycolysis and fermentation pathways to maintain energy metabolism and, in addition to reducing-power consuming processes, act in the formation of adaptive structures and the maintenance of the redox status of the plant. The aim of this work was to evaluate the relative expression of genes related to soil flooding response in two contrasting soybean cultivars. Soybean plants of the sensitive (BRS 154) and tolerant (I27) cultivars at the V1 development stage were submitted to the flooding and control conditions (without flooding) for 0, 24, 48, and 96 hours....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max (L.) Merr.; Hypoxia; Abiotic stress; Metabolic alterations; RT-qPCR..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42709
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Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines Agronomy
Marques, Thaís Lima; Pinho, Renzo Garcia Von; Pinho, Édila Vilela de Resende Von; Paniago, Bruno da Costa; Freitas, Natália Chagas; Santos, Heloisa Oliveira dos.
The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Zea mays; QRT-PCR.; Water deficit; Genética Vegetal.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43479
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Evaluation of grain sorghum hybrids for aluminum tolerance in nutrient solution Agronomy
Menezes, Cicero Beserra de; Lima, Geisiane Matos; Marucci, Rosangela Cristina; Bernardino, Karine Costa; Santos, Crislene Vieira dos; Julio, Marcos Paulo Mingote; Schaffert, Robert Eugene.
 Sorghum (Sorghum bicolor L. Moench) is one of the most important cereal crops in the world. In Brazil, the acreage of grain sorghum during off-season is quite expansive. Most of this area is the Cerrado, a Brazilian biome that is similar to a Savannah and is characterized by high acidity and soluble aluminum at toxic levels for plants. The aluminum acts as a limiting factor in achieving high yields. The purpose of this work was to phenotype sorghum hybrids for aluminum tolerance. Eighteen hybrids were evaluated in a nutrient solution containing {0} or {27} µM Al3+. The work was carried out in a growth chamber at the Embrapa Maize and Sorghum, from April 4 to May 30, 2014. The lines ATF 13B (susceptible) and ATF 14B (tolerant) were used as check cultivars....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorghum bicolor; Abiotic stress; Soil acidity; Plant breeding.; Melhoramento Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35304
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The behavior of maize hybrids generated from contrasting progenies regarding the use of nitrogen Agronomy
Guedes, Fernando Lisboa; Ferreira Junior, Edivaldo José; Castro, Carlos Eduardo Caixeta de; Pereira, Carlos Henrique; Prado, Paulo Eduardo Rodrigues; Souza, João Cândido de.
The purpose of this study was to evaluate the performance of maize hybrids synthesized from contrasting genotypes with regard to the use of nitrogen that were selected for their performance in topcrosses. Sixty-seven S0:1 progenies derived from the germplasm bank of Ufla were evaluated in topcross combinations with two testers  at two nitrogen levels. The six progenies with the greatest tolerance and responsiveness to nitrogen (RT) and the five with the least tolerance and responsiveness (RnTn) were selected and, were, afterwards, crossed in a complete diallel, for a total of 55 hybrid combinations. The following genetic parameters were estimated: genetic variance among the hybrids (σ^2G), broad sense heritability in the mean of the hybrids, and selective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.05-9 plant breeding; Abiotic stress; Zea mays Melhoramento Vegetal.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18181
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Evaluation of grain sorghum hybrids for aluminum tolerance in nutrient solution Agronomy
Menezes, Cicero Beserra de; Lima, Geisiane Matos; Marucci, Rosangela Cristina; Bernardino, Karine Costa; Santos, Crislene Vieira dos; Julio, Marcos Paulo Mingote; Schaffert, Robert Eugene.
 Sorghum (Sorghum bicolor L. Moench) is one of the most important cereal crops in the world. In Brazil, the acreage of grain sorghum during off-season is quite expansive. Most of this area is the Cerrado, a Brazilian biome that is similar to a Savannah and is characterized by high acidity and soluble aluminum at toxic levels for plants. The aluminum acts as a limiting factor in achieving high yields. The purpose of this work was to phenotype sorghum hybrids for aluminum tolerance. Eighteen hybrids were evaluated in a nutrient solution containing {0} or {27} µM Al3+. The work was carried out in a growth chamber at the Embrapa Maize and Sorghum, from April 4 to May 30, 2014. The lines ATF 13B (susceptible) and ATF 14B (tolerant) were used as check cultivars....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.05-9 Sorghum bicolor; Abiotic stress; Soil acidity; Plant breeding. Melhoramento Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35304
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High temperatures on root growth and lignification of transgenic glyphosate-resistant soybean Agrociencia
Zonetti,P. da Costa; Suzuki,L. Sayuri; Bonini,E. Aparecida; Ferrarese,M. L. Lucio; Ferrarese-Filho,Osvaldo.
