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| Registros recuperados: 53 | |
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| Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova. |
| An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008 |
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| Palacios-Cabrera,Héctor; Taniwaki,Marta Hiromi; Hashimoto,Jorge Minoru; Menezes,Hilary Castle de. |
| The objective of this paper was to determine the influence of three culture media with different water activities, times of incubation and temperatures on the growth of A. ochraceus, A. carbonarius and A. niger. Spores of A. ochraceus, A. carbonarius and A. niger were inoculated onto three culture media: Czapeck Yeast extract Agar (CYA), Dichloran 18% Glycerol Agar (DG18) and Malt Yeast extract 40% Glucose Agar (MY40G). The plates were incubated at five different temperatures (8, 25, 30, 35 and 41ºC). The growth of fungi was evaluated every 24h, measuring the colony diameter (mm). None of the species grew at 8ºC in any culture media. For A. carbonarius, 30ºC was the best temperature for growth while for A. niger temperatures above 30ºC were better in all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus ochraceus; Aspergillus carbonarius; Aspergillus niger; Growth measurement; Water activity; Temperature. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100005 |
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| Milagre,H.M.S.; Martins,L.R.; Takahashi,J.A.. |
| A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae)andenzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stevioside; Hydrolysis; Aspergillus niger; Lipase; Pancreatin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200029 |
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| Gajera,H. P.; Vakharia,D. N. |
| Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days after inoculation (DAI) on PDA media. The specific activities of chitinase, β-1,3-glucanase and protease were 11, 3.46 and 9 folds higher in T6 antagonist (T. viride 60 and A. niger interactions) followed by 8.72, 2.85 and 9 folds in T8 antagonist (T. harzianum 2J and A. niger interactions), respectively, compared to the activity produced by control petri plate T13... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichoderma isolates; Aspergillus niger; Lytic enzymes; Antagonistic interaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100005 |
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| Goulart,Rosangela de Carvalho; Marin,José Moacir. |
| Some filamentous fungi present the phenomenon of dimorphism, their morphological structure alterations being capable of inducing metabolism changes. The Aspergillus niger strain 10v10, a producer of citric acid, was submitted to the mutagenic action of ultraviolet irradiation which respectively selects mutants sensitive or resistant to the antifungi agent 5 fluorocytosine (5-FC). 5-FC sensitive mutants presented a morphological alteration to a yeast-like form. The effects of pH changes, addition of salts (KH2PO4, NH4NO3, MgSO4 and MgCl2), the presence of osmotic stabilizers, as well as of calcium chloride, on morphological reversal and acid production were studied. Morphological reversal to the filamentous form was observed only in the presence of CaCl2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; 5-fluorocytosine; Yeast-like; Calcium; Acid production; Dimorphism. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300018 |
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| Velásquez,Alejandro; Marnet,Pierre-Guy; Arias,Rodrigo. |
| In a first experiment, the effect of in vitro incubation with Aspergillus niger (An) on the chemical composition of different fibrous substrates was studied. In a second experiment, the effect of incubation time (0,72 and 144 h) on in vitro digestion with An of dry matter (IVDMD), neutral detergent fiber (DVNDF) and acid detergent fiber (DVADF) was evaluated for different substrates. Wheat straw, WS; barley straw, BS; oat hulls, OH; dehydrated alfalfa, DA; and dehydrated ryegrass, DB substrates were evaluated. In both experiments, incubations were performed in 250 mL Erlenmeyer flasks; 2 g of substrate was added to a culture medium (pH = 6), and incubated at 28 °C with constant ventilation. The An dose consisted of 3 mL of a solution of 5.3 χ... |
| Tipo: Journal article |
Palavras-chave: Aspergillus niger; Fibrous substrates; In vitro digestion; Nutritional quality. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000100005 |
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| Naves,Luciana de Paula; Corrêa,Angelita Duarte; Bertechini,Antônio Gilberto; Gomide,Elisangela Minati; Abreu,Celeste Maria Patto de. |
| Two distinct experiments were conducted simultaneously with phytases of Aspergillus oryzae and A. niger for determining enzyme activity in response to storage period (up to 180 days at room temperature - RT). In the first experiment, enzymes were stored as they were acquired (pure form) and the activity was measured periodically during 180 days of storage at RT. In the second experiment, the phytases were incorporated to a supplement containing vitamins, minerals, and amino acids; and then this supplement was stored at RT up to 180 days, so that every 30 days of storage was collected one aliquot from each replicate for determining enzymatic activity. In conclusion, the phytase activity is affected by storage duration. To ensure 80% of the initial activity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Aspergillus oryzae; Enzyme; Phytate; Phytic acid. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100029 |
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| Penha,Edmar das Mercês; Viana,Ludmila de Araújo Nunes; Gottschalk,Leda Maria Fortes; Terzi,Selma da Costa; Souza,Erika Fraga de; Freitas,Sidinéa Cordeiro de; Santos,Juliana de Oliveira; Salum,Thaís Fabiana Chan. |
| RESUMO: Neste trabalho, foi avaliado o desempenho de duas linhagens de Aspergillus niger (mutante 11T53A14 e selvagem C) previamente selecionadas como promissoras para a produção de lipases, utilizando meios de cultivo formulados a partir da torta de dendê (palmiste) e da borra alcalina do refino do óleo de dendê (borra de dendê), resíduos provenientes da agroindústria do óleo de dendê (palma) por fermentação em estado sólido (FES). Os experimentos de produção da enzima em FES foram conduzidos em colunas aeradas, incubadas em banho-maria a 32ºC com entrada controlada de ar não umedecido de 1,0vvm. Os melhores resultados foram obtidos com a cepa mutante A. niger 11T53A14 em meio contendo torta de dendê umedecida com solução sulfato de amônio (1,2%) e com a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipase; Resíduo agroindustrial; Aspergillus niger. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400755 |
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| Santos,Tamires Carvalho dos; Cavalcanti,Ingrid Souza; Bonomo,Renata Cristina Ferreira; Santana,Nivio Batista; Franco,Marcelo. |
| The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Pareto charts; Solid state fermentation; Mangifera indica. L.. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200027 |
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| Registros recuperados: 53 | |
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