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Bases génétiques et variabilité des caractères physiologiques impliqués dans la croissance de Crassostrea gigas ArchiMer
Baud, Jean-pierre; Palvadeau, Hubert; Nourry, Max; Haure, Joel; Penisson, Christian; Boudry, Pierre.
La production française d' huître creuse Crassostrea gigas représente, avec 144000 tonnes de production, la 4ème' place au ni veau mondial après la Chine (2 285 000 t), le Japon (225 000t.) et la Corée (1 85 000t.). Ce bivalve filtreur possède un potentiel d'adaptation élevé puisqu'on le trouve sous des latitudes très diverses. Malgré son importance économique mondiale (2 900 000 tonnes de production en 1996), à ce jour, très peu de programmes de sélection ont été mis en oeuvre sur cette espèce, qui n'est pas considérée comme domestiquées au sens génétique du terme. Toutefois, avant de lancer des programmes - nécessairement de grande ampleur - pour la sélection de souches d' huîtres creuses Crassostrea gigas, il est utile de répondre au préalable à...
Tipo: Text Palavras-chave: Génétique; Variabilité génétique; Croissance; Huîtres creuses; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00048/15881/13303.pdf
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Suivi de la reproduction de la moule (Mytilus edulis) et de l'huître creuse (Crassostrea gigas) dans le bassin de Marennes Oléron saison 2002 ArchiMer
Ducrou, John; Arnaud, Christophe.
The Charente coast has, from its northern to its southern boundary, a number of natural cupped oyster (Crassostrea gigas) collection areas interesting professionals who wish to renew their stocks. This study carried out on behalf of the oyster culture professionals and in the framework of the Ifremer/SRC contract, is entitled "Ifremer's action for the Regional Shellfish Culture Section of the Marennes-Oléron basin". It has three purposes: - Spotting larval emissions, - Monitoring the future of the larval colonies, - Enabling the providing of information to oyster professionals early enough for them to set up their collection devices. In 2002, mussel "Mytilus edulis" larvae have been collected at the "Boyard" point.
Tipo: Text Palavras-chave: Marennes Oléron; Larves; Captage; Mytilus edulis; Moules; Crassostrea gigas; Huîtres; Reproduction.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-6573.PDF
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Mass selection for survival and resistance to OsHV-1 infection in Crassostrea gigas spat in field conditions: response to selection after four generations ArchiMer
Degremont, Lionel; Nourry, Max; Maurouard, Elise.
The ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster Crassostrea gigas, and numerous mortality outbreaks have been observed worldwide. We report the first results of our experimental breeding program using mass selection focused on survival and resistance to OsHV-1 after four generations of selection for two lines. These lines originated from two stocks of adult wild oysters sampled from the Marennes-Oléron Bay in 2008. Each line was spawned in February 2009 to produce the base populations. Both lines were then either protected from OsHV-1 or tested in the field in 2009 where they were exposed to OsHV-1. For each line during 2010 to 2013, one generation per year was produced using either the survivors of the...
Tipo: Text Palavras-chave: Survival; Crassostrea gigas; Realized heritability; Selection response; OsHV-1; Disease resistance.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00266/37751/36064.pdf
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Etude in vivo et in vitro des effets du mercure sur la réponse hémocytaire de l'huître creuse, Crassostrea gigas : développement de techniques évaluant les impacts de la pollution estuarienne ArchiMer
Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
In the last decades, shellfish industry represents an important economic activity in France and particularly in Charente-Maritime. Marennes-Oleron basin is the first French area of oyster production, especially Pacific oyster, Crassostrea gigas. But this area is also subjected to many pollution due to anthropic activities. Industrial wastes are sources of mercury pollution in the Charente river. The harmful effects of such contaminants on animals inhabiting these estuarine zones are poorly known, although many studies begin to be developed. Most of these studies use bivalve molluscs as biological indicators : these animals can bioaccumulate pollutants within their tissues by filtration. Moreover, they are sedentary so they cannot escape in the case of a...
Tipo: Text Palavras-chave: Flow cytometry; Haemocytes; Mercury; Crassostrea gigas; Cytométrie de flux; Hémocytes; Mercure; Crassostrea gigas.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-830.pdf
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Size and genotype affect resistance to mortality caused by OsHV-1 in Crassostrea gigas ArchiMer
Degremont, Lionel.
