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| Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B. |
| Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida... |
| Tipo: Text |
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf |
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| Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle. |
| Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus.... |
| Tipo: Text |
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf |
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| Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene. |
| Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to... |
| Tipo: Text |
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf |
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| Mersni Achour, Rachida; Imbert-auvray, Nathalie; Huet, Valerie; Ben Cheick, Yosra; Faury, Nicole; Doghri, Ibtissem; Rouatbi, Sonia; Bordenave, Stéphanie; Travers, Marie-agnes; Saulnier, Denis; Fruitier-arnaudin, Ingrid. |
| Extracellular products (ECPs) of the French V. tubiashii strain 07/118 T2 were previously reported to be toxic for the Pacific oyster Crassostrea gigas. In this study we now assessed host cellular immune responses and bacterial potential effectors by which these ECPs can be associated with host damages. The adhesion capacity (28% inhibition) and phagocytosis ability (56% inhibition) of oyster hemocytes were the main functions affected following in vitro contact between hemocytes and V. tubiashii ECPs. This may be linked to the demonstration of the capability of ECPs to cleave various cellular substrates as oyster collagen. Moreover, a strong metalloproteolytic activity was recorded with general (azocasein) and specific (ADAM) substrates and characterized... |
| Tipo: Text |
Palavras-chave: ECPs; Oyster immune responses; Thermolysin-metalloprotease; Mass spectroscopy; ADAM substrate; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00211/32203/30642.pdf |
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| Silva,Emanuell Felipe Beserra da; Fróes,Charles Nunes; Souza,Diego Moreira de; Soares,Roberta; Peixoto,Silvio; Wasielesky,Wilson; Ballester,Eduardo Luis Cupertino. |
| O objetivo deste trabalho foi avaliar o uso de probióticos na produção de pós-larvas do camarão-rosa (Farfantepenaeus brasiliensis). Utilizou-se o delineamento experimental inteiramente casualizado, com dois tratamentos - uso de probióticos (Bacillus spp.) e controle com uso de antibiótico (eritromicina) - e quatro repetições. Pós-larvas no estágio PL1 foram estocadas em cada parcela experimental (3,0 L) e cultivadas até o estágio PL10. As variáveis de qualidade de água e o desenvolvimento de bactérias do gênero Vibrio, na água de cultivo, foram monitorados ao longo do experimento. Ao final do período experimental, as pós-larvas foram avaliadas qualitativamente e quanto à sobrevivência, ao peso e ao comprimento. Nas variáveis de qualidade de água, apenas o... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus; Farfantepenaeus brasiliensis; Vibrio; Antibiótico; Crescimento; Sobrevivência. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600019 |
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| Vieira,Genyess Ribeiro Arthur da Silva; Soares,Mariana; Ramírez,Norha Constanza Bolívar; Schleder,Delano Dias; Silva,Bruno Corrêa da; Mouriño,José Luiz Pedreira; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento. |
| Abstract The objective of this work was to evaluate the effect of Lactobacillus plantarum on the preservation of fresh mussels and its effect on the attractiveness, consumption, and midgut bacterial microbiota of Pacific white shrimp broodstock. The experiment evaluated mussels stored with L. plantarum at 4ºC. The controls were: mussels stored at -18ºC without L. plantarum; mussels stored at -18ºC with L. plantarum; and mussels stored at 4ºC without L. plantarum. Microbiological analyses on mussels were performed on days 1, 7, 15, 30, 45, and 60 after processing. Additionally, mussels preserved with L. plantarum and stored at 4ºC, and mussels stored at -18ºC without L. plantarum were evaluated after 15 days for attractiveness, consumption, and midgut... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus plantarum; Litopenaeus vannamei; Vibrio; Probiotic. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101799 |
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| Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC. |
| This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%... |
| Tipo: Resistencia a infecção |
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471 |
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