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Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial and antitumor activities BJM
Cao,Xiao-Hong; Liao,Zhen-Yu; Wang,Chun-Ling; Yang,Wen-Yan; Lu,Mei-Fang.
A lipopeptide biosurfactant produced by Bacillus natto TK-1 has a strong surface activity. The biosurfactant was found to be an anti-adhesive agent against several bacterial strains, and also showed a broad spectrum of antimicrobial activity. The biosurfactant induced a significant reduction in tumor cells viability in a dose- dependent manner.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Anti-adhesive; Antimicrobial activity; Antitumor.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200030
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Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae) BJM
Salvador,Marcos J.; Pereira,Paulo S.; França,Suzelei C.; Candido,Regina C.; Ito,Izabel Y.; Dias,Diones A..
The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternanthera maritima; Adult plants; Callus culture; Biotechnology; Antimicrobial activity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100022
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Antimicrobial activities of six essential oils commonly used as condiments in Brazil against Clostridium perfringens BJM
Radaelli,Marcela; Silva,Bárbara Parraga da; Weidlich,Luciana; Hoehne,Lucélia; Flach,Adriana; Costa,Luiz Antonio Mendonça Alves da; Ethur,Eduardo Miranda.
Abstract Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC–MS (gas chromatography–mass spectrometry). The identities of the isolated compounds...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food-borne disease; Antimicrobial activity; Clostridium perfringens; Spices; Essential oils.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200424
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Antimicrobial activity evaluation and comparison of methods of susceptibility for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter spp. isolates BJM
Rechenchoski,Daniele Zendrini; Dambrozio,Angélica Marim Lopes; Vivan,Ana Carolina Polano; Schuroff,Paulo Alfonso; Burgos,Tatiane das Neves; Pelisson,Marsileni; Perugini,Marcia Regina Eches; Vespero,Eliana Carolina.
Abstract The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism of resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated the activity of antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, and was performed a comparison of the methodologies used to determine the susceptibility: broth microdilution, Etest® (bioMérieux), Vitek 2® automated system (bioMérieux) and disc diffusion. It was calculated the minimum inhibitory concentration (MIC) for each antimicrobial and polymyxin B showed the lowest concentrations for broth microdilution. Errors also were calculated among the techniques, tigecycline and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Enterobacter spp.; KPC; Methods of susceptibility.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300509
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Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum BJM
Quadros,Cedenir Pereira de; Duarte,Marta Cristina Teixeira; Pastore,Gláucia Maria.
In this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from Bacillus subtilis and an alkaline lipase from Fusarium oxysporum (AL/BS mix) on several types of microorganisms, as well as their abilities to remove Listeria innocua ATCC 33093 biofilm from stainless steel coupons. The AL/BS mix had a surface tension of around 30 mN.m-1, indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. The antimicrobial activity of the AL/BS mix was determined by minimum inhibitory concentration (MIC) micro-assays. Among all the tested organisms, the presence of the mixture only affected the growth of B. subtilis CCT 2576, B. cereus ATCC 10876 and L. innocua. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Listeria innocua; Microbial biofilm; Minimum inhibitory concentration; Stainless steel surface.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100045
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Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis BJM
Cândido,Cinthya S.; Portella,Cadmo Silton A.; Laranjeira,Bruno J.; Silva,Sérgio S. da; Arriaga,Angela M.C.; Santiago,Gilvandete M. P.; Gomes,Geovany A.; Almeida,Paulo César; Carvalho,Cibele B. M..
The essential oil from the leaves of Myrcia ovata Cambess., commonly used in Brazil for the treatment of gastric illnesses, was screened for antimicrobial activity and action in the formation of microbial biofilms by Enterococcus faecalis. The oil was obtained by hydrodistillation using a clevenger-type system. Its chemical composition was analyzed using GC and GC-MS. Both MIC and MBC of the essential oil were determined by broth microdilution techniques and agar dilution method. The essential oil showed antimicrobial activity against E. faecalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus, Streptococcus pneumoniae and Candida parapsilosis. The results showed that the essential oil of M. ovata Cambess. was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Myrcia ovata Cambess.; Antimicrobial activity; Biofilms.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300012
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Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae) essential oil BJM
Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira.
Origanum vulgare L. (oregano), Lamiaceae, has been known as plant specie with prominent biological properties for a long time. This study aimed to evaluate the antibacterial activity of Origanum vulgare essential oil on various Gram-positive and Gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food conservation micromodel. The results showed a strong antibacterial activity of the assayed essential oil noted by large growth inhibition zones (30-37 mm). MIC values were between 20-40 µL/mL for the most bacteria strains. Essential oil was able to cause significant (P<0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Origanum vulgare L.; Essential oil; Antimicrobial activity; Bacteria.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400023
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Modulation of antimicrobial metabolites production by the fungus Aspergillus parasiticus BJM
Bracarense,Adriana A.P.; Takahashi,Jacqueline A..
