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Estimation of physical and physiological performances of blacklip pearl oyster larvae in view of DEB modeling and recruitment assessment ArchiMer
Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Beliaeff, Benoit; Andrefouet, Serge.
In French Polynesia black pearl farming represents one of the dominant business sectors. However, it still entirely relies on unpredictable Pinctada margaritifera spat collection success, which is itself conditioned by larval development completion. To assess the relationship between larval development and recruitment success, we studied under controlled conditions the effect of food concentration on development, growth, ingestion rate, survival and metabolic rate at the larval stage. Larvae were exposed to four different phytoplankton densities (2,5; 7,5; 15 and 30 cell.μL−1). Larvae survived equally all over the range of phytoplankton concentration with an average survival rate of 16% at the end of experiments. Food concentration significantly affected...
Tipo: Text Palavras-chave: Bivalve larvae; Pinctada margaritifera; Physiology; Energetics; Dynamic energy budget theory; Pearl farming; Aquaculture.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00476/58795/61446.pdf
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Impact of environmental variability on Pinctada margaritifera life-history traits: A full life cycle deb modeling approach ArchiMer
Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Lefebvre, Sebastien; Le Gendre, Romain; Beliaeff, Benoit; Andréfouët, Serge.
The black-lipped pearl oyster (Pinctada margaritifera) is extensively farmed in French Polynesia to produce black pearls. For a sustainable management of marine resources, studying interactions between organisms and environment, and the associated factors and processes that will impact their life cycle and thus modulate population dynamics is a major research priority. Here, we describe black-lipped pearl oyster energy acquisition and use, and its control by temperature and food concentration within the Dynamic Energy Budget (DEB) theory framework. The model parametrization was based on literature data and a specific laboratory experiment. Model validation was carried out thanks to historical in-situ datasets and a dedicated field survey. Three theoretical...
Tipo: Text Palavras-chave: Bivalve; Physiology; Bioenergetics; Dynamic energy budget theory; Environmental change; Pearl farming.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00613/72469/71614.pdf
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Évaluation des potentialités de l'IRM pour la recherche sur la Physiologie des Bivalves Premiers résultats anatomiques et perspectives ArchiMer
Pouvreau, Stephane; Davenel, A; Quellec, S; Rambeau, M.
Nuclear Magnetic Resonance (NMR) is increasingly used in biology and the appearance of imaging and structural and/or metabolic spectroscopy (MRI/MSR) platforms (Rennes, Strasbourg, Marseille...) devoted to this small animal shows the recent keen interest in these research techniques. However, to this day and to our knowledge, no approach of this style has been developed for sea molluscs and even less so for the cup oyster, Crassostrea gigas. Because of its economic importance, this bivalve mollusc is the subject of much research in physiology, to the point of becoming, at IFREMER, a biological model for marine invertebrates. Nonetheless, most of the methodologies developed for studying it are destructive, which (1) prevents individual follow-up, (2)...
Tipo: Text Palavras-chave: Nuclear Magnetic Resonance; Anatomy; Biometry; Physiology; Bivalvia.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1560.pdf
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Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis ArchiMer
Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene.
Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical...
Tipo: Text Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf
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Evolution des acides nucleiques au cours du developpement larvaire de la crevette rose Palaemon serratus (Pennant). ArchiMer
Campillo, Albert; Regnault, M.; Luquet, P..
The evolution of nucleic acids in larvae of P. serratus reared in the laboratory from hatching to metamorphosis has been studied. From the 1st larval stage to the post-larval stage, DNA and RNA concn decrease: from 185 to 95 uMoles-bases/g PSD for DNA and from 133.5 to 78.75 uMoles-bases for RNA. The concn decrease is not regular; it is emphasized between stages 4 and 5 on the one hand, and between stage 7 and the post-larva on the other hand. The amount of DNA and RNA per larva increases proportionally to the wet weight from stage 1 to the post-larva. As the weight is multiplied by 7.6, total DNA and total RNA are multiplied by 3.80 and 4.25 respectively. The RNA/DNA ratio has a mean value of 0.77; it increases slightly from stage 1 (0.72) to the...
Tipo: Text Palavras-chave: Calliphora; Crangon crangon; Palaemon serratus; Nucleic acids; Larval development; Invertebrate larvae; Biochemistry; Physiology.
Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/publication-2023.pdf
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Activité respiratoire en continu de l'huître Crassostrea gigas : mise au point d'une nouvelle méthodologie ArchiMer
Bougrier, Serge; Geairon, Philippe.
