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| Registros recuperados: 978 | |
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| Li,Biaoru. |
| Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is... |
| Tipo: Journal article |
Palavras-chave: Genome; Genomic expression; Genomic expression analysis at single cell; Single cell. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100011 |
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| Li,Liang-Mei; Li,Jun; Zhang,Xiu-Ying. |
| Background: The antimicrobial properties and molecular interaction analysis of curcumin and its derivatives against three different strains of Streptococcus pneumoniae (Penicillin-susceptible, Penicillin-intermediate and Penicillin-resistant) are studied. Results: These properties were analyzed based on the measurement of the inhibition zone, minimum inhibitory concentration (MIC), and rate of kill revealed that curcumin monoglucoside, curcumin diglucoside and curcumin possessed strong antimicrobial properties even on the Penicillin-resistant strains. Additionally, the molecular docking simulation analyses against Penicillin Binding Protein of S. pneumoniae also confirm that these compounds docked at the active site of the enzyme. Further, the molecular... |
| Tipo: Journal article |
Palavras-chave: Antimicrobial; Curcumin; MIC; Molecular docking; Penicillin-resistant. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100002 |
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| Ren,Xueying; Zhang,Xuecheng; Sui,Zhenghong. |
| Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples,... |
| Tipo: Journal article |
Palavras-chave: Female gametophyte; Gracilaria/Gracilariopsis lemaneiformis; Suppression subtractive hybridization; Tetrasporophyte; Virtual Northern blot. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006 |
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| Zepeda-Batista,José Luis; Alarcón-Zúñiga,Baldomero; Ruíz-Flores,Agustín; Núñez-Domínguez,Rafael; Ramírez-Valverde,Rodolfo. |
| The objective was to estimate the allelic and genotypic frequencies, genetic diversity and polymorphic information content for the β-casein, κ-casein and β-lactoglobulin genes. Blood and frozen semen samples were collected from 453 Jersey individuals registered by the Mexican Jersey Cattle Association. Twenty eight breed specific SNP primers for whole genes were used. The B allele of κ-casein had higher frequency (0.69) than the A (0.26) and E (0.05). For β-lactoglobulin, the highest frequency was for B (0.72), followed by A and C alleles (0.26 and 0.02, respectively). The β-casein allele with the highest frequency was A² (0.71), followed by A¹ (0.19), A³ (0.05), B... |
| Tipo: Journal article |
Palavras-chave: β -Casein β -Lactoglobulin Genetic diversity κ -Casein. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100001 |
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| Tang Wang,Chuan; Dao Yang,Xin; Xu Chen,Dian; Lin Yu,Shan; Zhen Liu,Guang; Yi Tang,Yue; Zhi Xu,Jian. |
| SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts |
| Tipo: Journal article |
Palavras-chave: Groundnut; Isolation; Simple sequence repeat. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015 |
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| Soto,Carmen. |
| Background: Lactobacillus sp. are probiotic microorganisms, and some of them are able to produce conjugated linoleic acid (CLA) via the bio-hydrogenation of linoleic acid (LA). Both CLA and LA are polyunsaturated fatty acids commonly used in the prevention and control of cardiovascular disease, high cholesterol, and cancer, among other ailments. The carbon source is one variable that can affect the growth and characteristics of these bacteria. Molecules called prebiotics are known to benefit human health by stimulating the growth and activity of probiotic bacteria present in the intestinal microflora. The aim of this study was to evaluate how different oligosaccharides affect the growth and fatty acid profile of Lactobacillus plantarum (NRRL - B4496). L.... |
| Tipo: Journal article |
Palavras-chave: CLA; Fatty acid profile; Lactobacillus plantarum; Oligosaccharides. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400008 |
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| Njung'e,Vincent; Deshpande,Santosh; Siambi,Moses; Jones,Richard; Silim,Said; De Villiers,Santie. |
| Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles... |
| Tipo: Journal article |
Palavras-chave: Genetic variation; Genotyping; Microsatellites; Seed purity. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008 |
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| Sequeida,Álvaro; Tapia,Eduardo; Ortega,Marcelo; Zamora,Pablo; Castro,Álvaro; Montes,Christian; Zúñiga,Gustavo E; Prieto,Humberto. |
| Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity... |
| Tipo: Journal article |
Palavras-chave: Deschampsia antarctica; Phenolic compounds; Temporary immersion bioreactor; UV-B elicitation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008 |
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| Yoon,Min-Young; Moe,Kyaw Thu; Kim,Dae-Young; Rho,Il-Rae; Kim,Su; Kim,Ki-Taek; Won,Mi-Kyoung; Chung,Jong-Wook; Park,Yong-Jin. |
| In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a... |
| Tipo: Journal article |
Palavras-chave: Genetic diversity; Population structure; SSR markers; Strawberry. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200006 |
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| Stove,Veronique; Smits,Kaatje; Naessens,Evelien; Plum,Jean; Verhasselt,Bruno. |
| RNA interference (RNAi), mediated by short double-stranded RNAs, is a powerful mechanism for posttranscriptional gene silencing. Sustained expression of short hairpin RNA (shRNA) can be accomplished in mammalian cells by viral delivery systems. Using lentiviral constructs, stable gene silencing is established both in dividing and non-dividing cells. Targeting one single gene can lead to the development of escape mutants or may be insufficient to silence redundant pathways. Therefore, simultaneous targeting of multiple genes may be necessary. We have generated a lentiviral vector-based system for expression of multiple shRNAs from a single viral vector, which also encodes an EGFP reporter protein. We show that knock-down of each single gene from multiple... |
| Tipo: Journal article |
Palavras-chave: EGFP; Lentiviral gene transfer; Multiple knock-down; RNAi; Rho GTPases; ShRNA. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500013 |
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| Nematollahia,Amene; Sohrabvandi,Sara; Mortazavian,Amir Mohammad; Jazaeri,Sahar. |
| Background: Increased popularity of vegetarianism, lactose intolerance, and the high cholesterol content in dairy products, are all factors that have recently increased the demand for nondairy probiotic products. The objective of this study is to evaluate the effect of refrigeration on the viability of probiotics and asses some of the chemical and sensory characteristics in cornelian cherry juice. Results: The Iranian native probiotic strain (L. casei T4) showed greater viability compared to industrial types (viable count of 8.67 log cfu/mL versus <6.0 log cfu/mL at d 28). However, this most tolerant Iranian strain, could not withstand the conditions of 'Natural juice' at pH 2.6 for more than 7 d. Following a pH adjusted treatment (to pH ~3.5), the... |
| Tipo: Journal article |
Palavras-chave: Antioxidant activity; Anthocyanin; Cornelian cherry; Phenolic compounds; Probiotic; Viability. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300005 |
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| Wankasi,Donbebe; Horsfall Jnr,Michael; Ibuteme Spiff,Ayabaemi. |
| The sorption kinetics of Pb2+ and Cu2+ ions in aqueous solution by unmodified and mercaptoacetic acid modified biomass of Nypa fruticans shoot has been investigated. The equilibrium sorption capacity of Pb2+ and Cu2+ was determined from the Langmuir equation and found to be 15.59 mg/g and 21.85 mg/g for unmodified biomass and 52.86 mg/g and 66.71 mg/g for modified biomass at 30ºC. The time dependent studies showed relatively rapid sorption of 5 - 10 min to reach equilibrium for both metal ions. A batch sorption model, based on the assumption of the pseudo-second-order mechanism, was applied to predict the rate constants (g mg-1 min-1), which were found to be 3.59 x 10-4 (Pb2+) and 1.85 x 10-4 (Cu2+) and 2.82 x 10-2 (Pb2+) and 3.67 x 10-2 (Cu2+) on... |
| Tipo: Journal article |
Palavras-chave: Adsorption; Heavy metal binding; Nipah palm. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500015 |
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| Registros recuperados: 978 | |
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