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First evaluation of resistance to both a California OsHV-1 variant and a French OsHV-1 microvariant in Pacific oysters ArchiMer
Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Dégremont, Lionel; Burge, Colleen A.; Mancilla Cortez, Daniel; Friedman, Carolyn S.; Fleener, Gary B.; Dumbauld, Brett R.; Langdon, Chris.
Background Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in...
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Crassostrea gigas; Heritability; Breeding.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/70974/69206.pdf
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Minimizing maternal effect in salmonid families mixed since eyed stages and a posteriori DNA-pedigreed ArchiMer
Haffray, Pierrick; Vandeputte, Marc; Petit, V.; Pincent, Cedric; Chatain, Beatrice; Chapuis, H.; Meriaux, J. C.; Coudurier, B.; Quillet, E.; Dupont-nivet, M..
Application of DNA parentage assignment for commercial selection in aquaculture is still rare. An experiment was performed to quantify and to minimize maternal effects on growth in rainbow trout. Six hundred families were reared until 198 day post fertilization (dpf) according to two different procedures. In the first procedure (NORM), all families were pooled at eyed stage. In the second procedure (MIN), where maternal effects due to differences in egg size were expected to be minimal, the spawns were divided into sub-groups with similar mean egg weight at eyed stage. These sub-groups were then pooled when they achieved the same mean body length (147 dpf). Genetic parameters were estimated for body weight, body length and condition factor at 198 dpf, with...
Tipo: Text Palavras-chave: Rainbow trout; Fingerprint; Maternal effect; Heritability; Growth; Triploid.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00115/22639/20395.pdf
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Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield ArchiMer
Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Response to divergent selection for "high" and "low" survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer mortality was assessed in three sites along French coasts, to determine their response to selection and estimate realized heritability of the trait. A significant difference in survival was observed between the "high" and "low" selected groups at all sites for all sets of progenies, indicating a significant genetic component. High realized heritabilities for survival...
Tipo: Text Palavras-chave: Crassostrea gigas; Heritability; Juveniles; Summer mortality; Survival; Growth.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2009/publication-6950.pdf
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Estimation of Genetic Parameters of Fatty Acids Composition in Flesh of Market Size Common Carp (Cyprinus carpio L.) and Their Relation to Performance Traits Revealed that Selective Breeding Can Indirectly Affect Flesh Quality ArchiMer
Prchal, Martin; Vandeputte, Marc; Gela, David; Dolezal, Marek; Blichtova, Hana; Rodina, Marek; Flaishans, Martin; Kocour, Martin.
Fish are a rich source of omega-3 polyunsaturated fatty acids (n-3 PUFAs) and thus, they should be an integral part of human diet at least twice a week. As a result, high attention has been devoted to the improvement of fatty acids (FA) content in the flesh of farmed fish through nutrition. Conversely, there are very few data on the potential of selective breeding to improve FA composition in fish. We estimated genetic parameters of fillet fatty acid content and performance traits in market size common carp cultured under semi-intensive pond conditions. The experimental stock arose through factorial mating of 7 dams and 36 sires. All families were reared communally. Pedigree was reconstructed with microsatellite markers, and 158 individuals were dressed...
Tipo: Text Palavras-chave: Genetic correlations; Genetic improvement; Growth; Heritability; Slaughtering traits; Supplemental feeding.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56043/57558.pdf
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Quantifying the genetic parameters of feed efficiency in juvenile Nile tilapia Oreochromis niloticus ArchiMer
De Verdal, Hugues; Vandeputte, Marc; Mekkawy, Wagdy; Chatain, Beatrice; Benzie, John A. H..
Background: Improving feed efficiency in fish is crucial at the economic, social and environmental levels with respect to developing a more sustainable aquaculture. The important contribution of genetic improvement to achieve this goal has been hampered by the lack of accurate basic information on the genetic parameters of feed efficiency in fish. We used video assessment of feed intake on individual fish reared in groups to estimate the genetic parameters of six growth traits, feed intake, feed conversion ratio (FCR) and residual feed intake in 40 pedigreed families of the GIFT strain of Nile tilapia, Oreochromis niloticus. Feed intake and growth were measured on juvenile fish (22.4g mean body weight) during 13 consecutive meals, representing 7days of...
