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Registros recuperados: 90 | |
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Zhang,Yu; Zheng,Wei; Gu,Jian-Fei; Ni,Jian; Wang,Ling; Tang,Zhen-Xing; Shi,Lu-E. |
ABSTRACTIn this work, the mixture of alginate and soy protein isolate used as a wall material was developed to encapsulateEnterococcus faecalis HZNU P2 (E. faecalis HZNU P2). The survival ability in the simulated gastric fluid (SGF) and bile salt solution, storage stability at different temperatures and release properties in the simulated intestinal fluid (SIF) of encapsulated cells were assessed. The results showed that encapsulation could offer sufficient protection toE. faecalis HZNU P2. The viability of encapsulatedE. faecalis HZNU P2 did not decrease in SGF at pH 2.5 or 2.0 after 2 h incubation, while free cells were reduced from 11 to 9.85 log CFU/mL in SGF (pH 2.5) at the same exposure time. Only minor viability of encapsulatedE. faecalis HZNU P2... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecalis; Encapsulation; Soy protein isolate; Alginate; Stability; Viability. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500805 |
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Salmon,Denise Naomi; Piva,Luíza Cesca; Binati,Renato Leal; Vandenberghe,Luciana Porto de Souza; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Spier,Michele Rigon. |
A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phytase; Shizophyllum sp; Downstream steps; Stability; Formulation. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600001 |
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Ito,Vivian Cristina; Zielinski,Acácio Antônio Ferreira; Demiate,Ivo Mottin; Spoto,Marta; Nogueira,Alessandro; Lacerda,Luiz Gustavo. |
Abstract The effects of gamma radiation (0, 1, 2 and 3 kGy) were used to evaluate the stability and thermal degradation kinetics of anthocyanins, as well as the stability of total phenolic compounds (TPC) and antioxidant activity at different temperatures (4, 25, 35 and 45 °C) during the storage (0, 30, 60, 90 and 120 days) of black rice flour. This flour can be used as ingredient for gluten-free cereal products with higher nutritional value. For this it is necessary to preserve the anthocyanin content during thermal processing and shelf-life periods. At time 0, the dose of 3 kGy provided all of the most available bioactive compounds, raising their antioxidant potential, except for TPC. During the storage at different temperatures up to 120 days, gradual... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthocyanins; Bioactive compounds; Irradiation; Stability; Thermodynamic parameters; Pigmented rice. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100500 |
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Kvitschal,Marcus Vinícius; Vidigal Filho,Pedro Soares; Scapim,Carlos Alberto; Gonçalves-Vidigal,Maria Celeste; Sagrilo,Edvaldo; Pequeno,Manoel Genildo; Rimoldi,Fabrício. |
The objective of this work was to compare different phenotypic stability methods by using yield and storage root dry matter content data of eight cassava genotypes, assessed in eight environments in northwest of Paraná State, Brazil. All the methodologies applied showed to be able to study the stability of cassava genotypes, but each with its peculiarities. The methodologies of Eskridge, Annicchiarico and Lin and Binns were the most appropriated on situation with smaller effect of G x E interaction. The AMMI analysis and the Toler and Burrows methodology were the most specific on detailing specific adaptations of cassava genotypes to favorable and unfavorable environments. It could be suggested to use simultaneous AMMI analysis and Toler and Burrows... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Correlation; Stability; Safety first indexes; Non-linear regression; AMMI analysis. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100022 |
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Chen,Meng-Yan; Zheng,Wei; Dong,Qiu-Yue; Li,Zhen-Hua; Shi,Lu-E; Tang,Zhen-Xing. |
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Encapsulation; Alginate; Whey protein; Probiotics; Stability. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736 |
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Gonçalves,Paulo de Souza; Moraes,Mário Luiz Teixeira de; Silva,Marcelo de Almeida; Gouvêa,Lígia Regina Lima; Aguiar,Adriano Tosoni da Eira; Costa,Reginaldo Brito da. |
Twenty two open-pollinated Hevea progenies from different parental clones of the Asian origin were tested at five sites in the Northwestern São Paulo State Brazil to investigate the progeny girth growth, rubber yield, bark thickness and plant height. Except for the rubber yield, the analysis of variance indicated highly significant (p<0.01) genotype x environment interaction and heterogeneity of regressions among the progenies. However, the regression stability analysis identified only a few interacting progenies which had regression coefficients significantly different from the expected value of one. The linear regressions of the progeny mean performance at each test on an environmental index (mean of all the progenies in each test) showed the general... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hevea brasiliensis; Stability; Adaptability; Environmental index; Linear regression. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100004 |
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Verdolin,B.A.; Ficker,S.M.; Faria,A.M.C.; Vaz,N.M.; Carvalho,C.R.. |
