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| Registros recuperados: 2.284 | |
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| Marco Antonio M. A. Russo; Sonja Veljovic-Jovanovic; Adalgisa Belligno. |
| The aim of this work was to study, as a function of the different availability of nitrogen in the reduced form, mineral and organic, the induction of the synthesis of some ROS-scavenging molecules and the evolution of some enzymatic activities such as ascorbate peroxidase (APX) and polyphenoloxidase (PPO). 
Chicory seedlings were grown in nutritive solution for 35 days in controlled conditions. On the 14th day, one third of the plants was transferred into a nutritive solution containing (NH4)2SO4 60 mM, one third was transferred into a medium containing Urea 60 mM, and the remaining was let grow into the nutrition solution, as a control. Three samplings of leaves were performed, respectively after 21, 28 and 35 days of... |
| Tipo: Manuscript |
Palavras-chave: Plant Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4173/version/1 |
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| Elibio L. Rech. |
| The Amazon basin is an area of mega-biodiversity. Different models have been proposed^1-8^ for the establishment of an effective conservation policy, increasing sustainability and adding value for biodiversity. Currently, a broad spectrum of technologies from genomics to synthetic biology is available, and these permit the collection, manipulation and effective evaluation of countless organisms, metabolic pathways and molecules that exist as potential products of a large, biodiverse ecosystem. The use of Genomics and synthetic biology may constitute an important tool and be a viable option for the prospection, evaluation and manipulation of biodiversity as advocated as well as be useful for developing methods for sustainable use and the production of novel... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Genetics & Genomics; Earth & Environment. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/5759/version/1 |
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| Gregory Fairn; Sergio Grinstein. |
| We used genetically-encoded fluorescent probes to visualize the distribution of phosphatidylserine (PS) in live S. cerevisiae. The majority of the PS was found to reside in the cytosolic leaflet of the plasma membrane. Remarkably, PS was polarized, accumulating in bud necks, the bud cortex and the tips of mating projections. Polarization required vectorial delivery of PS-enriched secretory and recycling vesicles. Rapid dissipation of the PS gradient is prevented by the slow diffusion of lipids along the plasmalemmal inner leaflet, estimated by photobleaching recovery measurements to be over an order of magnitude slower than in mammalian cells. In mutants lacking PS-synthase the absence of PS was associated with, and likely responsible for impaired... |
| Tipo: Manuscript |
Palavras-chave: Microbiology; Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4232/version/1 |
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| Lee M. Talbot. |
| Background/Question/Methods: Many ecologists are skilled at identifying environmental problems and defining solutions, but not at achieving concrete action to implement those insights. The question, then, is how to help ecologists translate environmental science into policy and action. Most ecologists' education and training do not prepare them to be effective in the policy arena, much less in ways to achieve action. Ecologists find disincentives in the form of the academic attitude that somehow the application of science is inferior to so-called "pure science." This ivory tower holdover has been largely discredited in the context of environmental issues, and the discipline of conservation biology exists explicitly to foster... |
| Tipo: Manuscript |
Palavras-chave: Earth & Environment. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3606/version/1 |
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| Etienne Joly. |
| To obtain an optimised form of BFP for use as a reporter gene in mammalian cells, the brightest available GFP form, EGFP, was mutated at 5 different positions, yielding 8 different mutagenised forms of BFP. The intensity of the fluorescent signals attained in mammalian cells with all these various versions of BFP was analysed by flow cytometry of transiently transfected COS 7 cells. The best mutant obtained can be detected readily both by flow cytometry and fluorescence microscopy, even when expressed together with GFP. To explore whether cellular localization could enhance the fluorescence signals any further, plasmid constructs were made to target optimised versions of GFP and BFP to the nucleus, the endoplasmic reticulum (ER) and the cell surface.... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1259/version/1 |
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| Andy Maloney; Lawrence J. Herskowitz; Steven J. Koch. |
| We show here the effects of heavy-hydrogen water (^2^H~2~O) and heavy-oxygen water (H~2~^18^O) on the gliding speed of microtubules on kinesin-1 coated surfaces. Increased fractions of isotopic waters used in the motility solution decreased the gliding speed of microtubules by a maximum of 21% for heavy-hydrogen and 5% for heavy-oxygen water. We discuss possible interpretations of these results and the importance for future studies of water effects on kinesin and microtubules. We also discuss the implication for biomolecular devices incorporating molecular motors. |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/7004/version/1 |
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| Jorge M. Dinis; David E. Barton; Jamsheed Ghadiri; Deepa Surendar; Kavitha Reddy; Fernando Velasquez; Carol M. Chaffee; Wendy Lee; Helen Gavrilova; Hazel Ozuna; Samuel A. Smits; Cleber C. Ouverney. |