A continuous monitoring system to measure the respiration of Crassostrea gigas has been developped. The metabolic function estimated by oxygen consumption has been monitored during periods of 24 h, without food, at a temperature constant of 15°C. Preliminary results showed that the mean percentage of oyster activities was 50 % with a high variability from 0 to 100 %. The number of activity periods per day (24 h) is function of the oysters with variation between 0 and 7.
Tipo: Text Palavras-chave: Physiology; Respiration; Crassostrea gigas; Oysters; Physiologie; Respiration; Crassostrea gigas; Huîtres.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3046.pdf
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Bilan énergétique chez les mollusques bivalves : terminologie et méthodologie - Compte rendu du groupe de travail réuni au laboratoire national "Ecosystème conchylicole" de l'IFREMER La Tremblade (13 et 14 septembre 1984) ArchiMer
Heral, Maurice; Lucas, Albert; Deslous Paoli, Jean-marc; Goulletquer, Philippe; Masse, Henri; LABEIM (Laboratoire de Biologie et d'Ecologie des Invertébrés Marins).
En septembre 1984, MM. HERAL et DESLOUS-PAOLI organisèrent à La Tremblade, une "réunion méthodologie expérimentale mollusques". Cette rencontre faisait suite à un bref état qui s'était engagé au Congrès de Montpellier sur les "Bases biologiques de l'Aquaculture", où il était apparu que la terminologie et la méthodologie concernant le métabolisme des bivalves devaient être clarifiées. Grâce à une large information auprès des spécialistes français de la physiologie des bivalves, la réunion à La Tremblade a réuni un grand nombre de participants très motivés. Pendant 2 jours des discussions très animées ont permis de bien comprendre les échanges entre les bivalves et leur environnement et d'y jeter un regard neuf, ce qui impliquait une analyse critique du...
Tipo: Text Palavras-chave: Terminologie; Méthodologie; Bilan d'énergie; Physiologie; Bivalve; Terminology; Methodology; Energy budget; Physiology; Bivalve molluscs.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00014/12562/9432.pdf
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Mise en place d'un plan de sélection génétique pour l'ormeau européen Haliotis tuberculata ArchiMer
Lachambre, Sébastien.
In the context of an increasing demand and a declining supply from fisheries, worldwide aquaculture production of abalone has greatly increased in the last decades. Selective breeding has been initiated to improve growth in major abalone species. In France, the abalone production was initiated 30 years ago and struggles to develop. This CIFRE PhD co-financed by France Haliotis, the only French hatchery in activity, was initiated to study early domestication process of farmed abalone population and to provide knowledge required to design a selective breeding program adapted to this company. Wild and farmed populations were compared, showing that, until 2013, farmed and wild abalone presented similar performances. However, lower physiological and behavioural...
Tipo: Text Palavras-chave: Sélection génétique; Ormeaux; Croissance; Physiologie; Comportement; Selective Breeding; Abalone; Growth; Physiology; Behaviour.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00441/55231/56711.pdf
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Environnement et jeunes stades de vie chez le poisson : la plasticité développementale comme réponse aux contraintes hypoxiques et thermiques ArchiMer
Cadiz Barrera, Laura.
In the context of global change, decrease in oxygen availability (hypoxia) combined with rising water temperature are especially prevalent in coastal regions, towards which marine fish larvae may drift at the end of their development. It is well admitted that the physiological regulations implemented by organisms to cope with their environment during the early life stages of life can cause profound consequences in their subsequent life-history trajectory (developmental plasticity). Therefore, the main objective of this thesis was to investigate whether ecologically relevant conditions of oxygenation (40% and 100% air saturation) combined with thermic conditions (15 and 20 °C), occurring at the last stages of larval development of European sea bass...
Tipo: Text Palavras-chave: Hypoxie; Réchauffement des océans; Physiologie; Bar Européen; Plasticité développementale; Hypoxia; Ocean warming; Physiology; European sea bass; Developmental plasticity.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00498/60973/64384.pdf
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Rapport de mission aux Etats-Unis du 4 au 22 janvier 1988 ArchiMer
Fauvel, Christian.
During this visit, research work on the physiology and zootechny of reproduction of different US teams were compared to ours. It is clear that we have the same problems to solve and that research programs are rather similar, but they must take into account the local conditions. This fact limits the possibilities of cooperation. Nevertheless a very good knowledge of each work has been noted.
