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| Registros recuperados: 230 | |
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| Renault, Tristan; Arzul, Isabelle. |
| Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf |
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| Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia. |
| Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses... |
| Tipo: Text |
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf |
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| Le Deuff, Rose-marie. |
| Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this... |
| Tipo: Text |
Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf |
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| Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.. |
| Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the... |
| Tipo: Text |
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf |
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| Berthe, Franck; Leroux, Frederique; Adlard, Robert; Figueras, Antonio. |
| Among mollusc pathogens, paramyxeans are an important group of protistan parasites belonging to the genera Marteilia and Marteilioides. Martelia refringens and M. sydneyi are of particular concern given their potential impact on mollusc aquaculture world-wide. Aber disease and QX disease are currently listed by the OIE, the World Organisation for Animal Health. After more than thirty years of existence in the scientific literature, these organisms still pose questions and research challenges to investigators. This paper reviews current knowledge of the group and key references. The review was focused on taxonomy, epidemiology, pathology and potential control methods for these organisms in order to enhance understanding of paramyxean infection issues in... |
| Tipo: Text |
Palavras-chave: Paramyxean parasites; Diagnosis; Pathology; Epidemiology; Taxonomy. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-406.pdf |
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| Renault, Tristan. |
| Global mollusc production is continuously increasing and reached 12.8 million tonnes in 2004 representing 28.2 percent of the total world production valued at US$ 9.4 billions. However, cultivated molluscs may suffer from severe mortality outbreaks. Among the possible causes is the occurrence of infectious diseases due to a variety of pathogens including protozoan parasites, bacteria and viruses. Preventing and controlling diseases has become a priority for aquaculture sustainability. However, molluscs are unique in terms of health management. Molluscs like other invertebrates, lack a true adaptative immune system. Vaccination can not be used to protect them against pathogens. Moreover, molluscs are usually reared in the open sea which strongly limits the... |
| Tipo: Text |
Palavras-chave: Diagnosis; Phylogeny; Genomics; Mollluscs; Pathogens. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4574.pdf |
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| Renault, Tristan; Lipart, Cecile. |
| Several herpes-like virus infections have been reported since 1991 among different bivalve species around the world (Nicolas et al., 1992; Comps and Cochennec, 1993; Renault et al., 1994; Hine and Thome, 1997; Hine 1997). To date, detection of these infections has been strongly correlated with severe losses of oyster larvae and spat. The virus infections are currently diagnosed by laborious histologicai and transmission electron microscopical examinations. These techniques, however, facilitate neither rapid diagnosis nor the ability to analyse large sample numbers. |
| Tipo: Text |
Palavras-chave: Diagnosis; Ostrea edulis; Crassostrea gigas; Herpes like virus. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-2902.pdf |
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| Manterola,Carlos; Cartes-Velasquez,Ricardo; Otzen,Tamara. |
| Globally, the assessment of methodological quality (MQ) in biomedical research is an area of dynamic development over recent years. Understood as a complex and multidimensional construct, various groups have proposed tools for its assessment. Our team has designed and validated scales to assess MQ of therapy and prognosis studies. However, as with other instruments, it is necessary to specify in detail how it is applied, in such a way as to be able to standardize the measurements made with this instrument. A detailed description is presented of the 3 domains (type of research design [single item domain], size of population studied [single item domain], and methodology 7 items) and 9 items comprising the scale, specifying for each item how to assess the... |
| Tipo: Journal article |
Palavras-chave: Investigative Techniques; Methods/epidemiology; Methodological studies; Diagnosis; Diagnostic tests; Reproducibility of Results; Evaluation Studies as Topic; Epidemiological Studies; Evidence-Based Medicine. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100012 |
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| Sassaki,CY; Colodel,MM; Ferreira,I; Nogueira,FS; Lucheis,SB; Langoni,H; Rocha,NS. |
| Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnosis; Dogs; Visceral leishmaniasis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300015 |
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| Camargo,JB; Langoni,H; Troncarelli,MZ; Machado,JG; Lucheis,SB; Padovani,CR. |
| Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Visceral leishmaniasis; Dog; Diagnosis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300007 |
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| Brito,Flávio Gonçalves; Langoni,Helio; Silva,Rodrigo Costa da; Rotondano,Tereza Emmanuelle de Farias; Melo,Marcia Almeida de; Paz,Giselle Souza da. |
| Abstract Background Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors. Methods Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kala-azar; Diagnosis; Dog; Zoonosis; Risk factors. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100311 |
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| Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM. |
| In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003 |
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| Braga,Audrey Rennó Campos; Langoni,Hélio; Lucheis,Simone Baldini. |
| Background: This study aimed to evaluate the occurrence of Leishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Cat; Diagnosis; Leishmaniasis; Zoonosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200323 |
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| Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne Mendonça; Régis-da-Silva,Carlos Gustavo; Brandão Filho,Sinval Pinto; Paiva-Cavalcanti,Milena de. |
| Background : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.Results : Two primer pairs were designed to detect the plasmid pUC18 and a triplex PCR assay targeting theLeishmania braziliensiskinetoplast DNA, the external control and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extraction control; Multiplex PCR; PUC18; Leishmaniasis; Diagnosis; False-negative result. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200326 |
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| Registros recuperados: 230 | |
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