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| Verma,Dev Kumar; Rathore,Gaurav. |
| Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different formally... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flavobacterium; Fish; Species diversity; 16S rDNA; Phylogenetic analysis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400969 |
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| Martins,Maira de S.N.; Castro,Alessandra M.M.G. de; Lima,Michele dos S.; Pinto,Vivian da S.C.; Silva,Thaís G. da; Fava,Claudia Del; Depes,Claudio Regis; Okuda,Liria H.; Pituco,Edviges M.. |
| Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovine herpesvirus type 2 (OvHV-2); Histopathology; Qualitative PCR; Quantitative PCR; Phylogenetic analysis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366 |
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| Bashir,Saira; Sarwar,Yasra; Ali,Aamir; Mohsin,Mashkoor; Saeed,Muhammad Azeem; Tariq,Ayesha; Haque,Abdul. |
| The objective of this work was the phylogenetic characterization of local clinical isolates of uropathogenic E. coli with respect to drug resistance. A total of 59 uropathogenic E. coli responsible for community acquired urinary tract infections were included in this study. A triplex PCR was employed to segregate each isolate into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by disc diffusion method. The drugs used were ampicillin, aztreonam, cefixime, cefoperazone, ceftriaxone, cephradine among β-lactam group; amikacin, gentamicin, and streptomycin among aminoglycosides; nalidixic acid and ciprofloxacin from quinolones; trimethoprim-sulfomethoxazole, and tetracycline. Among 59 uropathogenic E. coli isolates majority... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Uropathogenic E. coli; Phylogenetic analysis; Drug resistance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400005 |
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| Fernandes,Maria Judite Bittencourt; Simoni,Isabela Cristina; Harakava,Ricardo; Rivas,Eliana Borges; Arns,Clarice Weis. |
| Infectious bursal disease virus (IBDV) is classified according to the antigenicity and virulence into classical virulent (cv), very virulent (vv), and antigenic variant strains. The molecular basis for the IBDV antigenic variation is well established and is associated to the capsid protein, VP2 (gene VP2 of segment A), whereas both VP2 and the RNA-dependent RNA polymerase, VP1 (gene VP1 of segment B), have been correlated with the virulence. In this study, seventeen Brazilian IBDV samples previously characterized by the VP2 gene as cv (three) and vv (fourteen) strains were genetically and molecularly analyzed for their VP1 gene. All of the strains kept with the same cv or vv classification except one sample, Br/03/DR. This sample was classified as vv by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian infectious bursal disease virus; Very virulent strain; VP1; Sequencing; Phylogenetic analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300024 |
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| Drumond,Betania Paiva; Fagundes,Luiz Gustavo da Silva; Rocha,Raissa Prado; Fumagalli,Marcilio Jorge; Araki,Carlos Shigueru; Colombo,Tatiana Elisa; Nogueira,Mauricio Lacerda; Castilho,Thiago Elias; Silveira,Nelson José Freitas da; Malaquias,Luiz Cosme Cotta; Coelho,Luiz Felipe Leomil. |
| Abstract Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. Primary infection with a single Dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. The four Dengue virus serotypes (1–4) are antigenically and genetically distinct and each serotype is composed of multiple genotypes. In this study we isolated one Dengue virus 1 serotype, named BR/Alfenas/2012, from a patient with dengue hemorrhagic fever in Alfenas, South Minas Gerais, Brazil and molecular identification was performed based... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dengue virus-1; Alfenas; Minas Gerais; Phylogenetic analysis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100251 |
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| Registros recuperados: 46 | |
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