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Anaesthesia and gonad sampling in the European flat oyster (Ostrea edulis) ArchiMer
Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene.
Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf
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Rapport sur l'activité du laboratoire de La Tremblade - Année 1960 ArchiMer
Trochon, Pierre.
Rapport sur l'activité du Laboratoire ISTPM (Institut Scientifique et Technique des Pêches Maritimes) de La Tremblade année 1960
Tipo: Text Palavras-chave: Rapport activité; Conchyliculture; Hydrologie; Mortalité; Météorologie; Reproduction; Claire ostréicole; Crassostrea angulata; Ostrea edulis; Mytilus edulis; Marennes Oléron.
Ano: 1960 URL: http://archimer.ifremer.fr/doc/00069/18054/15596.pdf
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Etude ecophysiologique de quelques mollusques bivalves ArchiMer
Riva, A; Masse, H.
Using an experimental flowing system, the filtration rate of Mytilus galloprovincialis, Ostrea edulis, Ruditapes decussatus and Ruditapes aureus has been measured by an indirect technique under Mediterranean thermal conditions in coastal open sea. The oxygen consumption and excretion rate of ammonia have been estimated and influence of temperature and salinity tested for M. galloprovincialis . The results are discussed and compared to literature data.
Tipo: Text Palavras-chave: Ruditapes decussatus; Ostrea edulis; Mytilus galloprovincialis; Bivalvia; Respiration; Excretion; Oxygen consumption; Water filtration; Ecophysiology; Méditerranéee; Excrétion; Respiration; Filtration; Bivalves.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1177.pdf
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Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro ArchiMer
Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf
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Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph ArchiMer
Hubert, Florence; Van Der Knaap, Wil; Noel, Thierry; Roch, Philippe.
Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) and protozoan cells. Procaryotes (Escherichia coli and Vibrio alginolyticus) were not sensitive to the cytotoxicity. The activity was still present in dialyzed samples but was inhibited by heating at 45°C. Large individual variability which was not correlated with protein concentration and an increasing number of reactive specimens following injection, suggested that naive mussels were in various stages of immune response. Purification by anion exchange chromatography followed by gel filtration revealed a 320 kDa cytotoxic polymeric protein that acts through a polymerization process after binding onto target cell membranes as...
Tipo: Text Palavras-chave: Cytotoxicité; Antibactérien; Hémolymphe; Lysat d'hémocyte; Moule; Huître; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Cytotoxicity; Antibacterial; Hemolymph; Hemocyte lysate; Musssel; Oyster; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00039/15008/12333.pdf
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Serological and analysis of Bonamia in Ostrea edulis and Tiostrea lutaria using polyclonal and monoclonal antibodies ArchiMer
Mialhe, Eric; Boulo, Viviane; Elston, Ralph; Hill, Barry; Hine, Michel; Montes, Jaime; Mont, Jaime; Banning, Paul Van; Grizel, Henri.
Murine moncolonal and polyclonal antibodies to antigesn of Bonamia ostreae were assessed for serological comparaison of parasites associated with different geographical originns of flat oyster, Ostrea edulis, and with Tiostrea lutaria. All Bonamia isolates from O. edulis reacted similary with polyclonal and monoclonal antibodies. The parasite associed with Tiostrea lutaria which lacks reactivity with 20 B 2 MAB must be considered as a serologically distinct from of B. ostreae
Tipo: Text Palavras-chave: Serological comparison; Polyclonal and monoclonal antibodies; Tiostrea lutaria; Ostrea edulis; Bonamia; Comparaison sérologique; Anticorps polyclonaux et monoclonaux; Tiostrea lutaria; Ostrea edulis; Bonamia.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3101.pdf
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Population bottleneck and effective size in Bonamia ostreae-resistant populations of Ostrea edulis as inferred by microsatellite markers ArchiMer
Launey, Sophie; Barre, Marc; Gerard, Andre; Naciri, Yamama.
Genetic variability at five microsatellite loci was analysed in three hatchery-propagated populations of the flat oyster, Ostrea edulis. These populations were part of a selection programme for resistance to the protozoan parasite Bonamia ostreae and were produced by mass spawns, without control of the genealogy. Evidence for population bottlenecks and inbreeding was sought. A reduction in the number of alleles, mainly due to the loss of rare alleles, was observed in all selected populations, relative to the natural population from which they were derived. Heterozygote excesses were observed in two populations, and were attributed to substructuring of the population into a small number of families. Pedigree reconstruction showed that these two populations...