| The Bacteria Domain experienced an explosion of novel lineages identified within the last decade, especially of lineages made entirely of uncultured members. Since numerous cultivable bacteria have been shown to be instrumental in human development, health, and diseases, it is reasonable to speculate that strains from uncultured groups, which comprise nearly 80% of the human gut and 68% of human oral microbial consortia, participate in similar functions. The study of human-associated uncultured bacteria, however, has many practical limitations, such as access to patient samples, unpredictable microbial composition, and low relative abundance, all of which challenge experimental promptness and reproducibility. We propose that uncultured bacteria from... |
| Tipo: Manuscript |
Palavras-chave: Microbiology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/5757/version/1 |
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| Toni P. Miles; Stephen Hanson; LaCreis Kidd; Avonne Connor; Saeed A. Jortani. |
| Within the realm of medical care, Self-Identified Race and Ethnicity (SIRE) categories are promoted as an inexpensive tool to identify underlying genotypic diversity. Scientific opinion is divided about the adequacy of SIRE to serve this function. If genetic diversity can guide medical decisions, it is important to know the effectiveness of genetic screening via SIRE. Proper development of self-reported measures such as SIRE requires sensitivity and specificity studies. These types of formal evaluation are largely absent for SIRE. To begin this formal process, we estimate the sensitivity of SIRE in screening for variant Single Nucleotide Polymorphism (SNP). Our results indicate that the current use of SIRE is inadequate to screen for selected... |
| Tipo: Manuscript |
Palavras-chave: Genetics & Genomics. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1524/version/1 |
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| Stephan Meinke; Carolin Böhm; Sibel Durlanik; Carsten Watzl. |
| Activation of natural killer cells can be mediated by different receptors. Stimulation of the receptors 2B4, NTB-A and CRACC, members of the SLAM-related receptor family, induces cytotoxicity and cytokine production. The surface expression of 2B4 and other activating natural killer cell receptors is down-modulated after receptor engagement, which results in a weaker response to consecutive stimulation. We tested whether this regulatory mechanism applies to all SLAM-related receptors expressed by primary human natural killer cells. After co-culture with target cells expressing the respective ligands different effects on receptor surface expression were observed. While 2B4 ex-pression was strongly reduced, NTB-A showed less prominent down-modulation and the... |
| Tipo: Manuscript |
Palavras-chave: Immunology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4917/version/1 |
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| Romulus Breban; Virginie Supervie; Justin Okano; Raffaele Vardavas; Sally Blower. |
| The Centers for Disease Control (CDC) is currently attempting to eliminate syphilis in the United States (US); to ensure that their control strategies will be effective it is important to understand the transmission dynamics of syphilis. Epidemics of certain infectious diseases (e.g., influenza) can rise and fall with a well-defined periodicity; this cycling behavior is important because it can have significant implications for the design and effectiveness of control strategies. Here we discuss the methodology that has been used to identify epidemic cycles in longitudinal data sets, and the endogenous and exogenous mechanisms that generate cycling. We then examine the recently proposed hypothesis that syphilis epidemics cycle. This hypothesis was proposed... |
| Tipo: Manuscript |
Palavras-chave: Ecology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1373/version/1 |
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| Daniel Casasanto. |
| If understanding action words involves mentally simulating our own actions, then the neurocognitive representation of word meanings must differ for people with different kinds of bodies, who perform actions in systematically different ways. In a test of the _Body-Specificity Hypothesis_, right- and left-handers were compared on two motor-meaning congruity tasks. Double dissociations in both action execution and recognition memory results showed that right and left handers form body-specific representations of words for manual actions. |
| Tipo: Manuscript |
Palavras-chave: Neuroscience. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1321/version/1 |
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| Tiefeng You; I. C. Baianu. |
| The soybean-derived products are among the most important agricultural products in the USA and the world. Conventional analytical methods for soybean composition analysis are both time consuming and costly. Faster and less expensive methods are required for most practical applications. To improve the accuracy, reliability and sensitivity of NIR, major advancements in instrumentation, as well as, data analysis / calibration methodology are required. Novel NIR instruments, such as DA-NIR and FT-NIR spectrometers developed in recent years have the potential for improving significantly the quantification of soybean composition at a reasonable cost. We present representative calibrations and data for intact soybean composition analysis obtained at the... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics & Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6253/version/1 |
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| Tabatha N. Offutt-Powell; Rohit P. Ojha; Joseph Tota; Karan P. Singh. |
| OBJECTIVE: To evaluate the association between having a household member with a cancer-related limitation (physical, mental, or emotional) and human papillomavirus (HPV) vaccine uptake among females aged 9 – 17 years. 