Tipo: Text Palavras-chave: Reproduction; Physiologie; Crustacés; Maturation; Zootechnie; Reproduction; Maturation; Physiology; Zootechny.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00374/48516/48789.pdf
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Constraints and Priorities for Conducting Experimental Exposures of Marine Organisms to Microplastics ArchiMer
Paul-pont, Ika; Tallec, Kevin; Gonzalez-fernandez, Carmen; Lambert, Christophe; Vincent, Dorothee; Mazurais, David; Zambonino-infante, Jose-luis; Brotons, Guillaume; Lagarde, Fabienne; Fabioux, Caroline; Soudant, Philippe; Huvet, Arnaud.
Marine plastic pollution is a major environmental issue. Given their ubiquitous nature and small dimensions, ingestion of microplastic (MP) and nanoplastic (NP) particles and their subsequent impact on marine life are a growing concern worldwide. Transfers along the trophic chain, including possible translocation, for which the hazards are less understood, are also a major preoccupation. Effects of MP ingestion have been studied on animals through laboratory exposure, showing impacts on feeding activity, reserve depletion and inflammatory responses, with consequences for fitness, notably reproduction. However, most experimental studies have used doses of manufactured virgin microspheres that may not be environmentally realistic. As for most...
Tipo: Text Palavras-chave: Microplastics; Nanoplastics; Experimental exposure; Impacts; Marine organisms; Physiology.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56088/57621.pdf
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Response of Mytilus edulis to enhanced phytoplankton availability by controlled upwelling in an oligotrophic fjord ArchiMer
Strohmeier, Tore; Strand, Oivind; Alunno-bruscia, Marianne; Duinker, Arne; Rosland, Rune; Aure, Jan; Erga, Svein R.; Naustvoll, Lars J.; Jansen, Henrice M.; Cranford, Peter J..
The controlled upwelling of nutrient-rich deep water in oligotrophic coastal regions has been proposed as a means of increasing phytoplankton and, subsequently, bivalve aquaculture production. This was tested as part of a large-scale upwelling experiment in an oligotrophic environment (Lysefjord, Norway). The mean chlorophyll a concentration in the upwelling area was consistently higher than at the control site (mean ± SD: 3.3 ± 1.9 and 1.5 ± 0.6 mg Chl a m-3, respectively) during the 4 mo of controlled upwelling. After 2 mo with upwelling, the dry flesh weight of 1 yr-old and 2 yr-old mussels was 95% and 24% higher, respectively, than that of the mussels at the control site. The 1 yr-old mussels at the upwelling site achieved up to 2.4-fold higher dry...
Tipo: Text Palavras-chave: Bivalve aquaculture; Production carrying capacity; Physiology; Fjord ecosystems; Shell growth; Tissue growth.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00241/35200/33704.pdf
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Shore-level size gradients and thermal refuge use in the predatory sea star Pisaster ochraceus: the role of environmental stressors ArchiMer
Monaco, Cristian; Wethey, David S.; Gulledge, Shadow; Helmuth, Brian.
The body size of a mobile intertidal invertebrate can determine its access to different microhabitats, and thus alter its exposure to environmental stressors. We surveyed a rocky intertidal keystone predator, the sea star Pisaster ochraceus, and characterized size-dependent distribution, defined by individuals' shore level and refuge use. At 2 field sites (in California and Oregon, USA) we examined temporal and geographical variability in habitat selection. We evaluated the hypothesis that environmental drivers measured on-site and body temperatures measured using biomimetic sensors (i.e. 'robo-sea stars'), explained the observed distribution patterns, including shore-level size gradients, with larger animals lower on the shore. We tested the effect of...
Tipo: Text Palavras-chave: Ecology; Physiology; Rocky intertidal; Environmental stress; Climate change; Temperature; Desiccation; Solar radiation; Body size; Behavior.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00605/71695/70137.pdf
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Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. ArchiMer
Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert.
The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf
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Evolution de la biomasse et de la physiologie du zooplancton sur la côte nord de Bretagne en relation avec l'échouage de l'Amoco Cadiz : résultats préliminaires pour la période du 16 mars au 20 mai 1978 ArchiMer
Samain, Jean-francois; Le Fevre, J.; Moal, J.; Daniel, J.y.; Boucher, Jean.
First results about distribution and changes of biomass (dry weight and total soluble proteins), physiology (amylase, trypsin) and faunistic composition of zooplankton are reported for the two months period following the spill of AMOCO CADIZ on the Britany Northern coast. A shortage of biomass in the aber area is attributed to hydrocarbons. The low level of the mean value of biomasse on the North coasts, and the pecularities of estuarine station of the Lannion bay and of Trieux area are reported and discussed.
Tipo: Text Palavras-chave: Amoco Cadiz; Zooplancton; Biomasse; Physiologie; Marée noire; Amoco Cadiz; Zooplankton; Biomass; Physiology; Spill.