As with summer mortalities reported in France between 2001 and 2006, mortality caused by the Ostreid herpesvirus 1 (OsHV-1) in Crassostrea gigas affects mostly juveniles, although adults can also be impacted to a small extent. This could suggest that both mortalities have similar causes and that establishment of resistance, in particular to the Ostreid herpesvirus 1 (OsHV-1), depends on either the size or the age of oysters. The present study reports an investigation of both size and age using three cohorts produced during winter and three produced during summer. Each cohort contained oysters genetically selected to be resistant or susceptible to the summer mortality phenomenon, as well as unselected control oysters. Any abnormal mortality was recorded...
Tipo: Text Palavras-chave: Mortality; Crassostrea gigas; Disease resistance; Size; Age; Ostreid herpesvirus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26799/24909.pdf
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Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf
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Observations préliminaires sur la reproduction des huîtres dans le bassin de Marennes-Oléron en 1971 ArchiMer
Gras, Paul; Comps, Michel; David, A.; Baron, G..
During the summer of 1970 mortality started in the Marennes-Oléron Basin which affected successively adult oysters, 18 year-old oysters and then the oyster spats collected the same year. Only the Portuguese oyster (Crassostrea angulata) was dying whereas the Pacific oyster (Crassostrea gigas) showed total resistance. The abnormal fixation of the 1970 spats, followed by an important mortality in this latter, and the large decrease of adult oyster stocks, and therefore of genitors, were also a reason for oyster farmers to worry as the new reproduction season was about to start. Some measures had to be taken in order to compensate for the lack of genitors susceptible of producing a tough and vivacious spat. The good results obtained for a few years with C....
Tipo: Text Palavras-chave: Naissain; Larves; Marennnes Oléron; Crassostrea gigas; Reproduction.
Ano: 1971 URL: http://archimer.ifremer.fr/doc/1971/publication-6446.PDF
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Ecologie alimentaire de l'huitre Crassostrea gigas : dynamiques des compositions isotopiques naturelles ArchiMer
Malet, Nathalie.
Trophic transfers of particulate organic matter sources to the Pacific oyster Crassostrea gigas were investigated in the oyster farming bay of Marennes-Oléron for two years. Stable isotope ratios of carbon and nitrogen were used to trace the origin and fate of primary production and organic matter inputs into the bay, and to depict trophic transfers to oysters that were experimentally reared according to traditional methods. Characterizing particulate organic matter sources available for oysters utilized a combination of hydrobiological parameters and stable isotope ratios. Processes of food incorporation by oysters were described for five organs such as gills, digestive gland, mantle, muscle and gonads, and were interpreted taking into account...
Tipo: Text Palavras-chave: Microphytobenthos; Phytoplankton; Organs; Trophic discrimination; Tissue turnovers; Particulate organic matter; Marennes Oléron Bay; Pertuis Charentais; Stable isotopes; Pacific oysters; Crassostrea gigas; Microphytobenthos; Phytoplancton; Hydrologie; Organes; Enrichissement trophique; Renouvellement tissulaire; Matière organique particulaire; Bassin de Marennes Oléron; Pertuis Charentais; Isotopes stables; Crassostrea gigas; Huître creuse.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2089.pdf
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A latitudinal gradient of seasonal temperature variation recorded in oyster shells from the coastal waters of France and The Netherlands ArchiMer
Lartaud, Franck; Emmanuel, Laurent; De Rafelis, Marc; Ropert, Michel; Labourdette, Nathalie; Richardson, Christopher A.; Renard, Maurice.
Cathodoluminescence (CL) microscopy of the foliated calcite shell hinge sections of live-collected oyster Crassostrea gigas collected at seven locations along a latitudinal gradient from the Netherlands in the North Sea to the Atlantic coast of France, revealed variations in luminescence that were attributable to seasonal variations in calcification of the hinge. Photomicrographs of hinge sections and luminescence profiles were analyzed to define a micro-sampling strategy that was adopted to drill the hinge samples to determine their isotopic composition. Reconstructed seasonal seawater temperatures determined from the stable oxygen isotope (delta O-18) composition along growth profiles from 32 oyster shell hinges showed distinct seasonal isotopic cycles...
Tipo: Text Palavras-chave: Seasonality; Palaeotemperatures; Stable isotopes; Cathodoluminescence; Crassostrea gigas; Mollusk shells.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2010/publication-7296.pdf
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Storage of Pacific oysters Crassostrea gigas in recirculating tank: ammonia excretion and potential nitrification rates ArchiMer
Buzin, Florence; Dupuy, Beatrice; Lefebvre, Sebastien; Barille, Laurent; Haure, Joel.
