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Sprint swimming performance of juvenile European sea bass ArchiMer
Nelson, J; Claireaux, Guy.
Despite its potential for determining animal success in a variety of ecologically important situations, very little is known about the sprint swimming performance of fishes. To evaluate whether selection on sprint swimming performance occurs in the wild, one must first demonstrate measurable and reproducible variation of sprint performance. In this study, we employ a computer-controlled laser detection system to study the sprint performance of 75 juvenile European sea bass Dicentrarchus labrax raised under uniform conditions in the laboratory. This percichthyid is a prized sport and food fish in Europe and similar ecologically to the North American striped bass Morone saxatilis. Individual European sea bass were subjected to endurance swimming performance...
Tipo: Text Palavras-chave: Dicentrarchux labrax; Juveniles; Computer laser detection; Endurance; Metabolism; Swimming performance; Fish.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-887.pdf
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Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium. ArchiMer
Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf
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Models projecting the fate of fish populations under climate change need to be based on valid physiological mechanisms ArchiMer
Lefevre, Sjannie; Mckenzie, David J.; Nilsson, Goran E..
Some recent modelling papers projecting smaller fish sizes and catches in a warmer future are based on erroneous assumptions regarding (i) the scaling of gills with body mass and (ii) the energetic cost of 'maintenance'. Assumption (i) posits that insurmountable geometric constraints prevent respiratory surface areas from growing as fast as body volume. It is argued that these constraints explain allometric scaling of energy metabolism, whereby larger fishes have relatively lower mass-specific metabolic rates. Assumption (ii) concludes that when fishes reach a certain size, basal oxygen demands will not be met, because of assumption (i). We here demonstrate unequivocally, by applying accepted physiological principles with reference to the existing...
Tipo: Text Palavras-chave: Aerobic scope; Gill surface area; Growth; Metabolism; Oxygen consumption; Respiration; Scaling.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00395/50621/51449.pdf
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Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas ArchiMer
Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Brown, Craig; Cragg, Simon.
The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiological modelling. As an example of the application of this approach, additional measurements of veliger respiratory rates were carried out to assess the effect on larval metabolism of different concentrations of leachate from wood treated with chromated copper-arsenate (CCA). Veligers of Crassostrea gigas (length from 95 to 331 pm) were fed with a mixture of Isochrysis galbana) and Chaetoceros pumilum cells. Experiments were performed in a 20 degreesC constant...
Tipo: Text Palavras-chave: Crassostrea gigas; Leachate; CCA; Volumetric micro respirometry; Larvae; Metabolism; Respiratory rate.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-399.pdf
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa ArchiMer
Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky.
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J...
Tipo: Text Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf
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Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host ArchiMer
Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf
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Chitin/toxicant interactions and microbial degradation within estuarine ecosystems. ArchiMer
Portier, R; Meyers, S.
The environmental effect and fate of several agricultural and industrial source toxicants were evaluated in controlled laboratory microcosm systems in the presence of the aminopolysaccharide polymer, chitin. Toxicants examined included methyl parathion, phenol, pentachlorophenol, 2,4 dichlorophenol, 1,2 dichloroethane and Kepone. Microcosm operating parameters, including pH/Eh, salinity, temperature, flow rate and sediment/water interface conditions, were based on comparable in situ measurements. Fate analysis predictions were based on mineralization of the parent toxicant compound as measured by chromatographic analysis or super(14)CO sub(2) expiration. Chitin, amended to continuous flow microcosms, promoted either cometabolic or cooxidative...
Tipo: Text Palavras-chave: Controlled conditions; Chemical pollutants; Industrial wastes; Pesticides; Metabolism; Food webs; Biodegradation; Ecosystems; Microorganisms; Environmental effects; Brackishwater pollution; Estuaries; Toxicants; Chitin.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1011.pdf
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Reconstructing individual food and growth histories from biogenic carbonates ArchiMer
Pecquerie, Laure; Fablet, Ronan; De Pontual, Helene; Bonhommeau, Sylvain; Alunno-bruscia, Marianne; Petitgas, Pierre; Kooijman, Sebastiaan A. L. M..
