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Registros recuperados: 62 | |
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Gomes,Fabiane R.; Curcio,Bruna R.; Ladeira,Sílvia R.L.; Fernández,Heriberto; Meireles,Mário Carlos Araújo. |
The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Thermotolerant species; Diarrhea; Chicken. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300032 |
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Alves,Christian D.B.T.; Granados,Oscar F.O.; Budaszewski,Renata da F.; Streck,André F.; Weber,Matheus N.; Cibulski,Samuel P.; Pinto,Luciane D.; Ikuta,Nilo; Canal,Cláudio W.. |
ABSTRACT Although the use of vaccines has controlled enteric diseases in dogs in many developed countries, vaccine coverage is still under optimal situation in Brazil. There is a large population of nonimmunized dogs and few studies about the identification of the viruses associated with diarrhea. To address this situation, stool samples from 325 dogs were analyzed by polymerase chain reaction for the detection of common enteric viruses such as Canine adenovirus (CAdV), Canine coronavirus (CCoV), Canine distemper virus (CDV), Canine rotavirus (CRV) and Carnivorous protoparvovirus 1 (canine parvovirus 2; CPV-2). At least one of these species was detected in 56.6% (184/325) of the samples. The viruses detected most frequently in either diarrheic or... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Distemper; Parvovirus; Diarrhea; Dog; Co-infection. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400790 |
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Stojanovic,Predrag; Kocic,Branislava; Stojanovic,Miodrag; Miljkovic-Selimovic,Biljana; Tasic,Suzana; Miladinovic-Tasic,Natasa; Babic,Tatjana. |
The aim of this study was to fortify the clinical importance and representation of toxigenic and non-toxigenic Clostridium difficile isolated from stool samples of hospitalized patients. This survey included 80 hospitalized patients with diarrhea and positive findings of Clostridium difficile in stool samples, and 100 hospitalized patients with formed stool as a control group. Bacteriological examination of a stool samples was conducted using standard microbiological methods. Stool sample were inoculated directly on nutrient media for bacterial cultivation (blood agar using 5% sheep blood, Endo agar, selective Salmonella Shigella agar, Selenite-F broth, CIN agar and Skirrow's medium), and to selective cycloserine-cefoxitin-fructose agar (CCFA) (Biomedics,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium difficile; Diarrhea; Toxins. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100023 |
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Ud-Din,Abu; Wahid,Syeda. |
Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similarity. Shigella spp. could be easily differentiated from E. coli, but difficulties observed for the EIEC-Shigella differentiation as both show similar biochemical traits and can cause dysentery using the same mode of invasion. Sequencing of multiple housekeeping genes indicates that Shigella has derived on several different occasions via acquisition of the transferable forms of ancestral virulence plasmids... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; E. coli; Shigella; Real-time PCR. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400002 |
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Ferreira,Débora Paula; Silva,Vânia Lúcia; Guimarães,Danielle Aparecida; Coelho,Cíntia Marques; Zauli,Danielle Alves Gomes; Farias,Luiz Macêdo; Carvalho,Maria Auxiliadora Roque; Diniz,Claudio Galuppo. |
Despite the importance of gastrointestinal diseases and their global distribution, affecting millions of individuals around the world, the role and antimicrobial susceptibility patterns of anaerobic bacteria such as those in the Bacteroides fragilis group (BFG) are still unclear in young children. This study investigated the occurrence and distribution of species in the BFG and enterotoxigenic strains in the fecal microbiota of children and their antimicrobial susceptibility patterns. Diarrheic (n=110) and non-diarrheic (n=65) fecal samples from children aged 0-5 years old were evaluated. BFG strains were isolated and identified by conventional biochemical, physiological and molecular approaches. Alternatively, bacteria and enterotoxigenic strains were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteroides fragilis group; ETBF; Antimicrobial susceptibility; Diarrhea. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300010 |
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Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia. |
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015 |
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Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa. |
Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009 |
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Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke. |
Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034 |
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Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.. |
ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003 |
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Cantarelli,Vlademir; Nagayama,Kenichi; Takahashi,Akira; Honda,Takeshi; Cauduro,Paulo F.; Dias,Cícero A.G.; Mezzari,Adelina; Brodt,Teresa. |
We describe the isolation of one strain of Shiga toxin-producing Escherichia coli O91:H21 from a child with diarrhea in the city of Porto Alegre, RS, Brazil. Considering the pathogenic potential of STEC, these organisms should be looked for more carefully among our population. |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterohemorrhagic E. coli; STEC; Enteroaggregative E. coli; Enteropathogenic E. coli; Diarrhea. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400005 |
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Silva,Rodrigo Otávio Silveira; Gabardo,Michelle de Paula; Oliveira,Juliana Saes Vilaça de; Lobato,Francisco Carlos Faria; Guedes,Roberto Maurício Carvalho. |
Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lawsonia intracellularis; Canine; Proliferative enteropathy; Diarrhea. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901619 |
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Pereira,Daniele Araujo; Vidotto,Marilda Carlos; Nascimento,Karla Alvarenga; Santos,Anne Caroline Ramos dos; Mechler,Marina Lopes; Oliveira,Luís Guilherme de. |
ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) is the major cause of diarrhea in newborn and weaned pigs. Bacteria adhesion to the host cell is considered a specific phenomenon among fimbrial and non-fimbrial adhesins with their respective receptors on enterocytes. Enteric disorders are related with the fimbriae F4 (K88), F5 (K99), F6 (987P), F41, and F18. In addition to ETEC, another category of E. coli , porcine pathogenic E. coli (PEPEC),can cause diarrhea in pigs; it produces the porcine attaching and effacing-associated (Paa) adhesin in, which is capable to cause a typical lesion known as an attaching and effacing (A/E) lesion. Immunization of sows with adhesin is important to stimulate the production of antibodies and their subsequent transfer... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colibacillosis; Diarrhea; Vaccines; Swine; Colostrum.. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801430 |
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Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan. |
ABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451 |
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Bregadioli,Gabriela de Castro; Pereira,Priscilla Fajardo Valente; Flaiban,Karina Keller Marques da Costa; Ribeiro Filho,José Dantas; Lisbôa,Júlio Augusto Naylor. |
ABSTRACT: According to the World Health Organization, the development of orally replacement fluids and electrolytes was one of the most significant advances of the twentieth century, markedly reducing mortality from diarrheal children. In veterinary medicine, oral electrolyte solutions (OES) are routinely used to treat diarrheic neonatal calves in order to correct water, electrolyte and acid base imbalances. In North America, the use of OES is routine and there are at least 20 commercial alternatives. Use of OES has increased in recent years in Brazil, but just five commercial products are currently available. The OES should contain, in suitable concentrations, electrolytes, glucose and an alkalizing agent to ensure its therapeutic efficacy. This review... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electrolyte imbalance; Diarrhea; Oral electrolyte solution; Dehydration; Acid base imbalance. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700603 |
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Gonçalves,Liliane Maria Piano; Kiefer,Charles; Silva,Camilla Mendonça; Leal,Cássia Rejane Brito; Alencar,Stephan Alexander da Silva; Carvalho,Kelly Cristina Nunes; Rufino,Luciana Moura. |
ABSTRACT: This study was conducted to assess the use of propolis ethanolic extract as an alternative to performance-enhancing antibiotics for piglets. Seventy piglets weaned at 21 days of age, with initial weights of 7.3±0.4kg and final weights of 24.9±1.2kg, were randomly assigned to five diets: positive control (diet with 200ppm antibiotic); negative control (diet without growth promoter); and diets with 100, 500, and 1,000ppm of brown propolis ethanolic extract, with seven replicates of two piglets each. The duration of the experimental period was 35 days. The diets did not influence (P>0.05) daily feed intake, daily weight gain, final weight, and feed conversion of animals. The bacteriological profile of the nasal swab presented a predominance of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobials; Diarrhea; Ethanolic extract; Vegetable extract. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000100702 |
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Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria. |
Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027 |
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Registros recuperados: 62 | |
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