In response to the closure of shellfish production sites due to increasing occurrence of toxic algal blooms, land-based recirculating aquaculture systems (RAS) could be used by producers to store a proportion of their stock temporarily. An RAS prototype was tested in this study to store Pacific oyster Crassostrea gigas over a range of temperatures. In such systems, the water temperature is a variable that could influence the bivalve excretion rate of ammonia, the concentration of which may become critical for the water quality. In this study, we first estimated the total ammonia nitrogen (TAN, N-NH4+ + N-NH3) excretion of the Pacific oyster and then investigated whether the bacterial population associated with the oysters could act as a natural biofilter...
Tipo: Text Palavras-chave: Crassostrea gigas; Ammonia excretion; Nitrification rate; Recirculating system; Temperature.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00248/35889/34409.pdf
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Compte rendu sur la reproduction des huîtres et des moules dans le bassin de Marennes Oléron pour l'année 1985 ArchiMer
Ratiskol, Jacqueline; Heurtebise, Serge.
Au cours de l'année 1985, le laboratoire CSRU de La Tremblade a pris en charge le suivi de la reproduction des larves d'huîtres et de moules dans le bassin de Marennes Oléron. Les différents points sont choisis en fonction de critères tels que la proximité des bancs géniteurs (gisements naturels d'huîtres ou de moules) et des zones traditionnelles de reproduction des huîtres : Les Doux et Mérignac pour le bassin, Faulx, Coux et Les Roches pour la Seudre, Les Palles et La mouclière pour la Charente.
Tipo: Text Palavras-chave: Reproduction; Larves; Crassostrea gigas; Mytilus edulis; Bassin de Marennes Oléron.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00049/16073/13534.pdf
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Optimisation des conditions hydrobiologiques pour la conservation de l'huître creuse Crassostrea gigas en système re-circulé ArchiMer
Buzin, Florence.
The maintenance of bivalve commercialisation du ring periods when sales are prohibited represents a major economic issue for shellfish-farming. To ensure their survival and to preserve the oyster Crassostrea gigas quality, their storage in land-based systems has been studied i) out of water in a cold room at 3°C with controlled humidity, enabling the bivalve sensory quality to be retained for 15 days, and ii) in recirculating systems in immersed conditions for 30 days. For the latter, the oysters were stored at 167 kg.m3 density in a tank equipped with an air-lift system, with water treated with UV and thermoregulated. A minimum of food was supplied to avoid mass tissue loss. A temperature of 16°C was defined as optimal to limit mortality and to avoid...
Tipo: Text Palavras-chave: Crassostrea gigas; Huître Pacifique; Système re-circulé; Ecophysiologie; Sauvegarde; Modélisation; Crassostrea gigas; Pacific oyster; Recirculating system; Ecophysiology; Safeguard; Modelling.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00092/20350/17995.pdf
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Mortalité de Crassostrea gigas dans le bassin de Marennes Oléron. Etude physico chimique du sédiment. Etude du modèle de mortalité "plat-table" de C. gigas dans le bassin de Marennes Oléron. Etude "Dynamo" du projet MOREST ArchiMer
Soletchnik, Patrick; Bouchet, Vincent; Malestroit, Pascal; Seugnet, Jean-luc; Blouin, Frederic; Radford-knoery, Joël; Burgeot, Thierry; Sauriau, Pierre-guy.
In the scope of the study of the "on-bottom/off-bottom" mortality model in the south of the Marennes Oléron Bay, the result of the 2003 multidisciplinary study (Dynamor) appears to be the hypothesis of an acute stress, "ultimate" trigger of the mortality episodes in June. In 2004 we aim to look for the nature of this stress through the physico-chemical analysis of the sediment (Eh, pH, sulphides and ammoniacal nitrogen), the organic matter and chlorophyll load on the surface of the sediment between April and July. Herbicides are also measured in the water column, south of the bay (Seudre), during the same period. A "precocious" (around the first week of June) and "weak" (lower mortality rate on this working site since 1997) mortality peak happens when the...
Tipo: Text Palavras-chave: Bassin Marennes Oléron; Phytobenthos; Mortalité; Crassostrea gigas; Foraminiferes benthiques; Répartition verticale; Sédiment; Huître; Echantillonnage.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/rapport-3383.pdf
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Effects of twelve pesticides on larvae of oysters (Crassostrea gigas) and on two species of unicellular marine algae (Isochrysis galbana and Chaetoceros calcitrans) ArchiMer
His, Edouard; Seaman, Matthias.
