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Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters (Bay of Biscay) ArchiMer
Labry, Claire; Delmas, Daniel; Herbland, Alain.
Previous studies conducted on the continental shelf in the Southeast Bay of Biscay influenced by Gironde waters (one of the two largest rivers on the French Atlantic coast) showed the occurrence of late winter phytoplankton blooms and phosphorus limitation of algal growth thereafter. In this context, the importance of dissolved organic phosphorus (DOP) for both algae and bacteria was investigated in 1998 and 1999 in terms of stocks and fluxes. Within the mixed layer, although phosphate decreased until exhaustion from winter to spring, DOP remained high and phosphate monoesters made up between I I to 65% of this pool. Total alkaline phosphatase activity (APA, V-max) rose gradually from winter (2-8 nM h(-1)) to late spring (100-400 nM h-1), which was mainly...
Tipo: Text Palavras-chave: Phytoplankton; Phosphate; Gironde; DOP; Bacteria; Alkaline phosphatases.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-608.pdf
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A laboratory model system for analysing microbial interactions occurring in anoxic estuarine sediments. ArchiMer
Herbert, R; Keith, S.
Defined mixed populations of Clostridium butyricum, Desulfovibrio desulfuricans and Chromatium vinosum co-existed for long periods when grown in a single stage chemostat with glucose as sole carbon source. The nature and availability of the inorganic nitrogen source exerted a major effect on carbon flow in the experimental system. The data show that both the C. butyricum and D. desulfuricans isolates can utilise NO sub(3) as an e super(-)acceptor with an increase in cell yield. Under these growth conditions the free S super(2-) levels were lower resulting in more stable mixed populations. In addition the data show that the C. butyricum produces more oxidised fermentation end-products (acetate) when NO sub(3) super(-) was available and more reduced products...
Tipo: Text Palavras-chave: Chromatium vinosum; Desulfovibrio desulfuricans; Clostridium butyricum; Biogeochemistry; Models; Bacteria; Detritus; Mineralization; Anoxic sediments; Estuarine sedimentation; Analytical techniques; Carbon cycle; Interspecific relationships; Microbiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-947.pdf
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Models of relations between bacteria and plankton, and regeneration processes in marine planktonic ecosystem. ArchiMer
Martin, Y; Lelong, P; Cauchi, B.
In order to study bacteria-plankton relationships, experimental and mathematical models were used to stimulate biological processes implicated in plankton ecosystems and to analyze community relationships. The model components are phytoplankton, zooplankton, autotrophic and heterotrophic bacteria, ciliates, nutrients, organic matter and phytoplankton antibiotics. This study shows the importance of some factors affecting bacteria communities dynamics and the bacterial activity in mineralization process. Bacterial populations seem to be controlled essentially by the concentration and chemical composition of organic substrates. Antibiosis and predation show a lesser importance on changes in bacteria communities.
Tipo: Text Palavras-chave: Biogeochemistry; Bacteria; Microorganisms; Mathematical models; Mineralization; Ecosystems; Plankton; Ecological associations.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-982.pdf
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Dynamique du phosphore et rôle comme facteur limitant de la production planctonique dans le panache de la Gironde sur le plateau continental Sud Gascogne ArchiMer
Labry, Claire.
This study was supported by the Atlantic working site of the « Programme National d’Océanographie Côtière » (PNOC, PNEC since 1999). The objectives were (1) to check the existence and to understand the mechanisms of winter phytoplankton blooms in the bay of Biscay influenced by the Gironde waters (2) to determine the limiting nutrient of the primary production associated to these blooms and to follow the seasonal evolution of the limiting nutrient, (3) to understand the consequences of this early limitation on the structuration of algal populations in spring. Since phosphorus was supposed to be the major limiting nutrient of algal growth in the Gironde plume, the different forms of the phosphorus cycle as well as the bacterial compartment, central in this...
