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| Registros recuperados: 58 | |
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| Costa,Fernanda Flores; Lima,Helena Regina Pinto; Cunha,Maura Da; Santos,Inês da Silva. |
| The Monimiaceae is represented in the Neotropical region by six genera. Macropeplus Perkins is a Brazilian genus comprising four species restricted to high-montane Atlantic Forest areas, which have not been properly examined from the anatomical point of view. This study characterizes the anatomical structure and histochemical aspects of the leaves of Macropeplus dentatus and M. ligustrinus, and adds to our taxonomic knowledge ofthe genus. The individuals studied were collected in the Serra dos Órgãos National Park and in the Caraça Natural Park in southeastern Brazil. Among the characteristics common to the two species, hypostomatic leaves, dorsiventral structure, paracytic stomata, a subepidermal layer, collateral vascular system in the form of an arc in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atlantic Rain Forest; Histochemistry; Leaf anatomy; Macropeplus; Monimiaceae. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000300027 |
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| Soares,Taliane Leila; Jesus,Onildo Nunes de; Souza,Everton Hilo de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de. |
| The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro germination; Histochemistry; Scanning electron microscopy; Passifloraceae; Pollen tube. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018 |
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| Abud, Haynna Fernandes; Araújo, Eduardo Fontes; Araujo, Roberto Fontes; Picoli, Edgard Augusto de Toledo; Gallão, Maria Izabel. |
| This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol’s iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum frutescens; C. chinense; Chemical composition; Histochemistry; Reserves.. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32492 |
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| GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C.. |
| This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973 |
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| FERNANDES,YANNE S.; TRINDADE,LUMA M.P.; REZENDE,MARIA HELENA; PAULA,JOSÉ R.; GONÇALVES,LETÍCIA A.. |
| Trichogonia cinerea is endemic to Brazil and occurs in areas of cerrado and campo rupestre. In this study, we characterized the glandular and non-glandular trichomes on the aerial parts of this species, determined the principal events in the development of the former, and identified the main constituents of the volatile oil produced in its aerial organs. Fully expanded leaves, internodes, florets, involucral bracts, and stem apices were used for the characterization of trichomes. Leaves, internodes, florets, and involucral bracts were examined by light microscopy and scanning electron microscopy, whereas stem apices were examined only by light microscopy. Branches in the reproductive phase were used for the extraction and determination of the composition... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Volatile oil; Histochemistry; Ontogeny; Secretory structure. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100309 |
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| SOUZA,DOUGLAS M.F. DE; SÁ,RAFAELA D.; ARAÚJO,EVANI L.; RANDAU,KARINA P.. |
| ABSTRACT Solidago chilensis Meyen, belonging to the family Asteraceae, is a plant native to South America and the only representative of the genus in Brazil. This species is popularly known as “arnica” and is used to treat bruises, muscle pain and inflammation. The objective of this work was to contribute to the pharmacognostic standardization of S. chilensis. Cross-sections were obtained, by freehand, for microscopic analysis of root, stem and leaf; for these parts of the plant maceration was also performed according to the method of Jeffrey. For the leaf were still made paradermal sections, scanning electron microscopy analysis, phytochemical and histochemical tests. Thus, it was determined anatomical features useful for diagnosis of the species which,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anatomy; Arnica; Asteraceae; Histochemistry; Phytochemistry; Solidago chilensis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502107 |
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| Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie. |
| The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p<0.05). Microscopically, C and T groups showed polygonal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003 |
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| oic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M.. |
| The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008 |
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| Rigon,F.; Rossato,D.; Auler,V.B.; Dal Bosco,L.; Faccioni-Heuser,M.C.; Partata,W.A.. |
| Frogs have been used as an alternative model to study pain mechanisms. Since we did not find any reports on the effects of sciatic nerve transection (SNT) on the ultrastructure and pattern of metabolic substances in frog dorsal root ganglion (DRG) cells, in the present study, 18 adult male frogs (Rana catesbeiana) were divided into three experimental groups: naive (frogs not subjected to surgical manipulation), sham (frogs in which all surgical procedures to expose the sciatic nerve were used except transection of the nerve), and SNT (frogs in which the sciatic nerve was exposed and transected). After 3 days, the bilateral DRG of the sciatic nerve was collected and used for transmission electron microscopy. Immunohistochemistry was used to detect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Axotomy; Immunoreactivity; Histochemistry. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600513 |
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| The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations. Neuronal density was 1,500 ± 116 neurons/cm2 (mean ± SEM) in the esophagus, 8,900 ± 1,518 in the stomach, 9,000 ± 711 in the jejunum and 13,100 ± 2,089 in the colon. The difference in neuronal density between the esophagus and other regions was statistically significant. The neuron profile area ranged from 45 to 1,100 µm2. The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Myenteric plexus; Wild mouse; Calomys callosus; Morphometry; Histochemistry. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500009 |
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| Souza,N.B.; Liberti,E.A.; De-Souza,R.R.. |
| The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (mean ± SD) 7,488 ± 293 neurons/cm2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Submucous plexus; Wild mouse; Morphometry; Histochemistry; Ultrastructure; Calomys callosus. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500007 |
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| Moura,Bruna Izabela de Vasconcelos; Araújo,Bárbara Priscylla Lira de; Sá,Rafaela Damasceno; Randau,Karina Perrelli. |
| Manilkara zapota (L.) P. Royen, popularly known as sapoti or sapota (sapodilla), is a tree bearing an important fruit, in addition to different parts of the plant being widely used in folk medicine in the management of inflammation, pain, fevers, coughs, diarrhea, dysentery, among other ailments. This study aimed to conduct a pharmacobotany standardization study of M. zapota. Semi-permanent slides, containing transversal sections of stem, petiole, leaf blade and fruit; and paradermic sections of leaf blade were prepared, and analyzed by light microscopy. Histochemical tests were also performed in cross-sections of the leaf blade. Microscopic analysis allowed the identification of important elements in the diagnosis of the species; while the use of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anatomy; Histochemistry; Sapota; Sapoti; Manilkara/drug effects; Sapotaceae/drug effects.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100547 |
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| Registros recuperados: 58 | |
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