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| Registros recuperados: 992 | |
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| CALÁBRIA,LUCIANA KAREN; TEIXEIRA,RENATA ROLAND; COELHO GONÇALVES,SYBELLI MAGDA; PASSOS LIMA,ANDREIA BARCELOS; SANTOS,ANA ALICE DINIZ DOS; MARTINS,ANTÔNIO ROBERTO; ESPINDOLA,FOUED SALMEN. |
| The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain. |
| Tipo: Journal article |
Palavras-chave: Antigen retrieval; Microwave oven; Steam cooker. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002 |
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| Rabelo-Gonçalves,Elizabeth M A; Nishimura,Nancy F; Murilo R Zeitune,José. |
| An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori... |
| Tipo: Journal article |
Palavras-chave: BALB/c mouse; Experimental model; Helicobacter pylori. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012 |
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| DEMURO,ANGELO; PARKER,IAN. |
| The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes |
| Tipo: Journal article |
Palavras-chave: Single-channel recording; Calcium imaging; TIRFM; N-type Ca2+ channels. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025 |
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| Palma,Verónica; Carrasco,Héctor; Reinchisi,Gisela; Olivares,Gonzalo; Faunes,Fernando; Larraín,Juan. |
| Proliferation and cell fate determination in the developing embryo are extrinsically regulated by multiple interactions among diverse secreted factors, such as Sonic Hedgehog (SHh), which act in a concentration-dependent manner. The fact that SHh is secreted as a lipid-modified protein suggests the existence of a mechanism to regulate its movement across embryonic fields. We have previously shown that heparan sulfate proteoglycans (HSPGs) are required for SHh binding and signalling. However, it was not determined which specific HSPG was responsible for these functions. Here we evaluated the contribution of perlecan on SHh localization and activity. To understand the mechanism of action of perlecan at the cellular level, we studied the role of perlecan-SHh... |
| Tipo: Journal article |
Palavras-chave: SHh; Morphogen gradients; Perlecan. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100008 |
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| Danciu,Corina; Vlaia,Lavinia; Fetea,Florinela; Hancianu,Monica; Coricovac,Dorina E; Ciurlea,Sorina A; M Şoica,Codruţa; Marincu,Iosif; Vlaia,Vicentiu; Dehelean,Cristina A; Trandafirescu,Cristina. |
| BACKGROUND: Curcuma longa Linnaeus and Zingiber officinale Roscoe are two main representatives ofZingiberaceae family studied for a wide range of therapeutic properties, including: antioxidant, anti-inflammatory, anti-angiogenic, antibacterial, analgesic, immunomodulatory, proapoptotic, anti-human immunodeficiency virus properties and anticancer effects. This study was aimed to analyse the ethanolic extracts of Curcuma rhizome (Curcuma longa Linnaeus) and Zingiber rhizome (Zingiber officinale Roscoe) in terms of polyphenols, antioxidant activity and anti-melanoma potential employing the B164A5 murine melanoma cell line. RESULTS: In order to evaluate the total content of polyphenols we used Folin-Ciocâlteu method. The antioxidant activity of the two... |
| Tipo: Journal article |
Palavras-chave: Curcuma longa Linnaeus; Zingiber officinale Roscoe; Polyphenols; Antioxidant; Melanoma. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100001 |
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| Nikolic,Ljiljana M; Rokic,Milos B; Todorovic,Natasa V; Kartelija,Gordana S; Nedeljkovic,Miodrag S; Zakrzewska,Joanna S. |
| The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by 31P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na+/K+-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and... |
| Tipo: Journal article |
Palavras-chave: ELF-MF; Garden snail; 31P NMR spectroscopy; Phosphate turnover enzymes. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200012 |
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| Cetin,Emine Sema; Babalik,Zehra; Hallac-Turk,Filiz; Gokturk-Baydar,Nilgun. |
| BACKGROUND: Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell... |
| Tipo: Journal article |
Palavras-chave: Cadmium chloride; Cell; Secondary metabolite. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100047 |
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| COURT,FELIPE A; ÁLVAREZ,JAIME. |
| In this essay, we show that several anatomical features of the axon, namely, microtubular content, caliber and extension of sprouts, correlate on a local basis with the particular condition of the glial cell, i.e., the anatomy of axons is dynamic, although it is seen usually in its `normal' state. The occurrence of ribosomes and messenger RNAs in the axon suggests that axoplasmic proteins are most likely synthesized locally, at variance with the accepted notion that they are supplied by the cell body. We propose that the supporting cell (oligodendrocyte or Schwann cell) regulates the axonal phenotype by fine-tuning the ongoing axonal protein synthesis. |
| Tipo: Journal article |
Palavras-chave: Axon; Protein synthesis; Nerve regeneration; Schwann cell; Wld s. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400009 |
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| OCAMPO-GARCÉS,ADRIÁN; MENA,WILSON; HERNÁNDEZ,FELIPE; CORTÉS,NELSON; PALACIOS,ADRIÁN G. |
| Rest activity pattern was studied in wild-captured males of Octodon degus (n=9), Octodon bridgesi (n=3), and Spalacopus cyanus (n=6) (Rodentia: Octodontidae). Ten-minute resolution actograms were constructed from data obtained by an automated acquisition system. After two months of habituation to a stable light-dark schedule, recordings were performed in isolation chambers under a 12: 12 Light Dark schedule. A free-running period (constant darkness) was recorded for O. bridgesi and S. cyanus. O. degus displayed a crepuscular pattern of rest activity rhythm. Entrained O. bridgesi and S. cyanus displayed nocturnal preference, with rest anticipating light phase and without crepuscular activity bouts. Under constant darkness, active phase occurred at... |
| Tipo: Journal article |
Palavras-chave: Circadian rhythms; Chronotype; Octodon; Octodontids; Spalacopus cyanus. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200003 |
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| Pino-Lagos,Karina; Michea,Paula; Sauma,Daniela; Alba,Andrea; Morales,Jorge; Bono,María Rosa; Fierro,Alberto; Rosemblatt,Mario. |
| One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and... |
| Tipo: Journal article |
Palavras-chave: CD4+CD25+ regulatory T cell; Cyclosporine A; Dendritic cells; Transplant tolerance. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010 |
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| Zheng,Huan; Xu,Huifeng; Cui,Bin; Xie,Nanzi; Wang,Zhi; Luo,Ming. |
| Purpose: The G-protein β3-subunit gene C825T polymorphism (GNB3-C825T) has been reported to be associated with essential hypertension (EH), but results from previous studies are conflicting. The present study aimed at investigating the association between this polymorphism and risk of EH using a meta-analysis on the published studies. Materials and Methods: PubMed, Embase, CBM (China Biological Medicine Database), Wanfang and VIP databases were searched to identify eligible studies published in English and Chinese before March 2013. Data were extracted using standardized methods. The association was assessed by the odds ratio (OR) with 95% confidence intervals (CI). Begg's test was used to measure publication bias. Results: A total of 40... |
| Tipo: Journal article |
Palavras-chave: GTP-Binding Proteins; Polymorphism; Genetic hypertension; Meta-Analysis. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300007 |
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| Registros recuperados: 992 | |
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