|
|
|
| Registros recuperados: 179 | |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre. |
| Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of... |
| Tipo: Text |
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf |
| |
|
| |
|
|
| Strong, Ellen E.; Bouchet, Philippe. |
| A new genus, Limatium gen, n., and two new species, L. pagodula sp, n, and L. aureum sp, n. are described, found on outer slopes of barrier reefs and fringing reefs in the South Pacific. They are rare for cerithiids, which typically occur in large populations. The two new species arc represented by 108 specimens sampled over a period of 30 years, only 16 of which were collected alive. Three subadults from the Philippines and Vanuatu likely represent a third species. In addition to their rarity, Limatium species arc atypical for cerithiids in their smooth, polished, honey to golden brown shells with distinctive white fascioles extending suture to suture. The radula presents a unique morphology that does not readily suggest an affinity to any of the... |
| Tipo: Text |
Palavras-chave: Bittiinae; New genus; New species; Marine; DNA. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00494/60615/64101.pdf |
| |
|
|
| Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan. |
| Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific... |
| Tipo: Text |
Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf |
| |
|
|
| Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.. |
| The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and... |
| Tipo: Text |
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf |
| |
|
|
| Lazerges, M; Perrot, H; Antoine, Elisabeth; Defontaine, Audrey; Compere, Chantal. |
| We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes... |
| Tipo: Text |
Palavras-chave: DNA; Alexandrium minutum; Microbalance. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1354.pdf |
| |
|
| |
|
| |
|
|
| Poirier, Aurore C.; Schmitt, Paulina; Rosa, Rafael D.; Vanhove, Audrey S.; Kieffer-jaquinod, Sylvie; Rubio, Tristan P.; Charriere, Guillaume M.; Destoumieux-garzon, Delphine. |
| Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial... |
| Tipo: Text |
Palavras-chave: Antimicrobial Peptide (AMP); DNA; Innate Immunity; Invertebrate; Reactive Oxygen Species (ROS); NET; Mollusk. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00217/32866/31784.pdf |
| |
|
|
| Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal. |
| We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA... |
| Tipo: Text |
Palavras-chave: QCM; DNA; Biosensor. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf |
| |
|
| |
| Registros recuperados: 179 | |
|
|
|
