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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Approche de la capacité trophique dans un bassin ostréicole (Baie de Bourgneuf) ArchiMer
Haure, Joel.
Une étude spatio-temporelle a été menée de mars 1990 à mars 1991 sur deux stations ostréicoles (nord et sud), de la baie de Bourgneuf. Cette étude se divise en deux parties distinctes : - L'une, hydrobiologique a permis, par des prélèvements hebdomadaires à basse mer plus trois heures, de caractériser les masses d'eau qui baignent chaque point d'étude, - L'autre, a eu pour but d'observer, par un suivi mensuel, l'évolution de la croissance, de l'engraissement et de la mortalité d'un échantillon d'huîtres (Crassostrea gigas) élevé sur chacun des secteurs; L'examen des résultats obtenus amène aux constatations suivantes :  - Le secteur nord présente un milieu hétérogène d'une richesse nutritive importante mais est accompagnée d'une charge sestonique minérale...
Tipo: Text Palavras-chave: Baie de Bourgneuf; Crassostrea gigas; Seston; Météorologie; Hydrobiologie; Croissance; Mortalité; Capacité trophique.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00449/56095/57630.pdf
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Pacific cupped oyster - Crassostrea gigas ArchiMer
Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe.
Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3).
Tipo: Text Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf
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ReMoNor : Résultats 2000 ArchiMer
Cornette, Florence; Blin, J.l.; Pacary, S; Pien, S; Le Gagneur, Eric; Kopp, Joel; Richard, O.
At the end of its third year, the regional network for monitoring REMONOR, confirms the particularities of oyster from Normandy in matter of growth and quality. Results from 2 000 are globally very close of those from 1998. Mortality of adults reached 14% and juvenile 6% which represent a low death rate. For this year the most blow area is the Baie des Veys with 31% death rate at Géfosse. Observed growth this year had been weaker than the past two years despite the spring and automnal growth. At spring, the higher recorded growth rate on the West coast with 0.42% (regional average : 0.29%). This seasonal trend seems more and more obvious every year. Regarding the classification of the special oysters, despite the change of the standard, the Baie des Veys...
Tipo: Text Palavras-chave: REMORA; Quality; Growth; Mortality; Normandy; REMONOR; Crassostrea gigas; REMORA; Qualité; Croissance; Mortalité; Basse Normandie; REMONOR; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/rapport-2251.pdf
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Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm ArchiMer
Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P..
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf
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Intensive rearing of juvenile oysters Crassostrea gigas in an upwelling system : optimization of biological production ArchiMer
Bacher, Cedric; Baud, Jean-pierre.
Seasonal growth of juvenile oysters (Crassostrea gigas) kept in an intensive upwelling system was studied from 1984 to 1986 in 60 to 90-day growth experiments. Saline ground water was used to produce Skeletonema costatum which was injected as food into the water supplying the upwelling system. Oyster density, water flow, phytoplankton concentration, temperature and frequency of food addition were controlled. Oyster growth was analysed with multidimensional contingency tables and correspondence analysis. The factors, in order of decreasing influence on growth, were temperature, food concentration and oyster density. A rearing strategy was deduced from the growth analysis for summer and winter. In summer, the density of 120,000 oysters/m2, the flow rate of...
Tipo: Text Palavras-chave: Nursery; Growth; Correspondence analysis; Multidimensional contingency table analysis; Economic efficiency; Skeletonema costatum; Crassotrea gigas; Nourriture; Croissance; Analyse des correspondances; Table de contingence multidimensionnelle; Rentabilité économique; Skeletonema costatum; Crassostrea gigas.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-3061.pdf
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Genetic effect of domestication selective pressures on Pacific oyster at larval stage ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf
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Affinage contrôlé en bassin de l'huître creuse Crassostrea gigas ArchiMer
Baud, Jean-pierre; Brisset, Emmanuelle; Cardinal, Mireille.
Une étude d'affinage contrôlé en bassin de l'huître creuse Crassas/rea gigas a été réalisée en novembre 1991 par "IFREMER de Bouin (Vendée). Cinq tonnes d'huîtres ont été nourries avec la diatomée Skeletonema costatum produite sur eau salée souterraine à raison de 1,2 X 10(9) cellules/individus/jour. L'affinage a été réalisé dans un bassin bétonné de 500 m2 muni d'une rampe de bullage d'air à une densité de 11 kg/m2 de coquillages. L'influence de différents paramètres sur l'affinage a été testée à l'aide d'un plan factoriel incluant : La taille des huîtres (86,8g ± 3,8 ; 69,5g ± 3,2 ; 53,1g.± 2,3), la technique de stockage (au sol, en casier surélevé, en poche surélevée) et la situation des huîtres par rapport à l'injection du phytoplancton (alimentation,...
Tipo: Text Palavras-chave: Affinage contrôlé; Crassostrea gigas; Skeletonema costatum; Glycogène.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00048/15882/13304.pdf
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Développement d'ou outil intégré de gestion spécialisée (Base de données/SIG) des stocks conchylicoles dans les Pertuis Charentais ArchiMer
Le Moine, Olivier.
