|
|
|
| Registros recuperados: 77 | |
|
| |
|
| |
|
|
| Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC. |
| This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%... |
| Tipo: Resistencia a infecção |
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471 |
| |
|
|
| Rossato,J.M.; Brito,B.G.; Kobayashi,R.K.T.; Koga,V.L.; Sarmiento,J.J.P.; Nakazato,G.; Lopes,L.F.D.; Balsan,L.A.G.; Grassotti,T.T.; Brito,K.C.T.. |
| ABSTRACT Diarrheagenic (DEC) and avian pathogenic Escherichia coli (APEC) are associated with intestinal and extra-intestinal infections (ExPEC), respectively. We aimed to analyze the antimicrobial susceptibility, gene encoding virulence factors associated to DEC and APEC, and phylogenetic classification in E. coli isolated from 320 samples of feed and ingredients. Antimicrobial susceptibility was performed using the disk diffusion method and Multiple Antibiotic Resistance (MAR) Index and Multi-Drug Resistance (MDR) were calculated. Phylogenetic classification was performed on samples harboring DEC and/or APEC virulence-associated genes. A total of 110 E. coli strains were isolated in 15% (49/320) of the evaluated inputs (n=13 vegetable meal; n=33 animal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poultry production; Animal feed; Antimicrobials; Virulence; Resistance. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601968 |
| |
|
| |
|
|
| Carneiro,V.C.; Lessa,D.A.B.; Guttmann,P.M.; Magalhaes,H.; Aquino,M.H.C.; Cunha,L.E.R.; Arais,L.R.; Cerqueira,A.M.F.. |
| ABSTRACT Respiratory diseases are common in young horses but little is known about such infections in mule foals. This study aimed to characterize Escherichia coli and Klebsiella sp. isolated from tracheal wash (TW) and fecal samples (FS) of mule foals, with or without cytological evidence of respiratory disease. Strains were analyzed against 13 antimicrobials, for presence of Extended spectrum beta-lactamase (ESBL), and virulence genes. Phylogrouping and Randomic (RAPD)-PCR profiles were used to evaluate their genetic relatedness. E. coli strains from TW and FS showed greatest resistance to tetracycline, while Klebsiella strains were mainly resistant to ampicillin; multidrug resistance and ESBL production were also detected. The blaCTX gene prevailed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Klebsiella; Respiratory disease; Virulence; Antimicrobial resistance. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501073 |
| |
|
|
| Lima,D.A.; Furian,T.Q.; Pillati,R.M.; Silva,G.L.; Morgam,R.B.; Borges,K.A.; Fortes,F.B.B.; Moraes,H.L.S.; Brito,B.G.; Brito,K.C.T.; Salle,C.T.P.. |
| Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x108 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Salmonelosis; Virulence. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200257 |
| |
|
|
| Silva,Gisele Barata da; Araújo,Leila Garcês de; Lobo,Valácia Lemes da Silva; Prabhu,Anne Sitarama; Rêgo,Marcela Cristiane Ferreira; Paes,Eduardo Tavares; Filippi,Marta Cristina Corsi de. |
| Eight additional rice cultivars as differentials were selected to identify pathotypes occurring in the Tocantins State. They were BRS Jaburu, BRS Taim, BRS Biguá, BR IRGA-417, Epagri 109, Javaé, Metica-1 and Supremo. These differentials were used in addition to the international set to determine the pathogenic diversity of 193 Pyricularia oryzae isolates collected during 1994-2002 from irrigated rice cultivars. They were conserved in Pyricularia culture collection of Embrapa Rice and Bean Research center. From 193 P. oryzae isolates 38 pathotypes were identified based on leaf blast reactions of international set and 29 pathotypes based on these additional differentials. The predominant pathotypes (TI-1, TG-2, TD-15 and TF-2) were represented by 53% of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pyricularia grisea; Rice leaf blast; Physiologic races; Oryza sativa; Virulence; Resistance; Differential varieties. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400019 |
| |
|
|
| Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de. |
| The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003 |
| |
|
|
| Almeida,Rosana Macedo de; Barbosa,André Victor; Lisbôa,Rodrigo de Castro; Santos,André Felipe das Mercês; Hofer,Ernesto; Vallim,Deyse Christina; Hofer,Cristina Barroso. |
| ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Listeriosis; Virulence; Food; PFGE; Brazil. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282 |
| |
|
|
| Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming. |
| ABSTRACT Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates. Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922. Results:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015 |
