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| Registros recuperados: 992 | |
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| LODATO,P; ALCAÍNO,J; BARAHONA,S; NIKLITSCHEK,M; CARMONA,M; WOZNIAK,A; BAEZA,M; JIMÉNEZ,A; CIFUENTES,V. |
| In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at... |
| Tipo: Journal article |
Palavras-chave: Xanthophyllomyces dendrorhous; Carotenogenesis genes; MRNA expression. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008 |
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| Kargupta,Roli; Puttaswamy,Sachidevi; Lee,Aiden J.; Butler,Timothy E.; Li,Zhongyu; Chakraborty,Sounak; Sengupta,Shramik. |
| Abstract Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection” (TTD). Hence, a method that retains the reliability and low-cost of the MGIT system, while reducing TTD would be highly desirable. Methods: Living bacterial cells possess a membrane potential, on account of which they store charge when subjected to an AC-field. This charge storage (bulk capacitance) can be estimated using impedance measurements at multiple frequencies. An increase in the number of living cells during culture... |
| Tipo: Journal article |
Palavras-chave: Mycobacteria; Tuberculosis; Electrical impedance spectroscopy; BD Bactec MGIT 960; M. bovis BCG; M. smegmatis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100214 |
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| Ruz,Gonzalo A; Timmermann,Tania; Barrera,Javiera; Goles,Eric. |
| BACKGROUND: Interactions between genes and their products give rise to complex circuits known as gene regulatory networks (GRN) that enable cells to process information and respond to external stimuli. Several important processes for life, depend of an accurate and context-specific regulation of gene expression, such as the cell cycle, which can be analyzed through its GRN, where deregulation can lead to cancer in animals or a directed regulation could be applied for biotechnological processes using yeast. An approach to study the robustness of GRN is through the neutral space. In this paper, we explore the neutral space of a Schizosaccharomyces pombe (fission yeast) cell cycle network through an evolution strategy to generate a neutral graph, composed of... |
| Tipo: Journal article |
Palavras-chave: Neutral graph; Boolean networks; Evolution strategy; Fission yeast cell cycle; Attractors. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100058 |
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| Pérez-Ibave,Diana Cristina; González-Alvarez,Rafael; Martinez-Fierro,Margarita de La Luz; Ruiz-Ayma,Gabriel; Luna-Munoz,Maricela; Martínez-De-Villarreal,Laura Elia; Garza-Rodríguez,María De Lourdes; Reséndez-Pérez,Diana; Mohamed-Noriega,Jibran; Garza-Guajardo,Raquel; Bautista-De-Lucío,Victor Manuel; Mohamed-Noriega,Karim; Barboza-Quintana,Oralia; Arámburo-De-La-Hoz,Carlos; Barrera-Saldana,Hugo Alberto; Rodríguez-Sánchez,Irám Pablo. |
| Tipo: Journal article |
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| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100044 |
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| Silva,Doris; Lizama,Carlos; Tapia,Verónica; Moreno,Ricardo D. |
| Mammalian germ cell apoptosis plays a key role in controlling the correct number of germ cells supported by Sertoli cells during the first wave of spermatogenesis in mammalian puberty. However, little is known about hormonal factors that could influence the rate of germ cell apoptosis during puberty or adulthood. In this work we evaluate germ cell apoptosis under hypothyroidism induced by goitrogen propylthiouracil (PTU) during the first wave of spermatogenesis. Neonatally administered PTU promoted a delay in the differentiation of Sertoli cells as evaluated by the expression of clusterin using immunohistochemistry and RT-PCR. Clusterin had different expression levels in control and PTU-treated animals, but under both conditions the highest levels were... |
| Tipo: Journal article |
Palavras-chave: Testis; Spermatocyte; Sertoli cell; Caspase; Apoptosis; Thyroid. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200010 |
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| Leonova,Olga G; Karajan,Bella P; Ivlev,Yuri F; Ivanova,Julia L; Skarlato,Sergei O; Popenko,Vladimir I. |
| We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved... |
| Tipo: Journal article |
Palavras-chave: 3D reconstruction; Ciliates; Electron microscopy; Macronucleus; Nucleoli. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010 |
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| Quiroz,Karla A.; Berrios,Miguel; Carrasco,Basilio; Retamales,Jorge B.; Caligari,Peter D. S.; García-Gonzáles,Rolando. |
| Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a... |
| Tipo: Journal article |
Palavras-chave: Fragaria chiloensis; Meristem culture; Plant growth regulators; Virus elimination; Plant morphogenesis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404 |
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| Hwan-Hee,Jang; Su-Yeon,Cho; Mi-Ju,Kim; Jung-Bong,Kim; Sung-Hyen,Lee; Mee-Young,Lee; Young-Min,Lee. |
| BACKGROUND: Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. Here, the effects of 80 % ethanol extracts of Salvia plebeia R. Br. (SE) on an induced inflammatory response were investigated RESULTS: Salvia plebeia R. Br. inhibited production of pro-inflammatory cytokines, such as TNF-α and IL-6, as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. NO and pro-inflammatory cytokine production was suppressed more effectively by SE of the aerial parts (SE-A) than of the roots (SE-R) of S. plebeia. In BEAS-2B cells, both SE-A and SE-R inhibited the increase in production of the inflammatory cytokines IL-6 and IL-8. We also investigated the antiasthmatic effects of SE in an... |
| Tipo: Journal article |
Palavras-chave: Anti-inflammatory; Asthma; Salvia plebeia. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100041 |
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| Águila,Luis; Ruedlinger,Jenny; Mansilla,Karina; Ordenes,José; Salvatici,Raúl; Campos,Rui Ribeiro de; Romero,Fernando. |
| BACKGROUND: Ruta graveolens L. (R. graveolens) is a medicinal plant employed in non-traditional medicines that has various therapeutic properties, including anthelmintic, and vasodilatory actions, among others. We evaluated the trachea-relaxant effects of hydroalcoholic extract of R. graveolens against potassium chloride (KCl)- and carbachol-induced contraction of rat tracheal rings in an isolated organ bath. RESULTS: The results showed that the airway smooth muscle contraction induced by the depolarizing agent (KCl) and cholinergic agonist (carbachol) was markedly reduced by R. graveolens in a concentration-dependent manner, with maximum values of 109 ± 7.9 % and 118 ± 2.6 %, respectively (changes in tension expressed as positive percentages of change in... |
| Tipo: Journal article |
Palavras-chave: Ruta graveolens; Trachea-relaxant; Plant extract. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100028 |
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| Vogel,Edgar H. |
| The purpose of this study was to examine whether the progressive disappearance of short-latency conditioned responses, or inhibition of delay, observed in Pavlovian conditioning with long inter-stimulus intervals, could be reverted by the presentation of a novel stimulus. In one experiment, two groups of rabbits received extensive training with a short (250 ms) or a long (1500 ms) tone that overlapped and terminated with a periorbital shock unconditioned stimulus. After training, the presentation of an extraneous stimulus prior to tone onset produced a reinstatement of short latency CRs in the group trained with the long CS, but did not affect CR latency in the group trained with the short CS. This finding is consistent with Pavlov's (1927) view that... |
| Tipo: Journal article |
Palavras-chave: Inhibition of delay; Conditioning; Temporal discrimination; Eyeblink conditioning; Timing. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100008 |
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| DELMAS,PATRICK. |
| Mutations in either polycystin-2 (PC2) or polycystin-1 (PC1) proteins cause severe, potentially lethal, kidney disorders (autosomal dominant polycystic kidney disease, ADPKD) and multiple extrarenal disease phenotypes. PC2, a member of the transient receptor potential channel superfamily and PC1, an orphan membrane receptor of largely unknown function, are thought to be part of a common signalling pathway. Here, I show that co-assembly of full-length PC1 with PC2 forms an ion channel signalling complex in which PC1 regulates PC2 channel gating through a structural rearrangement of the polycystin complex (Delmas et al., 2004a). These polycystin complexes function either as a receptor-cation channel or as a G-protein-coupled receptor. Thus, PC1 acts as a... |
| Tipo: Journal article |
Palavras-chave: Polycystin; TRP channel; Sensory transduction; Calcium signaling; Polycystic Kidney Disease. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400026 |
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| Registros recuperados: 992 | |
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