Ano: 1978 URL: http://archimer.ifremer.fr/doc/00306/41728/40939.pdf
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Bases génétiques des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas (Thunberg) ArchiMer
Collet, Bertrand.
Growth is a very important trait in the oyster Crassoslrea gigas both from an economical and scientific point of view. Many questions remain unanswerable or are still discussed . From a hatchery-based population, the constancy in time of growth performances From larval to adult stage was studied. The influence of physiological parameters (energy acquisit ion and expenditure) and parentage on growth was estimated. ln the literature, the relationship between larval growth and post-Iarval growth is debated . Our study shows that the larval growth is positively correlated with the post-Iarval growth. This result validates the hatchery practices consisting in culling the small larvae. The growth performances are stable over the time and show no compensation....
Tipo: Text Palavras-chave: Crassostrea gigas; Croissance; Physiologie; Analyse de parenté; Microsatellite DNA; Crassostrea gigas; Growth; Physiology; Parentage analysis; Microsatellite DNA.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00108/21939/19532.pdf
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Use of diatom motility features as endpoints of metolachlor toxicity ArchiMer
Coquille, Nathalie; Jan, Gwilherm; Moreira, Aurelie; Morin, Soizic.
Many recent ecotoxicological studies suggest a relationship between freshwater contamination and increasing abundances of motile diatoms (potentially able to move). The capacity to escape would present advantages to species in polluted environments. However, actual motility as a response to toxicants had not been described and required experimental validation. We designed a specific experiment to assess how a field-isolated diatom (Gomphonema gracile) distributes energy to in situ resistance (increased population growth or photosynthesis) and escape (behavioral changes), when exposed to increasing concentrations of the herbicide metolachlor. We report here the dose-time dependent responses of G. gracile populations. They coped with low contamination by...
Tipo: Text Palavras-chave: Diatoms; Physiology; Behavior; Pesticide exposure; Motility; CASA.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36521/35081.pdf
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Dynamique des populations bactériennes au cours de deux productions expérimentales de phytoplancton marin naturel ArchiMer
Bianchi, M.a.; Martin, Y.p..
Quantitative and qualitative variations of the hetero­trophic bacterial population, during two experimental induced phytoplanktonic blooms are described. When the phytoplanktonic population deceases, the bacte­rial population exhibits a significant increase. The spring microflore is quite different from the fall ones. Microbiological, physiological and biochemical fluctuations indicate qualitative fluctuations. Bacterial strains are identified as "bacterial profiles".
Tipo: Text Palavras-chave: Plancton; Bactéries; Dynamique; Physiologie; Structure; Plankton; Bacteria; Dynamic; Physiology; Structure.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00305/41669/40878.pdf
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Early weaning of marine fish larvae onto microdiets: Constraints and perspectives ArchiMer
Person, L.
Weaning small sized marine fish larvae at first feeding directly onto compound pellets is still difficult, while good results can be obtained when used in combination with live prey or when a prefeeding period on live prey is provided up to a size of about 2-3 mg. Microdiets are generally well ingested as their acceptability and visual detection may be improved using feeding activators and increasing good perception contrast. In theory, food may be processed to have a correct nutritional balance, selection of high digestible components is very important as larval digestive system is not fully functional during the first 2 or 3 weeks of life.
Tipo: Text Palavras-chave: Hatching; Fatty acids; Amino acids; Nutritional requirements; Feeding behaviour; Stomach; Digestive system; Enzymes; Digestion; Physiology; Diets; Pellet feeds; Feeding; Fish larvae.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1422.pdf
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Physiological responses during acute stress recovery depend on stress coping style in European sea bass, Dicentrarchus labrax. ArchiMer
Ferrari, Sebastien; Rey, Sonia; Høglund, Erik; Øverli, Øyvind; Chatain, Beatrice; Mackenzie, Simon; Bégout, Marie-laure.
Individual stress coping style (reactive, intermediate and proactive) was determined in 3 groups of 120 pit tagged European seabass using the hypoxia avoidance test. The same three groups (no change in social composition) were then reared according to the standards recommended for this species. Then, 127 days later, individuals initially characterized as reactive, intermediate or proactive were submitted to an acute confinement stress for 30 min. Blood samples were taken to measure plasma cortisol levels 30 min (Stress30) or 150 min (Stress150) after the end of the confinement stress. Individuals were then sacrificed to sample the telencephalon in order to measure the main monoamines and their catabolites (at Stress30 only). Individuals from Stress150 were...
Tipo: Text Palavras-chave: Fish; Behavior; Physiology; Personality; Brain; Transcriptomic.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71330/69760.pdf
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