Tipo: Text Palavras-chave: Feed conversion ratio; Tilapia; Heritability; Genetic estimations; Correlations.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00469/58115/60551.pdf
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Crossing phenotype heritability and candidate gene expression in grafted black-lipped pearl oyster Pinctada margaritifera, an animal chimera ArchiMer
Blay, Carole; Planes, Serge; Ky, Chin-long.
Grafting mantle tissue of a donor pearl oyster into the gonad of a recipient oyster results in the formation of a chimera, the pearl sac. The phenotypic variations of this chimera are hypothesized to be the result of interactions between the donor and recipient genomes. In this study, the heritability of phenotypic variation and its association with gene expression were investigated for the first time during P. margaritifera pearl production. Genetic variance was evaluated at different levels, 1) before the graft operation (expression in graft tissue), 2) after grafting (pearl sac tissue expression in chimera) and 3) on the product of the graft (pearl phenotype traits) based on controlled bi-parental crosses and the F1 generation. Donor related genetic...
Tipo: Text Palavras-chave: Gene expression; Heritability; Pearl oyster; Phenotype; Pinctada margaritifera.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00433/54505/55876.pdf
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How could fully scaled carps appear in natural waters in Madagascar? ArchiMer
Hubert, Jean-noel; Allal, Francois; Hervet, Caroline; Ravakarivelo, Monique; Jeney, Zsigmond; Vergnet, Alain; Guyomard, Rene; Vandeputte, Marc.
The capacity of organisms to rapidly evolve in response to environmental changes is a key feature of evolution, and studying mutation compensation is a way to evaluate whether alternative routes of evolution are possible or not. Common carps (Cyprinus carpio) carrying a homozygous loss-of-function mutation for the scale cover gene fgfr1a1, causing the 'mirror' reduced scale cover, were introduced in Madagascar a century ago. Here we show that carps in Malagasy natural waters are now predominantly covered with scales, though they still all carry the homozygous mutation. We also reveal that the number of scales in mutated carps is under strong polygenic genetic control, with a heritability of 0.49. As a whole, our results suggest that carps submitted to...
Tipo: Text Palavras-chave: Evolution; Mutation compensation; Heritability.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00356/46681/46725.pdf
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Estimates of heritability and genotype-environment interactions for body weight in sea bass (Dicentrarchus labrax L.) raised under communal rearing conditions ArchiMer
Saillant, Eric; Dupont Nivet, Mathilde; Haffray, Pierrick; Chatain, Beatrice.
Genetic parameters for somatic growth rate in sea bass were estimated in two experiments where 27 (year-class 1997) to 30 (year-class 1998) families of sea bass (3 dams mated with 9-10 sires according to a full factorial design) were raised mixed in the same tanks starting shortly before hatching (48 h post fertilization). Trials were performed under two temperature (experiment 1, 1997: group high temperature (HT) and group low temperature (LT)) and two density regimes (experiment 2, 1998: group high density (HD) and group low density (LD)) with a subset of the mating design (18 families) being reproduced in the two experiments. All the fish were genotyped at 3-6 microsatellite loci which allowed them to be assigned to the individual breeders used in the...
Tipo: Text Palavras-chave: Genotype x environment interaction; Communal rearing; Microsatellites markers; Dicentrarchus labrax; Growth; Heritability.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1706.pdf
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Response to selection for increasing resistance to the spring mortality outbreaks in Mytilus edulis occurring in France since 2014 ArchiMer
Degremont, Lionel; Maurouard, Elise; Rabiller, Manuella; Glize, Philippe.