Estimating farmed shellfish stocks is essential, in terms of both developing shellfish farms and evaluating trophic capacities with respect to existing stocks. At Marennes-Oléron, estimation is carried out every five years, following a multi-layered strategy. First, cluster sampling of areas of 1 ha provided an estimate of biomass per surface area as well as characteristics of oyster farms (age, type of collectors used, etc.). These data were then interpolated to the stratum level. Second, the overall exploitation rate of the stratum was estimated based on coverage by aerial photographs. Both types of data have been processed using special statistical software developed using a database and Kman language (SGBD; Maurer, 1989). Photos were analysed under a...
Tipo: Text Palavras-chave: Marennes Oléron; Pertuis Charentais; Crassostrea gigas; Huîtres; Estimation stocks; SIG.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3264.pdf
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Mn labelling of living oysters: Artificial and natural cathodoluminescence analyses as a tool for age and growth rate determination of C. gigas (Thunberg, 1793) shells ArchiMer
Lartaud, Franck; De Rafelis, Marc; Ropert, Michel; Emmanuel, Laurent; Geairon, Philippe; Renard, Maurice.
We developed a growth model for Crassostrea gigas oyster shells based on the use of in situ temporal manganese markings to calibrate natural cathodoluminescence (CL) changes in the shell hinge sections. A 30 min to 4-h exposure period with Mn2+ (90-120 mg l(-1)) was sufficient to create a detectable mark in the shells. This makes the Mn2+ markings the fastest mollusc shells marking technique to date. The natural CL from juvenile and adult shells cultured in four standard shellfish-farming locations along the English Channel and French Atlantic coasts, exhibited a seasonal pattern (maximum CL intensity occurring during summer periods, minimum CL intensity occurring during winter). Hydrobiological data recorded at Baie des Veys site allows us to attribute...
Tipo: Text Palavras-chave: Oyster shells; Crassostrea gigas; Manganese markings; Cathodoluminescence; Seasonal growth patterns.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11300/7912.pdf
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Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds ArchiMer
Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan.
Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster...
Tipo: Text Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf
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Effets des sels organiques de tributyletain sur l'huître adulte Crassostrea gigas ArchiMer
Heral, Maurice; Alzieu, Claude; Caux, O.; Razet, Daniel; Garnier, Jacqueline.
The toxicity of organostannic compounds has been demonstrated for the oyster Crassostrea gigas through larval (His & Robert, 1980) and adult stages (Alzieu & al., 1980, 1981). In parallel, these authors demonstrated the accumulation of tin in experimentally contaminated oysters as well as the part played by TBT fluorides in growth disturbance (Héral & al. 1981) and oyster shell deformity (Alzieu & al., 1981). In this work, we precise the TBT fluoride toxic doses causing, after 35 days, a mortality of 100 % at a concentration of 0.5 µgl-1. In addition, the different TBT organic salts (acetate, methacrylate and fluoride) are tested and TBT fluoride appears to be the more toxic. Authors then tackle breathing and ATP measurements as stress...
Tipo: Text Palavras-chave: Tributyletain; Crassostrea gigas; Toxicité.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-6216.PDF
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Rapport provisoire estimation et gestion des stocks conchylicoles. Richesse nutritive des secteurs de production de la Baie de Bourgneuf en 1987 ArchiMer
Baud, Jean-pierre; Haure, Joel.
Ce rapport provisoire présente les premiers résultats des études financées par le SMIDAP , (arrêté 87.4.224) sur la Baie de Bourgneuf, pour l 'année 1987 . Il traite donc de l'estimation des stocks conchylicoles et de la richesse nutritive des secteurs de production ostréicole de cette baie.
Tipo: Text Palavras-chave: Ostréiculture; Estimation de stocks; Biomasse; Crassostrea gigas; Baie de Bourgneuf.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00048/15893/13315.pdf
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Evaluation des Risques dus a l'emploi des Peintures Anti-Salissures Dansales Zones Conchylicoles. ArchiMer
Alzieu, Claude; Thibaud, Yves; Heral, Maurice; Boutier, Bernard.
The boats cruising in the oyster growing areas are protected against biofouling by paints which release toxic compounds such as copper oxide and organotins into the water. After an estimation of the inputs of copper and organotins in the "Marennes-Oleron", Arcachon (Atlantic) and Thau (Mediterranean Sea) areas, their influence on larval fixation is discussed, taking into account the tributyltin toxicity against D larvae of Crassostrea gigas. Laboratory experiments have demonstrated that tributyltin produces morphological abnormalities of the shell and increases tin levels in tissues, interfering with copper and zinc metabolism. The influence of yacht ports on oyster growth and shell morphology, has been demonstrated in the field. The use of...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster culture; Abnormalities; Shells; Growth; Paints; Antifouling substances; Pollution effects.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/publication-1905.pdf
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Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses ArchiMer
Dupont, Samuel; Lokmer, A.; Corre, E.; Auguet, Jean-christophe; Petton, Bruno; Toulza, E.; Montagnani, Caroline; Tanguy, G.; Pecqueur, D.; Salmeron, C.; Guillou, L.; Desnues, C.; La Scola, B.; Bou Khalil, J.; De Lorgeril, Julien; Mitta, Guillaume; Gueguen, Yannick; Escoubas, Jean Michel.
Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a...
Tipo: Text Palavras-chave: Oyster genetic background; Hemolymph microbiota dynamics; Early-life microbiota; Trans-kingdom interactions; Crassostrea gigas; Within-host ecosystem.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00627/73916/73254.pdf
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L'huître creuse Crassostrea gigas en Bretagne. Résultats du réseau de suivi de 1990 ArchiMer
Le Bec, Claude; Mazurie, Joseph.
The year 1990 is the second of a regional monitoring program on production assessment of Crassostrea in Britlany. 9 sites have been tested with 3 stations per site and 3 oysters bags in each station, at 3 different densities : 170, 210 and 250 oysters per bag. 18 month old oysters stocked in bags in march were analyzed 43 weeks later, by variables concerning growth, survival, quality... The growth was lower at higher densities, more or less according to sites. As previously, differences between sites and stations are observed, with some confirmations (higher mortalities in Aber Benoît site) and also evolutions (growth and Polydora infestations improvement in Pen Bé site).
Tipo: Text Palavras-chave: Bretagne; Crassostrea gigas; Mortalité; Indice de condition; Polydora; ACP; Brittany; Crassostrea gigas; Mortality; Condition index; Polydora; Factorial analysis.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00102/21318/18938.pdf
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Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns ArchiMer
Bouilly, Karine; Chaves, R.; Leitao, A.; Benabdelmouna, Abdellah; Guedes Pinto, H..
Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed...
Tipo: Text Palavras-chave: Simple sequence repeats (SSRs); Mollusks; Fluorescence in situ hybridization (FISH); Crassostrea gigas; Chromosome identification.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6132.pdf
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Contribution à l'étude de l'ontogenèse du système immunitaire chez l'huître creuse Crassostrea gigas ArchiMer
Tirape Bajana, Ana.
The interest for the understanding of the immune mechanisms in the oyster, Crassotrea gigas has been considerably increased these last years, mainly because of economie losses due to diseases which affected the continuity of this species. The studies of these mechanisms contributes to a better understanding of the differential susceptibilities observed in this species against various pathogens. In the present study, our  goal was to define the ontogenesis of immune system in C. gigas during the development, by functional genomic approaches. The studies have been focalized on the analyses of expression and localization of effectors previously identified in adult oysters as implicated in immunity. We have shown, in particular, that hemocytes appear in the...
Tipo: Text Palavras-chave: Invertébré; Mollusque bivalve; Huître; Cra; Immunité; Hémocytes; Hématopoïèse; Développement; Ontogenèse; Expression des gènes; Q-PCR; SSH; Invertebrate; Bivalve Mollusk; Oyster; Crassostrea gigas; Immunity; Hemocytes; Hematopoiesis; Development; Ontogenesis; Gene expression; Q-PCR; SSH.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00440/55113/56565.pdf
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Ecophysiologie de coquillages contaminés par des microalgues toxiques : premiers essais ArchiMer
Rohmer, Véronique.
Afin d'évaluer les effets d'une contamination expérimentale par des microalgues toxiques sur la filtration,· des bivalves (Pecten maximus, Crassostrea gigas et Mytilus edulis) ont été alimentés pendant quelques heures avec différents régimes : des cultures très toxiques (Alexandrium tamarense, souche japonaise), moyennement toxique (A. minutum, souche française) et non toxique (A. tamarense, souche anglaise) de dinoflagellés, et une culture non toxique témoin d'une chlorophycée : Tetraselmis suesica. Les différents essais réalisés montrent que l'utilisation de rouge neutre ne permet pas de comparer les activités valvaires dans les conditions expérimentales. L'enregistrement direct de l'activité valvaire est intéressant mais difficilement reproductible dans...
Tipo: Text Palavras-chave: Pecten maximus; Mytilus edulis; Crassostrea gigas; Ecophysiologie; Microalgues toxiques; Paralytic Shellfish Poison.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00429/54043/55314.pdf
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Echantillonnage des populations naturelles de mollusques compétiteurs trophiques des huîtres cultivées Crassostrea gigas du bassin de Marennes-Oléron : aspects méthodologiques ArchiMer
Sauriau, Pierre-guy.
The main problems involved in making biomass and density estimates of benthic mol1uscs are the choice of a sampling plan, the level of systematic errors and random errors. A stratified random sampling after optimum allocation was used in the case. The benthic molluscs estimates (biomass or density) are highly variable, due to their aggregated distribution. The sampling gear used is a Smith Mc-lntyre grab which introduce a bias in the estimates. Nevertheless, the Macoma baLthica community was predominant in the bay but Cerastoderma edul.e appeared to be the dominant specie.
Tipo: Text Palavras-chave: Cerastoderma edule; Macoma balthica; Marennes Oleron Bay; Crassostrea gigas; Stock assessment; Cerastoderma edule; Macoma balthica; Marennes Oléron; Crassostrea gigas; Echantillonnage.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-2971.pdf
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