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Registros recuperados: 49 | |
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Alavarez,JM; Ferreira,CSA; Ferreira,AJP. |
In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleic-acid... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300001 |
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Carvalho,CMC; Litz,FH; Fernandes,EA; Silveira,MM; Martins,JM da S; Fonseca,LA; Zanardo,JA. |
The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal); B. Grain sorghum (Whole Sorghum + soybean meal). At 35 and 42 days were evaluated mineral matter (A %), calcium (Ca%), phosphorus (P%), pH and dry matter (DM %) of the poultry litter. To evaluate the footpad dermatitis were evaluated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis; Calcium; Environmental impact; Phosphorus. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300010 |
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Venturini,Marcela Tonini; Araújo,Taylane da Silva; Abreu,Emanuel Felipe Medeiros; Andrade,Eduardo Chumbinho de; Santos,Vanderlei da Silva; Silva,Marcos Roberto da; Oliveira,Eder Jorge de. |
ABSTRACT Despite the widespread distribution of the Cassava common mosaic virus (CsCMV) in Brazil, little is known about the losses it causes in yield. The effect of CsCMV on different varieties was evaluated by reference to several agronomic traits. Four field trials were established in 2012/2013 and 2013/2014 using six varieties of cassava. Following mechanical inoculation with CsCMV, the presence of the virus was confirmed using the ELISA assay. The evaluated traits were plant height (PH), dry matter content (DMC), harvest index (HI), aerial part yield (APY), root yield (RoY), and starch yield (StY) in both inoculated and non-inoculated plants. Overall, the presence of the virus contributed little to the reduction in PH, HI, and DMC across the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta Crantz; Viruses; Root yield; Disease. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000600520 |
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Juhász,Ana Cristina Pinto; Silva,Derly José Henriques da; Zerbini Júnior,Francisco Murilo; Soares,Bruno Oliveira; Aguilera,George Alberto Hial. |
The tomato is a crop of great economical importance, however it is susceptible to a large number of pests and diseases, including viral disease for which the best control strategy is genetic resistance. The disease, caused by Pepper yellow mosaic virus (PepYMV) has become a recent problem. Consequently, the idea of this work was to screen 376 accessions of Lycopersicon sp. to find possible sources of resistance to PepYMV. Out of 355 accessions of L. esculentum inoculated with PepYMV, 52 did not express symptoms. However, the virus reached high concentration in the tissues as measured by indirect ELISA, and therefore they were not considered as safe sources of resistance. Among 21 accessions of wild Lycopersicon species, one of L. hirsutum was shown to be... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon hirsutum; Genetic resources; Viruses; Potyvirus. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500014 |
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Seibert,Caroline H.; Pinto,Aguinaldo R.. |
Shrimp aquaculture has been dramatically affected by many pathogenic diseases, mainly caused by five viruses: IHHNV, YHV, TSV, WSSV, and IMNV. Here we provide a state-of-the-art overview of these shrimp viruses, with emphasis on distribution, pathology, morphology, and genomic organization, in addition to current diagnostic methods and intervention practices. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shrimp aquaculture; Viruses; Diagnostic methods; Disease control. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300002 |
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Marinho,Rebeca C.; Martins,Gabrielle R.; Souza,Kelma C.; Sousa,Ana Lídia M.; Silva,Sabrina Tainah C.; Nobre,Juliana A.; Teixeira,Maria F.S.. |
Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Standardization; Multiplex; Viruses; CAEV; MVV. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500083 |
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Registros recuperados: 49 | |
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