Massive spring mortality outbreaks have been reported in Mytilus spp. in France since 2014. The main areas affected are the Pertuis Charentais Sounds and the coast of the Pays de la Loire, which cultivate mainly M. edulis, and the putative causal agents remain unknown. We report the results of the first generation of mass selection focused on survival and resistance to the spring mortality in M. edulis. Two stocks of mussels were sampled in the Pertuis Charentais Sounds in 2014; one of these went through a spring mortality outbreak and the survivors were used to produce the selected stock, while the second stock was M. edulis considered to be naïve against the putative causal agents of the mortality outbreak and was used to produce the control stock. A...
Tipo: Text Palavras-chave: Spring mortality; Survival; Mytilus edulis; Heritability; Selection.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61619/65530.pdf
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Genetic variation of resistance to Viral Nervous Necrosis and genetic correlations with production traits in wild populations of the European sea bass ( Dicentrarchus labrax ) ArchiMer
Khanh, Doan Quoc; Vandeputte, Marc; Chatain, Beatrice; Haffray, Pierrick; Vergnet, Alain; Breuil, Gilles; Allal, Francois.
Viral Nervous Necrosis (VNN) disease is considered as one of the most serious threats for European sea bass cultured in Mediterranean Sea, with no simple and effective procedures to treat this disease. In this study, 1472 offspring resulting from artificial full factorial mating of western Mediterranean dams with sires from four different wild populations of European sea bass (Northern Atlantic, NAT; Western Mediterranean, WEM; Northern-East Mediterranean, NEM; and Southern-East Mediterranean, SEM) were challenged by experimental infection to W80 betanodavirus strain in order to evaluate genetic variations for VNN resistance among populations and genetic correlations between VNN resistance and production traits. The results showed a large variation of VNN...
Tipo: Text Palavras-chave: VNN disease; VNN resistance; Heritability; Genetic correlation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00385/49612/50124.pdf
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Quantitative genetic variation for post-stress cortisol and swimming performance in growth-selected and control populations of European sea bass (Dicentrarchus labrax) ArchiMer
Vandeputte, Marc; Porte, J. D.; Auperin, B.; Dupont-nivet, M.; Vergnet, Alain; Valotaire, C.; Claireaux, Guy; Prunet, P.; Chatain, Beatrice.
Sea bass is a major species in Mediterranean aquaculture, and is now being subject to selective breeding programs for faster growth. In terrestrial species, it was demonstrated that fast growth may be linked to a correlated degradation of fitness traits. In this experiment, we evaluated 600 young sea bass from a factorial mating of 76 sires and 13 dams. The sires were from four genetic groups, wild (W), domesticated (D), and selected for growth (2 groups, M and P). The 600 offspring were submitted to two acute confinement stress challenges at 6 weeks intervals, and plasma cortisol at one hour post stress was measured. The same fish were also submitted to two swimming challenges at a 5 days interval, where the maximum sustained swimming speed (Umax) of each...
Tipo: Text Palavras-chave: Aquaculture; Selective breeding; Stress response; Maximum sustained swimming speed; Heritability; Correlated response.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00308/41955/41218.pdf
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Family effect on cultured pearl quality in black-lipped pearl oyster Pinctada margaritifera and insights for genetic improvement ArchiMer
Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe.
Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of...
Tipo: Text Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf
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In search for indirect criteria to improve feed utilization efficiency in sea bass (Dicentrarchus labrax) Part II: Heritability of weight loss during feed deprivation and weight gain during re-feeding periods ArchiMer
Grima, Laure; Chatain, Beatrice; Ruelle, Francois; Vergnet, Alain; Launay, Amandine; Mambrini, Muriel; Vandeputte, Marc.