Glyphosate resistant transgenic soybean [Glycine max (L.) Mettill] expresses a glyphosate insensitive EPSPS (5-enolpyruvylshikimate-3-phosphate synthase). This enzyme is involved in important secondary metabolism pathways, including lignin biosynthesis. Thus, differences in lignin content and growth between susceptible (OC14) and glyphosate-resistant soybean may be observed. The objective of this study was to evaluate differences between growth and lignin content of roots in transgenic and OC14 soybean cultivated at high temperatures. Seeds from the OC14 soybean and its transgenic cultivar, CD213RR, were germinated at 25, 27.5, 30 and 32.5 °C. After 3 d, seedlings were cultivated in Hoagland half-strength nutrient solution during 12 h photoperiods and at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glyphosate; Abiotic stress; Glycine max; Plants; Climatic changes.
Ano: 2012 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000600003
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Effects of hormonal priming on seed germination of pigeon pea under cadmium stress Anais da ABC (AABC)
SNEIDERIS,LARISSA C.; GAVASSI,MARINA A.; CAMPOS,MARCELO L.; D'AMICO-DAMIÃO,VICTOR; CARVALHO,ROGÉRIO F..
In this work we investigated whether priming with auxin, cytokinin, gibberellin, abscisic acid and ethylene, alters the physiological responses of seeds of pigeon pea germinated under water and cadmium stress. Seeds treated with water or non-treated seeds were used as control. Although compared to non-treated seeds we found that the hormone treatments improve the germination of pigeon pea under cadmium stress, however, these treatments did not differ from water. However, we also observed a trend of tolerance to the effects of cadmium in the presence of ethylene, suggesting that the use of this hormone may be an efficient method to overcome seed germination under metal stress.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cajanus Cajan; Heavy metal; Hormones; Pretreatment in seeds; Abiotic stress.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401847
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Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots Biocell
Céccoli,Gabriel; Ramos,Julio C.; Ortega,Leandro I.; Acosta,Juan M.; Perreta,Mariel G..
Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations produced by the salt excess. Plants cultivated with the highest level of salinity showed, in the whole, significant differences in the measured...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Tolerance; Vascular tissue.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100002
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Evaluation of common bean genotypes for phosphorus use efficiency in Eutrophic Oxisol Bragantia
Silva,Daiana Alves da; Esteves,Jose Antonio de Fatima; Gonçalves,João Guilherme Ribeiro; Azevedo,Cleber Vinícius Giaretta; Ribeiro,Tamires; Chiorato,Alisson Fernando; Carbonell,Sérgio Augusto Morais.
ABSTRACT Common bean is one of the most important legumes in Latin America, mostly grown in soils with low phosphorus (P) availability. Thus, this study aimed to evaluate the responses of 20 bean genotypes to P deficiency. The experiment was a completely randomized design in a 2 × 20 factorial arrangement; the first factor consisted of P levels and the second factor, of 20 bean genotypes, with six replications. The substrate was a Red Eutrophic Oxisol with low P content. For application of the P treatments, it was applied simple superphosphate, consisting of two levels: restrictive and control, with the application of 45 and 90 kg∙ha–1 of P2O5, respectively. At 28 days, we observed the first symptoms of nutrient deficiency, with the decrease in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Abiotic stress; Plant nutrition; Genotype classification.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000200152
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Rice genotypes for drought tolerance: morphological and transcriptional evaluation of auxin-related genes Bragantia
Madabula,Frederico Pedro; Santos,Railson Schreinert dos; Machado,Nata; Pegoraro,Camila; Kruger,Mariana Madruga; Maia,Luciano Carlos da; Sousa,Rogerio Oliveira de; Oliveira,Antonio Costa de.
ABSTRACT Drought stress affects crop quality and productivity. The challenge of increasing food availability for a growing worldwide demand relies on the development of tolerant cultivars that will need to be adapted to arid and semi-arid areas. In order to help the understanding of rice response to stress, the phenotypic response of 6 Brazilian rice cultivars and 2 different crosses between them were characterized under drought conditions. Since gene regulation is an important part of root morphological responses to stressful conditions, 4 genes related to auxin response and root modifications (OsGNOM1 CRL4, OsIAA1, OsCAND1 and OsRAA1) were evaluated. The expression of these genes was analyzed in stressed rice using public available microarray data and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativ; RT-qPCR; Root; Abiotic stress.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000400428
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