Tipo: Text Palavras-chave: Golfe de Gascogne; Gironde; Phytoplancton; Bactéries; Phosphates; Phosphore organique; Dissous; Phosphatases; Bay of Biscay; Gironde; Phytoplankton; Bacteria; Phosphate; Dissolved organic phosphorus; Phosphatases.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00013/12463/9298.pdf
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Bacteries associees aux production de Brachionus plicatilis ArchiMer
Nicolas, Jean-louis; Joubert, M.
Bacteria associated with semi-continuous culture of Brachionus plicatilis , which is used as the first food of marine fish larvae, were counted on Zobell 2216 E medium and identified. The numbers of bacteria were very high in both Brachionus (about 10 super(11) bact. per ml of digestive content) and in water (from 1.6.10 super(6) to 4.7.10 super(7) bact. per ml). The cultures were studied at driving a time when mortalities occurred frequently. The introduction of a Pseudomonas) phenon at a proportion of about 50% to the dominant microflora persisted in time and was little influenced by diet. It has been the cause of the repression of the Vibrio, Aeromonas group which was found in an unusually small proportion. It induced a lack of microflora diversity and...
Tipo: Text Palavras-chave: Brachionus plicatilis; Rotifera; Pseudomonas; Aeromonas; Vibrio; Nutrition; Pathology; Fish culture; Fish larvae; Food organisms; Bacteria; Continuous culture; Ecological associations; Zooplankton culture.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-994.pdf
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The ocean sampling day consortium ArchiMer
Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z.; Sonnenschein, Eva C.; Cariou, Thierry; O'Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Cacador, Isabel; Canning-clode, Joao; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhaes; Luna, Gian Marco; Quero, Grazia Marina; Loescher, Carolin R.; Kremp, Anke; Delorenzo, Marie E.; Ovreas, Lise; Tolman, Jennifer; Laroche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ramos, Sandra; Magalhaes, Catarina; Jude-lemeilleur, Florence; Leopoldina Aguirre-macedo, Ma; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A.; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M.; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion M. F.; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-ezpeleta, Naiara; Berteaux-lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; Ten Hoopen, Petra; Cochrane, Guy; L'Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M.; Martin, Patrick; Jensen, Rachelle M.; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; Dos Santos, Antonina; Villar, Emilie; Pesant, Stephane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Silveira, Jorge A. Herrera; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Nyhus, Paul Anders Fronth; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N.; Gasol, Josep M.; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S.; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M.; Collins, R. Eric; Ducluzeau, Anne-lise; Martinez, Jonathan; Costello, Mark J.; Amaral-zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Gloeckner, Frank Oliver.
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.
Tipo: Text Palavras-chave: Ocean sampling day; OSD; Biodiversity; Genomics; Health Index; Bacteria; Microorganism; Metagenomics; Marine; Micro B3; Standards.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00371/48262/48647.pdf
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Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires ArchiMer
Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (>17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf
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Role des bacteries phototrophes anoxygeniques dans une partie stratifiee d'une lagune tropicale (Lagune Ebrie, Cote d'Ivoire) ArchiMer
Caumette, P.
In the deepest parts of the estuarine region of Ebrie Lagoon (Abidjan Africa, (5 degree N, 4 degree W) stratified conditions have been observed during the rainy season (May to Nov.), owing to salinity differences between oxic and anoxic layers. At the interface, 3-4 m depth, phototrophic bacteria (Chromatium, Chlorobium and Pelodictyon ) developed, forming a brown layer due to their different pigments. Their production has been estimated at 41% of the total photosynthetic production during high stratification (Sep. 1981) and their biomass was used by the copepod population for 40 to 60% of its diet. During the dry season, the water column became homogeneous and oxygen was detected to 6m depth. The brown layer of phototrophic bacteria disappeared, as well...
Tipo: Text Palavras-chave: Chlorobium; Chromatium; Primary production; Photosynthesis; Estuaries; Seasonal variations; Plankton; Stratification; Vertical distribution; Bacteria; Coastal lagoons; Trophic relationships; Biomass.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-957.pdf
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Depuration centre management. Course notes ArchiMer
O'Carroll, Terence; Boulter, Mark; Seaver, Mary; Masson, Daniel; O'Driscoll, Michael; Roden, Cillian.