The effects of seven herbicides, four insecticides and one molluscicide were tested at concentrations of up to 10 mg/l on larvae of oysters, Crassostrea gigas (9 days exposure), and on laboratory cultures of the algae Isochrysis galbana and Chaeloceros calcilrans (21 days exposure). Ali of the pesticides tested had significant toxic effects on at least one of the test organisms. The strongest effects were those of lindane and isoproturon on survival and growth of C.gigas larvae, and of isoproturon and carbetamide on growth of Isochrysis and Chaeloceros cultures. The log-probit model for the relationship between dosage and effect, and Haber's rule for the relationship between duration of exposure and effect, hardly ever applied to our data. In sorne cases...
Tipo: Text Palavras-chave: Oysters; Crassostrea gigas; Insecticides; Herbicides; Isochrysis galbana; Chaetoceros calcitrans.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00018/12949/9922.pdf
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Mortalités estivales de l'huître Crassostrea gigas dans le bassin d'Arcachon : facteurs du milieu, aspects biochimiques et histologiques ArchiMer
Maurer, Daniele; Comps, Michel.
Previous pathological studies have not permitted to implicate infectious disease in summer mortalities of oysters Crassostrea gigas, in the Arcachon bay. In 1983, these summer mortalities, occurring mostly among 1 year old oysters, have been the subject of new investigations. In order to establish the causes of mortalities, oysters of the same origin were held in two areas characterized by different prevalence of mortality for 6 months, during which measurements of physiological and biochemical condition and certain histological observations were made. The study has been completed by environmental observations. Different rates of mortality appeared between the two selected areas: 30 %in Taussat in the upper part of the bay, under a continental influence...
Tipo: Text Palavras-chave: Histology; Biochemistry; Environment; Summer mortality; Crassostrea gigas; Histologie; Biochimie; Environnement; Mortalité estivale; Crassostrea gigas.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/publication-4317.pdf
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Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas ArchiMer
Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre.
Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program...
Tipo: Text Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf
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Transcriptomic study of 39 ostreid herpesvirus 1 genes during an experimental infection ArchiMer
Segarra, Amelie; Faury, Nicole; Pepin, Jean-francois; Renault, Tristan.
Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression. Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post injection. Several...
Tipo: Text Palavras-chave: Crassostrea gigas; OsHV-1; Viral gene expression; Inhibitors of apoptosis; Real time PCR; Elongation factor.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29549/27875.pdf
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Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas ArchiMer
Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc.
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total...
Tipo: Text Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00239/35010/33544.pdf
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Effect of nutrition on Crassostrea gigas larval development and the evolution of physiological indices Part B: Effects of temporary food deprivation ArchiMer
Ben Kheder, Rym; Quere, Claudie; Moal, Jeanne; Robert, Rene.
In the natural environment bivalve larvae are exposed to variable conditions and can therefore face periods of food shortage. To understand the resistance of early life stages of Pacific oyster (Crassostrea gigas), larvae were experimentally starved for 4 days at different periods of their development, and the resulting variations in their lipid reserves then analysed using image analysis and biochemical techniques. Faced with a temporary lack of food, the larvae halted their growth in size and weight and started to live off their lipid reserves; this effect was highly marked when the period of food deprivation was at the beginning of larval rearing, but was less marked when food deprivation was after day 14. The larvae conserved their developmental...
Tipo: Text Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Starvation; Lipids.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12906/10145.pdf
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Experimental transmission of a Herpes-like virus to axenic larvae of Pacific oyster, Crassostrea gigas ArchiMer
Le Deuff, Rose-marie; Nicolas, Jean-louis; Renault, Tristan; Cochennec, Nathalie.
A herpes-like virus which was associated with mortalities amongst hatchery-reared larvae of Pacific oysters, Crassostrea gigas. was observed for the first time in France during the summer 1991 (Nicolas et al.. 1992). Since this first report, we have noted further outbreaks of the disease in the summers of 1992 and 1993 in hatcheries on the western coasts of France in which a similar virus was associated with moribund larvae. Moreover, in the summer 1991, Hine et al. (1992) described a herpes-like virus responsible for larval mortality of hatchery reared C. gigas, in New Zealand. Ail these viruses are c10sely related with respect to their size and morphology and to their apparent cellular tropism for fibroblastic cells.
Tipo: Text Palavras-chave: Transmission; Herpes like virus; Crassostrea gigas; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-2887.pdf
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