Biosynthesis of active secondary metabolites by fungi occurs as a specific response to the different growing environments. Changes in this environment alter the chemical and biological profiles leading to metabolites diversification and consequently to novel pharmacological applications. In this work, it was studied the influence of three parameters (fermentation length, medium composition and aeration) in the biosyntheses of antimicrobial metabolites by the fungus Aspergillus parasiticus in 10 distinct fermentation periods. Metabolism modulation in two culturing media, CYA and YES was evaluated by a 2² full factorial planning (ANOVA) and on a 2³ factorial planning, role of aeration, medium composition and carbohydrate concentration were also evaluated. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus parasiticus; Factorial planning; Metabolites diversification; Kojic acid; Antimicrobial activity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100045
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Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chresta scapigera BJM
Schinor,Elisandra C.; Salvador,Marcos J.; Ito,Izabel Y.; Dias,Diones A..
Crude extracts and eight isolated compounds from Chresta scapigera were evaluated for antibacterial and antifungal activities by the agar-well diffusion method. Twenty strains, including Gram-positive and Gram-negative bacteria and yeasts were used in the bioassay. Hexane extracts presented the best results while ethanol extracts did not indicate inhibition of the microbial growth. Amongst the evaluated compounds b-amyrin acetate, tiliroside and luteolin showed the strongest antimicrobial effect.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chresta scapigera; Flavonoids; Triterpenes; Antimicrobial activity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100030
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Screening methods to determine antibacterial activity of natural products BJM
Valgas,Cleidson; Souza,Simone Machado de; Smânia,Elza F A; Smânia Jr.,Artur.
The emergence of new infectious diseases, the resurgence of several infections that appeared to have been controlled and the increase in bacterial resistance have created the necessity for studies directed towards the development of new antimicrobials. Considering the failure to acquire new molecules with antimicrobial properties from microorganisms, the optimization for screening methods used for the identification of antimicrobials from other natural sources is of great importance. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar diffusion; Antimicrobial activity; Bioautographic methods; Minimum inhibitory concentration; Minimum bactericidal concentration; Natural products.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200034
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The sensitivity of bacterial foodborne pathogens to Croton blanchetianus Baill essential oil BJM
Melo,Geiseanny Fernandes do Amarante; Costa,Ana Caroliny Vieira da; Garino Junior,Felício; Medeiros,Rosália Severo; Madruga,Marta Suely; Queiroga Neto,Vicente.
The aim of this study was to assess the activity of essential oil extracted from the leaves of C. blanchetianus Baill, popularly known as "marmeleiro", in inhibiting the growth and survival of pathogenic microorganisms in food by determining their survival in vitro and by observing the behaviour of Listeria monocytogenes inoculated into a food model (meat cubes) that was stored at refrigeration temperature (7 ± 1 ºC) for 4 days. The results indicated a bactericidal effect against Aeromonas hydrophila and Listeria monocytogenes and bacteriostatic action against Salmonella Enteritidis. A bacteriostatic effect on meat contaminated with L. monocytogenes was found for all concentrations of essential oils tested. These results showed that essential oil from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Croton blanchetianus Baill; Antimicrobial activity; Foodborne microorganisms.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400023
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Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity BJM
Carlos,Lanamar de Almeida; Amaral,Kenas Aguiar da Silva; Vieira,Ivo José Curcino; Mathias,Leda; Braz-Filho,Raimundo; Samarão,Solange Silva; Vieira-da-Motta,Olney.
Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rauvolfia grandiflora; Staphylococcus aureus; Enterotoxin; Antimicrobial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011
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Probiotic features of two oral Lactobacillus isolates BJM
Zavisic,Gordana; Petricevic,Sasa; Radulovic,Zeljka; Begovic,Jelena; Golic,Natasa; Topisirovic,Ljubisa; Strahinic,Ivana.
In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Antimicrobial activity; Hipolypemic effect.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100050
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Antimicrobial efficacy of secondary metabolites from Glomerella cingulata BJM
Hara Kishore,K.; Misra,Sunil; Ramesh Chandra,D.; Prakash,K.V.V. R.; Suryanarayana Murty,U..