The purpose of this course is to train and familiarise people, who are going to build, have just built or are about to modernise a purification plant, in the latest technology available to the industry and also to train people so that they can effectively run a depuration centre. ln general the course aims to : - Give participants practical experience of different systems prior to committing themselves to a particular system. - Enable participants to compare systems. - Provide a minimum training qualification to run an establishment which is essentially a facility to guarantee health and safety of products to the consumer. - Provide an overview from bacteria testing to plant lay out etc. The structure of the course is in modules, morning and...
Tipo: Text Palavras-chave: Shellfish depuration; Purification; Bacteria; Viruses.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00140/25082/23192.pdf
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A dual symbiosis shared by two mussel species, Bathymodiolus azoricus and Bathymodiolus puteoserpentis (Bivalvia : Mytilidae), from hydrothermal vents along the northern Mid-Atlantic Ridge ArchiMer
Duperron, Sebastien; Bergin, C; Zielinski, F; Blazejak, A; Pernthaler, A; Mckiness, Z; Dechaine, E; Cavanaugh, C; Dubilier, Nicole.
Bathymodiolus azoricus and Bathymodiolus puteoserpentis are symbiont-bearing mussels that dominate hydrothermal vent sites along the northern Mid-Atlantic Ridge (MAR). Both species live in symbiosis with two physiologically and phylogenetically distinct Gammaproteobacteria: a sulfur-oxidizing chemoautotroph and a methane-oxidizer. A detailed analysis of mussels collected from four MAR vent sites (Menez Gwen, Lucky Strike, Rainbow, and Logatchev) using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH) showed that the two mussel species share highly similar to identical symbiont phylotypes. FISH observations of symbiont distribution and relative abundances showed no obvious differences between the two host species. In...
Tipo: Text Palavras-chave: Bacteria; Phylogeny; Fluorescence in situ hybridization (FISH); Methane oxidizer; Sulfur oxidizer; 16S rRNA; Endosymbiosis.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1864.pdf
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Observation on the bacteriology of fronts : some problems of methodology ArchiMer
Floodgate, G.d.; Lochte, K.; Egan, B..
The data obtained in surveys to investigate the bacteriological aspects of the biology of two contrasting frontal systems has shown that the Inherent homeostasis and the complexity of the environment, together with the Imprecision of the data, lead to biomass values that are difficult to interpret in terms of the physical factors governing the formation of fronts. Although biochemical measurements offer some promise of providing more reliable and less confusing information, the oeed to develop high precision methods that can be made very frequently is emphasised.
Tipo: Text Palavras-chave: Bacteria; Discontinuities; Fronts; Methodology.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/00306/41742/40953.pdf
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Phased oscillations in cell numbers and nitrate in batch cultures of Alexandrium tamarense (Dinophyceae) ArchiMer
Collos, Yves; Hadjadji, Imene; Plisson, Benoit; Cecchi, Philippe; Laabir, Mohamed; Bechemin, Christian; Masseret, Estelle.
Alexandrium tamarense (M. Lebour) Balech strains isolated in spring 2007 from a single bloom in Thau lagoon have been grown in nonaxenic artificial media. For three strains showing large oscillations in biomass (crashes followed by recoveries) on a scale of several days, a significant relationship was observed between changes in cell densities (as in vivo fluorescence) and changes in nitrate concentrations. Increases in cell densities were accompanied by decreases in nitrate, while decreases in cell densities corresponded to increases in nitrate, presumably due to nitrification. Net increases in nitrate could reach up to 15 mu mol N . L(-1) . d(-1) indicating a very active nitrifying archaeal/bacterial population. However, following population crashes,...