Selective breeding to improve residual feed intake (RFI) in sea bass (Dicentrarchus labrax) is a major goal that would optimize economic gain while minimizing the environmental impact of production. Due to the difficulty in accurately measuring individual feed intake, no selective breeding program has yet been started. In a previous study, we identified a criterion phenotypically related to RFI variations: the loss of weight during feed deprivation, FD. Moreover, an additional composite criterion (CC) integrating both FD and weight gain during subsequent re-feeding (RF) was closely related to RFI, even though the relationship was only close to significance (P=0.06). The aim of the present study was to estimate the heritability of these two traits, in order...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Feed efficiency; Heritability; Genetic parameters; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11529/8213.pdf
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Investigation of morphological predictors of fillet and carcass yield in European sea bass (Dicentrarchus labrax) for application in selective breeding ArchiMer
Vandeputte, Marc; Puledda, Antonio; Tyran, Anne Sophie; Bestin, Anastasia; Coulombet, Celine; Bajek, Aline; Baldit, Gwenaelle; Vergnet, Alain; Allal, Francois; Bugeon, Jerome; Haffray, Pierrick.
Genetic parameters for carcass and fillet percentage were estimated in 760 European sea bass reared under commercial conditions and slaughtered at 573 days post fertilization (395 g mean body weight). Phenotyped fish were the offspring of 45 sires and 20 dams crossed in a factorial mating design. Pedigrees were re-constructed with 90.7% success using 12 microsatellites. The heritability of fillet yield was moderately low (0.21), while it was high for carcass yield (0.57). Both traits were poorly correlated (− 0.01 to 0.28) making space for their combined improvement. We investigated different predictors derived from measurement of surfaces on digital pictures and ultrasound measurements at several points of the body. The accuracy of the phenotypic...
Tipo: Text Palavras-chave: Ultrasound tomography; Genetics; Heritability; Indirect selection; Processing yields.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00361/47217/47173.pdf
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Genotype by environment interactions for growth in European seabass (Dicentrarchus labrax) are large when growth rate rather than weight is considered ArchiMer
Dupont-nivet, Mathilde; Karahan-nomm, Bilge; Vergnet, Alain; Merdy, Olivier; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice; Vandeputte, Marc.
Two hundred fifty three full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank for 14 months until reaching mean weight of 35 g. then 7000 of them were individually tagged and weighed, and dispatched to four farms in different locations (France, Israel, Italy and Portugal) representing a wide variety of environmental conditions. Around mean weight of 400 g. 1177 to 1667 fish at each site were weighed. Daily growth coefficient (DGC) was calculated. Pedigrees were successfully redrawn for 99.2% of fish using microsatellite markers. Genetic correlations between sites were high for body weight (>0.80 in all cases but one, i.e., five cases over six), but only...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Seabass; Growth; Daily growth coefficient; Heritability; G x E interactions; Aquaculture.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12256/9227.pdf
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Genetic variation for growth at one and two summers of age in the common carp (Cyprinus carpio L.): Heritability estimates and response to selection ArchiMer
Vandeputte, Marc; Kocour, M; Mauger, S; Rodina, M; Launay, A; Gela, D; Dupont Nivet, M; Hulak, M; Linhart, O.
We estimated the heritability of growth-related traits (weight and length at ages one summer, first spring and two summers) in a synthetic mirror carp strain (HSM) in the Czech Republic. The four generation pedigree was obtained from parentage assignment of three factorial mating designs with microsatellite markers, and included 195 fish without phenotypes (48 G0, 147 G1) and 1321 fish with phenotypes (674 G2, 647 G3). Animal model heritability estimates over generations were in the range 0.21-0.33 for length and in the range 0.31-0.44 for weight. The genetic correlation between length and weight was high (0.97). The correlations between growth measurements in the first and in the second summer of age were moderate to low (0.34-0.67). Divergent selection...
Tipo: Text Palavras-chave: Quantitative genetics; Performance; Microsatellites; Parentage assignment; Realized heritability; Selection response; Selection; Growth; Heritability; Cyprinus carpio; Common carp.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4076.pdf
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Evolution of morphological but not aggressiveness‐related traits following a major resistance breakdown in the poplar rust fungus, Melampsora larici‐populina ArchiMer
Maupetit, Agathe; Fabre, Bénédicte; Pétrowski, Jérémy; Andrieux, Axelle; De Mita, Stéphane; Frey, Pascal; Halkett, Fabien; Hayden, Katherine.