| |
|
|
| Rusak,Leonardo Alves; Junqueira,Ricardo Magrani; Hofer,Ernesto; Vallim,Deyse Christina; Asensi,Marise Dutra. |
| Abstract Yersinia enterocolitica is a widespread Gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. Potentially pathogenic Y. enterocolitica has been isolated in Brazil, from human, environmental, food, and animal sources. Herein we report a genome sequence of Y. enterocolitica subsp. palearctica strain YE 19, serotype O:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in Rio de Janeiro in 2005. The results corroborate other findings that this strain harbors a set of virulence determinants that could play a role in host pathoadaptation and may also justify the successful dissemination of bioserotype 4/O:3 in Brazil. The presence of strains harboring all of these... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Yersinia enterocolitica; Virulence; Brazil; Next-generation sequencing. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500550 |
| |
|
|
| Nweze,EI; Okafor,JI. |
| Scedosporium species are an emerging opportunist group of fungi that have been found to cause infections in both immunocompetent and non-immunocompetent individuals. The infections are not regularly distributed among different countries of the world either because of improper identification or other geographical reasons. Strange as it may, disseminated systemic infections have only been reported in some specific countries. We used a mouse model of disseminated infection to assess if strains from Nigeria were virulent and compared it to a few other strains from other countries. S. apiospermum isolated from Nigeria were clearly less virulent than those obtained elsewhere. This may be the reason why this group of fungi has not been associated with specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scedosporium; Inocula; Virulence; Fungi. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300012 |
| |
|
| |
|
| |
|
|
| Liberatore,Ana Maria Alvim; Tomita,Sandra Kimie; Vieira,Mônica Aparecida Midolli; Toti Jr.,Cyro; Heckmaier,João; Gomes,Tânia Aparecida Tardelli. |
| The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings) was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2%) adhered to HeLa cells, 21 (77.8%) of which presented the aggregative adherence pattern (AA) that characterize the Enteroaggregative E. coli pathotype (EAEC). Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Escherichia coli; Aggregative adherence; Virulence. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100003 |
| |
|
|
| Alves,Lucas Bocchini Rodrigues; Freitas Neto,Oliveiro Caetano de; Batista,Diego Felipe Alves; Barbosa,Fernanda de Oliveira; Rubio,Marcela da Silva; Souza,Andrei Itajahy Secundo de; Almeida,Adriana Maria de; Barrow,Paul Andrew; Berchieri Junior,Angelo. |
| Abstract Salmonella Gallinarum is a host-restrict pathogen that causes fowl typhoid, a severe systemic disease that is one of the major concerns to the poultry industry worldwide. When infecting the bird, SG makes use of evasion mechanisms to survive and to replicate within macrophages. In this context, phoPQ genes encode a two-component regulatory system (PhoPQ) that regulates virulence genes responsible for adaptation of Salmonella spp. to antimicrobial factors such as low pH, antimicrobial peptides and deprivation of bivalent cations. The role of the mentioned genes to SG remains to be investigated. In the present study a phoPQ-depleted SG strain (SG ΔphoPQ) was constructed and its virulence assessed in twenty-day-old laying hens susceptible to fowl... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mutation; Virulence; Fowl typhoid; Adaptation; Chickens. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300601 |
| |
|
|
| Chaves,Guilherme M.; Cavalcanti,Maria Auxiliadora de Q.; Carneiro-Leão,Ana Maria dos A.; Lopes,Silvia L.. |
| With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL-1) activity. The other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase (Pz=0.482) and proteinase (0.780 U.mL-1) activity. The strains were inoculated via intraperitoneum in immunosuppressed and non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and kidneys were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Extracellular enzymes; Virulence; Experimental infection. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300010 |
| |
|
|
| Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco. |
| Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005 |
| |
|
| |
| Registros recuperados: 77 | |
|
|
|