Tipo: Text Palavras-chave: Alexandrium tamarense; Archaea; Bacteria; Decay; Growth; Nitrate; Nitrification; Oscillations.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00049/16046/13585.pdf
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Interactions microalgues-bactéries en système expérimental bispécifique : effets sur la croissance de Dunaliella sp. ArchiMer
Le Chevanton, Myriam.
The use of algae-bacteria mixed culture has been suggested to improve performance of industrial microalgae culture. The present study aims to investigate impact of selected bacteria on Dunaliella sp. growth. 97 bacteria strains were isolated from a variety of marine microalgae cultures. The diversity of strains revealed members of α-and γ-proteobacteria, Cytophaga-Flavobacterium-Bacteroides and grampositive. A two-phase approach was then implemented to select bacteria that improve Dunaliella sp. growth, using an original high-throughput experimental device. Although a majority of bacteria induced performance reduction for Dunaliella sp., some resulted in significant improvements. In nitrogenlimited batch culture, the Alteromonas sp. bacteria demonstrated a...
Tipo: Text Palavras-chave: Interactions; Microalgues; Bactéries; Dunaliella; Axénie; Interactions; Microalgae; Bacteria; Dunaliella; Axenic.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00187/29839/28291.pdf
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A test of community assembly rules using foliar endophytes from a tropical forest canopy ArchiMer
Donald, Julian; Roy, Mélanie; Suescun, Uxue; Iribar, Amaia; Manzi, Sophie; Péllissier, Léonie; Gaucher, Philippe; Chave, Jérôme.
1.Community assembly theory assumes that ecological communities are spatially delimited into patches. Within these patches, coexistence results from environmental filtering, competition, and immigration. Truly delineated communities exist in laboratory studies of microbial cultures in Petri dishes, yet empirical tests conducted in continuous environments often use patches defined by convention as opposed to realised boundaries. 2.Here we perform a test of ecological community assembly rules using foliar endophyte communities from a tropical rainforest, where leaves are considered as patches for both fungal and bacterial communities. We determined the diversity of fungal and bacterial endophytes using environmental DNA sequencing of 365 top‐canopy leaves,...
Tipo: Text Palavras-chave: Bacteria; Distribution; French Guiana; Fungi; Metabarcoding; Plant–pathogen interactions; Rainforest.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71808/70296.pdf
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Recherche et identification de bactéries pathogènes au sein d'une écloserie nurserie d'huître creuse, Crassostrea gigas, en relation avec la mortalité estivale du naissain ArchiMer
Waechter, Magali.
Summer mortality has been observed on oyster spat Crassostrea gigas since 1991 on french coasts. In our study, we deseribed symptoms observed during the mortality, especially adductor muscle weakness and we isolated strains on Zobell medium and TCBS from moribund spat. Strains isolated during a high mortality (60 %) were selected by experimental pathology on spat. Inoculation by bath led us to select a pathogenic strain (TNEMF6) for oyster spat. Phenotypic identification, phylogenetic study on 16S rDNA and quantitative DNA/DNA hybridisation showed that this strain belongs to Vibrio splendidus biovar II. Phenotyping and ribotyping with strains isolated during the study showed that only TNNIII7 and TNMB5 are similar to the pathogenic strain TNEMF6....
Tipo: Text Palavras-chave: Mortalité estivale; Naissain; Crassostrea gigas; Ecloserie; Nursery; France; Bactériologie; Bactérie; Vibrio; Vibrio splendidus biovar II; Summer mortality; Oyster spat; Crassostrea gigas; Hatchery; Nursery; France; Bacteriology; Bacteria; Vibrio; Vibrio splendidus biovar II.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00211/32226/30682.pdf
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L'ionisation des produits de la pêche ArchiMer
Kodo, Jean-louis.