Initial contacts with a T-dependent antigen by mucosal routes may result in oral tolerance, defined as the inhibition of specific antibody formation after subsequent parenteral immunizations with the same antigen. We describe here an additional and permanent consequence of these initial contacts, namely, the blockade of secondary-type responsiveness to subsequent parenteral contacts with the antigen. When repeatedly boosted ip with small doses (3 µg) of ovalbumin (OVA) (or lysozyme), primed B6D2F1 mice showed progressively higher antibody responses. In contrast, mice primed after a single oral exposure to the antigen, although repeatedly boosted, maintained their secondary antibody titers on a level which was inversely proportional to the dose of antigen... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oral tolerance; Mucosal immunity; Stability; Memory. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200008 |
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Lu,Y.; Rong,C.Z.; Zhao,J.Y.; Lao,X.J.; Xie,L.; Li,S.; Qin,X.. |
The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Storage time; Chlamydia trachomatis; Ureaplasma urealyticum; Neisseria gonorrhoeae; Stability; QRT-PCR. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000701 |
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França,C.N.; Mendes,C.C.; Ferreira,C.E.S.. |
The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipid profile; Storage; Temperature; Stability; Refrigerator; Room temperature. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300608 |
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Jiang,Cheng; Song,Jinzhu; Zhang,Junzheng; Yang,Qian. |
Abstract Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34 mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value. The optimum process conditions were found to be acetone as the extractant, under room temperature, and at a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaetoglobosin A; Cornstalk; Purification; Stability; Chaetomium globosum. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300410 |
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Mercurio,Daiane Garcia; Calixto,Lívia Salomão; Campos,Patrícia Maria Berardo Gonçalves Maia. |
Considering the importance of an adequate composition of the formulation in the development of stable, safe and effective cosmetic products, experimental design techniques are tools that can optimize the formulation development process. The objective of this study was to develop topical formulations using the Box-Behnken design with response surface methodology and evaluate its physical, sensory and moisturizing properties. The experimental design used in the first step allowed to identify and to quantify the influence of raw materials, as well as the interaction between them. In the second step, the analysis identified the influence of soy lecithin, the phytantriol and capric acid triglyceride and caprylic on the consistency index, stickiness and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Emulsions; Formulation; Stability; Skin barrier; Experimental design. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100624 |
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Arantes,Paula de Oliveira; Santos,Quesia Nery dos; Freitas,Zaida Maria Faria de; Pyrrho,Alexandre dos Santos; Cerqueira-Coutinho,Cristal; Villa,Ana Lucia Vazquez; Santos,Elisabete Pereira dos; Ricci-Júnior,Eduardo. |
ABSTRACT This article reports the development and characterization of a nanoemulsion (NE) able to improve the cutaneous penetration of nifedipine. NE with nifedipine was development and characterized, presenting droplet size of 20 nm with low polydispersity index (IP<0.1), spherical shape without aggregation, pH compatible with typical skin levels and stability evaluated by seven months. In the permeation studies, a classical formulation based in an oil/water cream containing nifedipine was used for comparison with NE. Nanoemulsion promoted and improved the retention of nifedipine in the epidermis and dermis in relation to classical formulation. This promoting effect is related to the nanometric size of the droplets of the NE (20 nm), which give him a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Topical nifedipine/cutaneous permeation; Nanoemulsion/characterization; Stability; Cutaneous permeation/study; Healing of wounds. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200628 |
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Castilhos,Juliana Kich; Dillenburg,Thaís Luise; Antunes,Marina Venzon; Scribel,Letícia; Zavascki,Alexandre Prehn; Linden,Rafael; Verza,Simone Gasparin. |
Polymyxins are a group of antibacterial substances and have remained the drugs of choice for treatment of resistant Gram-negative bacilli. Polymyxin B is administered by intravenous infusion and requires the reconstitution of lyophilized powder with 0.9% saline or 5% glucose solutions. To date, there is little information about polymyxin stability in different infusions solutions, especially at 40 ºC, a temperature that is recommended to study drug stability as it accelerates degradation reactions. Therefore, in this work an analytical method using LC-MS/MS was developed, validated and applied to determine the stability of polymyxin B diluted in 0.9% saline or 5% glucose solutions at 25 ºC and 40 ºC. The stability of polymyxin B solutions was evaluated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymyxin; Stability; LC-MS/MS; Glucose solutions; Saline solutions. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100537 |
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Lima,Isabela Angeli de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara. |
ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nanoparticles; Ferulic acid/encapsulation efficiency; Ferulic acid/quantification; Stability; High performance liquid chromatography/validation; PLGA-poly(lactide-co-glycolide)/nanoparticles; Chitosan.. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200627 |
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Registros recuperados: 90 | |
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