Tipo: Text Palavras-chave: Inbreeding; Effective population size; Bonamia ostreae; Ostrea edulis; European flat oyster.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-2703.pdf
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Relance de l'huître plate, Ostrea edulis. Bilan des travaux réalisés en 1993 ArchiMer
Martin, Anne-genevieve.
A revival program for the European flat oyster Ostrea edulis was initiated in Brittany in 1984 and funded by regional and national agencies. Since then, the research programs were carried out and the 1993 results are presented in this report. Disease status and spat settlement trends are described within each breeding area as weil as advances in genetic research.
Tipo: Text Palavras-chave: Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiologie; Pathologie; Génétique; Echantillonnage; Elevage; Bivalves; Ostrea edulis; Bonamia ostreae; Marteilia refringens; Epidemiology; Pathology; Genetic; Sampling; Breeding.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00427/53822/54813.pdf
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Genetic diversity of the European oyster (Ostrea edulis L.) in Nova Scotia: Comparison with other parts of Canada, Maine and Europe and implications for broodstock management ArchiMer
Vercaemer, Bénédikte; Spence, Koren R.; Herbinger, Christophe; Lapegue, Sylvie; Kenchington, Ellen L..
The European oyster (Ostrea edulis) was introduced to the Nova Scotia aquaculture industry 30 years ago using stocks imported from naturalized populations in Maine whose ancestors originated in the Netherlands. This study used 5 microsatellites to assess the level of genetic diversity in several hatchery stocks and naturalized populations from Nova Scotia, New Brunswick, British Columbia and Maine. Some genetic erosion was shown to have occurred in the Maritimes populations, with the largest loss of alleles being experienced by the hatchery stocks. In spite of this loss, genetic diversity and heterozygosity in the Maritimes populations are still relatively high. Relationships within and between the populations and the existence of kin groups within the...
Tipo: Text Palavras-chave: Genetic diversity; Maine; Microsatellites; Flat oyster; European oyster; Ostrea edulis.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2121.pdf
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A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf
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Extension de la maladie de la glande digestive de l'huître plate en Bretagne ArchiMer
Tige, Gilbert; Morel, Marc.
Depuis plusieurs années, l'huître plate O. edulis, cultivée en France est atteinte d'une maladie de la glande digestive. Jusqu'à la fin de l'été 1973, les élevages de l'Aber Benoît, de l'Aber Wrach et de la Penzé étaient les seuls qui, en Bretagne, fussent parasités (Grizel et Tigé, 1973). Depuis, la situation a très nettement évolué. A la fin de l'année 1973, l'épizootie a gagné les secteurs ostréicoles de Morlaix et d'une partie de la rade de Brest ; elle s'est étendue d'avril à juin 1974 à la quasi totalité des autres centres d'élevage. Nous rapporterons ici les observations faites depuis 1973.
Tipo: Text Palavras-chave: Pathologie; Huître plate; Ostrea edulis; Glande digestive; Bretagne.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/00015/12628/9516.pdf
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Etude de la dynamique des populations zooplanctoniques au sein d'une claire ostréicole ArchiMer
Sajus, Marie-celine.
Marteilia refringens est un protozoaire parasite de l'huître plate Ostrea edulis, à l'origine de mortalités dramatiques en élevage depuis les années 1970. En zone endémique, le seul moyen de contrôle de la marteiliose, est une gestion du risque de contamination. Dans ce cadre, la connaissance du cycle parasitaire de Marteilia refringens est primordiale. Les travaux réalisés à ce sujet ont permis de poser l'hypothèse d'un cycle hétéroxène. Le parasite a été détecté par PCR puis localisé au sein des tissus par hybridation in situ au sein d'une espèce de copépode Calanoide prélevée dans une claire ostréicole. A ce stade, cette espèces peut être considéré comme un hôte de Marteilia refringens. Notre étude consistait à mettre en évidence la dynamique des...
Tipo: Text Palavras-chave: Marteilia refringens; Parasitologie; Huître plate; Ostrea edulis; Eau de mer; Zooplancton; Claires ostréicoles.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00032/14351/11635.pdf
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Elevages de bivalves à partir de naissain d'écloseries dans les claires du bassin de Marennes-Oléron ArchiMer
Gras, Paul; Gras, Marie-paule.