METHODS: We analyzed 2008 National Health Interview Survey for our cross-sectional analysis. All households with females aged 9 – 17 years were eligible for inclusion in our analysis. Our outcomes of interest included HPV vaccine initiation (≥1 dose) and series completion (full 3 doses). A dichotomous variable indicating a cancer-related limitation for a household member was created if at least one adult in the household responded to having a limitation caused by cancer. We used... |
| Tipo: Manuscript |
Palavras-chave: Bioinformatics. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4925/version/1 |
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| Xin Wang; Shaolin Zheng. |
| Despite of angiosperms in the Yixian Formation (>125 Ma, early Cretaceous), there is no perfect flower typical of angiosperms to date. Here we report _Euanthus dilaensis_ gen. et sp. nov as the earliest perfect flower known to date. The flower includes tepals, androecium and gynoecium. The anthers are globose in form, with bristles atop and in situ round-triangular pollen grains. The gynoecium is composed of probably two carpels with plumose stigmas and a carpel-enclosing receptacle. The discovery of _Euanthus_ increases the diversity of early angiosperms, and indicates that perfect flowers occurred as early as 125 Ma ago.
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| Tipo: Manuscript |
Palavras-chave: Ecology; Evolutionary Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1320/version/1 |
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| Flávio Codeço Coelho; Claudia Codeco; Oswaldo Cruz. |
| The construction of complex statial simulation models such as those used in network epidemiology, is a daunting task due to the large amount of data involved in their parameterization. Such data, which frequently resides on large geo-referenced databases, has to be processed and assigned to the various components of the model. All this just to construct the model, then it still has to be simulated and analyzed under different epidemiological scenarios. This workflow can only be achieved efficiently by computational tools that can automate most if not all these time-consuming tasks. In this paper, we present a simulation software, Epigrass, aimed to help designing and simulating network-epidemic models with any kind of node behavior.
... |
| Tipo: Manuscript |
Palavras-chave: Bioinformatics. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/378/version/1 |
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| Kevin Duffy. |
| Animal movements can influence their ecology and demographics. Animal movements are often characterized by path structures with directional persistence. The extent to which directional persistence improves forage success is investigated in this paper using theoretical simulations. It is shown that a movement strategy with directional persistence enables simulated animals to find more forage as compared to a random movement strategy. Situations where resources are chosen with certainty (optimally) are even more successful. Choosing resource with certainty cannot result in directional persistence. However, in cases where animals choose with certainty adjacent cells with resource but continue in their existing direction if none of these have resources then... |
| Tipo: Manuscript |
Palavras-chave: Ecology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5156/version/1 |
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| Michael E. Baker; Charlie Chandsawangbhuwana. |
| *Background.* The origins of steroid-dependent regulation of the vertebrate estrogen receptor (ER) are poorly understood. Genes with statistically significant sequence similarity to vertebrate ERs have been found in lamprey, a basal vertebrate, and amphioxus, a basal chordate. Motif analysis of these sequences provides an opportunity to investigate early events in the evolution of the ER.
*Results.* We used artificial intelligence-based software to construct twelve motifs specific to the estrogen-binding domain of ER[alpha] and ER[beta] in land vertebrates and teleosts. We mapped these ER-specific motifs onto the sequences of lamprey, amphioxus, invertebrate and selected vertebrate ERs and amphioxus, Ciona and human estrogen-related... |
| Tipo: Manuscript |
Palavras-chave: Cancer; Developmental Biology; Ecology; Evolutionary Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1542/version/2 |
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| Adeilton Brandao. |
| With the thousands of scientific papers being produced every month, picking an authors name, associate it to a research field and right evaluate his or her performance is in most of times a cumbersome task. I am proposing here that science indexes as h-index, g-index, total citations and published papers could be added to biomedical bibliographic citation in order to create a unique identifier for a given researcher. |
| Tipo: Manuscript |
Palavras-chave: Bioinformatics. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/794/version/1 |
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| Registros recuperados: 2.284 | |
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