Fishes and fishery products are seafoods which have a nutritive value with a high proteins rate. Fishery products ionization has four steps: the radappertization (sterilization of the seafood), the radicidation (destruction of the pathogen germs), the radurization (food conservation and microbial flora reduction), the desinsectization (insects and parasites elimination). Fishery products quality control is very important
Tipo: Text Palavras-chave: Legislation; Microbial contamination; Marine fish; Disinfection; Pathogens; Sterilization; Irradiation; Ionizing radiation; Fish inspection regulations; Quality control; Seafood; Processed fishery products; Bacteria.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-649.pdf
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Caractérisation de la virulence d'une souche de Vibrio aestuarianus, pathogène de l'huître Crassostrea gigas ArchiMer
Labreuche, Yannick.
In France. shellfish production is a traditional. well-established industry. mainly based upon commercial farming of the Pacifie oyster. Crassostrea gigas. Since the 1980's, annual mass mortalities of C. gigas have been reported during summer. Several studies on this subject have demonstrated that these mortality outbreaks resulted from complex interactions between the physiologieal andJor genetic status of the oysters. environmental factors, and one or more infectious agents, among which are bacteria of the genus Vibrio.
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarlanus; Pathogénicité; Produits extracellulaires; Métalloprotéase; Hémocytes; Interactions hôte/pathogène; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Pathogenicity; Extracellular products; Metalloprotease; Hemocytes; Host/pathogen interactions.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00034/14561/11859.pdf
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La qualité des eaux dans l'Estuaire de la Gironde. 1- Traitement des données. 2 - Synthèse ArchiMer
Maurice, Laurence.
The main problem of the Girond estuary is a pollution by the cadmium. During this last ten years, the good quality of the water is proved by the analysis of the main physico - chemical and biochemical parameters and bacteria. But since 1989, straight to the Blayais power station, the BOD5 varied between 2 and 3,5 mg.l-1 and a diminution of the dissolved oxygen has been observed; it's probably caused by the accumulation of biodegradable organic matter in the maximum turbidity zone during years characterized by low river flows. For average river flow, urban discharges takes part for 20 % in total fluxes of BD05; in 1991, this part attempts 35 %. During the last 7 years, the pluriannual evolution of the nitrate concentrations measured in the Garonne river...
Tipo: Text Palavras-chave: Oxygène; Sels nutritifs; Matière organique; Bactéries; Apports; Estuaire Gironde; Oxygen; Nutrients; Organic matter; Bacteria; Fluxes; Gironde estuary.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00037/14855/12178.pdf
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Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough ArchiMer
Toffin, Laurent; Bidault, Adeline; Pignet, Patricia; Tindall, Brian; Slobodkin, Alexander; Kato, Chiaki; Prieur, Daniel.
A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the...
Tipo: Text Palavras-chave: Phylogeny; Bacteria; Microbiology; Deep sediment layer.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-590.pdf
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Dual symbiosis in a Bathymodiolus sp mussel from a methane seep on the gabon continental margin (southeast Atlantic): 16S rRNA phylogeny and distribution of the symbionts in gills ArchiMer
Duperron, Sebastien; Nadalig, Thierry; Caprais, Jean-claude; Sibuet, Myriam; Fiala Medioni, Aline; Amann, Rudolf; Dubilier, Nicole.
Deep-sea mussels of the genus Bathymodiolus (Bivalvia: Mytilidae) harbor symbiotic bacteria in their gills and are among the dominant invertebrate species at cold seeps and hydrothermal vents. An undescribed Bathymodiolus species was collected at a depth of 3,150 m in a newly discovered cold seep area on the southeast Atlantic margin, close to the Zaire channel. Transmission electron microscopy, comparative 16S rRNA analysis, and fluorescence in situ hybridization indicated that this Bathymodiolus sp. lives in a dual symbiosis with sulfide- and methane-oxidizing bacteria. A distinct distribution pattern of the symbiotic bacteria in the gill epithelium was observed, with the thiotrophic symbiont dominating the apical region and the methanotrophic symbiont...
Tipo: Text Palavras-chave: Gabon; Southeast Atlantic; Methane seep; Hydrothermal vent; Deep sea; RRNA analysis; Methane concentration; Gill epithelium; Symbiosis; Bacteria; Mytilidae.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1266.pdf
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