Des élevages expérimentaux d'huîtres plates Ostrea edulis et de palourdes Ruditapes decussatus et Ruditapes philippinarum ont été réalisés dans les claires à huîtres du bassin de Marennes-Oléron à partir de naissain provenant d'écloserie. Les croissances pondérale et linéraire et le pourcentage de mortalité ont été étudiés. Les premiers résultats obtenus sont encourageants, et, plusieurs ostréiculteurs ont commencé, cette année, avec notre assistance, la culture des palourdes dans leurs claires à huîtres.
Tipo: Text Palavras-chave: Ostréiculture; Huître; Palourde; Ostrea edulis; Ruditapes decussatus; Ruditapes philippinarum; Claire ostréicole; Marennes Oléron; Charente Maritime.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00009/12010/8702.pdf
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Optimisation de la méthode de quantification de l'infection à Bonamia ostreae chez l'huître plate Ostrea edulis par P.C.R. quantitative ArchiMer
Dupin, Clément.
Le Laboratoire de Génétique et de Pathologie a développé un programme de recherche concernant la bonamiose, maladie due au protiste Bonamia ostreae. Le diagnostic de la maladie repose essentiellement sur deux techniques (histologie et la cytologie par apposition de tissus cardiaque), cependant la sensibilité de ces technique reste relativement faible. C'est pourquoi il est nécessaire de mettre au point une technique plus sensible, afin de pouvoir établir un diagnostic plus précoce de la maladie. Notre étude a porté sur le développement et la mise au point de la méthode de PCR quantitative sur une portion du gène de l'actine I de Bonamia ostreae récemment caractérisé. Les résultats obtenus ont permis de sélectionner le couple d'amorces les plus efficace...
Tipo: Text Palavras-chave: Bonamia ostreae; Ostrea edulis; PCR-Q; Clonage; Prémix Brillant (R); Réaction de polymérisation en chaîne (P.C.R.); Extraction d'ADN; Référence dye; Quantification absolue.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00368/47913/47942.pdf
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Croissance et indice de condition de l'huître plate Ostrea edulis élevée en mer et en étang sur la côte méditerranéenne ArchiMer
Paquotte, Philippe; Moriceau, Jacques.
A monitoring of the growth and condition index of the flat oyster Ostrea edulis has been conducted in the Thau Lagoon and in open sea off the Cap d'Agde. Between April and November, its growth is greater in open sea than in the lagoon. In winter, the growth doesn't stop in open sea, but one needs to get away from mussel collection in practicing seabed farming. In these conditions, the oyster's growth, throughout the year, is very close in open sea and in lagoons whereas the condition index is higher in open sea.
Tipo: Text Palavras-chave: Méditerranée; Mer ouverte; Etang de Thau; Indice de condition; Croissance; Ostrea edulis.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-6188.PDF
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Influence de la température sur la filtration et la consommation d'oxygène de l'huître plate Ostrea edulis: détermination des coefficients allométriques ArchiMer
Haure, Joel; Penisson, Christian; Bougrier, Serge; Baud, Jean-pierre.
La filtration et la consommation d'oxygène d'Ostrea edulis ont été mesurées à différentes températures (10°-30°C) sur des individus de 5 à 120g de poids total (0,1 à 2,7g de poids sec) et hors période de gamétogénèse. Pour chaque température, la relation allométrique entre la réponse physiologique et le poids sec de l'animal (W) a été estimée selon la relation: y=aWb. Un modèle statistique est proposé pour expliquer la filtration ou la consommation d'oxygène (y) en fonction de la température (T) et du poids sec de l'animal (W): y=[a+(b.cT)]. Wd.d est égal à 0,62 pour la filtration et à 0,74 pour la consommation d'oxygène. Les deux fonctions physiologiques augmentent avec l'élévation de la température et atteignent leur maximum à 30°C ( Filtration:...
Tipo: Text Palavras-chave: Filtration; Consommation oxygène; Poids sec; Température; Ostrea edulis.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00048/15886/13308.pdf
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Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf
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Rapport sur l'activité du Laboratoire de La Tremblade - Année 1956 ArchiMer
Trochon, Pierre.
Rapport sur l'activité du Laboratoire ISTPM de La Tremblade concernant l'année 1956
Tipo: Text Palavras-chave: Conchyliculture; Rapport d'activité; Météorologie; Parasite; Mortalité; Reproduction; Claire ostréicole; Crassostrea angulata; Ostrea edulis; Mytilus edulis; Marennes Oléron.
Ano: 1956 URL: http://archimer.ifremer.fr/doc/00069/18068/15612.pdf
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Bonamia ostreae and Ostrea edulis : a stable host-parasite system in France? ArchiMer
Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production...
Tipo: Text Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf
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Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery ArchiMer
Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf
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