Environmental conditions experienced by aquatic organisms are archived in biogenic carbonates such as fish otoliths, bivalve shells and coral skeletons. These calcified structures present an accretionary growth and variations in optical properties - color or opacity - that are used to reconstruct time. Full and reliable exploitation of the information extracted from these structures is, however, often limited as the metabolic processes that control their growth and their optical properties are poorly understood. Here, we propose a new modeling framework that couples both the growth of a biogenic carbonate and its optical properties with the metabolism of the organism. The model relies on well-tested properties of Dynamic Energy Budget (DEB) theory. It is...
Tipo: Text Palavras-chave: Otolith; Calcification; Metabolism; Bioenergetic model; Food reconstruction; Dynamic Energy Budget theory.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00060/17082/14593.pdf
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Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters ArchiMer
Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf
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Effects of Heating on Proportions of Azaspiracids 1–10 in Mussels ( Mytilus edulis ) and Identification of Carboxylated Precursors for Azaspiracids 5, 10, 13, and 15 ArchiMer
Kilcoyne, Jane; Mccarron, Pearse; Hess, Philipp; Miles, Christopher O..
Azaspiracids (AZAs) are marine biotoxins that induce human illness following the consumption of contaminated shellfish. European Union regulation stipulates that only raw shellfish are tested, yet shellfish are often cooked prior to consumption. Analysis of raw and heat-treated mussels (Mytilus edulis) naturally contaminated with AZAs revealed significant differences (up to 4.6-fold) in AZA1–3 (1–3) and 6 (6) values due to heat-induced chemical conversions. Consistent with previous studies, high levels of 3 and 6 were detected in some samples that were otherwise below the limit of quantitation before heating. Relative to 1, in heat-treated mussels the average (n = 40) levels of 3 (range, 11–502%) and 6 (range, 3–170%) were 62 and 31%, respectively. AZA4...
Tipo: Text Palavras-chave: Azaspiracid; Decarboxylation; Hydroxylation; Chemical conversion; Heating mass spectrometry; Metabolism.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00303/41433/41005.pdf
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Métabolisme et interactions bactériennes en lien avec la vitamine B12 chez la microalgue haptophyte Tisochrysis lutea ArchiMer
Nef, Charlotte.
Vitamin B12 is an enzymatic cofactor that enables methionine synthesis and whose availability can control phytoplankton development in marine systems. This vitamin is synthesized only by select species of bacteria and archaea. Haptophyte microalgae form a widespread group with major ecological importance. However, little is known about their utilization of vitamin B12 nor their interactions with B12 producers. Our main objectives were to characterize mechanisms of acquisition and utilization of vitamin B12 by Haptophytes and the effect of B12 limitation on their metabolism by considering the model species Tisochrysis lutea. Analysis of the repartition of B12 dependency in haptophytes by molecular approaches suggest that this group would be the first to...
Tipo: Text Palavras-chave: Métabolisme; Vitamine B12; Interactions microbiennes; Microalgues haptophytes; Metabolism; Vitamin B12; Microbial interactions; Haptophyte microalgae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/71003/69268.pdf
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Variations of zooplankton metabolism and feeding in the frontal area of the Alboran Sea (western Mediterranean) in winter ArchiMer
Gaudy, R; Youssara, F.
Respiration, excretion (NH4 and PO4) and feeding of zooplankton from the upper 200 m of the Almeria-Oran frontal region (western Mediterranean), were studied during winter 1997. Eight sites belonging to the three main hydrodynamic structures of the area (Mediterranean water, geostrophic Atlantic jet and associated anticyclonic gyre) were considered. Lowest values of respiration, PO4 excretion, O/P atomic ratio and particle ingestion were found in Mediterranean waters. At the frontal limit, respiration and ammonium excretion were enhanced compared to the adjacent structures (Mediterranean water and Atlantic jet). In the gyre water, lowest O/N and highest N/P values were observed and maximum faecal pellet production and reproduction activity (inferred from...
Tipo: Text Palavras-chave: Mer d'Alboran; Front; Zooplancton; Métabolisme; Nutrition; Alboran sea; Front; Zooplankton; Metabolism; Feeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43275/43006.pdf
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Effects of water viscosity upon ventilation and metabolism of a flatfish, the common sole Solea solea (L.) ArchiMer
Couturier, Christine; Rouault, Alice; Mckenzie, David; Galois, Robert; Robert, Serge; Joassard, Lucette; Claireaux, Guy.