Fungi are known to produce a vast array of secondary metabolites that are gaining importance for their biotechnological applications. Early reports suggest that G. cingulata has the capability to transform many compounds by various enzymatic actions. Therefore, the focus of this study was to determine the antibacterial and antifungal activity of crude ethyl acetate extract of G. cingulata using agar cup bioassay method. Crude extract of G. cingulata exhibited remarkable antifungal activity against Rhizopus oryzae, Chrysoporium tropicum and Beauveria bassiana but no antifungal activity was found against Alternaria tenuissima and Aspergillus niger at any concentrations. The crude extract presented no antibacterial activity against Gram positive and Gram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Glomerella cingulata; Agar cup bioassay.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100031
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Potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of Ilha Do Mel, Paraná, Brazil BJM
Dalitz,Camila de Araújo; Porsani,Mariana Vieira; Figel,Izabel Cristina; Pimentel,Ida C.; Dalzoto,Patrícia R..
Abstract Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacteria; Melanized fungi; Antimicrobial activity; 16S rDNA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100032
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Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity BJPS
Almeida,Tamires Cunha; Seibert,Janaina Brandão; Almeida,Sávio Henrique de Souza; Amparo,Tatiane Roquete; Teixeira,Luiz Fernando de Medeiros; Barichello,Jose Mario; Postacchini,Bruna Bueno; Santos,Orlando David Henrique dos; Silva,Glenda Nicioli da.
Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Cytotoxic activity; F127®; Polymeric micelles; Resveratrol.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100567
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Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) BJPS
Nassima,Boumghar; Nassima,Behidj; Riadh,Ksouri.
The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of Tizi-Ouzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Populus nigra; Populus alba; HPLC; Polyphenols; Antimicrobial activity; Antibiofilm activity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100563
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In vitro antimicrobial activity of total extracts of the leaves of Petiveria alliacea L. (Anamu) BJPS
Ochoa Pacheco,Ania; Marín Morán,Jorge; González Giro,Zenia; Hidalgo Rodríguez,Adrian; Mujawimana,Rachel Juliet; Tamayo González,Karel; Sariego Frómeta,Sandra.
The antimicrobial activity of 13 total extracts was evaluated, 10 soft extracts (B) and 3 blended extracts (E) prepared from dry and fresh leaves of Petiveria alliacea L. Various solvents were used for their preparation: hydroalcoholic solution at 30%, 80% and isopropyl alcohol. The antimicrobial effect of the extracts was tested by means of the method of Kirby-Bauer, using four bacterial strains from the ATCC collection (Escherichia coli, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa) and a leveduriform fungus (Candida albicans). The following quality control parameters were determined for most active extracts: physical, physical-chemical and chemical parameters. The results were: nine extracts showed antibacterial activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Anamu; Soft extracts; Blended extracts; Minimum Inhibitory Concentration.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200006
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Antioxidant, antimicrobial and phytotoxic activities of Rhaponticum acaule DC. essential oil BJPS
Mosbah,Habib; Sassi,Ahlem Ben; Chahdoura,Hassiba; Snoussi,Mejdi; Flamini,Guido; Achour,Lotfi; Selmi,Boulbaba.
The in-vitro antioxidant activity of Rhaponticum acaule essential oil (RaEO) was evaluated using β-carotene/linoleic acid bleaching, chelating activity, and lipid peroxidation inhibition (TBARS) assays. The antimicrobial activity of RaEO was assessed by disc diffusion and microdilution methods against 8 bacteria and 4 yeast. Finally, the allelopathic activity of RaEO on the seed germination and the shoot and root elongation of lettuce (Lactuca sativa L.) seedlings were investigated. According to our results, the RaEO exhibited significant antioxidant activity, similar to those of standards (BHT and ascorbic acid) with IC50 values of 0.042 and 0.045 mg/mL obtained by β-carotene bleaching and TBARS assays, respectively. On the other hand, despite its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhaponticum acaule essential oil; Antioxidant activity; Antimicrobial activity; Allelopathic activity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100611
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Pathogen control using a natural Chilean bee pollen extract of known botanical origin Ciencia e Investigación Agraria
Cabrera,Carol; Montenegro,Gloria.
Bee pollen is a product from beehives that is contained in corbiculae, generally monospecific and is currently considered as a functional food due to its nutritional and medicinal properties. This study investigates the antimicrobial activity of a Chilean bee pollen extract of known botanical origin. The botanical origin of the pollen was determined by palynological analysis, and then an aqueous extract was prepared. The antibacterial properties of the extract were evaluated on human infectious agents (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes) using a qualitative method (agar diffusion) and a quantitative method (minimum inhibitory and bactericide concentration) and on agricultural pathogens (Alternaria...
Tipo: Journal article Palavras-chave: Antimicrobial activity; Bee pollen; Aqueous extract.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100020
Registros recuperados: 104
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