Crop varieties carrying qualitative resistance to targeted pathogens lead to strong selection pressure on parasites, often resulting in resistance breakdown. It is well known that qualitative resistance breakdowns modify pathogen population structure but few studies have analysed the consequences on their quantitative aggressiveness‐related traits. The aim of this study was to characterize the evolution of these traits following a resistance breakdown in the poplar rust fungus, Melampsora larici‐populina. We based our experiment on three temporal populations sampled just before the breakdown event, immediately after and four years later. First, we quantified phenotypic differences among populations for a set of aggressiveness traits on a universally...
Tipo: Text Palavras-chave: Disease-associated traits; Heritability; Mixed model; Plant pathogen; Q(ST)-F(ST)comparisons; Temporal sampling.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00652/76396/77420.pdf
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Genetic parameters of in-vivo prediction of carcass, head and fillet yields by internal ultrasound and 2D external imagery in large rainbow trout (Oncorhynchus mykiss) ArchiMer
Haffray, Pierrick; Bugeon, Jerome; Rivard, Quentin; Quittet, Benjamin; Puyo, Sophie; Allamelou, Jean Michel; Vandeputte, Marc; Dupont-nivet, Mathilde.
Selection to improve processing yields relies on sib selection, in which live candidates are ranked according to their family breeding value. This approach limits genetic progress, as it only exploits genetic variability between families and not within them. Indirect criteria measured on live candidates could overcome this limitation. The present study (1) proposes a procedure to identify indirect criteria to predict processing yields in rainbow trout (head, carcass and fillet yields), (2) estimates genetic parameters of these indirect criteria, and (3) predicts relative genetic gains in processing yields using full-sib selection or indirect individual selection on those indirect criteria. DNA-pedigreed all-female rainbow trout Oncorhynchus mykiss (n =...
Tipo: Text Palavras-chave: Salmonids; Heritability; Fillet yield; Selection; Aquaculture; Morphometry.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26847/25249.pdf
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First Evidence of Realized Selection Response on Fillet Yield in Rainbow Trout Oncorhynchus mykiss, Using Sib Selection or Based on Correlated Ultrasound Measurements ArchiMer
Vandeputte, Marc; Bugeon, Jérôme; Bestin, Anastasia; Desgranges, Alexandre; Allamellou, Jean-michel; Tyran, Anne-sophie; Allal, Francois; Dupont-nivet, Mathilde; Haffray, Pierrick.
Fillet yield, the proportion of edible fillet relative to body weight, is a major trait to improve in fish sold processed, as it has a direct impact on profitability and can simultaneously decrease the environmental impact of producing a given amount of fillet. However, it is difficult to improve by selective breeding, because it cannot be measured on live breeding candidates, its phenotypic variation is low, and, as a ratio, it is not normally distributed and a same change in fillet yield can be the result of different changes in fillet weight and body weight. Residual headless gutted carcass weight (rHGCW) is heritable and highly genetically correlated to Fillet% in rainbow trout, and can be predicted by the ratio of abdominal wall thickness to depth of...
Tipo: Text Palavras-chave: Aquaculture; Fillet yield; Selective breeding; Selection response; Production efficiency; Heritability.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00599/71121/69423.pdf
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Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). I. Estimation of genetic parameters for survival and growth ArchiMer
Degremont, Lionel; Ernande, Bruno; Bedier, Edouard; Boudry, Pierre.
The multidisciplinary project "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As pail of the MOREST project, 43 full-sib families nested within 17 half-sib families were produced, planted out and tested in 3 sites during summer 2001 to assess to what extent genetic variability exists for this trait. A strong genetic basis was found for survival as narrow-sense heritability estimates ranged from 0.47 to 1.08, with higher values in sites where summer mortality was higher. Genetic correlations across sites were positive and very high for survival, indicating no genotype by environment interaction. In contrast, lower genetic variation was observed...
Tipo: Text Palavras-chave: Growth; Survival; Juveniles; Heritability; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2457.pdf
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