The French Atlantic coast contains large highly productive intertidal mudflats that are colonised by juveniles of numerous flatfish species, including the common sole (Solea solea, L.). These ecosystems are also heavily exploited by the shellfish farming industry. Intensive bivalve culture is associated with substantial biodeposition (1-6 t-dw ha(-1) day(-1)), which directly or indirectly contributes to increase exopolysaccharide (EPS) concentrations at the interface between water column and seabed. EPS are long-chain molecules organised into colloids, which influence rheological properties of water, particularly viscosity. Increased water viscosity had consequences for ventilatory activity of juvenile flatfish, whereby the minimal pressure required to...
Tipo: Text Palavras-chave: EPS; Hypoxia; Metabolism; Water viscosity; Biodeposition; Solea solea.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2801.pdf
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Role of bacteria in the gastral cavity of Anthozoa. ArchiMer
Herndl, G; Velimirov, B.
Bacterial density of the coelenteric fluid (CF) of some anthozoan species was determined in situ and under laboratory conditions. In all species examined bacterial density of the CF was higher in the gastral cavity than in ambient ranging from 10 super(5) up to 5 x 10 super(6) cells. ml super(-1). Incubation experiments with the CF indicate that a bacterial population within the coelenteron is efficiently controlled by the CF, favoring bacterial growth if bacterial densities are low in the CF and showing clearance activity at high bacterial densities. Feeding of pre-starved anthozoans caused a rapid response of coelenteric rod-shaped bacteria. Evidence is presented that coelenteric bacteria are digested periodically although they contribute 1% to the...
Tipo: Text Palavras-chave: Anthozoa; Coelomic fluids; Bacteria; Microorganisms; Interspecific relationships; Metabolism; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-989.pdf
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Effects of repeated hypoxic shocks on growth and metabolism of turbot juveniles ArchiMer
Person, Jeannine; Lacut, Anne-sophie; Le Bayon, Nicolas; Le Roux, Annick; Pichavant, Karine; Quemener, Loic.
Turbot juveniles (45 g) were exposed for 41 d (17 C, 34parts per thousand salinity) to constant normoxic (100-100% air saturation, 100-100) or moderate hypoxic (75-75% air saturation, 75-75) conditions and to repeated hypoxic shocks (20% saturation for 1 h, 5 d per week) from normoxic (100-20% air saturation. 100-20) or moderate hypoxic (75-20% air saturation, 75-20) conditions. A normoxic group was feed restricted (100-FR). Mass increase of 100-100 and 75-75 groups fed to satiation was not significantly different. In comparison, it was significantly lower in the 100-20 and 75-20 groups (NS between the two hypoxic shocks groups). Intermediate results were obtained in the 100-100-FR group. The lowest mass increase under hypoxic shocks was explained by a...
Tipo: Text Palavras-chave: Turbot; Metabolism; Growth; Hypoxic shock.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-571.pdf
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L'absorption intestinale des lipides chez la truite arc-en-ciel (Salmo gairdneri ) ArchiMer
Vernier, J; Sire, M.
Though the absorption of dietary lipids is much slower in S. gairdneri than in mammals, the mechanism does not differ fundamentally. The long chain fatty acids resulting from intraluminal hydrolysis are esterified by the epithelial cells and transferred as VLDL-like particles and chylomicrons to lymph. The size of granules depends on the nature of the ingested fatty acids. The intracellular stages of this lipoprotein synthesis are defined.
Tipo: Text Palavras-chave: Salmonidae; Pisces; Lymph; Fatty acids; Nutrition; Lipoproteins; Lipids; Intestines; Food absorption; Metabolism.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1245.pdf
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Metabolism of the Pacific oyster, Crassostrea gigas , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1 ArchiMer
Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice.
The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and...
Tipo: Text Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf
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Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system. ArchiMer
Velimirov, B; Herndl, G; Kavka, G.
The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...
Tipo: Text Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf
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Allelochemicals from Alexandrium minutum induce rapid inhibition of metabolism and modify the membranes from Chaetoceros muelleri ArchiMer
Long, Marc; Tallec, Kevin; Soudant, Philippe; Le Grand, Fabienne; Donval, Anne; Lambert, Christophe; Sarthou, Geraldine; Jolley, Dianne F.; Hegaret, Helene.
Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition,...
Tipo: Text Palavras-chave: Allelopathy; Alexandrium; Metabolism; Photoinhibition; Lipids; Pigments.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00460/57133/59748.pdf
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