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| Registros recuperados: 978 | |
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| Xu,Ji-Fei; Mi,Yuan-Ting; Ren,Nan-Qi. |
| The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH.... |
| Tipo: Journal article |
Palavras-chave: Acetate; Biohydrogen; Buffering action; Ethanoligenens harbinense phosphate. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002 |
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| LeBlanc-Straceski,Janine; Sobrado,Pablo; Betz,Sharon; Zerfas,Julie; Morgan,Karen. |
| A PCR based strategy was developed to screen a Xenopus oocyte λgt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, which are used as template for PCR. The integrity of the plaques on the low-density plates is preserved. Once a positive LP has been identified,... |
| Tipo: Journal article |
Palavras-chave: CDNA; Lambda; Non-radioactive; PCR; Plaque; Screening. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012 |
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| Zhanga,Yong; Min,Qingsong; Xu,Jian; Zhang,Ke; Chen,Shulin; Wang,Haijun; Li,Demao. |
| Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specificcell growth rate(μ), specific glucose consumption rate (qGlu)and DHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7 g/L, giving a yield of 0.103 g DHA/g... |
| Tipo: Journal article |
Palavras-chave: DHA; Malate; NADPH; Schizochytrium sp. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100009 |
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| Martínez,Claudio; García,Verónica; González,David; Jara,Matías; Aguilera,Matías; Ganga,Maria Angélica. |
| Background: Wine fermentation is a dynamic process and yeast has a precise genic regulation system that allow them to coordinate the gene transcription levels. However, the fermentation stage at which there is a great variation in the genic expression associated to a particular enological trait is often unknown and there are no simple experimental approaches to define it. Results: To identify the most adequate stage in which to evaluate the expression of the genes associated to specific enological traits we identified three stages of fermentation using the industrial strain EC1118. These stages, called early (0-16.4 hrs), middle (16.4-88.7 hrs) and late (88.7 hrs onwards), were characterized according to changes in the speed of CO2 production. The greatest... |
| Tipo: Journal article |
Palavras-chave: Enological traits; Gene expression; Saccharomyces cerevisiae; Wine fermentation; Wine yeast. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400013 |
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| He,Yang; Hou,Pei; Fan,Gang; Li,Dongmei; Peng,Cheng. |
| Background Berberine acts primarily as an important active ingredient in Coptis chinensis and has been traditionally applied in clinical treatment. Nevertheless, little information has been released about C. chinensis, as far as functional genes and biosynthetic pathway of berberine are concerned. Here, we isolated a novel (S)-canadine synthase gene (designated as CAS-1) from C. chinensis by using RT-PCR and RACE techniques. Results Bioinformatics analysis showed that the cDNA is 1942 bp in length with a complete open reading frame (ORF) of 1476 bp, and the ORF encodes a polypeptide of 491 amino acids with a calculated molecular mass of 55.29 kDa and a pI value of 8.92. Real-time quantitative PCR analysis showed that CcCAS-1 was constitutively expressed in... |
| Tipo: Journal article |
Palavras-chave: Berberine biosynthesis; Coptis chinensis; Expression pattern. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500009 |
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| Pacheco Contreras,Víctor I; Lourenco Jaramillo,Diana L; Parra Bracamonte,Gaspar M; Martínez González,Juan C; Sifuentes Rincón,Ana M. |
| K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-casein variants. A strategy based on PCR-RFLP was designed to characterize nine K-casein variants (A, B, C, E, F², G, H, I and J) and used to genotype three cattle populations: Gyrholando (3/8 Gyr x 5/8 Holstein), Charolais and Carora. The B variant was the most frequent in the Charolais and Carora breeds, with allelic frequencies of 0.60 and 0.59, respectively. In the Gyrholando breed, four variants were found, with the A variant being the most frequent and E and... |
| Tipo: Journal article |
Palavras-chave: Milk proteins; Milk quality; Protein genes. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400012 |
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| Diarra,Sabake T; He,Ji; Wang,Jianbo; Li,Jiaru. |
| Background: The perennial medicinal herb Dioscorea zingiberensis is a very important plant used for steroid drug manufacturing for its high level of diosgenin in rhizome. Although the stimulation of diosgenin accumulation by ethylene has been reported in a few of plant species, its regulation is not yet characterized at the molecular level, the underlying molecular mechanism remains elusive. Results: In this study, the effects of ethylene on diosgenin biosynthesis in in vitro cultures of D. zingiberensis were described. The results showed that, in samples treated with ethylene at concentration E3 (10(4) dilution of 40% ethephon), the diosgenin biosynthesis was significantly promoted in comparison with the control samples. Treatment with high concentrations... |
| Tipo: Journal article |
Palavras-chave: Diosgenin; Dioscorea zingiberensis; Ethylene; Gene expression. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500006 |
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| Jain,Ashok K.; Basha,Sheikh Mehboob; Holbrook,C. Corley. |
| We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to identify cDNAs corresponding to transcripts affected by water stress in peanuts (Arachis hypogaea L.). Using this method, we have identified several mRNA transcripts that are up- or down-regulated following water stress. With 21 primer combinations, a total of 1235 differential display products were observed in irrigated samples, compared to 950 differential display products in stressed samples. These products demonstrated qualitative and quantitative differences in the gene expression. The differentially expressed transcripts were collectively named PTRD (Peanut Transcripts Responsive to Drought). We have identified a total of 43 PTRD, which were... |
| Tipo: Journal article |
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| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200005 |
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| Schuler,Ingrid; Orozco,Luis Antonio. |
| The international scenario for biotechnology shows a rapid tendency at industrialized countries in the increase of publications, patents, enterprises and novel solutions for the industry, the environment, health and agriculture. Nevertheless, Colombia has an important delay in relation to the international scientific development and the capacity to generate wealth and services for its productive systems. This delay has been one of the concerns of the country's policy during the last years, and more precisely since 2002, when for the first time biotechnology was included in a National Development Plan as one of the mechanisms for competitiveness and the use of biodiversity and genetic resources. This paper is the result of a survey conducted in 2005 aimed... |
| Tipo: Journal article |
Palavras-chave: Agricultural biotechnology; Indicators; Legislation; Policies; Public perception.. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300001 |
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| Ahmed,Zakaria. |
| Biochemical methods, usually microbial or enzymatic, are suitable for the production of unnatural or rare monosaccharides. D-Arabitol was produced from D-glucose by fermentation with Candida famata R28. D-xylulose can also be produced from D-arabitol using Acetobacter aceti IFO 3281 and D-lyxose was produced enzymatically from D-xylulose using L-ribose isomerase (L-RI). Ribitol was oxidized to L-ribulose by microbial bioconversion with Acetobacter aceti IFO 3281; L-ribulose was epimerized to L-xylulose by the enzyme D-tagatose 3-epimerase and L-lyxose was produced by isomerization of L-RI. L-ribose and L-arabinose were prepared biochemically from ribitol by oxidation using Acetobacter aceti IFO 3281 and isomerization using L-RI and L-arabinose isomerase... |
| Tipo: Journal article |
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| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200008 |
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| Registros recuperados: 978 | |
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